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| 1 | hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis显示文摘AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by ANOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric carcinogenesis.RESULTS: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RNA(mi RNA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMNT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal metaplasia samples and down-regulated in normal gastric mucosa and intestinal-type gastric adenocarcinoma samples. Non-atrophic chronic gastritis and intestinal metaplasia are significantly different from normal gastric mucosa samples. hsa-mi R-135 b expression presented a greater discriminatory accuracy between normal samples and gastric lesions. This mi RNA was associated with Helicobacter pylori presence in non-atrophic chronic gastritis samples and regulates the APC and KLF4 tumour suppressor genes.CONCLUSION: Our results provide evidence of epigenetic alterations in non-atrophic chronic gastritis and intestinal metaplasia and suggest that hsa-mi R-29 c and hsa-mi R-135 b are promising biomarkers of gastric carcinogenesis. | Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi Nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos | 2016 | World Journal of Gastroenterology2016，22，6： | 7 |
| 2 | Abnormal pharyngoesophageal function ininfants and young children : Diagnosis with highresolution mano-metry显示文摘 | Rommel N Omari T | 2011 | J Pediatr Gastroenterol Nutr2011，52，1： | 1 |
| 3 | Rumination or belching- regurgitation? Differential diagnosis using oesophageal impedance-manometry显示文摘 | Rommel N Tack J Arts J | 2010 | Neurogastroenterol Motil2010，22，4： | 1 |
| 4 | Baclofen improves symptoms and reduces postprandial flow events in patients with rumination and supragastric belching显示文摘 | Blondeau K Boecxstaens V Rommel N | 2012 | Gastroenterol Hepatol2012，10，4： | 1 |
| 5 | The complexity of feeding problems in 700 infants and young children presenting to a tertiary care institution显示文摘 | Rommel N De Meyer AM Feenstra L | 2003 | Pediatr Gastroenterol Nutr2003，37，1： | 1 |
| 6 | Autosomal dominant isolated velopharyngeal insufficiency显示文摘 | Vantrappen G Rommel N Wellens W | 2002 | Clin Genet2002，1，： | 1 |
| 7 | Performance evaluation of the 'large SMADES' autonomous desalination solar-driven membrane distillation plant in Aqaba, Jordan 显示文摘 | Banat F Jwaied N Rommel M | 2007 | Desalination2007，217，13： | 1 |
| 8 | Efficient single glazed flat plate photovoltaic–thermal hybrid collector for domestic hot water system显示文摘 | Patrick Dupeyrat Christophe Ménézo Matthias Rommel Hans-Martin Henning | 2011 | Solar Energy2011，，7： | 1 |
| 9 | Desalination by a 'compact SMADES' autonomous solar powered membrane distillation unit 显示文摘 | Banat F Jwaied N Rommel M Koschikowski J Wieghuas M | 2007 | Desalination2007，217，： | 1 |
| 10 | Performance evaluation of the 'large SMADES' autonomous desalination solar-driven membrane distillation plant in Aqaba, Jordan 显示文摘 | Banat F Jwaied N Rommel M Koschikowski J Wieghaus M | 2007 | Desalination2007，217，： | 1 |
| 11 | Desalination by a 'compact SMADES' autonomous solar powered menabmne distillation unit显示文摘 | Banat F Jwaied N Rommel M | 2007 | Desalination2007，217，13： | 1 |
| 12 | The complexity of feeding problems in 700 infants and young children presenting to a tertiary care institution显示文摘 | Rommel N De Meyer AM Feenstra L | 2003 | J Pediatr Gastroenterol Nutr2003，37，1： | 1 |
| 13 | The complexity of feeding problems in 700 infants and young children presenting to a tertiary care institutions显示文摘 | Rommel N De Meyer AM Feenstra L | | 0，，01： | 1 |
| 14 | Association of COX2 gene hypomethylation with intestinal type gastric cancer in samples of patients from northern Brazil显示文摘 | Cynthia Farias Vieira Melo Carolina Oliveira Gigek Juarez Nóbrega Silva Marilia de Arruda Cardoso Smith Rubistenia Miranda Araújo Rommel Rodríguez Burbano Eleonidas Moura Lima | 2014 | Tumor Biology2014，，2： | 1 |
| 15 | Feeding difficulties in children with food protein induced gastrointestinal allergies显示文摘 | Meyer R Rommel N Oudenhove L | 2014 | J Gastroenterol Hepatol2014，29，10： | 1 |
| 16 | Upper esophageal sphincter compliance and 'somatization'are independently associated with symptom levels in globus patients 显示文摘 | Rommel N | 2011 | Gastroenterology2011，140，1： | 1 |
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