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| 1 | Natural astaxanthin enhanced antioxidant capacity and improved semen quality through the MAPK/Nrf2 pathway in aging layer breeder roosters显示文摘Background:Natural astaxanthin(ASTA)has strong antioxidant properties and has been widely used as a health product to improve human health.However,the effects of ASTA on the reproductive performance of aging roosters have been poorly studied.We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via antioxidation defense system.Methods:In the present study,9653-week-old Jinghong No.1 layer breeder roosters were fed a corn-soybean meal basal diet containing 0,25,50,or 100 mg/kg ASTA for 6 weeks.Results:Semen quality in the ASTA groups remarkably improved than that in the control group,and antioxidant activities,the abilities to scavenge hydroxyl radicals and superoxide anions,increased gradually with ASTA addition(P<0.05).In addition,the mRNA levels of antioxidant enzymes as well as the mRNA and protein levels of the mitogen-activated protein kinase(MAPK)and nuclear factor-erythroid 2-related factor 2(Nrf2)were markedly increased in the 50-100 mg/kg ASTA group(P<0.05).Conclusions:Collectively,these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities and the ability to scavenge hydroxyl radicals,which may be related to upregulation of the MAPK/Nrf2 pathway. | Shan Gao Nuo Heng Fang Liu Yong Guo Yu Chen Liang Wang Hemin Ni Xihui Sheng Xiangguo Wang Kai Xing Longfei Xiao Xiaolong Qi | 2022 | Journal of Animal Science and Biotechnology2022，13，2： | 2 |
| 2 | Sirtuin 3 regulation:a target to alleviateβ-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells显示文摘Background During the transition period,the insufficient dry matter intake and a sharply increased in energy consumption to produce large quantities of milk,high yielding cows would enter a negative energy balance(NEB)that causes an increase in ketone bodies(KBs)and decrease in reproduction efficiency.The excess concentrations of circulating KBs,represented byβ-hydroxybutyric acid(BHBA),could lead to oxidative damage,which potentially cause injury to follicular granulosa cells(fGCs)and delayed follicular development.Sirtuin 3(Sirt3)regulates mitochondria reactive oxygen species(mitoROS)homeostasis in a beneficial manner;however,the molecular mechanisms underlying its involvement in the BHBA-induced injury of fGCs is poorly understood.The aim of this study was to explore the protection effects and underlying mechanisms of Sirt3 against BHBA overload-induced damage of fGCs.Results Our findings demonstrated that 2.4 mmol/L of BHBA stress increased the levels of mitoROS in bovine fGCs.Further investigations identified the subsequent mitochondrial dysfunction,including an increased abnormal rate of mitochondrial architecture,mitochondrial permeability transition pore(MPTP)opening,reductions in mitochondrial membrane potential(MMP)and Ca^(2+)release;these dysfunctions then triggered the caspase cascade reaction of apoptosis in fGCs.Notably,the overexpression of Sirt3 prior to treatment enhanced mitochondrial autophagy by increasing the expression levels of Beclin-1,thus preventing BHBA-induced mitochondrial oxidative stress and mitochondrial dysfunction in fGCs.Furthermore,our data suggested that the AMPK-mTOR-Beclin-1 pathway may be involved in the protective mechanism of Sirt3 against cellular injury triggered by BHBA stimulation.Conclusions These findings indicate that Sirt3 protects fGCs from BHBA-triggered injury by enhancing autophagy,attenuating oxidative stress and mitochondrial damage.This study provides new strategies to mitigate the fGCs injury caused by excessive BHBA stress in dairy cows with ketosis. | Shanjiang Zhao Jianfei Gong Yi Wang Nuo Heng Huan Wang Zhihui Hu Haoyu Wang Haobo Zhang Huabin Zhu | 2023 | Journal of Animal Science and Biotechnology2023，14，4： | 1 |
| 3 | Human umbilical cord mesenchymal stromal cells promote the regeneration of severe endometrial damage in a rat model显示文摘Due to the influence of various social and environmental factors,the incidence of infertility is increasing,and the application of assisted reproductive technology(ART)is becoming more and more widespread.At present,the clinical pregnancy rate of in vitro fertilization(IVF)is 40%~60%,and decreased endometrial receptivity is one of the reasons for implantation failure.Endometrial receptivity refers to the capacity of the endometrium to accept embryos.Intrauterine adhesions(IUA),also known as Asherman syndrome(AS). | Mei Zhuang Wuwen Zhang Nuo Cheng Ling Zhou Dan Liu Hua Yan Ge Fang Boon Chin Heng Yan Sun Guoqing Tong | 2022 | Acta Biochimica et Biophysica Sinica2022，54，1： | 1 |
| 4 | A Better Instrument for Screening Diabetes in Rural Areas of China: An Equation Developed from Multivariate Logistic Regression or a Simplified Scoring Form显示文摘Diabetes has become a major public health concern in the world and the total number of diabetic patients is considered to soar to 366 million in 2030 [1] . Emerging evidence has suggested that earlier detection of type 2 diabetes, glycaemic control improvement and intensified risk factor | ZHAO Xiao Long HE Xiao Ye ZHANG Heng Sheng LU Bin LI Jin Hui LI Yi Ming ZHOU Li Nuo HU Ren Ming | 2013 | Biomedical and Environmental Sciences2013，26，6： | 0 |
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