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| 1 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 2 | 卒中介入治疗培训指南:国际多学会共识文件显示文摘1背景
缺血性卒中是全球人口死亡和残疾的首要原因。很多急性大血管闭塞(emergent large vesselocclusion,ELVO)患者都会遗留长期残疾。事实上,这些颅内大动脉闭塞经常会导致大面积脑损伤,进而造成患者死亡或严重致残。 | Lavine SD Cockroft K Hoh B Bambakidis N Khalessi AA Woo H Riina H Siddiqui A Hirsch JA Chong W Rice H Wenderoth J Mitchell P Coulthard A Signh TJ Phatorous C Khangure M Klurfan P ter Brugge K Iancu D Gunnarsson T Pongpech S Rodesch G Soderman M Taylor A Krings T Orbach D Picard L Suh DC Zheng HQ Jansen O Muto M Szikora I Pierot L Brouwer P Gralla J Renowden S Andersson T Fiehler J Turjman F White P Januel AC Spelle L Kulcsar Z Chapot R Biondi A Dima S Taschner C Szajner M Krajina A Sakai N Matsumaru Y Yoshknura S Ezura M Fujinaka T Iihara K Ishii A Higashi T Hirohata M Hyodo A Ito Y Kawanishi M Kiyosue H Kobayashi E Kobayashi S Kuwayama N Matsumoto Y Miyachi S Murayama Y Nagata I Nakahara I Nemoto S Niimi Y Oishi H Satomi J Satow T Sugiu K Tanaka M Terada T Yamagami H Diaz O Lylyk P Jayaraman MV Patsalides A Gandhi CD Lee SK Abruzzo T Albani B Ansari SA Arthur AS Baxter BW Bulsara KR Chen M Almandoz JE Fraser JF Heck DV Hetts SW Hussain MS Klucznik RP Leslie-Mawzi TM Mack WJ McTaggart RA Meyers PM Mocco J Prestigiacomo CA Pride GL Rasmussen PA Starke RM Sunenshine PJ Tarr RW Frei DF Pabo M Nogueira RG Zaidat OO Jovin T Linfante I Yavagal D Liebeskind D Novakovic R Pongpech S 许岩 孙瑞 郭芮兵 | 2017 | 国际脑血管病杂志2017，25，5： | 2 |
| 3 | 遗传性肥胖与心房颤动的关系显示文摘观察性研究已经确定了体质量指数(body mass index,BMI)与心房颤动之间的关联。然而,从观察性研究中推断因果关系会受到混杂因素、逆因果关系和偏倚的影响。该研究的主要目的是应用BMI的遗传学预测因子评估BMI与心房颤动之间的因果关系。方法:纳入1987-2002年期间在美国、冰岛和荷兰实施的7个前瞻性人群队列研究中基线无心房颤动的欧洲血统个体51 646人, | 刘莉 叶鹏 Chatterjee NA Giulianini F Geelhoed B Lunetta KL Misialek JR Niemeijer MN Rienstra M Rose LM Smith AV Arking DE Ellinor PT Heeringa J Lin H Lubitz SA Soliman EZ Verweij N Alonso A Benjamin EJ Gudnason V Stricker BH van der Harst P Chasman DI Albert CM | 2017 | 中华高血压杂志2017，25，2： | 2 |
| 4 | 血管生成素样蛋白2水平与肾移植术后主动脉僵硬度和死亡率呈正相关显示文摘血管生成素样蛋白2(angiopoietin-like-2,ANGPTL2)是一种分泌性糖蛋白,可诱发血管内皮功能障碍、动脉粥样硬化和心血管病。循环ANGPTL2在慢性肾脏病(chronic kidney disease,CKD)患者中增加,加重患心血管病的风险。 | Desjardins MP Thorin-Trescases N Sidibé A Fortier C DeSerres SA Larivière R Thorin E Agharazii M 陈云 叶鹏 | 2017 | 中华高血压杂志2017，25，12： | 2 |
| 5 | The interplay of osteogenesis and hematopoiesis: expression of a constitutively active PTH/PTHrP receptor in osteogenic cells perturbs the establishment of hematopoiesis in bone and of skeletal stem cells in the bone marrow显示文摘 | KUZNETSOV SA RIMINUCCI M ZIRAN N | 2004 | J Cell Biol2004，167，6： | 1 |
| 6 | Marine ecoregions of the world:a bioregionalization of coastal and shelf areas显示文摘 | Spalding MD Fox HE Allen GR Davidson N Ferdafia ZA Finlayson M Halpern BS Jorge MA Lombana AL Lourie SA Martin KD Manus EC Molnar J Recchia CA Robertson J | | 0，，： | 1 |
