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| 1 | Pentoxifylline 通过 TNF- 合成和 TGF-1 基因表示的下面规定改进肝新生显示文摘 AIM:To investigate the mechanism of pentoxifylline(PTX)improvement in liver regeneration.RESULTS:Rats were randomized into 4 groups:Control rats;Sham-sham-operation rats;Saline-70% hepatectomy plus saline solution;PTX-70%hepatectomy plus PTX.At 2 and 6 h after hepatectomy,aspartate aminotransferase,alanine aminotransferase,tumor necrosis factor(TNF)-α and interleukin-6(IL-6)serum and hepatic tissue levels were determined.Tumor growth factor(TGF)-β1 gene expression in liver tissue was evaluated 24 h after hepatectomy by quantitative reverse transcriptase polymerase chain reaction analysis.Proliferation was analyzed by mitotic index and proliferating cell nuclear antigen(PCNA)staining 48 h after hepatectomy.RESULTS:TNF-α and IL-6 serum levels increased at 2 and 6 h after hepatectomy.At 2 h after hepatectomy serum PTX was reduced but not hepatic levels of TNF-α and IL-6.A decrease in liver TGF-β1 gene expression and an increase in mitotic index and PCNA after hepatectomy were observed in the PTX treatment group in comparison to the saline group.CONCLUSION:PTX improves liver regeneration by a mechanism related to down regulation of TNF-α production and TGF-β1 gene expression. | Rodrigo Bronze Martino Ana Maria Mendona Coelho Márcia Saldanha Kubrusly Regina Leito Sandra Nassa Sampietre Marcel Cerqueira Cesar Machado Telesforo Bacchella Luiz Augusto Carneiro D’Albuquerque | 2012 | World Journal of Gastrointestinal Surgery2012，4，6： | 2 |
| 2 | ApoB genehaplo type is associated with lipid profile of higher risk forcoronary heart disease in Caucasian Brazilian men 显示文摘 | Machado M O H irata M H Bertolami M C | 2001 | J Clin LabAnal2001，15，1： | 1 |
| 3 | Defunctioning stoma in low anterior resection with colonic pouch for rectal cancer:a compari-son between two hospitals with a differentpolicy 显示文摘 | Machado M Hallbook O Goldman S et al | 2002 | Dis Colon Rec-tum2002，45，7： | 1 |
| 4 | Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy显示文摘AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic lesions.METHODS:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon tumors.eUBM was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM imaging.The ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM images.During image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall anatomy.During the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon wall.Once the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and eosin.Colon images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion progression.RESULTS:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and thus,only three were subjected to the third eUBM/colonoscopy imaging acquisition(the third time-point).eUBM was able to detect the four layers in healthy segments of colon:the mucosa(the first hyperechoic layer moving away from the mini-probe axis),followed by the muscularis mucosae(hypoechoic),the submucosa(the second hyperechoic layer)and the muscularis externa(the second hypoechoic layer).Hypoechoic regions between the mucosa and the muscularis externa layers represented lymphoid infiltrates,as confirmed by the corresponding histological images.Pedunculated tumors were represented by hyperechoic masses in the mucosa layer.Among the lesions that decreased in size between the first and third time-points,one of the lesions changed from a mucosal hyperplasia with ulceration at the top to a mucosal hyperplasia with lymphoid infiltrate and,finally,to small signs of mucosal hyperplasia and lymphoid infiltrate.In this case,while lesion regression and modification were observable in the eUBM images,colonoscopy was only able to detect the lesion at the first and second time-points,without the capacity to demonstrate the presence of lymphoid infiltrate.Regarding the lesions that increased in size,one of them started as a small elevation in the mucosa layer and progressed to a pedunculated tumor.In this case,while eUBM imaging