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| 1 | Multispecies probiotic protects gut barrier function in experimental models显示文摘AIM:To investigate the effect of the probiotic combination Lactibiane Tolerance?(LT)on epithelial barrier function in vitro and in vivo.METHODS:The effect of the multispecies probiotic LT was assessed on several models of epithelial barrier function both in vitro(in basal and inflammatory conditions)and in vivo[visceral hypersensitivity induced by chronic stress or by colonic perfusion of a fecal supernatant(FSN)from patients with irritable bowel syndrome(IBS)].In vitro,we measured the permeability of confluent T84 cell monolayers incubated with or without LT by evaluating the paracellular flux of macromolecules,in basal conditions and after stimulation with lipopolysaccharide(LPS)or with conditioned medium of colonic biopsies from IBS patients(IBS-CM).In vivo,male C57/Bl6 mice received orally NaCl or LT for 15 d and were submitted to water avoidance stress(WAS)before evaluating visceral sensitivity by measuring the myoelectrical activity of the abdominal muscle and the paracellular permeability with 51Cr-EDTA.Permeability and sensitivity were also measured after colonic instillation of FSN.Tight-junctions were assessed by immunoblotting and TLR-4 expression was evaluated by immunohistochemistry RESULTS:Incubation of T84 cell monolayers with LT in basal conditions had no significant effect on permeability(P>0.05 vs culture medium).By contrast,addition of LT bacterial bodies(LT)completely prevented the LPS-induced increase in paracellular permeability(P<0.01 vs LPS 10 ng/mL(LPS 10);P<0.01 vs LPS 100ng/mL(LPS 100),P>0.05 vs culture medium).The effect was dose dependent as addition of 109 LT bacterial bodies induced a stronger decrease in absorbance than 106 LT(109 LT+LPS 10:-20.1%±13.4,P<0.01vs LPS 10;106 LT+LPS 10:-11.6%±6.2,P<0.01 vs LPS 10;109 LT+LPS 100:-14.4%±5.5,P<0.01 vs LPS 100;106 LT+LPS 100:-11.6%±7.3,P<0.05 vs LPS 100).Moreover,the increase in paracellular permeability induced by culturing T84 cells with conditioned medium of colonic biopsies from IBS patients(IBS-CM)was completely inhibited in the presence of 109 LT(P<0.01 vs IBS-CM).LT also significantly prevented the epithelial disruption induced by intracolonic infusion of fecal supernatant from IBS patients(P<0.01 vs IBS FSN)or water avoidance stress P<0.01 vs WAS)in C57/Bl6 mice and increased the expression of occludin in vitro and in vivo,as assessed by immnunoblotting.The WAS-induced effect on visceral sensitivity was prevented by LT treatment since values obtained for all steps of colorectal distension were significantly(P<0.01)different from the WAS group.Finally,LT downregulated the response mediated through TLR-4 in vitro(decrease in tumor necrosis factorαsecretion in response to LPS:-65.8%for 109 LT and-52.5%for 106LT,P<0.01 vs LPS)and in vivo(inhibition of WAS induced an increase in TLR-4 expression in the LT treated mice colon,P<0.01 vs WAS).CONCLUSION:The probiotic LT mix prevented the disruption to the epithelial barrier induced by LPS,stress or colonic soluble factors from IBS patients and prevented visceral hypersensitivity. | Mylene Nébot-Vivinus Cherryl Harkat Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese Lara Moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 2 | Community knowledge,attitudes and practices related to schistosomiasis and associated healthcare-seeking behaviours in northern Côte d’Ivoire and southern Mauritania显示文摘Background:Among parasitic infections,schistosomiasis ranks second after malaria in terms of worldwide morbidity.Despite efforts to contain transmission,more than 230 million people are infected,of which 85%live in Sub-Saharan Africa.While the epidemiologic characteristics of schistosomiasis have been extensively studied across endemic settings,social factors have been paid less attention.The current study assesses community knowledge of schistosomiasis causes,transmission,signs,symptoms and prevention,as well as healthcare-seeking behaviours in two West African settings,with the aim of strengthening schistosomiasis control interventions.Methods:From August 2014 to June 2015,we conducted two cross-sectional surveys in Korhogo,Cote d’Ivoire and Kaedi,Mauritania.We applied a questionnaire to collect quantitative data at the household level in Korhogo(n=1456)and Kaedi(n=1453).Focus group discussions(Korhogo:n=32,Kaedi:n=32)and participatory photography(photovoice)(Korhogo:n=16,Kaedi:n=16)were conducted within the communities to gather qualitative data.In addition,semi-structured interviews were used to discuss with key informants from control programmes,nongovernmental organizations and health districts(Korhogo:n=8,Kaedi:n=7).Results:The study demonstrated that schistosomiasis is not well