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| 1 | Occult hepatitis B virus infection among Egyptian blood donors显示文摘AIM:To identify blood donors with occult hepatitis B virus(HBV) infection(OBI) to promote safe blood donation.METHODS:Descriptive cross sectional study was conducted on 3167 blood donors negative for hepatitis B surface antigen(HBsAg),hepatitis C antibody(HCV Ab) and human immunodeficiency virus Ab.They were subjected to the detection of alanine aminotransferase(ALT) and aspartate transaminase(AST) and screening for anti-HBV core antibodies(total) by two different techniques;[Monoliza antibodies to hepatitis B core(Anti-HBc) Plus-Bio-Rad] and(ARC-HBc total-ABBOT).Positive samples were subjected to quantitative detection of antibodies to hepatitis B surface(anti-HBs)(ETI-AB-AUK-3,Dia Sorin-Italy).Serum anti-HBs titers > 10 IU/L was considered positive.Quantitative HBV DNA by real time polymerase chain reaction(PCR)(QIAGEN-Germany) with 3.8 IU/mL detection limit was estimated for blood units with negative serum anti-HBs and also for 32 whose anti-HBs serum titers were > 1000 IU/L.Also,265 recipients were included,34 of whom were followed up for 3-6 mo.Recipients were investigated for ALT and AST,HBV serological markers:HBsAg(ETI-MAK-4,Dia Sorin-Italy),anti-HBc,quantitative detection of anti-HBs and HBV-DNA.RESULTS:525/3167(16.6%) of blood units were positive for total anti-HBc,64% of those were antiHBs positive.Confirmation by ARCHITECT anti-HBc assay were carried out for 498/525 anti-HBc positive samples,where 451(90.6%) confirmed positive.Reactivity for anti-HBc was considered confirmed only if two positive results were obtained for each sample,giving an overall prevalence of 451/3167(14.2%) for total anti-HBc.HBV DNA was quantified by real time PCR in 52/303(17.2%) of anti-HBc positive blood donors(viral load range:5 to 3.5 x 105 IU/mL) with a median of 200 IU/mL(mean:1.8 x 104 ± 5.1 x 104 IU/mL).AntiHBc was the only marker in 68.6% of donors.Univariate and multivariate logistic analysis for identifying risk factors associated with anti-HBc and HBV-DNA positivity among blood donors showed that age above thirty and marriage were the most significant risk factors for prediction of anti-HBc positivity with AOR 1.8(1.4-2.4) and 1.4(1.0-1.9) respectively.Other risk factors as gender,history of blood transfusion,diabetes mellitus,frequent injections,tattooing,previous surgery,hospitalization,Bilharziasis or positive family history of HBV or HCV infections were not found to be associated with positive anti-HBc antibodies.Among anti-HBc positive blood donors,age below thirty was the most significant risk factor for prediction of HBV-DNA positivity with AOR 3.8(1.8-7.9).According to HBV-DNA concentration,positive samples were divided in two groups;group one with HBV-DNA ≥ 200 IU/mL(n = 27) and group two with HBV-DNA < 200 IU/mL(n = 26).No significant difference was detected between both groups as regards mean age,gender,liver enzymes or HBV markers.Serological profiles of all followed up blood recipients showed that,all were negative for the studied HBV markers.Also,HBV DNA was not detected among studied recipients,none developed post-transfusion hepatitis(PTH) and the clinical outcome was good.CONCLUSION:OBI is prevalent among blood donors.Nucleic acid amplification/HBV anti core screening should be considered for high risk recipients to eliminate risk of unsafe blood donation. | Zeinab N Said Manal H El Sayed Iman I Salama Enas K Aboel-Magd Magda H Mahmoud Maged El Setouhy Faten Mouftah Manal B Azzab Heidi Goubran Amal Bassili Gamal E Esmat | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 3 | A review on phase change energy storage: materials and applications显示文摘 | Mohammed M Farid Amar M Khudhair Siddique Ali K Razack Said Al-Hallaj | 2003 | Energy Conversion and Management2003，，9： | 2 |
