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| 1 | 3-32 Reproductive Growth Index Analysis of a Heavy Ions-induced Mutant of Arabidopsis thaliana显示文摘Due to their unique advantages in physics and biology, heavy ion beams, with high mutation rate and widemutation spectrum, have been widely used in plant breeding as a novel and efficient physical mutagen. In this work,Arabidopsis thaliana (197#), which displayed decreased fertility, was induced by carbon ion beams accelerated bythe Heavy Ion Research Facility in Lanzhou (HIRFL). In order to determine the discrepancies between WT andmutant plants, a series of development indexes were analyzed during the reproductive growth stage. | Du Yan Zhou Libin Yu Lixia Luo Shanwei Feng Hui Li Wenjian | 2014 | IMP & HIRFL Annual Report2014，，1： | 0 |
| 2 | 3-33 Dose Effects of Neon Irradiation on Root and Hypocotyl Growth of Arabidopsis thaliana显示文摘Arabidopsis thaliana has many advantages for plant research, including a short generation time, small size,large number of offspring, and a relatively small nuclear genome. These advantages promoted deepen scientificinvestigation for various biological issues and characterized many genes using Arabidopsis thaliana[1].In this study, dry seeds of Arabidopsis thaliana were irradiated by neon ion beams from 100 to 500 Gy. Thenabout 10 dry seeds were sown on Murashige and Skoog medium, each treatment group repeated for three times,and all the culture dishes were grown in the culture rooms with the condition of 22?C under 18 h-light/6 h-darkcycle. Image J software was used to measure the length of roots and hypocotyl from the digital photos taken onthe 7th day after sowing. | Yu Lixia Luo Shanwei Du Yan Feng Hui Zhou Libin Li Wenjian | 2014 | IMP & HIRFL Annual Report2014，，1： | 0 |
| 3 | 3-34 Effect of γ ray Irradiation on the Root Growth of Arabidopsis Thaliana显示文摘Arabidopsis thaliana is a common research plant of molecular and cell biological research which has short lifecycle and huge amount of seeds[1]. Gamma ray is one of the important physical mutagens which is widely used inplant genetics and breeding research[2].The purpose of this study is to investigate the dose-effect of ray radiations on the root development ofArabidopsis.Dry seeds were irradiated by rays with the dose range from 100 to 1 600 Gy. | Luo Shanwei Yu Lixia Du Yan Feng Hui Zhou Libin Li Wenjian | 2014 | IMP & HIRFL Annual Report2014，，1： | 0 |
| 4 | 碳离子束辐射对大豆当代效应研究显示文摘Heavy-ion beam irradiation,as a powerful tool for mutation breeding,plays important rools for functional genomics studies in the post-genome era.In soybean,to screen the mutants with desirable agricultural characteristics can promote the production and development of China’s soybean industry in the future.In the present study,the seeds of cultivar Williams 82 were exposed to carbon-ion beam irradiation(Energy:80.55 MeV/u;Dose:0,60,80,100,120,150 and 180 Gy)that produced by the Heavy Ion Research Facility in Lanzhou(HIRFL).Biological endpoints including seedling rate,plant height and the phenotype were analyzed in this research. | Du Yan Yu Lixia Chen Xia Cui Tao Luo Shanwei Li Wenjian Zhou Libin | 2017 | IMP & HIRFL Annual Report2017，，1： | 0 |
| 5 | 4-35 Identification of Mutant Gene Induced by Carbon Ion Beams in Arabidopsis by Whole Genome Re-sequencing and Rough Map-based Cloning显示文摘In recent years,heavy ion beams have been recognized as an effective and efficient physical mutagen due to their capacity to induce mutations with high frequency and broad spectrum.Nowadays,it is not so difficult to produce various mutants in plants.However,to mine the responsible mutated genes has been an important challenge.For mutation isolation,map-based cloning is one of the major traditional ways to isolate the mutant genes that control traits of interest in forward genetics studies.However,the process of map-based cloning is usually complicated and time-consuming. | Du Yan Li Wenjian Yu Lixia Luo Shanwei Feng Hui Chen Yuze Mu Jinhu Cui Tao Chen Xia Zhou Libin | 2016 | IMP & HIRFL Annual Report2016，，1： | 0 |
