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| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | Macrophage-tumor cell interactions identification of MUC1 on breast cancer cells as a potential counter-receptor for the macrophage-restricted receptor,sialoadhesin显示文摘 | Hartnell A Happerfield L | 1999 | Immunology1999，98，： | 1 |
| 3 | Cadmium and mercury removal from non-point source wastewatcr by a hybrid bioreactor 显示文摘 | YAN Rong YANG Fan WU Yong-hong HU Zheng-yi NATH B YANG L Z FANG Yah-ruing | 2011 | Bioresource Technology2011，102，99： | 1 |
| 4 | Effect of laser annealing on electrical and optical properties n-mercury cadmium telluride显示文摘 | Dawar A L Savita Roy Tirlok Nath | 1991 | J Appl Phys1991，69，7： | 1 |
| 5 | Spreading of neurodegenerative pathology via neuron-to-neuron trans- mission of beta-amyloid 显示文摘 | Nath S Agholme L Kurudenkandy F R | 2012 | Journal of Neuroscience2012，32，26： | 1 |
| 6 | Productivity improvement ofprecast shop drawings generation through BIM- based process re-engineering显示文摘 | Nath T Attarzadeh M Tiong R L K | 2015 | Automation in Construction2015，，54： | 1 |
| 7 | Detection of 1 ,N^2-propanodeoxyguanosine adduets as potential endogenous DNA lesions in rodent and human tissues 显示文摘 | Nath R G Ocando J Chung F L | 1996 | Cancer Research1996，56，： | 1 |
| 8 | Genome-wide analy- sis of the host intracellular network that regulates surviv- al of mycobacterium tuberculosis 显示文摘 | Kumar D Nath L Kamal MA | 2010 | Cell2010，140，5： | 1 |
| 9 | The sup- ply of corporate social responsibility disclosures among US firms显示文摘 | Holder-Webb L Cohen J R Nath L | 2009 | Journal of Business Ethicsr2009，84，4： | 1 |
| 10 | HPLC-UV assay for monitoring total and unbound mycophenolic acid concentrations in children显示文摘 | Zeng L Nath CE Shaw PJ | 2009 | Biomed Chromatogr2009，23，1： | 1 |
| 11 | Extraction and purification of curtain, pro- teas from the latex of Jatropha curcas Linn 显示文摘 | Nath L K Dutta S K | 1991 | Pharm Pharma- co11991，43，1： | 1 |
| 12 | IN vivo formation of complex microvessela lined by hmnan endothelial ceils in an immunodeficient mouse显示文摘 | Schechner JS Nath AK Zheng L | 2000 | PNAS2000，97，： | 1 |
| 13 | Fluorescence in situ Hybridization (FISH) DNA Probe Production and Hybridization Criteria显示文摘 | Nath J Jogiraler N and Kirby L | 1998 | Biotech Histochem1998，73，： | 1 |
| 14 | Disruption of neurogenesis by amyloid beta-peptide, and perturbed neural progenitor cell homeostasis, in models of Alzheimer' s disease 显示文摘 | Haughey N J Nath A Chan S L | 2002 | J Neurochem2002，83，6： | 1 |
| 15 | Penicillitlm marneffei infection in HIV infected patients in Nagaland&immune reconstitution after Ireatment显示文摘 | Saikia L Nath R Biswanath P et a1 | 2009 | IndianJ Med Res2009，129，3： | 1 |
| 16 | The Supply of CorporateSocial Responsibility Disclosures Among US Firms显示文摘 | Holder Webb L Cohen J R Nath L | 2009 | Journal of Busi ness Ethics2009，84，4： | 1 |
| 17 | In vivo induction of sister chromatid exchanges in mice by nitrosated coal dust extract 显示文摘 | Krishna G Nath J Soler L | 1987 | Environ Res1987，42，1： | 1 |
| 18 | Human immunodeficiency virus type 1 Tat protein directly activates neuronal N-methyl-D-aspartate receptors at an allosteric zinc-sensitive site显示文摘 | Song L Nath A Geiger JD | 2003 | J Neurovirol2003，9，3： | 1 |
| 19 | Analysis of birth intervals in a non-contracepting Indian population: an evolutionary ecological approach 显示文摘 | NATH D C LEONETTI D L STEELE M S | 2000 | Journal of Biosocial Science2000，32，3： | 1 |
| 20 | Dosimetry of interstitial brachytherapy sources:recommendations of AAPM radiation therapy committee task group No43显示文摘 | NATH R ANDERSON L L LUXTON G | 1995 | Medical Physics1995，22，： | 1 |
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