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| 1 | Suppressing tawny crazy ant (Nylanderia fulva) by RNAi technology显示文摘A bstract The tawny crazy ant(Nylanderia fulva)is a new invasive pest in the United States.At present,its management mainly relies on the use of synthetic insecticides,which are generally ineffective at producing lasting control of the pest,necessitating alternative environmentally friendly measures.In this study,we evaluated the feasibility of gene silencing to control this ant species.Six housekeeping genes encoding actin(NfActin),coatomer subunit β (NfCOPP),arginine kinase(NfArgK),and V-type proton ATPase subunits A(NfvATPaseA),B(NfvATPaseB)and E(NfvATPaseE)were cloned.Phylogenetic analysis revealed high sequence similarity to homologs from other ant species,particularly the Florida carpenter ant(Camponotus floridanus).To silence these genes,vector L4440 was used to generate six specific RNAi constructs for bacterial expression.Heat-inactivated,dsRNA-expressing Escherichia coli were incorporated into artificial diet.Worker ants exhibited reduced endogenous gene expression after feeding on such diet for 9 d.However,only ingestion of dsRNAs of NfCOPfi(a gene involved in protein trafficking)and NfArgK(a cellular energy reserve regulatory gene in invertebrates)caused modest but significantly higher ant mortality than the control.These results suggest that bacterially expressed dsRNA can be orally delivered to ant cells as a mean to target its vulnerabilities.Improved efficacy is necessary for the RNAi-based approach to be useful in tawny crazy ant management. | Jia Meng Jiaxin Lei Andrew Davitt Jocelyn RHolt Jian Huang Roger Gold Edward L Vargo Aaron MTarone Keyan Zhu-Salzman | 2020 | Insect Science2020，27，1： | 2 |
| 2 | Ionic liquid - in - oil microemulsions显示文摘 | EASTOE J GOLD S ROGERS S E | 2005 | J Am Chem Soc2005，127，20： | 1 |
| 3 | Monozygotic twins discordant for trisomy 21显示文摘 | Voullaire L Gold H | 1982 | Am J Med Genet1982，11，2： | 1 |
| 4 | Does breast feeding protect against non-gastric infections显示文摘 | Golding J Emmett PM Rogers IS | 1997 | Early Hum Dev1997，，： | 1 |
| 5 | Ionic liquid-in-oil microemulsions 显示文摘 | Eastoe J Gold S Rogers S E | 2005 | Journal of the American Chemical Society2005，127，20： | 1 |
| 6 | Studying sex and gender differences in pain and analgesia: A consensus report显示文摘 | Joel D. Greenspan Rebecca M. Craft Linda LeResche Lars Arendt-Nielsen Karen J. Berkley Roger B. Fillingim Michael S. Gold Anita Holdcroft Stefan Lautenbacher Emeran A. Mayer Jeffrey S. Mogil Anne Z. Murphy Richard J. Traub | 2007 | Pain2007，，： | 1 |
| 7 | Monozygotic twins discordant for trisomy 21显示文摘 | Rogers JG Voullaire L Gold H | 1982 | Am J Med Genet1982，11，2： | 1 |
| 8 | Ionic liquid-in-oil microemul-sions显示文摘 | Eastoe J Gold S Roger SE | | 0，，20： | 1 |
| 9 | Designed CO2-philes stabilize water-in- carbon dioxide mieroemulsions 显示文摘 | Eastoe J Gold S Rogers S | 2006 | Angewandte Chemic International Edition2006，45，22： | 1 |
| 10 | Ionic liquid-in-oil microemulsions显示文摘 | Eastoe J Gold S Rogers S E | 2005 | J Am Chem Soc2005，127，： | 1 |
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