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您的检索式：作者名="Pedro Laborda"
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| 1 | Effects of Rosmarinus officinalis and Salvia officinalis essential oils on Tetranychus urticae Koch (Acari: Tetranychidae)显示文摘 | Rafael Laborda Israel Manzano Miguel Gamón Isabel Gavidia Pedro Pérez-Bermúdez Rafael Boluda | 2013 | Industrial Crops & Products2013，，： | 1 |
| 2 | Evaluation of suitable reference genes for normalization of quantitative real-time PCR analysis in rice plants under Xanthomonas oryzae pv.oryzae--infection and melatonin supplementation显示文摘Exogenous melatonin(MT)was found to be an interesting tool for enhancing the resistance of rice to Xanthomonasoryzaepv.oryzae(Xoo)-caused bacterial blight(BB).However,the accurate comparison of the expression levels across samples was a challenging task.In this work,the stability of 10 common used housekeeping genes under Xoo-infection and MT supplementation in rice was analyzed using quantitative real-time PCR(qRT-PCR),and algorithms geNorm,NormFinder and BestKeeper.Our results indicated that most reference genes remained stable in Xoo-infected rice plants,while a number of reference genes were affected by MT supplementation.Among all studied genes,the transcript levels of 18S(18S ribosomal RNA)and UBC(Ubiquitin-conjugating enzyme E2)remained unaltered by Xoo infection,while UBC and UBQ5(Ubiquitin 5)were the most stable genes when examining simultaneous Xoo-infection and MT supplementation,demonstrating that UBC is a suitable reference gene for qRT-PCR data normalization in rice under Xoo-infection and MT supplementation. | Xian Chen Pedro Laborda Yan Dong Fengquan Liu | 2020 | Food Production, Processing and Nutrition2020，2，1： | 0 |
| 3 | Novel chemical-and protein-mediated methods for glucosamine detection显示文摘We describe two novel approaches for the determination of glucosamine(GlcN).The first approach is based on the chemical derivatization of GlcN with the non-fluorophor 1,3-diphenyl-1,3-propanedione(DPPD),which results in a condensation product with interesting fluorescent properties.The obtained compound was isolated by silica-gel chromatography and its structure elucidated by NMR and mass spectrometry.The second approach is based on a previously undescribed sensitivity of the enzyme glucosamine-6-phosphate deaminase(GPDA)towards GlcN,which resulted in the precipitation of the enzyme.Using a rational enzyme engineering approach and both liquid-based and plate-based screening methods,mutational GPDA variants with significantly improved precipitation properties were identified and characterized.These novel glucosamine detection methods may be a useful addition to the repertoire of currently available glucosamine detection sensors. | Linshu Chen Pedro Laborda Zhipeng Cai Andrew Kevin Hagan Aimin Lu Josef Voglmeir Li Liu | 2022 | Food Materials Research2022，2，1： | 0 |
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