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| 1 | Stable knockdown of heparanase expression in gastric cancer cells in vitro显示文摘AIM:To develop short hairpin RNA(shRNA)against heparanase,and to determine its effects on heparanase expression and the malignant characteristics of gastric cancer cells. METHODS:Heparanase-specific shRNA was constructed and transferred into cultured the gastric cancer cell line SGC-7901.Stable subclonal cells were screened by G418 selection.Heparanase expression was measured by reverse transcriptase-polymerase chain reaction(RT-PCR),real-time quantitative PCR and Western blotting.Cell proliferation was detected by 2-(4,5-dimethyltriazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT)colorimetry and colony formation assay. The in vitro invasiveness and metastasis of cancer cells were measured by cell adhesion assay,wound healingassay and matrigel invasion assay.The angiogenesis capabilities of cancer cells were measured by tube formation of endothelial cells. RESULTS:Stable transfection of heparanase-specific shRNA,but not of scrambled shRNA and mock vector,resulted in reduced mRNA and protein levels of heparanase.The shRNA-mediated knockdown of heparanase did not affect the cellular proliferation of SGC-7901 cells.However,the in vitro invasiveness and metastasis of cancer cells were decreased after knockdown of heparanase.Moreover,transfection of heparanase-specific shRNA decreased the in vitro angiogenesis capabilities of SGC-7901 cells. CONCLUSION:Stable knockdown of heparanase can efficiently decrease the invasiveness,metastasis and angiogenesis of human gastric cancer cells.In contrast,stable knockdown of heparanase does not affect the cell proliferation. | Li-Duan Zheng Guo-Song Jiang Jia-Rui Pu Hong Mei Ji-Hua Dong Xiao-Hua Hou Qiang-Song Tong | 2009 | World Journal of Gastroenterology2009，15，43： | 9 |
| 2 | Perioperative and long-term outcome of thymectomy for myasthenia gravis: comparison of surgical approaches and prognostic analysis显示文摘 | LIU Cheng-wu LUO Meng MEI Jian-dong ZHU Yun-ke PU Qiang MA Lin CHE Guo-wei LIN Yi-dan WU Zhu WANG Yun KOU Ying-li LIU Lun-xu | 2013 | Chinese Medical Journal2013，，1： | 8 |
| 3 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 4 | Identification of Porphyra lines using computerized DNA fingerprinting显示文摘RAPD (Randomly Amplified Polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing four important groups (P. yezoensis, P. haitanensis, P. katadai var. Hemiphylla and P. digospermatangia). Eight stable and repeatable RAPD bands amplified with two primers, OPN-02 and OPJ-18, were selected for the construction of DNA fingerprinting. The RAPD results were scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. Based on these results, a model DNA fingerprint and a computerized DNA fingerprint were constructed. In the constructed DNA fingerprint, each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other. Later, a software, named as PhGI, was designed based on this DNA fingerprinting. It can be used in practical Porphyra line identification. | WangBin, Jia Jianhang, Shi Jinfeng, Chen Yihua, JinDemin, Xu Pu, Mei Junxue, Weng Manli 1. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 (E-mail: mlweng@genetics. ac. cn), China 2. Jiangsu Marine Fishery Institute, Nan | 2001 | Acta Oceanologica Sinica2001，20，3： | 7 |