| 6 | 4-42 Study on the Mechanism of Flower Pigment Mutation Induced by Carbon Ions in Geranium(Pelargonium×hortorum)显示文摘Flower color as the important economic indicator for ornamental plants has become the primary target for ornamental improving and breeding.Heavy ion mutation technology as a unique and efficient mutagen has been widely used in germplasm innovation and plant breeding.In our study,150 young shoots of Pelargonium were exposed to 80 MeV/u carbon ions and finally one stable genetic flower color mutant was obtained at dosage of 30 Gy.Comparing with wild type,the physiological indexes of mutant displayed significant differences.The color of petal in mutant was changed from red to light pink,and the color of peduncle and torus was changed from green to red,as well as the pistils and stamens’color were changed from yellow green to purplish red. | Yu Lixia Zhou Libin Du Yan Luo Shanwei Feng Hui Mu Jinhu Chen Yuze Li Wenjian | 2016 | IMP & HIRFL Annual Report2016，，1： | 0 |
| 7 | 4-44 Phenotype Screening of Arabidopsis thaliana Irradiated by Carbon Ion Beams Based on High-throughput Imaging Technique显示文摘In recent years,with the development of robotics,sensors and kinds of imaging apparatuses,researchers have developed varieties of automation,high precision and throughput phenotype analysis platforms by ceaselessly hard working and exploring[1,2].Although these platforms have improved the development of phenomics to some degree,there are still some problems in need of resolution.For example,researchers have not yet carried out an effective evaluation of the relationship between plant phenotype and genotype.Certainly,it is the key to solve these problems that how to analyze thoroughly and availably the“big data”obtained from the“high throughput”of the phenotypic analysis platform[3].Based on these issues,we attempted to analyze the huge amounts of data produced by Scanalyzer HTS,and summed up a set of research methods to analyze phenomics data. | Mu Jinhu Chen Yuze Du Yan Yu Lixia Yang Jiangyan Luo Shanwei Feng Hui Cui Tao Chen Xia Li Wenjian Zhou Libin | 2016 | IMP & HIRFL Annual Report2016，，1： | 0 |
| 8 | 3-34 Gene Mining: Association Analysis of the Whole Genome Re-sequencing and the Map-based Cloning显示文摘Since the whole genome sequence of Arabidopsis thaliana ecotype Columbia (Col-0) was published in 2 000, the focus of genomics study had turned from structural genomics to functional genomics era[1]. In the post genomics era, mutants play an important role for gene function exploring. Therefore, acquiring various variants and studying on them are useful for understanding the mechanism of plant development and molecular design for plant breeding. | Du Yan Zhou Libin Yu Lixia Luo Shanwei Feng Hui Mu Jinhu Chen Yuze Li Wenjian | 2015 | IMP & HIRFL Annual Report2015，，1： | 0 |
| 9 | 3-35 ISSR Assay of M2 Mutants of Lotus japonicus Induced by Carbon Ion Beam Irradiations显示文摘To investigate the DNA polymorphism of M2 plants of Lotus japonicus induced by heavy ion beam, dry seeds were irradiated by carbon ion beams accelerated by Heavy Ion Research Facility in Lanzhou (HIRFL). Inter simple sequence repeat (ISSR) assay were utilized to explore the DNA variation of M2 plants irradiated by 400 Gy carbon ion beams. ISSR technique is an effective molecular marker method based on polymerase chain reaction amplification of template DNA by a single primer with 1626 bp[1]. ISSR can detect the differences of genetic structure of various life materials rapidly. | Luo Shanwei Du Yan Yu Lixia Feng Hui Chen Yuze Mu Jinhu Chen Xia Zhou Libin Li Wenjian | 2015 | IMP & HIRFL Annual Report2015，，1： | 0 |
| 10 | 3-36 Optimization of ISSR-PCR System for the Mutants of Arabidopsis thaliana Induced by Carbon Ion Irradiations显示文摘Arabidopsis thaliana is one of the most commonly used model plants due to its short life cycle and concise genome.As a novel physical mutagen, heavy ion beams have been widely used in plant breeding. The most significant advantages of heavy ion beams are the higher linear energy transfer (LET) and relative biological effectiveness (RBE), inducing a broad spectrum of mutation phenotypes even at low irradiation[1]. In order to investigate the effects on DNA behavior of Arabidopsis thaliana mutants induced by carbon ion irradiations, inter-simple sequence repeat (ISSR), a DNA-based molecular marker technique, was performed to assess the genetic polymorphism. | Feng Hui Zhou Libin Du Yan Luo Shanwei Chen Yuze Mu Jinhu Yu Lixia Li Wenjian | 2015 | IMP & HIRFL Annual Report2015，，1： | 0 |
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