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123篇 您的检索式：作者名="PRATHER R S"

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	题名	作者	年代	出处	被引量
1	Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research.	LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER	2008	Chinese Science Bulletin2008，53，7：	8
2	Nucleartransplantation in the bovine embryo: as-sessmentof donor nuclei and recipient oocyte显示文摘	Prather R S	1987	Biol Reprod1987，37，：	1
3	CAB reviews : perspectives in agriculture, veterinary science 显示文摘	Ross J W Prather R S Whyte J J	2009	Nutr Nat Re- sour2009，4，38：	1
4	Strategies for activating nu- clear transfer oocytes显示文摘	Machaty Z Prather R S	1998	Rep Rod Fertil Dev1998，10，78：	1
5	Cloning embryos by nuclear transfer显示文摘	Prather R S Barnes F L	1990	Reprod Fertil1990，41，：	1
6	Artificial activation of porcine oocytes matured in vitro显示文摘	PRATHER R S EICHEN P A NICKS D K	1991	Molecular Reproduction Development1991，28，：	1
7	Pigs is pigs显示文摘	Prather R S	2000	Science2000，289，：	1
8	Differential effects of protein synthesis inhibitors on porcine oocyte activation显示文摘	Nussbaum D J Prather R S	1995	Molecular Reproduction Development1995，41，：	1
9	Strategies for activation nucle- ar transfer oocytes 显示文摘	MACHATY Z PRATHER R S	1998	Reproduction Fertility and Develop- ment1998，10，：	1
10	Strategies for activating nuclear transfer oocytes显示文摘	MACHATY Z PRATHER R S	1998	Reprod Fertil Dev1998，10，：	1
11	Response of porcine oocytes to electrical and chemical activation during maturation in vitro显示文摘	HAGEN D R PRATHER R S FIRST N L	1991	Mol Reprod Develop1991，28，：	1
12	Production of cloned pigs by using somatic cells as donors显示文摘	Lai L X Prather R S	2003	Cloning and Stem Cells2003，5，4：	1
13	Production of cloned pigs by using somatic cells as donors显示文摘	Lai L Prather R S	2003	Cloning and Stem Cells2003，5，4：	1
14	Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation显示文摘	Kim N H Funahashi H Prather R S	1996	Mol Reprod Dev1996，43，2：	1
15	Development of pig embryos reconstructed by microinjection of cultured fetal fibroblast cells into in vitro matured oocytes显示文摘	Tao T Machaty Z Boquest A C Day B N Prather R S	1999	Anim Reprod Sci1999，56，2：	1
16	Maturation in vitro of pig oocytes in protein-free culture media: fertilization and subsequent embryo development in vitro显示文摘	ABEYDEERA L R WANG W H PRATHER R S	1998	Biol Reprod1998，58，：	1
17	Creating genetically modified pigs by using nuclear transfer显示文摘	LAI L PRATHER R S	2003	Reprod Biol Elldoerino2003，1，：	1
18	Culture of porcine embryos from the one and two-cell stage to the blastocyst stage in sheep oviducts 显示文摘	PRATHER R S SIMS M M FIRST N L	1991	Thenogenology1991，35，：	1
19	Classification, lithologic calibration and stratigraphic succession of seismics facies from introslope basins, deep water Gulf of Mexico, USA显示文摘	PRATHER B E BOOTH J R STEFFENS G S	1998	AAPG Bulletin1998，82，5：	1
20	Feasibility of producing porcine nuclear transfer embryos by using G2/M-stage fetal fibroblasts as donors显示文摘	Lai L Tao T Machaty Z Kuhholzer B Sun Q Y Park K W Day B N Prather R S		0，，：	1

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