| 5 | Osa-miR1873 fine-tunes rice immunity against Magnaporthe oryzae and yield traits显示文摘MicroRNAs(miRNAs)are known to fine-tune growth,development,and stress-induced responses.Osa-miR1873 is a rice-specific miRNA targeting LOC_Os05g01790.Here,we show that Osa-miR1873 fine-tunes rice immunity against Magnaporthe oryzae and yield traits via LOC_Os05g01790.Osa-miR1873 was significantly upregulated in a susceptible accession but downregulated in a resistance accession at 24 h post-inoculation(hpi)of M.oryzae.Overexpressing Osa-miR1873 enhanced susceptibility to M.oryzae and compromised induction of defense responses.In contrast,blocking Osa-miR1873 through target mimicry compromised susceptibility to M.oryzae and enhanced induction of defense responses.Altered expression of Osa-miR1873 also resulted in some defects in yield traits,including grain numbers and seed setting rate.Moreover,overexpression of the target gene LOC_Os05g01790 increased rice blast disease resistance but severely penalized growth and yield.Taken together,we demonstrate that Osa-miR1873 fine-tunes the rice immunity-growth trade-off via LOC_Os05g01790,and blocking Osa-miR1873 could improve blast disease resistance without significant yield penalty.Thus,the Osa-miR1873-LOC_Os05g01790 regulatory module is valuable in balancing yield traits and blast resistance. | Shi-Xin Zhou Yong Zhu Liang-Fang Wang Ya-Ping Zheng Jin-Feng Chen Ting-Ting Li Xue-Mei Yang He Wang Xu-Pu Li Xiao-Chun Ma Ji-Qun Zhao Mei Pu Hui Feng Yan Li Jing Fan Ji-Wei Zhang Yan-Yan Huang Wen-Ming Wang | 2020 | Journal of Integrative Plant Biology2020，62，8： | 5 |
| 6 | Cancer metabolism and tumor microenvironment:fostering each other?显示文摘The changes associated with malignancy are not only in cancer cells but also in environment in which cancer cells live.Metabolic reprogramming supports tumor cells’high demand of biogenesis for their rapid proliferation,and helps tumor cells to survive under certain genetic or environmental stresses.Emerging evidence suggests that metabolic alteration is ultimately and tightly associated with genetic changes,in particular the dysregulation of key oncogenic and tumor suppressive signaling pathways.Cancer cells activate HIF signaling even in the presence of oxygen and in the absence of growth factor stimulation.This cancer metabolic phenotype,described firstly by German physiologist Otto Warburg,ensures enhanced glycolytic metabolism for the biosynthesis of macromolecules.The conception of metabolite signaling,i.e.,metabolites are regulators of cell signaling,provides novel insights into how reactive oxygen species(ROS)and other metabolites deregulation may regulate redox homeostasis,epigenetics,and proliferation of cancer cells.Moreover,the unveiling of noncanonical functions of metabolic enzymes,such as the moonlighting functions of phosphoglycerate kinase 1(PGK1),reassures the importance of metabolism in cancer development.The metabolic,microRNAs,and ncRNAs alterations in cancer cells can be sorted and delivered either to intercellular matrix or to cancer adjacent cells to shape cancer microenvironment via media such as exosome.Among them,cancer microenvironmental cells are immune cells which exert profound effects on cancer cells.Understanding of all these processes is a prerequisite for the development of a more effective strategy to contain cancers. | Yiyuan Yuan Huimin Li Wang Pu Leilei Chen Dong Guo Hongfei Jiang Bo He Siyuan Qin Kui Wang Na Li Jingwei Feng Jing Wen Shipeng Cheng Yaguang Zhang Weiwei Yang Dan Ye Zhimin Lu Canhua Huang Jun Mei Hua-Feng Zhang Ping Gao Peng Jiang Shicheng Su Bing Sun Shi-Min Zhao | 2022 | Science China(Life Sciences)2022，65，2： | 5 |
| 7 | A New Sesquiterpene Lactone from Ainsliaea bonatii显示文摘A new sesquiterpene lactone, Ainsliaolide A, was isolated from Ainsliaea bonatii. The structure was determined on the basis of spectral data. | Jian Xin PU Jing Feng ZHAO Xiao Dong YANG Shuang Xi MEI Hong Bin ZHANG Liang LI School of Pharmacy, Yunnan University, Kunming 650091 | 2004 | Chinese Chemical Letters2004，15，12： | 4 |
| 8 | Regioselective enzymatic acylation of troxerutin in nonaqueous medium显示文摘A series of monosubstituted troxerutin esters have been synthesized by enzyme-catalyzed regioselective acylation of troxerutin in nonaqueous medium.Using divinyl dicarboxylates(CH_2=CH-OOC-(CH_2)_n-COO-CH=CH_2,n = 2,3,4,7,8,11) featuring different chain length as acyl donors and alkaline protease from Bacillus subtilis as catalyst,troxerutin was regioselective acylated at B' ethoxyl group.The results indicated that the regioselectivity of the enzyme-catalyzed acylation was not affected by the chain lengt... | Xiao, Yong Mei Mao, Pu Zhao, Zhen Yang, Liang Ru Lin, Xian Fu | 2010 | Chinese Chemical Letters2010，21，1： | 3 |
