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2篇 您的检索式:作者名="Pierre Comizzoli"
    题名 作者 年代 出处 被引量
1Biobanking efforts and new advances in male Fertility preservation for rare and endangered species显示文摘理解并且支撑的生物多样性是高度得益于创造的一门多学科的科学组织并且 biomaterials (染色体资源银行) 的可存取的集合。大 cryo 收集是为理解的无价的工具,编目,并且保护世界的唯一的动物和植物的基因差异。明确地,从稀罕种类的精液的系统的收集和保藏与某 biobanks 现在活跃地为危及的种类管理和繁殖正在被使用在最近的十年显著地被开发了(包括象黑脚的细带和巨大的熊猫那样的种类的介绍) 。为人,家畜种类,和实验室动物从男富饶保藏的成长的地出现的革新也与保护和珍贵国内、野的种类的繁殖正在变得相关。这些新途径在 “ 以外延长; classical”与精子结冰包括阴囊的织物保藏联系的方法与 xenografting 或在 vitro 文化结合了,所有哪个为 rescuing 有潜力闲置的 germplasm 的广阔数量。也在被预言在下一十年(干细胞技术,在周围的温度的精子房间的简历稳定,和 genomics 工具的使用) 以内有高影响的开发中有另外的选择。然而, biobanking 努力和新富饶保藏策略不得不在哺乳动物的种类以外扩展方法,它将提供知识和工具更好管理种类担任珍贵生物医学的模型或要求帮助颠倒危及。Pierre Comizzoli 2015Asian Journal of Andrology2015,17,4:1
2Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells显示文摘Human induced pluripotent stem cells(hiPSCs)possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use.However,contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum.The latter may cause spontaneous differentiation and/or introduce xenogeneic factors,which may compromise the quality of hiPSCs.Here,sand from nature is discovered to be capable of seeding ice above10◦C,which enables cryopreservation of hiPSCs with no serum,much-reduced cryoprotectant,and high cell survival.Furthermore,the cryopreserved hiPSCs retain high pluripotency and functions judged by their pluripotency marker expression,cell cycle analysis,and capability of differentiation into the three germ layers.This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine.Bin Jiang Weijie Li Samantha Stewart Wenquan Ou Baolin Liu Pierre Comizzoli Xiaoming He 2021Bioactive Materials2021,6,12:1
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