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| 1 | Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke显示文摘Human dental pulp stem cells(hDPSCs) promote recovery after ischemic stro ke;however,the therapeutic efficacy is limited by the poor survival of transplanted cells.For in vitro expe riments in the present study,we used oxygen-glucose deprivation/reoxygenation in hDPSCs to mimic cell damage induced by ischemia/reperfusion.We found that miRNA-34a-5p(miR-34a) was elevated under oxygen-glucose deprivation/reoxygenation conditions in hDPSCs.Inhibition of miR-34a facilitated the prolife ration and antioxidant capacity and reduced the apoptosis of hDPSCs.Moreove r,dual-luciferase reporter gene assay showed WNT1and SIRT1 as the targets of miR-34a.In miR-34a knockdown cell lines,WNT1 suppression reduced cell prolife ration,and SIRT1 suppression decreased the antioxidant capacity.Togethe r,these results indicated that miR-34a regulates cell prolife ration and antioxidant stress via targeting WNT1 and SIRT1,respectively.For in vivo expe riments,we injected genetically modified hDPSCs(anti34a-hDPSCs) into the brains of mice.We found that anti34a-hDPSCs significantly inhibited apoptosis,reduced cerebral edema and cerebral infarct volume,and improved motor function in mice.This study provides new insights into the molecular mechanism of the cell prolife ration and antioxidant capacity of hDPSCs,and suggests a potential gene that can be targeted to improve the survival rate and efficacy of transplanted hDPSCs in brain after ischemic stroke. | Jianfeng Wang Peibang He Qi Tian Yu Luo Yan He Chengli Liu Pian Gong Yujia Guo Qingsong Ye Mingchang Li | 2023 | Neural Regeneration Research2023，18，9： | 1 |
| 2 | Reform of Elastic-Plastic Mechanics and Strength Design of Pipelines Based on Ideological and Political Education显示文摘Oil and gas storage and transportation safety courses are very important in oil and gas storage and transportation engineering.With the ideological and political construction of the course in the new period,the mode of professional knowledge teaching cannot meet the teaching requirements.The teaching team has been practicing in the course for many years.By inserting typical characters,typical projects,national policies and industry trends into the course,students'patriotism,academic confidence and environmental protection awareness have been cultivated,and remarkable results have been achieved.At the same time,it can provide some reference for the ideological and political education of engineering majors. | Yanfei Chen You Zong Mingchang He Chunsha Wan | 2021 | Review of Educational Theory2021，4，4： | 0 |
| 3 | MSC based gene delivery methods and strategies improve the therapeutic efficacy of neurological diseases显示文摘Mesenchymal stem cells(MSCs)are promising seed cells for neural regeneration therapy owing to their plasticity and accessibility.They possess several inherent characteristics advantageous for the transplantation-based treatment of neurological disorders,including neural differentiation,immunosuppression,neurotrophy,and safety.However,the therapeutic efficacy of MSCs alone remains unsatisfactory in most cases.To improve some of their abilities,many studies have employed genetic engineering to transfer key genes into MSCs.Both viral and nonviral methods can be used to overexpress therapeutic proteins that complement the inherent properties.However,to date,different modes of gene transfer have specific drawbacks and advantages.In addition,MSCs can be functionalized through targeted gene modification to facilitate neural repair by promoting neural differentiation,enhancing neurotrophic and neuroprotective functions,and increasing survival and homing abilities.The methods of gene transfer and selection of delivered genes still need to be optimized for improved therapeutic and targeting efficacies while minimizing the loss of MSC function.In this review,we focus on gene transport technologies for engineering MSCs and the application of strategies for selecting optimal delivery genes.Further,we describe the prospects and challenges of their application in animal models of different neurological lesions to broaden treatment alternatives for neurological diseases. | Heng Zhou Yan He Wei Xiong Shuili Jing Xingxiang Duan Zhiyun Huang Gurek SNahal Youjian Peng Mingchang Li Yaoqi Zhu Qingsong Ye | 2023 | Bioactive Materials2023，，5： | 0 |
