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| 1 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 2 | New ways to measure intraocular pressure显示文摘 | Mohammed K ElMallah Sanjay G Asrani | 2008 | Current Opinion in Ophthalmology2008，，2： | 2 |
| 3 | Integrated flight propulsion control system design based on a centralized approach显示文摘 | Sanjay G Duane L M Bullard R E | 1991 | Journal of Guidance Control and Dynamics1991，14，1： | 1 |
| 4 | Antifungal activity of Agave americana leaf extract against Alternaria brassicae, causal agent of Alter naria blight of Indian mustard (Brassica juncea )显示文摘 | Sanjay G Ashok K | 2009 | Arch Phytopathol Plant Protect2009，42，4： | 1 |
| 5 | Geo-metric modelling of end mills显示文摘 | Puneet Tandon Phalguni Gupta Sanjay G Dhande | 2005 | Computer - Aided Design & Applications2005，2，14： | 1 |
| 6 | Green tea polyphenol epigallocateehin-3-galhte differentially modulates nuclear factor kB in cancer cells versus normal cells显示文摘 | Sanjay G Hasan M | 2000 | Arch Biochem Biophys2000，376，： | 1 |
| 7 | Patterns of litterfal and return of nutrients across anthropogenic disturbance gradients in three subalpine forests of west Himalaya, India 显示文摘 | Sanjay G Rawal R S Dhar U | 2017 | Journal of Forest Research2017，14，： | 1 |
| 8 | Biochemical studies of citric acid production and accumulation by AspergiUus niger mutants 显示文摘 | Sanjay G Chandra BS | 2002 | Biotechnology letters2002，5，18： | 1 |
| 9 | MapReduce= Simplified data processing on large clusters显示文摘 | Jeffrey D Sanjay G | 2008 | Communications of the ACM2008，51，1： | 1 |
| 10 | Small (≤2 cm) subpleural pulmonary lesions:short-versus long-needle-path CT-guided biopsy-comparision of diagnostic yields and complications显示文摘 | Sanjay G Savitri K Lyle D | 2005 | Radiol2005，234，： | 1 |
| 11 | Instantaneous thermal efficiency of an active solar still显示文摘 | Tiwari G N Kumar Sanjay Sharma P B | 1996 | Applied Thermal Engineering1996，16，2： | 1 |
| 12 | Pain management in polycystic kidney disease显示文摘 | Zahid HB Sanjay G Carol AW | 2001 | Kid Int2001，60，5： | 1 |
| 13 | Recovery of diosgenin from dioscorea rhizomes using aqueous hydrotropic solutions of sodium cumene sulfonate 显示文摘 | Sanjay P Mishra Vilas G Gaikar | 2004 | Ind Eng Chem Res2004，43，： | 1 |
| 14 | MapReduce: simplified data pro- cessing on large clusters显示文摘 | Jeffrey D Sanjay G | 2008 | Communications of the ACM2008，51，1： | 1 |
| 15 | Choices in Pain Management Following Thoracotomy显示文摘 | Christinc P A Sanjay G | 1999 | Chest1999，115，： | 1 |
| 16 | Acid activated montmorillonite: an efficient immobilization support for improving reusability, storage stability and operational stability of enzymes 显示文摘 | SANJAY G SUGUNAN S | 2008 | Journal of Porous Materials2008，15，3： | 1 |
| 17 | MapReduce:Simplified Date Processing on Large Clusters显示文摘 | Jeffrey D Sanjay G | 2008 | Communications of the ACM2008，51，1： | 1 |
| 18 | DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex显示文摘 | Sanjay A Fu J Kreibic G | 1998 | The Jour- nal of Biological Chemistry1998，273，26： | 1 |
| 19 | Inhibition of p38 MAP kinase as a therapeutic strategy显示文摘 | JOHN L C SANJAY K DON E G | 2000 | Immunopharmacology2000，47，23： | 1 |
| 20 | MapReduce:Simplified Data Processing on Large Clusters显示文摘 | Dean J Sanjay G | 2008 | Communications of the ACM2008，51，1： | 1 |
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