known by the communities;64.1%claimed to know the causes of the disease,but the reality is different.This knowledge is more from cultural than biomedical source.It was observed that social construction of the disease is different from the biomedical definition.In Korhogo,schistosomiasis was often associated with several other diseases,notably stomach ulcer and gonorrhoea.The populations believe that schistosomiasis is caused by exposure to goat or dog urine in the environment.In Kaedi,schistosomiasis is considered as a disease transmitted by environmenal elements such as sunshine and dirty water.In both settings,the care-seeking pathways were found to be strongly influenced by local customs and self-medication acquired from the informal sector.Conclusions:This study revealed that knowledge about the aetiology,transmission,symptoms,prevention and treatment of schistosomiasis among the populations in Korhogo and Kaedi is based on their local culture.Deep-rooted habits could therefore pose a significant obstacle to the elimination of schistosomiasis. | Amoin Jeanne d’Arc Koffi Mohamed Doumbia Gilbert Fokou Moussa Keita Brama Kone N’doumy Noel Abe | 2018 | Infectious Diseases of Poverty2018，7，1： | 2 |
| 3 | 原发性醛固酮增多症肾上腺静脉采血时醛固酮测量值的变异性显示文摘已有研究描述原发性醛固酮增多症(原醛)患者醛固酮浓度变异性。本研究纳入原醛患者340例,进行回顾性队列研究。受试者在三级转诊中心完成肾上腺静脉采血(adrenal venous sampling,AVS),其中116例在AVS术前数小时测定外周静脉醛固酮水平。AVS由同一介入放射科医生实施,在是否刺激状态下,双侧同时采样,每份样本采3份。主要观察指标包括(1)AVS前和AVS中醛固酮的变化;(2)3份AVS肾上腺静脉醛固酮浓度的变异性。 | Yozamp N Hundemer GL Moussa M Underhill J Fudim T Sacks B Vaidya A 袁源(翻译) 郑武洪(审校) | 2021 | 中华高血压杂志2021，29，11： | 2 |
| 4 | Fractal analysis of hydroxyapatite from nitrogen isotherms显示文摘 | E1 Shafei G M S Philip C A Moussa N A | 2004 | Journal of Colloid and Interface Science2004，277，2： | 1 |
| 5 | Seasonal, diurnal and nocturnal behaviors of lower carbonyl compounds in the urban environment of Beirut, Lebanon 显示文摘 | Moussa S G El-Fadel M Saliba N A | 2006 | Atmospheric Environment2006，40，14： | 1 |
| 6 | The Dornier Compact Delta lithotripter: the first 500 renal calculi显示文摘 | Lalak N J Moussa SA Smith G | 2002 | J Endourol2002，16，1： | 1 |
| 7 | A retrospective analysis of pathological placental implantation-site and penetration显示文摘 | Makhseed M Tomi N Moussa M | 2004 | Int J Gynacol Obstet2004，47，2： | 1 |
| 8 | Seasonal, diurnal and nocturnal behaviors of lower carbenyl compounds in the urban environment of Beirut, Lebanon 显示文摘 | Moussa S G El-Fadel M Saliba N A | 2006 | Atmos Environ2006，40，： | 1 |
| 9 | Reduction of fungi and mycotoxins formation in seeds by gamma irradiation 显示文摘 | Aziz N H Moussa L A A | 2004 | Journal of Food Safety2004，24，2： | 1 |
| 10 | Automatic Classification and3D Modeling of LiDAR Data显示文摘 | Moussa A El-Sheimy N | 2010 | International Archives ofPhotogrammetry and Remote Sensing2010，38，3： | 1 |
| 11 | Association of C-reactive protein with coronary heart disease risk factors in patients with type 2 diabetes mellitus显示文摘 | Mojiminiyi OA Abdella N Moussa MA | 2002 | Diabetes Res Clin Pract2002，58，1： | 1 |
| 12 | Experiments Involving Pool and Vapor Fires from Spills of Liquefied Natural Gas on Water显示文摘 | RA3 P P K MOUSSA A N ARACAMUDAN K | 1979 | Electromagnetic Spectrum1979，，： | 1 |
| 13 | Association of Creactive protein with coronary heart disease risk factors in patients with type 2 diabetes mellitus显示文摘 | Mojiminiyi O A Abdella N Moussa M A | 2002 | Diabetes Res Clin Pract2002，58，： | 1 |
| 14 | A retrospective analysis of pathological placental implantation site and penetration显示文摘 | Makhseed M Tomi N Moussa M | 1994 | Int J Gynaecol Obstet1994，47，2： | 1 |
| 15 | Influence of temperature on struvite precipitation by C02-degassing method 显示文摘 | Moussa S B Tlili M M Batis N | 2011 | Crystal Research and Technology2011，46，3： | 1 |
| 16 | Diagnosis of weathered coptic wall paintings in the Wadi El Natrun region, E- gypt显示文摘 | Moussa A B Kantiranis N Voudouris K S et / | 2009 | J Cuh Heri2009，10，： | 1 |
| 17 | A retrospective analysis of pathological placental implantation site and penetration显示文摘 | MAKHSEED M TOMI N MOUSSA M | 1996 | Int J Gynaecol Obstet1996，47，2： | 1 |
| 18 | Placental cytokine and chemokine production in HIV-l-infected women: trophoblast cells show a different pattern compared to cells from HIV-negative women显示文摘 | Moussa M Roques P Fievet N | 2001 | Clin Exp hnmunol2001，125，3： | 1 |
| 19 | Plasma leptin concentration in patients with type 2 di- abetes: relationship to cardiovascular diseaserisk factors andinsulin resistance显示文摘 | ABDELLA N A MOJIMINIYI O A MOUSSA M A | 2005 | Diabet Med2005，22，3： | 1 |
| 20 | Social and psychological characteristics of Kuwaiti children and adolescents with type 1 diabetes显示文摘 | Moussa MMA Alsaeid M Abdella N | 2005 | Social Science & Medicine2005，60，： | 1 |
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