| 4 | Compiling Textbook of Elastic-plastic Mechanics for Major of Oil and Gas Storage and Transportation Engineering显示文摘'Elastic-plastic mechanics'is a required course for engineering postgraduates in petroleum colleges and universities,such as students who major in oil and gas storage and transportation engineering.It is the basis for personnel engaged in structural safety assessment.In the past teaching process,the teaching effects of this course were unsatisfactory.There are many reasons for this phenomenon,such as the strong theoretical nature of this course,the need for a large number of formula derivation,the high requirements for students'mechanical foundation and mathematical foundation,the lack of appropriate teaching materials and the slackness of students'minds.Based on years of teaching experience,the teaching team has reformed the existing teaching materials of“Elastic-plastic mechanics”to meet the needs of petroleum colleges and universities.In this reform,we have referred to a large number of published textbooks of“Elasticplastic mechanics”and the experience of textbook construction at home and abroad.The newly compiled textbook of“Elastic-plastic mechanics”plays a certain role in improving the teaching quality of“Elastic-plastic mechanics”in petroleum colleges and universities. | Yanfei Chen Yufeng Yan Heng Ni Mingchang He Zhihao Wang Zuming Wu | 2021 | Journal of Educational Theory and Management2021，5，2： | 0 |
| 5 | The Reform of Elastic-plastic Mechanics Teaching for Petroleum Engineering Related Majors显示文摘“Elastic-plastic Mechanics”is an important course for undergraduates and postgraduates of general engineering majors.However,the theoretical derivation of equations is complex,and the connection with engineering practice is inadequate.Therefore,teaching becomes difficult and boring for a number of students.Firstly,this paper introduces the importance of Elastic-plastic Mechanics for petroleum engineering related majors.Based on the recent teaching experience,the teaching reform of Elastic-plastic Mechanics course is carried out focusing on teaching method and learning content,and a discussion teaching mode based on students’independent discussion and engineering cases is formed.Remarkable results have been achieved in improving students’learning efficiency and classroom teaching effect.Furthermore,students’comprehensive ability of independent innovation and practice is enhanced. | Yanfei Chen Tongjing Xia Ye Zhang Lulu Li Mingchang He Hong Zhang | 2020 | Review of Educational Theory2020，3，4： | 0 |
| 6 | TIME-DEPENDENT FATIGUE AND ITS MICROMECHANISM OF SUPERALLOY GH169 UNDER OXIDATION显示文摘The time-dependent fatigue and its micromechanism of superalloy GH169 at 650℃ have beenstudied.Under the same stress and duration,its cyclic life in Ar is shorter than that in air.Thedamage mechanism is mainly of the interaction between high temperature oxidation and fa-tigue.The fracture surface of GH169 specimen oxidized at 650℃ in air is revealed to be cov-ered with a dense and even oxide film,but in Ar,with uncontinuous oxide particles.As thetime prolonged,the Cr2O3 content in the surface oxides increases in air and decreases in Ar. | HE Jinrui LU Younian WANG Qingsui SU Hansheng ZHANG Xueduo WU Mingchang ZHU Qiao Beijing Institute of Aeronautical Materials,Beijing,China P.O.No.81—23,Beijing 100095,China | 1990 | Acta Metallurgica Sinica(English Letters)1990，3，5： | 0 |
| 7 | An Analysis of 1 001 Blinding Patients with Corneal Disease in 1960-1989显示文摘Purpose:To investigate the condition and change of corneal blindness (CB) in the past 30 years.Methods: 1 001 blinding patients of corneal disease were clinically analysed from 1960 to 1989,including etiology, sex, age, occupation, difference between the urban and rural areas etc.Results: The male predominated. Infection stood the first cause of blinding corneal diseases, followed by trauma,malnutrition and the others.HSV-1 keratitis was the most frequent infection in 1980s. The highest incidence of CB was 20-40 year -old. The number of CB caused by occupational truauma was more than that by ordinary trauma in 1960s,but from 1970s, the latter was more than the former. The number of CB in rural area was more than that in the urban in 1960s,but from 1970s,there was no significant difference between the two areas. The percentage of bilateral CB was gradually decreased.Conclusion: We should try to find more effective strategies to prevent and treat CB caused by HSV-1 keratitis, pay special attention to | Mingchang Zhang, Yunan He, Houren Wei, Caikeng MaiDepartment of Ophthalmology, Union Hospital of Tongji Medical University, Wuhan 430022, China | 1998 | 眼科学报1998，14，1： | 0 |
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