| 1 | Association of single nucleotide polymorphisms of brain-derived neurotrophic factor gene and multidrug resistance 1 gene to refractory epilepsy in Chinese Han children显示文摘BACKGROUND:There are two hypotheses for the underlying cause of refractory epilepsy:'target'and'transport'.Studies have shown that brain-derived neurotrophic factor(BDNF) is over-expressed in refractory epilepsy.Multidrug resistance 1(MDR1) gene encodes for P-glycoprotein,the primary ATP-binding cassette transporter in the human body.Some single nucleotide polymorphisms of the MDR1 gene have been associated with refractory epilepsy. OBJECTIVE:To investigate the association between BDNF gene C270T polymorphism and MDR1 T-129C polymorphism with refractory epilepsy in Chinese Han children through the use of polymerase chain reaction(PCR)-restriction fragment length polymorphism analysis. DESIGN,TIME AND SETTING:A case-control,genetic association study was performed at the Central Laboratory,Third Xiangya Hospital of Central South University from June 2005 to November 2007. PARTICIPANTS:A total of 84 cases of unrelated children with epilepsy,including 41 cases of refractory epilepsy and 43 cases of drug-responsive epilepsy,were enrolled.An additional 30 healthy,Chinese Han children,whose ages and gender matched the refractory epilepsy patients, were selected as normal controls. METHODS:Venous blood was collected and genomic DNA was extracted from the blood specimens.C270T polymorphism in BDNF gene and T-129C polymorphism in MDR1 gene were genotyped using PCR-restriction fragment length polymorphism analysis.Association analysis using the Ftest and Chi-square test was statistically performed between C270T polymorphism in BDNF gene and T-129C polymorphism in MDR1 gene and refractory epilepsy. MAIN OUTCOME MEASURES:The distribution of genotypes and allele frequencies of C270T polymorphism in BDNF gene and T-129C polymorphism in MDR1 gene. RESULTS:The distribution of CC,CT,and TT genotypes,as well as C and T allele frequencies,in the BDNF gene was not significantly different between the refractory epilepsy group, drug-responsive epilepsy group,or the normal control group(P>0.05).The distribution of TT genotype and T allele frequencies of the MDR1 gene was significantly different in the refractory epilepsy group compared with the drug-responsive epilepsy and normal control groups(P<0.05). Comparison of haplotype combinations demonstrated that there were no significant differences in combinations of TT+CC,TT+CT,TC+CC,and TC+CT among the three groups(P>0.05). CONCLUSION:C270T polymorphism of the BDNF gene was not associated with refractory epilepsy in Chinese Han children,but T-129C polymorphism in the MDR1 gene was associated with refractory epilepsy in Chinese Han children.The TT genotype and T allele frequencies could serve as susceptibility loci for refractory epilepsy.Interactions between C270T in BDNF gene and T-129C in MDR1 gene were not observed in refractory epilepsy in Chinese Han children. | Guangxin Wang Zuocheng Yang Ruifeng Jin Ruopeng Sun | 2009 | Neural Regeneration Research2009，4，11： | 2 |
| 3 | Immunogenic molecules associated with gut bacterial cell walls:chemical structures,immune-modulating functions,and mechanisms显示文摘Interactions between gut microbiome and host immune system are fundamental to maintaining the intestinal mucosal barrier and homeostasis.At the host-gut microbiome interface,cell wall-derived molecules from gut commensal bacteria have been reported to play a pivotal role in training and remodeling host immune responses.In this article,we review gut bacterial cell wall-derived molecules with characterized chemical structures,including peptidoglycan and lipid-related molecules that impact host health and disease processes via regulating innate and adaptive immunity.Also,we aim to discuss the structures,immune responses,and underlying mechanisms of these immunogenic molecules.Based on current advances,we propose cell wall-derived components as important sources of medicinal agents for the treatment of infection and immune diseases. | Ruopeng Yin Tao Wang Huanqin Dai Junjie Han Jingzu Sun Ningning Liu Wang Dong Jin Zhong Hongwei Liu | 2023 | Protein & Cell2023，14，10： | 0 |
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