| 7 | Autopsy analysis of the implantation site of a permanent selective direct his bundle pacing lead 显示文摘 | CORREA DE SA D D HARDIN N J CRESPO E M | 2012 | Circ Arrhythm Electrophysiol2012，5，： | 1 |
| 8 | Development of nanoscale approaches for ovarian cancer therapeutics and diagnostics显示文摘 | Engelberth SA Hempel N Bergkvist M | 2014 | Crit Rev Oncog2014，19，34： | 1 |
| 9 | Nerve growth factor mRNA and protain levels measured in the same tissue from normal and Alzheimer's disease parietal cortex prain显示文摘 | Scott SA Jette N | 1996 | Res Mol Brain Res1996，42，1： | 1 |
| 10 | The central executioner of apoptosis:multiple connections between protease activation and mitochondria in Fas/ Apo-1/ CD95 and ceramide-induced apoptosis显示文摘 | Susin SA Zamzami N Castedo M | 1997 | J Exp Med1997，186，1： | 1 |
| 11 | Immediate effect of kinesio taping on muscle response in young elite soccer players显示文摘 | de Hoyo M (A)lvarez-Mesa A Sa(n)udo B | 2013 | J Sport Rehabil2013，22，1： | 1 |
| 12 | Osteoclastogen- esis in fibrous dysplasia of bone: in situ and in vitro analysis of IL-6 expression显示文摘 | Riminucci M Kuznetsov SA Cherman N | 2003 | Bone2003，33，3： | 1 |
| 13 | Osteoclastogenesis in fibrous dysplasia of bone: in situ and in vitro analysis of IL-6 expression 显示文摘 | Riminucci M Kuznetsov SA Cherman N | 2003 | Bone2003，33，3： | 1 |
| 14 | Bcl-2 inhibits the mitochondrial release of an apoptogenic protease 显示文摘 | Susin SA Zamzami N Castedo M | 1996 | J Exp Med1996，184，4： | 1 |
| 15 | The central executioner of apoptosis:multiple connections between protease activation and mitochondrial in Fas/APO-1/CD95- and ceramide-induced apoptosis显示文摘 | Susin SA Zamzami N Castedo M | 1997 | J Exp Med1997，186，： | 1 |
| 16 | Subcellular path- ways of 13-endorphin synthesis, processing, and release from immunocytes in inflammatory pain 显示文摘 | Mousa SA Shakibaei M Sitte N | 2004 | Endocrinology2004，145，3： | 1 |
| 17 | The female gubernaculum: role in the embryology and development of the genital tract and in the possible genesis of malformations显示文摘 | Acie'n P Sa'nchez del Campo F Mayol M J | 2011 | Eur J Obstet Gynecol Reprod Biol2011，159，2： | 1 |
| 18 | Evaluation of some mango species by fruit characters and fingerprint显示文摘 | ZAIED N S KHAFAGY SA A SALEH M A | 2007 | J Agric Biol Sci2007，4，： | 1 |
| 19 | Cytotoxic Activity of Kenaf Seed Oils from Supercritical Carbon Dioxide Fluid Extraction towards Human Colorectal Cancer (HT29) Cell Lines显示文摘 | Abd Ghafar SA Ismail M Yazan LS Fakurazi S Ismail N Chan KW Tahir PM | 2013 | Evidence- Based Complementary and Alternative Medicine2013，，： | 1 |
| 20 | Changes in 12-year first-line eradication rate of helicobacter pylori based on triple therapy with proton pump inhibitor,amoxicillin and clarithromycin显示文摘 | Sa~aki M Ogasawara N Utsumi K | 2010 | J Clin Biochem Nutr2010，47，1： | 1 |
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