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| 1 | 赭曲霉毒素A和玉米赤霉烯酮二联定量胶体金免疫层析试纸条的制备及应用(英文)显示文摘目的:优化建立二联胶体金免疫层析试纸条,实现对玉米、面粉和饲料中赭曲霉毒素A和玉米赤霉烯酮的同时快速定量检测。创新点:采用纳米金颗粒标记真菌毒素单克隆抗体,基于竞争反应模式,通过对金颗粒尺寸、免疫层析相关组成材料及缓冲液配方的系列优化,同时借助手持式信号读取装备,实现对谷物和饲料中赭曲霉毒素A和玉米赤霉烯酮的同时定量检测。该方法简单、快速且成本低,与液相色谱串联质谱(LC-MS/MS)一致性良好,便于基层单位的推广使用。方法:采用柠檬酸钠还原法制备纳米金颗粒并标记获得金标抗体。通过在基于竞争反应的免疫层析检测体系中,同时设置两条检测线(T1、T2)以实现两种毒素的同时检测,最终采用金标信号读数仪实现定量检测。结论:制备的二联定量胶体金试纸条对赭曲霉毒素A和玉米赤霉烯酮的检测限分别为0.32和0.58 ng/ml(图5),在玉米、小麦和饲料样本中的加标回收率为77.3%~106.3%,且变异系数均小于15%(表2)。制备的二联定量胶体金免疫层析试纸条和LC-MS/MS对玉米、小麦和饲料等天然样本中赭曲霉毒素A和玉米赤霉烯酮的定量检测结果相关性较好(表3和图7),具有较好的应用价值。 | Xian ZHANG Ke HE Yun FANG Tong CAO Narayan PAUDYAL Xiao-feng ZHANG Hou-hui SONG Xiao-liang LI Wei-huan FANG | 2018 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2018，19，11： | 7 |
| 2 | 禽肉弯曲菌污染分析及冷藏条件下失活动力学特征显示文摘为了解空肠弯曲菌(Campylobacler jejuni) 和结肠弯曲菌(Campylobacler coli) 在浙江省肉鸡产业链中的污染状况,及其冷藏过程中的失活特征.研究采用多重P C R 方法对肉鸡养殖、屠宰和销售环节中分别采集的肛拭子、屠宰场环境和鸡肉等样品进行检测,平板计数法对人工污染鸡肉进行活菌数检测,用于失活特征分析.结果显示,采集的样品中,C.jejuni和C.coli的检出率分别为4 5 .3 % ( 1 8 2 / 4 0 2 )和4. 9 % ( 2 0 / 4 0 2 ) ; 其中,养殖环节中的检出率分别为81. 2% ( 6 5 / 8 0 ) 和0 , 屠宰环节中的检出率分别为30. 5% ( 4 8 / 1 5 7 ) 和5. 1%( 8 / 1 5 7 ) ,销售环节中的检出率分别为41.8%(69/165) 和7. 3% (12/165) .在6 ℃冷藏条件下,鸡肉中的C.jejuni和C.coli失活曲线具有相似的特征,在30 d 贮藏期内, lg r fu 分别下降了1.98和2.19 ;其中,0-14d 细菌数量下降速度较快, 14 - 30 d 细菌数量下降较为平缓.Log-logistic模型相比于W eibull模型对2 种细菌的失活曲线有较好的拟合性.上述研究表明,肉鸡养殖、屠宰和销售环节中均存在着一定程度的空肠弯曲菌和结肠弯曲菌污染,其中,销售环节中检出率髙于屠宰环节;在6℃冷藏条件下,Log-logistic方程作为2 种菌的一级预测生长模型具有较好的拟合性. | 刘玺 潘航 Narayan Paudyal 方维焕 李肖梁 | 2016 | 浙江农业学报2016，28，11： | 2 |
| 3 | Routine use of antibiotic prophylaxis in low-risk laparoscopic cholecystectomy is unnecessary: A randomized clinical trial显示文摘 | Jay Narayan Shah Shanta Bir Maharjan Sanjay Paudyal | 2012 | Asian Journal of Surgery2012，，4： | 1 |
| 4 | 满膛与净膛冷鲜鸡微生物污染特征分析显示文摘探索满膛与净膛方式屠宰加工的冷鲜鸡中重要微生物污染情况,以期为冷鲜鸡的微生物控制技术开发提供依据。研究分别采集屠宰加工后第0~2天的满膛鸡与净膛鸡的开膛处肌肉,采用国标法和实时荧光定量检测法进行总菌数检测以及大肠菌群、空肠弯曲菌等微生物分类计数,并使用SPSS软件进行统计学分析。研究结果表明,净膛鸡中菌落总数、大肠菌群数和空肠弯曲菌3个指标的检测合格率均低于满膛鸡;净膛鸡的总菌数和大肠菌群数显著高于满膛鸡;不同禽类屠宰场来源的满膛鸡总菌数和大肠菌群数差异显著,而净膛鸡差异不显著;在第0~2天低温(4℃)储存货架期内,净膛鸡的微生物污染变化与满膛鸡差异不显著,但净膛鸡中微生物的增殖速度较满膛鸡缓慢。16S rDNA检测,净膛鸡和满膛鸡中均存在多种微生物,但在种类上也存在着一定的差异,其中阴沟肠杆菌等7种微生物未在满膛鸡中检测到。净膛鸡和满膛鸡微生物均存在着超标现象;净膛鸡中的二次污染较为严重;在货架期保鲜方面,净膛鸡具有优势。结果为冷鲜鸡的微生物控制技术开发以及净膛工艺的优化提供依据。 | 廖夏旖 潘航 Narayan Paudyal 李国炜 刘亚杰 方维焕 李肖梁 | 2017 | 浙江农业科学2017，58，5： | 0 |
| 5 | RETRACTED: Innovative Practices for the Promotion of Local/Indigenous Knowledge for Disaster Risk Reduction Management in Sudur Paschim Province, Nepal显示文摘This article has been retracted.The original article has previously been published elsewhere without disclosure to the editor,permission to republish,or justification(i.e.,redundant publication).We apologise for any inconvenience this retraction may have caused readers.Refers to:RETRACTED:Innovative Practices for the Promotion of Local/Indigenous Knowledge for Disaster