| 9 | Osa-miR167d facilitates infection of Magnaporthe oryzae in rice显示文摘MicroRNAs(miRNAs)play important roles in rice response to Magnaporthe oryzae,the causative agent of rice blast disease.Studying the roles of rice miRNAs is of great significance for the disease control.Osa-miR167d belongs to a conserved miRNA family targeting auxin responsive factor(ARF)genes that act in developmental and stress-induced responses.Here,we show that OsamiR167d plays a negative role in rice immunity against M.oryzae by suppressing its target gene.The expression of Osa-miR167d was significantly suppressed in a resistant accession at and after 24 h post inoculation(hpi),however,its expression was significantly increased at 24 hpi in the susceptible accession upon M.oryzae infection.Transgenic rice lines over-expressing Osa-miR167d were highly susceptible to multiple blast fungal strains.By contrast,transgenic lines expressing a target mimicry to block OsamiR167d enhanced resistance to rice blast disease.In addition,knocking out the target gene ARF12 led to hypersusceptibility to multiple blast fungal strains.Taken together,our results indicate that Osa-miR167d negatively regulate rice immunity to facilitate the infection of M.oryzae by downregulating ARF12.Thus,Osa-miR167d-ARF12 regulatory module could be valuable in improvement of blast-disease resistance. | Zhi-Xue Zhao Qin Feng Xiao-Long Cao Yong Zhu He Wang Viswanathan Chandran Jing Fan Ji-Qun Zhao Mei Pu Yan Li Wen-Ming Wang | 2020 | Journal of Integrative Plant Biology2020，62，5： | 3 |
| 10 | High expression of anti-apoptotic protein Bcl-2 is a good prognostic factor in colorectal cancer: Result of a metaanalysis显示文摘AIM To systematically evaluate the prognostic-predictive capability of Bcl-2 in colorectal cancer(CRC).METHODS A systematic literature search was conducted using Pub Med,Web of Science and EMBASE databases. Any eligible study must meet the following criteria:(1) bcl-2 expression was evaluated in human CRC tissues by immunohistochemistry;(2) assessment of the relationships between bcl-2 expression and overall survival(OS),disease free survival(DFS),recurrent free survival(RFS) or clinic-pathological characteristics of CRC was included;(3) sufficient information was provided to estimate the hazard ratio(HR) or odds ratio and their 95% confidence intervals(CIs); and(4) the study was published in English. The impact of Bcl-2 expression on survival of CRC patients were evaluated through this meta-analysis.RESULTS A total of 40 eligible articles involving 7658 patients were enrolled in our final analysis. We drew the conclusion that Bcl-2 high expression was significantly correlated with favorable OS(pooled HR = 0.69,95%CI: 0.55-0.87,P = 0.002) and better DFS/RFS(pooled HR = 0.65,95%CI: 0.50-0.85,P = 0.001). Additionally,the subgroup analysis suggested that Bcl-2 overexpression was significantly associated withprognosis(OS) especially in patients came from Europe and America but not Asian and patients who did not receive any adjuvant therapy before surgery. Finally,our present results indicated that expression of bcl-2 protein was associated with high differentiation grade and A/B Ducks' stage. CONCLUSION Bcl-2 high expression was significantly correlated with favorable OS and better DFS/RFS. Hence,we propose that Bcl-2 may be a valuable prognostic-predictive marker in CRC. | Qi Huang Shu Li Pu Cheng Mei Deng Xin He Zhen Wang Cheng-Hui Yang Xiao-Ying Zhao Jian Huang | 2017 | World Journal of Gastroenterology2017，23，27： | 3 |