| 4 | Involvement of electrical coupling in the in vivo ictal epileptiform activity induced by 4-aminopyridine in the neocortex 显示文摘 | Szente M Gajda Z Said Ali K | 2002 | Neuroscience2002，115，4： | 1 |
| 5 | Biotin uptake by human proximal tubular epithelial cells: cellular and molecular aspects 显示文摘 | BALAMURUGAN K VAZIRI ND SAID HM | 2005 | Am J Physiol Renal Physiol2005，288，4： | 1 |
| 6 | Metformin - mediated growth inhibition involves suppression of the IGF - I receptor signaling pathway in human pancreatic cancer cells 显示文摘 | Karnevi E Said K Andersson R | 2013 | BMC Cancer2013，13，： | 1 |
| 7 | Experts' knowledge renewal and maintenance actions effectiveness in high- mix low-volume industries ,using Bayesian approach显示文摘 | SAID A B SHAHZAD M K ZAMAI E | 2016 | Cognition technology & work2016，18，1： | 1 |
| 8 | Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells显示文摘 | LEGRAND D VIGIE K SAID E A | 2004 | Eur J Biochem2004，271，： | 1 |
| 9 | VIP inhibits basal and histamine-stimulated proliferation of human airway smooth muscle cells显示文摘 | Maruno K Absood A Said S1 | 1995 | AmJ Physiol1995，268，6： | 1 |
| 10 | Metformin- mediated growth inhibition involves suppression of the IGF- I receptor signalling pathway in human pancreaticcancer cells显示文摘 | Karnevi E Said K Andersson R | 2013 | BMC Cancer2013，13，： | 1 |
| 11 | The role of SPARC in the TRAMP model of prostatecarcinogenesis and progression显示文摘 | Said N Frierson Jr HF Chernauskas D Conaway M Motamed K Theodorescu D | 2009 | Oncogene2009，，28： | 1 |
| 12 | Optimization liquid chroma- tographic method for determination of malachite green and its metabolites in fish tissues 显示文摘 | Plakas S M E1 Said K R | 1995 | Journal of AOAC International1995，78，6： | 1 |
| 13 | Simulation of the Well-bore Hydraulics While Drilling, Including the Effects ofFluid Influxes and Losses and Pipe Washouts 显示文摘 | Millheim K K Said Sahin Tulga | | SPE11057 - MS0，1，： | 1 |
| 14 | A hypergame approach to crisis decision-making: The 1973 Middle East War显示文摘 | Said A K Hartley D A | 1982 | The Journal of the Operational Research Society1982，33，10： | 1 |
| 15 | Expression of inflammatory modulator COX-2 in pancreatic ductal adenocarcinoma and its rela tionship to pathologic and clinical parameters显示文摘 | Merati K Said SM Andea A | 2001 | AM J Clin Oncol2001，24，5： | 1 |
| 16 | Seismic response of a cablestayed bridge deck under multi-component non-stationary random ground motion 显示文摘 | Allam Said M Datta T K | 2004 | Earthquake Engineering and Structural Dynamics2004，33，3： | 1 |
| 17 | Alteratins of the cyclin-dependent kinase inhibitor p19INK4D is rare in hematopoietic malignancies显示文摘 | Shiahara M Spirin K Said JW | 1996 | Leukemia1996，10，12： | 1 |
| 18 | A hypergame approach to crisis decision-making: the 1973 Middle East War 显示文摘 | Said A K Hartley D A | 1982 | Journal of the Operational Research Society1982，33，10： | 1 |
| 19 | Liquid Chromatographic Determination of Furazolidone in Shrimp显示文摘 | Stehly G R Plakas S M el Said K R | | 0，，04： | 1 |
| 20 | Effect of a heat pipe on Dehumidification of a controlled air space显示文摘 | John K McFarland Sheldon M Jeter Said I Abdel-Khalik | 1996 | ASHRAE Transactions1996，102，1： | 1 |
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