|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | A VPE-like protease NtTPE8 exclusively expresses in the integumentary tapetum and is involved in seed development显示文摘Programmed cell death(PCD)is an essential process for development,and shows conserved cytological features in both plants and animals.Caspases are well-known critical components of the PCD machinery in animals.However,currently few typical counterparts have been identified in plants and only several caspase-like proteases are known to be involved in plant PCD,indicating the existence of great challenge for confirming new caspase-like proteases and elucidating the mechanisms regulating plant PCD.Here,we report a novel cysteine protease,NtTPE8,which was extracted from tobacco seeds and confirmed as a new caspase-like protease.Recombinant NtTPE8 exhibited legumain and caspase-like proteolytic activities,both of which could be inhibited by the pan-caspase inhibitor(Z-VAD-FMK).Notably,NtTPE8 possessed several caspase activities and the capacity to cleave the cathepsin H substrate FVR,indicating a unique character of NtTPE8.NtTPE8 was exclusively expressed in the integumentary tapetum and thus,is the first specific molecular marker reported to date for this cell type.Downregulation of NtTPE8 caused seed abortion,via disturbing early embryogenesis,indicating its critical role in embryogenesis and seed development.In conclusion,we identified a novel caspase-like cysteine protease,NtTPE8,exclusively expressed in the integumentary tapetum that is involved in seed development. | Wei Wang Hanxian Xiong Rongxin Lin Nantian Zhao Peng Zhao Meng-Xiang Sun | 2019 | Journal of Integrative Plant Biology2019，61，5： | 3 |
| 3 | Malaria Surveillance of Entry People During the COVID-19 Epidemic—Guangdong Province,China,October 2020–May 2021显示文摘Summary What is already known about this topic?Malaria control was affected by the coronavirus disease 2019(COVID-19)pandemic.This study conducted active case finding for key flights and key populations to determine malaria transmission.What is added by this report?Surveillance for malaria was conducted for entry personnel coming from areas affected by malaria.It is estimated that at least 100,000 tests were conducted in Guangdong Province. | De Wu Zhuohui Deng Rongxin Lin Qiang Mao Wenchen Lu Caiwen Ruan Yongzhen Cen Ning Xiao Tie Song | 2021 | China CDC weekly2021，3，38： | 2 |
| 4 | Safety and efficacy of a modified XELOX adjuvant regimen for patients with operated stage III colon cancer:a Chinese single-center experience显示文摘Background:A fixed 8-cycle oxaliplatin and capecitabine(XELOX)regimen has been the standard adjuvant therapy for patients with stage III colon cancer.However,completing the full-cycle of oxaliplatin is often associated with severe neurotoxicity.To spare patients from the toxic effects,without comprising the required efficacy,we evaluated the safety and efficacy of a modified XELOX(mXELOX)adjuvant chemotherapy regimen with 6 cycles of oxaliplatin and a full cycle of capecitabine.Methods:We retrospectively analyzed 330 eligible patients with stage III colon cancer who underwent cura-tive tumor resection followed by mXELOX,standard XELOX or unfinished XELOX adjuvant chemotherapy between December 2007 and April 2015.Associated prognostic factors were investigated and their disease-free survival(DFS)and overall survival(OS)rates were also determined and compared among the different regimen groups.Results:Compared with the standard XELOX group,the mXELOX group had lower total incidence rates of neuro-toxicity(39.3%vs.76.2%,P<0.001),leucopenia(53.6%vs.69.8%,P=0.017)and thrombocytopenia(38.1%vs.56.3%,P=0.011).The standard XELOX and mXELOX adjuvant chemotherapy regimens presented with comparable 3-year DFS rates(86.3%vs.89.2%;P=0.838)and 3-year OS rates(92.7%vs.97.6%;P=0.227).Compared to unfinished XELOX chemotherapy,the oncologic benefits of the mXELOX regimen were greater for patients with T4 tumors(3-year DFS:Hazard ratio[HR],2.184;95%confidence interval[CI],1.051-4.540;P=0.036;3-year OS:HR,4.529;95%CI 1.245-16.479;P=0.022)and for high-risk patients(3-year DFS:HR,1.962;95%CI 0.964-3.993;P=0.044;3-year OS:HR,4.193;95%CI 1.182-14.874;P=0.026).Conclusions:The mXELOX adjuvant chemotherapy presented a comparable survival benefit and lower incidence of toxicity than standard XELOX chemotherapy.It could be an alternative treatment for high-risk patients with operated stage III colon cancer. | Jianhong Peng Weihao Li Rongxin Zhang Junzhong Lin Jinghua Tang Yongshan Wen Zhenhai Lu Xiaojun Wu Zhizhong Pan | 2019 | Cancer Communications2019，39，1： | 2 |
| 5 | Pi:a practical incen- tive protocol for delay tolerant networks显示文摘 | Lu Rongxin Lin Xiaodong Zhu Haojin | 2010 | IEEE Trans on Wire- less Communications2010，9，4： | 1 |
