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| 1 | The role of chemosensory protein 10 in the detection of behaviorally active compounds in brown planthopper, Nilaparvata lugens显示文摘Chemosensory proteins(CSPs)play important roles in insects’chemoreception,although their specific functional roles have not been fully elucidated.In this study,we conducted the developmental expression patterns and competitive binding assay as well as knock‐down assay by RNA interference both in vitro and in vivo to reveal the function of NlugCSP10 from the brown planthopper(BPH),Nilaparvata lugens(Stål),a major pest in rice plants.The results showed that NlugCSP10 messenger RNA was significantly higher in males than in females and correlated to gender,development and wing forms.The fluorescence binding assays revealed that NlugCSP10 exhibited the highest binding affinity with cis‐3‐hexenyl acetate,eicosane,and(+)‐β‐pinene.Behavioral assay revealed that eicosane displayed attractant activity,while cis‐3‐hexenyl acetate,similar to(+)‐β‐pinene significantly repelled N.lugens adults.Silencing of NlugCSP10,which is responsible for cis‐3‐hexenyl acetate binding,significantly disrupted cis‐3‐hexenyl acetate communication.Overall,findings of the present study showed that NlugCSP10 could selectively interrelate with numerous volatiles emitted from host plants and these ligands could be designated to develop slow‐release mediators that attract/repel N.lugens and subsequently improve the exploration of plans to control this insect pest. | Muhammad Irfan Waris Aneela Younas Muhammad Muzammal Adeel Shuang-Gang Duan Sundas Rana Quershi Rana Muhammad Kaleem Ullah Man-Qun Wang | 2020 | Insect Science2020，27，3： | 9 |
| 2 | Appraisal of MsepCSP14 for chemosensory functions in Mythimna separata显示文摘Chemosensory proteins (CSPs) have great contributions in performing diverse functions in insects. However, physiological appraisal of chemosensory protein genes still remains elusive in insects. We studied expression patterns and binding affinities of MsepCSP14, a chemosensory protein, in Mythimna separata. The distinct functions of MsepCSP14 were validated by employing different molecular techniques. The MsepCSP14 had high resemblance of sequence with chemosensory proteins of other insect family members. The MsepCSP14 expression was higher in antennal tissues of females than other tissues. Fluorescence binding assay validated that binding of nine out of 21 ligands to MsepCSP14 was higher at pH 7.4 than at pH 5.0. Three dimensional modeling (3D) and docking analysis predicted that amino acid residues of MsepCSP14 were involved in binding of compounds, and behavior assay displayed that adults of M. separata considerably responded to four volatiles from compounds demonstrating strong binding ability to MsepCSP14. Results of the present study suggest that MsepCSP14 is likely to mediate chemosensory functions in M. separata. | Aneela Younas Muhammad Irfan Waris Muhammad Shaaban Muhammad Tahir ul Qamar Man-Qun Wang | 2022 | Insect Science2022，29，1： | 1 |
| 3 | 0.2%高效氯氰菊酯粉剂对田间红火蚁种群数量及蚁巢迁移的影响显示文摘为科学评价触杀性粉剂对红火蚁Solenopsis invicta Buren的防治效果,利用0.2%高效氯氰菊酯粉剂对田间红火蚁蚁巢进行单蚁巢处理,分析粉剂施用剂量和次数对田间红火蚁种群数量及蚁巢迁移的影响。结果表明,触杀性粉剂处理可在短期内显著降低红火蚁的蚁巢数量和工蚁诱集数量,50 g/巢和100 g/巢处理1 d后,蚁巢数量降幅分别达55.17%和66.04%,工蚁诱集数量降幅分别达31.79%和51.95%,但5~9 d后基本恢复至处理前水平,第21天蚁巢增加率达86.21%和37.74%,持效性较差。增多粉剂施用次数同样也只能在短期内压低红火蚁的蚁巢数量和工蚁诱集数量,施用1、2和3次粉剂1 d后蚁巢数量降幅分别达61.44%、76.82%和80.79%,工蚁诱集数量降幅分别达55.40%、47.21%和28.36%,随着时间延长,蚁巢数量和工蚁数量很快恢复。虽然随着施药剂量增加,蚁巢增加率和分巢率明显降低,新蚁巢出现时间略有延迟,新蚁巢的体积明显减小,但整体来看蚁巢数量仍呈上升趋势,新蚁巢出现的距离和方位也没有明显差异。表明粉剂防治仅可在短期内表面上压低红火蚁种群数量,即使增加施药剂量和次数也不能有效降低红火蚁蚁巢数量,甚至会引起并加剧蚁巢的分巢扩散,建议在红火蚁防控中应慎重使用,或仅用于局部应急防控工作。 | 齐国君 王刚 石庆型 陆嘉进 关子盈 陈婷 陈洁 Muhammad Irfan Waris 吕利华 | 2023 | 植物保护学报2023，50，5： | 1 |
| 4 | Molecular and in vitro biochemical assessment of chemosensory protein 10 from brown planthopper Nilaparvata lugens at acidic pH显示文摘Chemosensory proteins(CSPs)are important molecular components of the insect olfactory system,which are involved in capturing,binding,and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect behavior.This protein family(CSPs)is also involved in many other systems that are not linked to olfactory receptors in olfactory sensilla.The brown planthopper(BPH)is a monophagous pest of rice that causes damage by sucking phloem sap and transmitting a number of diseases caused by viruses.In this study,fluorescence competitive binding assay and fluorescence quenching assay at acidic p H were performed as well as homology modelling to describe the binding affinity of Nlug CSP10.Fluorescence competitive binding assay(FCBA)demonstrated that Nlug CSP10 bound strongly to nonadecane,farnesene,and 2-tridecanone at acidic p H.The results of FCBA indicated that Nlug CSP10 bound different ligands at the physiological p H(5.0)of the bulk sensillum lymph.Fluorescence quenching assay demonstrated that Nlug CSP10 generated a stable complex with 2-tridecanone,while two ligands nonadecane and farnesene collided due to molecular collisions.The interaction of selected ligands with the modelled structure of Nlug CSP10 was also analyzed,which found the key amino acids(Gln23,Gln24,Gln25,Asn27,Met33,Ser34,Ile35,Tyr36,Asn42,Met43,Val45,Asn46,Asn93,Arg96,Ala97,Lys99,and Ala100)in Nlug CSP10 that were involved in binding of volatile compounds.The present study contributes to the binding profile of Nlug CSP10 that promotes the development of behaviorally active ligands based on BPH olfactory system. | Muhammad Irfan WARIS Aneela YOUNAS Rana Muhammad Kaleem ULLAH Fatima RASOOL Muhammad Muzammal ADEEL WANG Man-qun | 2022 | Journal of Integrative Agriculture2022，21，3： | 0 |
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