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您的检索式：作者名="Mengchen Jin"
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| 1 | Enzymes immobilized in wood-derived cellulose scaffold for constructing a novel modular bioreactor显示文摘Modular bioreactors can provide a flexible platform for constructing complex multi-step pathways,which may be a solution for maximizing reactions and overcoming the complexity of multi-enzyme systems.Here,we selected wood-derived cellulose scaffold as a support for enzyme immobilization and constructed the modular bioreactor.Cellulose scaffold was prepared after removing lignin from wood,followed by citric acid functionalization and the addition of glutaraldehyde finally allowed the cross-linking of enzymes.Three enzymes,horseradish peroxidase(HRP),glucose oxidase(GOD),and catalase(CAT),were separately immobilized,resulting in the immobilized enzyme amount to over 40 mg/g.The introduction of carboxyl groups from citric acid facilitated the rapid enzyme adsorption on the support surface and immobilized enzymes possess~65%expressed activity.Modular bioreactors were constructed by using the immobilized enzymes.With the immobilized HRP module,reactor showed desired catalytic performance with the phenol degradation rate of>90%.Also,a pH regulation can occur in the bioreactors for preserving enzyme activities and neutralizing acid products.In the GOD/CAT modular bioreactor,the cascade reaction with adjusting pH values can achieve a 95%yield of sodium gluconate and exhibit a favorable reusability of 5 operation cycles. | Zhihong Zhang Mengchen Jin Guiru Chen Jiandu Lei Luying Wang Jun Ge | 2023 | Green Chemical Engineering2023，4，1： | 0 |
| 2 | Swertiamarin ameliorates diet-induced obesity by promoting adipose tissue browning and oxidative metabolism in preexisting obese mice显示文摘Obesity is a risk factor for many metabolic diseases.Efficient therapeutic strategies are urgently needed.Swertiamarin(STM)prevents obesity and the associated insulin resistance and inflammation.However,the therapeutic effects of STM on preexisting obesity remain unclear.Therefore,in this study we aim to investigate the effects of STM on energy expenditure and fat browning in mice with preexisting obesity.C57BL/6J mice are fed with a high-fat diet(HFD)for 8 weeks to induce obesity and then gavaged(or not)with STM for 10 weeks.The whole-body energy metabolism of mice is examined by indirect calorimetry.The results show that after 10 weeks of treatment,STM markedly prevents HFD-induced weight gain,chronic inflammation,insulin resistance,and hepatic steatosis.STM promotes oxygen consumption and energy expenditure.The level of uncoupling protein 1 is enhanced in the brown and white adipose tissues of STM-treated mice.STM increases the phosphorylation of AMP-activated protein kinase and the expressions of genes involved in fat oxidation,reducing fat deposition in skeletal muscles.Meanwhile,STM does not affect the intestinal microbiotic composition.Overall,STM supplementation may serve as a potential therapy for obesity. | Yuqin Zhu Haoran Li Mengchen Ma Dandan Li Ouyang Zhang Suili Cai Yajiao Wang Dandan Chen Shengnan Jin Chunming Ding Liang Xu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，1： | 0 |
| 3 | Rare and undiagnosed diseases:From disease-causing gene identification to mechanism elucidation显示文摘Rare and undiagnosed diseases substantially decrease patient quality of life and have increasingly become a heavy burden on healthcare systems.Because of the challenges in disease-causing gene identification and mechanism elucidation,patients are often confronted with difficulty obtaining a precise diagnosis and treatment.Due to advances in sequencing and multiomics analysis approaches combined with patient-derived iPSC models and gene-editing platforms,substantial progress has been made in the diagnosis and treatment of rare and undiagnosed diseases.The aforementioned techniques also provide an operational basis for future precision medicine studies.In this review,we summarize recent progress in identifying disease-causing genes based on GWAS/WES/WGS-guided multiomics analysis approaches.In addition,we discuss recent advances in the elucidation of pathogenic mechanisms and treatment of diseases with state-of-the-art iPSC and organoid models,which are improved by cell maturation level and gene editing technology.The comprehensive strategies described above will generate a new paradigm of disease classification that will significantly promote the precision and efficiency of diagnosis and treatment for rare and undiagnosed diseases. | Gang Wang Yuyan Xu Qintao Wang Yi Chai Xiangwei Sun Fan Yang Jian Zhang Mengchen Wu Xufeng Liao Xiaomin Yu Xin Sheng Zhihong Liu Jin Zhang | 2022 | Fundamental Research2022，2，6： | 0 |
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