revealed the lesion at the first time-point,colonoscopy was only able to detect it at the second time-point.All colonic lesions(tumors,lymphoid infiltrate and mucosal thickening)were identified by eUBM,while colonoscopy identified just76%of them.Colonoscopy identified all of the colonic tumors but failed to diagnose lymphoid infiltrates and increased mucosal thickness and failed to differentiate lymphoid infiltrates from small adenomas.During the observation period,most of the lesions(approximately67%)increased in size,approximately 14%remained unchanged,and 19%regressed.CONCLUSION:Combining eUBM with colonoscopy improves the diagnosis and the follow-up of mouse colonic lesions,adding transmural assessment of the bowel wall. | Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado | 2013 | World Journal of Gastroenterology2013，19，44： | 1 |
| 5 | Constitutive Model for Municipal Solid Waste 显示文摘 | Machado S L Carvalho M F Vilar O M | 2002 | Journal of Geotechnical and Geoenviron-mental Engineering2002，128，11： | 1 |
| 6 | Defunctioning stoma in low anterior resection with colonic pouch for rectal cancer:a comparison between two hospitals with a different policy显示文摘 | Machado M Hallb(o)(o)k O Goldman S | 2002 | Dis Colon Rectum2002，45，7： | 1 |
| 7 | Defunctioning stoma in low anterior resection with colonic pouch for rectalcancer:a comparison between two hospitals with a different policy显示文摘 | Machado m Halbook O Goldman S | 2002 | Dis Rectum Colon2002，45，7： | 1 |
| 8 | Defanctioning stoma in low anterior resection with colonic pouch for rectal cancer:a comparison between two hospitals with a different policy显示文摘 | Hallbook O Goldman S | 2002 | Dis Colon Rectum2002，45，7： | 1 |
| 9 | Features of In Vitro Ultrasound Biomicroscopic Imaging and Colonoscopy for Detection of Colon Tumor in Mice显示文摘 | Kelly Z. Alves Helena L. Borges Rossana C. Soletti Anália L.P. Viana Lorena I. Petrella M?nica Soldan Vera L. Chagas Alberto Schanaider Jo?o C. Machado | 2011 | Ultrasound in Medicine & Biology2011，，12： | 1 |
| 10 | Antimicrobial peptides and immunolocalization of a LTP in vigna unguiculata seeds 显示文摘 | CARVALHO A O MACHADO O L T da CUNHA M | 2001 | Plant Physiogy and Biochemistry2001，39，： | 1 |
| 11 | The machinability of nickel-based alloys ; a review 显示文摘 | Ezugwu E O Wang Z M Machado A R | 1999 | Journal of Materials Pro- cessing Technology1999，86，： | 1 |
| 12 | The Machinability of Nickel-based Alloys: a Review 显示文摘 | Ezugwu E O Wang Z M Machado A R | 1999 | Journal of Materials Processing Technology1999，86，： | 1 |
| 13 | Defunctioning stoma in low anterior resection with colonic pouch for rectal cancer:a comparison between two hospitals with a different policy显示文摘 | Machado M Hallbook O Goldman S | 2002 | Dis Colon Rectum2002，45，7： | 1 |
| 14 | Radiofrequency ablation of liver metastasis from ovarian adenocarcinoma:case report and literature review显示文摘 | Bojalian M O Machado G R Swensen R | 2004 | Gynecol Oncol2004，93，2： | 1 |
| 15 | ApoB gene haplo type is associated with lipid profile of higher risk for coronary heart disease in Caucasian Brazilian men 显示文摘 | Machado M O Hirata M H Bertolami M C | 2001 | J Clin Lab Anal2001，15，1： | 1 |
| 16 | Secondary particle formation in seeded suspension polymerization 显示文摘 | Goncalves O H Machado R A F de Araujo P H H Asua J M | 2009 | Polymer2009，50，2： | 1 |
| 17 | The machinability of nickel-based alloys a review显示文摘 | Ezugwu E O Wang Z M Machado A R | 1999 | Journal of Materials Processing Technology1999，86，： | 1 |
| 18 | Constitutive model for long term municipal solid waste mechanical behavior显示文摘 | MACHADO S L VILAR O M CARVALHO M F | 2008 | Computers and Geotechnies2008，35，5： | 1 |
| 19 | Defunctioning stoma inlow anterior resection with colonic pouch for rectal cancer显示文摘 | Machado M Hallbook O Goldman S | | 0，，07： | 1 |
| 20 | Defunctioning stoma in low anterior resection with colonic pouch for rectal policy显示文摘 | Machado M Hallbook O Goldman S | 2002 | Dis Colon Rectum2002，45，7： | 1 |
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