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2篇 您的检索式:作者名="Mikhail N Slipchenko"
    题名 作者 年代 出处 被引量
1Microsecond scale vibrational spectroscopic imaging by multiplex stimulated Raman scattering microscopy显示文摘Real-time vibrational spectroscopic imaging is desired for monitoring cellular states and cellular processes in a label-free manner.Raman spectroscopic imaging of highly dynamic systems is inhibited by relatively slow spectral acquisition on millisecond to second scale.Here,we report microsecond scale vibrational spectroscopic imaging by lock-in free parallel detection of spectrally dispersed stimulated Raman scattering signal.Using a homebuilt tuned amplifier array,our method enables Raman spectral acquisition,within the window defined by the broadband pulse,at the speed of 32 μs and with close to shot-noise limited detection sensitivity.Incorporated with multivariate curve resolution analysis,our platform allows compositional mapping of lipid droplets in single live cells,observation of intracellular retinoid metabolism,discrimination of fat droplets from protein-rich organelles in Caenorhabditis elegans,spectral detection of fast flowing tumor cells and monitoring drug diffusion through skin tissue in vivo.The reported technique opens new opportunities for compositional analysis of cellular compartment in a microscope setting and high-throughput spectral profiling of single cells in a flow cytometer setting.Chien-Sheng Liao Mikhail N Slipchenko Ping Wang Junjie Li Seung-Young Lee Robert A Oglesbee Ji-Xin Cheng 2015Light(Science & Applications)2015,4,1:8
2Infrared intensity in small ammonia and water clusters显示文摘Mikhail N Slipchenko Kirill E 2006Journal of Chemical Physics2006,124,24:1
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