| 11 | A new phenylethanoid glycoside from Isodon sculponeatus显示文摘新 phenylethanoid glycoside , sculponiside ( 1 )从 Isodon sculponeatus ( Vaniot )的天线部分被孤立名声与六已知的混合物 martynoside ( 2 )一起, verbascoside ( 3 ),(+) -hydroxypinoresinol-8-O--d-glucoside ( 4 ), cedrusin ( 5 ), 7-megastigmene-3S,5R,6R,7E,9S-tetrol ( 6 )和 4-oxo--ionol--d-glucopyranoside ( 7 )。他们的化学结构从物理化学的数据并且由酸的水解作用被阐明。 | Li Mei Li Jian Xin Pu Wei Lie Xiao Han Dong Sun | 2011 | Chinese Chemical Letters2011，22，8： | 2 |
| 12 | On-demand assembly of polymeric nanoparticles for longer-blood-circulation and disassembly in tumor for boosting sonodynamic therapy显示文摘Sonodynamic therapy (SDT) is one of the promising strategies for tumor therapy, but its application is usually hindered by fast clearance in blood-circulation, abnormal tumor microenvironment, and inefficient generation of reactive oxygen species. To solve these problems, we proposed an on-demand assembly-disassembly strategy, where the assembly is favorable for longer-blood-circulation and then the disassembly in tumor is favorable for boosting SDT. Hematoporphyrin monomethyl ether (HMME) as the model of organic sonosensitizers were conjugated with hyaluronic acid (HA). Then HA-HMME was mixed with catalase (CAT) and assembled into polymeric nanoparticles (CAT@HA-HMME NPs) with size of ~80 nm. CAT@HA-HMME NPs exhibit good biocompatibility and a longer blood half-time (t1/2 = 4.17 h) which is obviously longer than that (~0.82 h) of HMME molecules. After HA receptor-mediated endocytosis of cancer cells, CAT@HA-HMME NPs can be cleaved by endogenous hyaluronidase, resulting in the on-demand disassembly in tumor to release HA-HMME molecules and CAT. The CAT catalyzes the endogenous H_(2)O_(2) into O_(2) to relieve the hypoxic microenvironment, and the released HA-HMME exhibits a higher ROS generation ability, greatly boosting SDT for the inhibition of tumor growth. Therefore, the on-demand assembly-disassembly strategy may provide some insight in the design and development of nanoagents for tumor therapy. | Mei Wen Nuo Yu Shiwen Wu Mengmeng Huang Pu Qiu Qian Ren Meifang Zhu Zhigang Chen | 2022 | Bioactive Materials2022，7，12： | 2 |
| 13 | Overproduction of OsRACK1A,an effector-targeted scaffold protein promoting OsRBOHB-mediated ROS production,confers rice floral resistance to false smut disease without yield penalty显示文摘Grain formation is fundamental for crop yield but is vulnerable to abiotic and biotic stresses.Rice grain production is threatened by the false smut fungus Ustilaginoidea virens,which specifically infects rice floral organs,disrupting fertilization and seed formation.However,little is known about the molecular mechanisms of the U.virens-rice interaction and the genetic basis of floral resistance.Here,we report that U.virens secretes a cytoplasmic effector,UvCBP1,to facilitate infection of rice flowers.Mechanistically,UvCBP1 interacts with the rice scaffold protein OsRACK1A and competes its interaction with the reduced nicotinamide adenine dinucleotide phosphate oxidase OsRBOHB,leading to inhibition of reactive oxygen species(ROS)production.Although the analysis of natural variation revealed no OsRACK1A variants that could avoid being targeted by UvCBP1,expression levels of OsRACK1A are correlated with field resistance against U.virens in rice germplasm.Overproduction of OsRACK1A restores the OsRACK1A-OsRBOHB association and promotes OsRBOHB phosphorylation to enhance ROS production,conferring rice floral resistance to U.virens without yield penalty.Taken together,our findings reveal a new pathogenic mechanism mediated by an essential effector from a flower-specific pathogen and provide a valuable genetic resource for balancing disease resistance and crop yield. | Guo-Bang Li Jia-Xue He Jin-Long Wu He Wang Xin Zhang Jie Liu Xiao-Hong Hu Yong Zhu Shuai Shen Yi-Fei Bai Zong-Lin Yao Xin-Xian Liu Jing-Hao Zhao De-Qiang Li Yan Li Fu Huang Yan-Yan Huang Zhi-Xue Zhao Ji-Wei Zhang Shi-Xin Zhou Yun-Peng Ji Mei Pu Peng Qin Shigui Li Xuewei Chen Jing Wang Min He Weitao Li Xian-Jun Wu Zheng-Jun Xu Wen-Ming Wang Jing Fan | 2022 | Molecular Plant2022，15，11： | 2 |