Risk Reduction Management in Sudur Paschim Province,Nepal Kabi Prasad Pokhrel,Shambhu Prasad Khatiwada,Narayan Prasad Paudyal,Keshav Raj Dhakal,Chhabi Lal Chidi,Narayan Prasad Timilsena,Dhana Krishna Mahat Journal of Geographical Research,Volume 4,Issue 3,July 2021. | Kabi Prasad Pokhrel Shambhu Prasad Khatiwada Narayan Prasad Paudyal Keshav Raj Dhakal Chhabi Lal Chidi Narayan Prasad Timilsena Dhana Krishna Mahat | 2022 | Journal of Geographical Research2022，5，2： | 0 |
| 6 | Innovative Practices for the Promotion of Local/Indigenous Knowledge for Disaster Risk Reduction Management in Sudur Paschim Province,Nepal显示文摘This study is an attempt to point out the different types of natural hazards in Sudur Paschim province of Nepal where environmental degradation processes such as deforestation,desertification,biodiversity loss,soil crisp and watershed degradation are rising trends.Using participatory method multi hazard prone areas were identified with type and intensity.Multi criteria evaluation method was applied to prioritize replicable actions with location-specific innovative practices and their legitimization for integrating local and indigenous knowledge into mainstream education,science and policy with a view to incorporate local and indigenous knowledge as live science in disaster and climate change education.Findings of the study reveal that varieties of natural hazards in combination with social factors such as poverty,conflict and inequality have resulted frequent disasters and social vulnerabilities in many parts of the province.Traditionally,indigenous and local people have responded threats of multi hazards by using their traditional knowledge and skills which has evolved over generations,and continue to adapt to future changes.These traditional,often faith-based,beliefs and practices were found to use as the key to their resilience in the face of natural hazards.However,many communities have been lost their knowledge as the elderly die without transferring it to younger members.Behind this proper educational policy and strategic development plans have not been implemented to cope local/indigenous knowledge into practices.Findings of the study indicate that indigenous and local knowledge is a precious province resource that can support the process of disaster prevention,preparedness and response in cost-effective disaster risk reduction.Therefore,policy framework has to prime focus to integrate indigenous and local knowledge,wisdom and skills into mainstream educational programs in order to transfer science into policy and education(words)into practice. | Kabi Prasad Pokhrel Shambhu Prasad Khatiwada Narayan Prasad Paudyal Keshav Raj Dhakal Chhabi Lal Chidi Narayan Prasad Timilsena Dhana Krishna Mahat | 2021 | Journal of Geographical Research2021，4，3： | 0 |
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