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| 1 | Bt protein expression in the transgenic insect-resistant cotton in China显示文摘According to statistics of International Service for the Acquisition of Agri-biotech Applications(ISAAA),18million farmers in 28 countries planted more than 181million hectares of genetically modified(GM)crops in2014,at an annual growth rate of between 3%–4%,with6.3 million ha more than 2013[1].Among these counties,China ranks the sixth with 3.9 million ha.Globally,27kinds of transgenic crops have been approved.The four main food groups for which GM varieties are | Guoqing Sun Dongling Zhang Rui Zhang Yuan Wang Zhigang Meng Tao Zhou Chenzhen Liang Tao Zhu Sandui Guo | 2016 | Science Bulletin2016，61，20： | 3 |
| 2 | Inheritance of resistance to Helicoverpa armigera of 3 kinds of transgenic Bt strains available in upland cotton in China显示文摘There are 3 kinds of transgenic Bt strains, Shanxi 94-24, Zhongxin 94, and R19, in upland cotton in China. Their transgenic Bt insect-resistance cultivars or hybrids have been developed and grown by farmers. Genetic studies indicate that the resistance of the 3 transgenic Bt cotton strains to Helicoverpa armigera is controlled by one pair of non-allelic dominant genes. Linkage relationship between the resistant genes of R19 and Shanxi 94-24 transgenic Bt strains shows that they may be inserted in the same chromosome. FT hybrids crossed among the 3 strains show that high levels of protection from feeding damage are the same as that of their parents. Therefore, there is no co-suppression phenomenon in many transgenic plants. The results presented here afford a fundamental reliance in developing transgenic Bt insect-resistant cultivars and exploiting the heterosis of hybrids in upland cotton. | TANG Canming SUN Jing ZHU Xiefei GUO Wangzhen ZHANG Tianzhen SHEN Jinliang GAO Congfen ZHOU Weijun CHEN Zhixian GUO Sandui | 2000 | Chinese Science Bulletin2000，45，4： | 3 |
| 3 | Increased lateral root formation by CRISPR/Cas9-mediated editing of arginase genes in cotton显示文摘Dear Editor,Proper development of plant roots is critical for primary physiological functions,including water and nutrient absorption and uptake,physical support,and carbohydrate storage(Zhang et al.,2010).Crop root systems act as the key organ for sensing and response to abiotic and biotic stresses.Previous studies of crop root system development suggest that increased lateral root formation(LRF)could | Yanling Wang Zhigang Meng Chengzhen Liang Zhaohong Meng Yuan Wang Guoqing Sun Tao Zhu Yongping Cai Sandui Guo Rui Zhang Yi Lin | 2017 | Science China(Life Sciences)2017，60，5： | 3 |
| 4 | Integration and inheritance stability of foreign Bt toxin gene in the bivalent insect-resistant transgenic cotton plants显示文摘Genetic and expressional stability of Bt toxin gene is crucial for the breeding of insect-resistant transgenic cotton varieties and their commercialization. Genomic Southern blot analysis of R3, R4 and R5 generations of bivalent transgenic insect-resistant cotton plants was done in order to determine the integration, the copy number and the inheritance stability of Bt toxin gene in the transgenic cotton plants. The results indicated that there was a 4.7 kb positive band in the Southern blot when the genomic DNA of the bivalent transgenic insect-resistant cotton plants and the positive control (the plasmid) were digested with HindⅢ respectively. This result proved that the Bt toxin gene had been integrated into the genome of the cotton in full length. There is only one XhoⅠ restriction site in the Bt toxin gene. Southern blot analysis indicated that many copies of Bt toxin gene had been integrated into the genome of the cotton when the genomic DNA of transgenic plants was digested with XhoⅠ. Among them, there were four copies (about 17.7, 8, 5.5 and 4.7 kb in size) existing in all the tested plants of R3, R4 and R5 generations. The preliminary conclusion was that there were more than four copies of Bt toxin gene integrated into the genome of the cotton, among them, more than one copy can express and inherit steadily. This result provides a scientific basis for the breeding of the bivalent insect-resis- tant transgenic cotton plants and its commercialization. | XIA Lanqin GUO Sandui | 2001 | Chinese Science Bulletin2001，46，16： | 2 |
| 5 | Co-expression of GR79 EPSPS and GAT generates highglyphosate-resistant alfalfa with low glyphosate residues显示文摘Weed competition seriously threatens the yield of alfalfa, the most important forage legume worldwide,thus generating herbicide-resistant alfalfa varieties is becoming a necessary cost-effective strategy toassist farmers for weed control. Here, we report the co-expression of plant codon-optimized forms ofGR79 EPSPS (pGR79 EPSPS) and N-acetyltransferase (pGAT) genes, in alfalfa, via Agrobacterium-mediated transformation. We established that the pGR79 EPSPS-pGAT co-expression alfalfa lines were able totolerate up to tenfold higher commercial usage of glyphosate and produced approximately ten timeslower glyphosate residues than the conventional cultivar. Our findings generate an elite herbicideresistant germplasm for alfalfa breeding and provide a promising strategy for developing high-glyphosate-resistant and low-glyphosate-residue forages. | Yingying Meng Wenwen Zhang Zhaoming Wang Feng Yuan Sandui Guo Hao Lin Lifang Niu | 2023 | aBIOTECH2023，4，4： | 0 |
| 6 | Establishment of a genetic transformation system for maize inbred P9-10显示文摘It was found that the supplement of 10-4 mol/L AgNO3 in N6 medium enhanced the induction of type Ⅰembryogenic calli from immature embryos of maize inbred P9-10. After high-osmotic treatment, the induced calli was taken as transformation recipient to be bombarded with plasmid pMG6 carrying synthetic Bt gene by PDS-1000/He genegun. A total of 14 resistant calli were obtained after screening subsequently on the mediums with gradually increasing selective pressure. 10 plants were regenerated from the resistant calli on 3 different induction media,and 8 out of the 10 regenerated plants were confirmed to be integrated with Bt gene by PCR and Southern blotting analysis. Results of ELISA showed that the Bt-protein content in the leaves of the transgenic plants varied between 20—200 ng/g fresh weight. | Fengyong Zhou Guoying Wang Youju Xie Hongzhi Cui Sandui Guo Jingruil Dai | 1999 | Chinese Science Bulletin1999，44，7： | 0 |
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