| 14 | Exosomes secreted from cardiomyocytes suppress the sensitivity of tumor ferroptosis in ischemic heart failure显示文摘Heart failure(HF)patients in general have a higher risk of developing cancer.Several animal studies have indicated that cardiac remodeling and HF remarkably accelerate tumor progression,highlighting a cause-and-effect relationship between these two disease entities.Targeting ferroptosis,a prevailing form of non-apoptotic cell death,has been considered a promising therapeutic strategy for human cancers.Exosomes critically contribute to proximal and distant organ-organ communications and play crucial roles in regulating diseases in a paracrine manner.However,whether exosomes control the sensitivity of cancer to ferroptosis via regulating the cardiomyocyte-tumor cell crosstalk in ischemic HF has not yet been explored.Here,we demonstrate that myocardial infarction(MI)decreased the sensitivity of cancer cells to the canonical ferroptosis activator erastin or imidazole ketone erastin in a mouse model of xenograft tumor.Post-MI plasma exosomes potently blunted the sensitivity of tumor cells to ferroptosis inducers both in vitro in mouse Lewis lung carcinoma cell line LLC and osteosarcoma cell line K7M2 and in vivo with xenograft tumorigenesis model.The expression of miR-22-3p in cardiomyocytes and plasma-exosomes was significantly upregulated in the failing hearts of mice with chronic MI and of HF patients as well.Incubation of tumor cells with the exosomes isolated from post-MI mouse plasma or overexpression of miR-22-3p alone abrogated erastin-induced ferroptotic cell death in vitro.Cardiomyocyte-enriched miR-22-3p was packaged in exosomes and transferred into tumor cells.Inhibition of cardiomyocyte-specific miR-22-3p by AAV9 sponge increased the sensitivity of cancer cells to ferroptosis.ACSL4,a pro-ferroptotic gene,was experimentally established as a target of miR-22-3p in tumor cells.Taken together,our findings uncovered for the first time that MI suppresses erastin-induced ferroptosis through releasing miR-22-3p-enriched exosomes derived from cardiomyocytes.Therefore,targeting exosome-mediated cardiomyocyte/tumor pathological communication may offer a novel approach for the ferroptosis-based antitumor therapy. | Ye Yuan Zhongting Mei Zhezhe Qu Guanghui Li Shuting Yu Yingqi Liu Kuiwu Liu Zhihua Shen Jiaying Pu Yanquan Wang Changhao Wang Zhiyong Sun Qian Liu Xiaochen Pang Ao Wang Zijing Ren Tong Wang Ying Liu Jinhuan Hong Jiajie Xie Xin Li Zhonghua Wang Weijie Du Baofeng Yang | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 2 |
| 15 | Laparoscopic versus open pyeloplasty for ureteropelvic junction obstruction in children:a systematic review and meta-analysis显示文摘 | Mei H Pu J Yang C | 2011 | J Endourol2011，25，5： | 1 |
| 16 | Molecular cloning of the eDNA encoding laccase from Trametes versicolor and heterologous expression in Pichia methanolica 显示文摘 | GUO Mei LU Fu-ping PU Jun | 2005 | Appl Mierobiol Biotechnol2005，69，2： | 1 |
| 17 | A novel method for troubleshooting vascular injury during anatomic thoracoscopic pulmonary resection without conversion to thoracotomy 显示文摘 | Mei J Pu Q Liao H | 2013 | Surg Endosc2013，27，2： | 1 |
| 18 | Value of video-assisted thorac- ic surgery core needle biopsy in the selection of surgical ap- proaches for indeterminate pulmonary nodules 显示文摘 | Liao H Pu Q Mei J | 2013 | Ann Thorac Surg2013，95，2： | 1 |
| 19 | Molecular cloning of the cDNA encoding laccase from Trametes versicolor and ion in Pichia methanolica 显示文摘 | Mei Guo Lu Fuping Pu Jun | 2005 | Appl Microbiol Biotechnol2005，69，2： | 1 |
| 20 | Initial experience of video- assisted thoracic surgery left upper sleeve lobectomy for lung cancer: Case report and literature review显示文摘 | Mei JD Pu Q Liao H | 2012 | Thoracic Cancer2012，3，4： | 1 |
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