| 6 | Ring-VCO-based phase-locked loops for clock generation–design considerations and state-of-the-art显示文摘This article overviews the design considerations and state-of-the-art of the ring voltage-controlled oscillator(VCO)-based phase-locked loops(PLLs)for clock generation in different applications.Partic-ularly,the objective of the current work is to evaluate the required PLL performance among the fundamental metrics of power,jitter and area.An in-depth treatment of the mainstream PLL architectures and the associated design techniques enables them to be compared analyt-ically and benchmarked with respect to their figure-of-merit(FoM).The paper also summarizes the key concerns on the selection of dif-ferent circuit techniques to optimize the clock performance under dif-ferent scenarios. | Shiheng Yang Jun Yin Yueduo Liu Zihao Zhu Rongxin Bao Jiahui Lin Haoran Li Qiang Li Pui-In Mak Rui P.Martins | 2023 | Chip2023，2，2： | 0 |
| 7 | Original askiatic imaging used in Chinese medicine eye-feature diagnosis of visceral diseases显示文摘Eye-feature diagnosis is a time-homored met hod for studying many diseases in tradit ional Chinese medicine.There is a dlose relationship between eye feature and viscera,and eye feature is a reflect ion of viscer al health status.Commercially used ophthalmology diagnosis instr uments have disadvantages and cannot satisfy the requirements of eye feature diagnosis.In this paper,we proposed a novel askiatic imaging method that removes the interference of an ilumination source's reflection shadow and is free from image splicing.We developed a novel imaging system to implement this method,and some eye feature characteristics to analyze visceral diseases were obtained. | Ning Xue Kai Jiang Qi Li Lili Zhang Li Ma Ruliang Wang Rongxin Fu Xue Lin Ya Su Xiangyu Jin Rongzan Lin Guoliang Huang | 2018 | Journal of Innovative Optical Health Sciences2018，，4： | 0 |
| 8 | Recommendation on data collection and annotation of ocular appearance images in ptosis显示文摘Ptosis is a common ophthalmologic condition,and the diagnosis is primarily based on ocular appearance.Thediagnosis of such conditions can be improved using emerging technology such as artificial intelligence-basedmethods.However,unified data collection and labeling standards have not yet been established.This directlyimpacts the accuracy of ptosis diagnosis based on appearance alone.Therefore,in the present study,we aimedto establish a procedure to obtain and label images to devise a recommendation system for optimal recognitionof ptosis based on ocular appearances.This would help to standardize and facilitate data sharing and serve as aguideline for the development and improvisation of algorithms in artificial intelligence for ptosis. | Jie Meng Binying Lin Dongmei Li Shiqi Hui Xuanwei Liang Xianchai Lin Zhen Mao Xingyi Li Zuohong Li Rongxin Chen Yahan Yang Ruiyang Li Anqi Yan Haotian Lin Danping Huang Chinese Association of Artificial Intelligence,Medical Artificial IntelligenceBranch of Guangdong Medical Association | 2023 | Intelligent Medicine2023，3，4： | 0 |
| 9 | Spin injection into heavily-doped n-GaN via Schottky barrier显示文摘Spin injection and detection in bulk GaN were investigated by performing magnetotransport measurements at low temperatures.A non-local four-terminal lateral spin valve device was fabricated with Co/GaN Schottky contacts.The spin injection efficiency of 21%was achieved at 1.7 K.It was confirmed that the thin Schottky barrier formed between the heavily ndoped GaN and Co was conducive to the direct spin tunneling,by reducing the spin scattering relaxation through the interface states. | Zhenhao Sun Ning Tang Shuaiyu Chen Fan Zhang Haoran Fan Shixiong Zhang Rongxin Wang Xi Lin Jianping Liu Weikun Ge Bo Shen | 2023 | Journal of Semiconductors2023，44，8： | 0 |
| 10 | Injectable self-healing nanocellulose hydrogels crosslinked by aluminum:Cellulose nanocrystals vs.cellulose nanofibrils显示文摘With excellent biocompatibility and unique physiochemical properties,nanocelluloses including cellulose nanocrystals(CNCs)and cellulose nanofibrils(CNFs)are promising candidates for preparing biomedical hydrogels.CNCs and CNFs are different in morphology and surface charges.Herein,CNCs and two CNFs(CNFs-C,Carboxylated CNFs;CNFs-P,Phosphorylated CNFs)were synthesized and applied to fabricate hydrogels through metal crosslinking.Aluminum crosslinking was found to be the best choice for enhancing the strength.This study systematically compared the morphologies,storage modulus,loss factor,continuous shear ramp,self-healing,swelling,in vitro degradation and injectable properties of the fabricated hydrogels,Further,a radar chart is summarized as guidelines to direct the rational selection to meet the specific requirements of further biomedical applications.At the same nanocellulose concentration and after Al^(3+)crosslinking,CNCs hydrogels had strong water holding capacity twice as much as that of CNFs hydrogels.While CNFs hydrogels showed higher hardness and stronger resistance to degradation than that of CNCs.These results provide detailed insights into nanocellulose hydrogels,making it possible to use these guidelines to select hydrogels for desired performance. | Zhongxin Lin Renliang Huang Jiangjiexing Wu Anastasia Penkova Wei Qi Zhimin He Rongxin Su | 2022 | Chinese Journal of Chemical Engineering2022，，10： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员