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| 1 | Genome-wide MicroRNA Expression Profiles in COPD: Early Predictors for Cancer Development显示文摘Chronic obstructive pulmonary disease(COPD) significantly increases the risk of developing cancer. Biomarker studies frequently follow a case-control set-up in which patients diagnosed with a disease are compared to controls. Longitudinal cohort studies such as the COPD-centered German COPD and SYstemic consequences-COmorbidities NETwork(COSYCONET) study provide the patient and biomaterial base for discovering predictive molecular markers. We asked whether microRNA(miRNA) profiles in blood collected from COPD patients prior to a tumor diagnosis could support an early diagnosis of tumor development independent of the tumor type. From 2741 participants of COSYCONET diagnosed with COPD, we selected 534 individuals including 33 patients who developed cancer during the follow-up period of 54 months and 501 patients who did not develop cancer, but had similar age, gender and smoking history. Genome-wide miRNA profiles were generated and evaluated using machine learning techniques. For patients developing cancer we identified nine miRNAs with significantly decreased abundance(two-tailed unpaired t-test adjusted for multiple testing P < 0.05), including members of the miR-320 family. The identified mi RNAs regulate different cancer-related pathways including the MAPK pathway(P = 2.3 x 10^(-5)). We also observed the impact of confounding factors on the generated miRNA profiles, underlining the value of our matched analysis. For selected miRNAs, qRT-PCR analysis was applied to validate the results. In conclusion, we identified several miRNAs in blood of COPD patients, which could serve as candidates for biomarkers to help identify COPD patients at risk of developing cancer. | Andreas Keller Tobias Fehlmann Nicole Ludwig Mustafa Kahraman Thomas Laufer Christina Backes Claus Vogelmeier Caroline Diener Frank Biertz Christian Herr Rudolf A. Jorres Hans-Peter Lenhof Eckart Meese Robert Bals | 2018 | Genomics, Proteomics & Bioinformatics2018，16，3： | 9 |
| 2 | Proximal points are on the fast track显示文摘 | Robert Mifflin Claudia Sagastizábal | 2002 | Journal of Convex Analysis2002，9，2： | 1 |
| 3 | Antimicrobiai Peptide Current Status and Therapeutic Potential Andreas显示文摘 | Koczulla Andreas R Bals Robert | 2003 | Drugs2003，63，4： | 1 |
| 4 | The interleukin-6 (?174) G/C promoter polymorphism is associated with type-2 diabetes mellitus in Native Americans and Caucasians显示文摘 | Barbora Vozarova José-Manuel Fernández-Real William C. Knowler Lluis Gallart Robert L. Hanson Jonathan D. Gruber Wilfredo Ricart Joan Vendrell Cristóbal Richart P. Antonio Tataranni Johanna K. Wolford | 2003 | Human Genetics2003，，4： | 1 |
| 5 | Mouse β-Defensin 1 Is a saltsensitive antimicrobial peptide present in epithelia of the lung and urogenital tract显示文摘 | Mitchell J | 1998 | Infect Immun1998，66，3： | 1 |
| 6 | Thrombospondin-1 as a Biomarker in Pulmonary Hypertension显示文摘 | Kaiser Ralf H Frantz Christian Bals Robert | | 0，，4： | 1 |
| 7 | Epithelial antimicrobial peptides in host defense against in2 feetion 显示文摘 | Robert Bals | 2000 | Respir Research2000，1，3： | 1 |
| 8 | Antimicrobial peptides:Current status and therapeutic otential显示文摘 | Robert Bals | 2003 | Drugs2003，63，： | 1 |
| 9 | Identification of a novel, multifunctional β-defensin (human β-defensin 3) with specific antimicrobial activity显示文摘 | José-Ramón García Florian Jaumann Sandra Schulz Alexander Krause Javier Rodríguez-Jiménez Ulf Forssmann Knut Adermann Enno Klüver Claus Vogelmeier Dirk Becker Rainer Hedrich Wolf-Georg Forssmann Robert Bals | 2001 | Cell and Tissue Research2001，，2： | 1 |
| 10 | Impact of Retinopathy of Prematurity Screening Examination on Cardiorespiratory Indices显示文摘 | Achyut N. Mukherjee Patrick Watts Hasan Al-Madfai Bal Manoj Dawn Roberts | 2006 | Ophthalmology2006，，9： | 1 |
| 11 | Antimicrobial peptides:Current status and therapeutic potential显示文摘 | ANDREAS R ROBERT Bals | 2003 | Drugs2003，63，4： | 1 |
| 12 | Mouse β-defensin 1 is a salt-sensitive antimicrobial peptide present in epithelia of the lung and urogenital tract显示文摘 | Mitchell J | 1998 | Infect Immun1998，66，3： | 1 |
| 13 | Epithelial antimicrobial peptides in host defense against infection显示文摘 | Robert Bals | 2000 | Respir Res2000，1，3： | 1 |
| 14 | Antimicrobial peptides: current status and therapeutic potential显示文摘 | Andreas R Koczulla Robert Bals | 2003 | Drugs2003，63，4： | 1 |
| 15 | Surface modifica- tion of nitrile rubber by plasma polymerization显示文摘 | ROBERT J RATWAY CMAUR ICE BAL IK | 1998 | Plasmas and Polymers1998，3，2： | 1 |
| 16 | Mouse β-defensin-1 is a saltsensitive antimicrobial peptide present in epithelia of the lung and urogenital tract显示文摘 | ROBERT Bals MITCHELL J | 1998 | Infect Immun1998，66，3： | 1 |
| 17 | Surgical outcomes and safrty of femtosecond laser cataract surgery:a prospective study of 1500 consecutive cases 显示文摘 | Roberts TV Lawless M Bal Si | 2013 | Ophthalmology2013，120，： | 1 |
| 18 | Identification of a novel, multifunctional β-defensin (human β-defensin 3) with specific antimicrobial activity显示文摘 | José-Ramón García Florian Jaumann Sandra Schulz Alexander Krause Javier Rodríguez-Jiménez Ulf Forssmann Knut Adermann Enno Klüver Claus Vogelmeier Dirk Becker Rainer Hedrich Wolf-Georg Forssmann Robert Bals | 2001 | Cell and Tissue Research2001，，2： | 1 |
| 19 | Systematic Cross-biospecimen Evaluation of DNA Extraction Kits for Long-and Short-read Multi-metagenomic Sequencing Studies显示文摘High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases.We thus collected bile,stool,saliva,plaque,sputum,and conjunctival swab samples and performed DNA extraction with three commercial kits.For each combination of the specimen type and DNA extraction kit,20-gigabase(Gb)metagenomic data were generated using short-read sequencing.While profiles of the specimen types showed close proximity to each other,we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits.No kit outperformed all selected kits on every specimen.We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit.Depending on the specimen,our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution,but DNA-based identification is superior to identification by mass spectrometry.Finally,longread nanopore sequencing confirmed the results(correlation coefficient>0.98).Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients. | Jacqueline Rehner Georges Pierre Schmartz Laura Groeger Jan Dastbaz Nicole Ludwig Matthias Hannig Stefan Rupf Berthold Seitz Elias Flockerzi Tim Berger Matthias Christian Reichert Marcin Krawczyk Eckart Meese Christian Herr Robert Bals Soren L.Becker Andreas Keller Rolf Muller The IMAGINE Consortium | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 0 |
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