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| 1 | Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation显示文摘AIM:To provide further insight into the characterization of mucosa-associated Escherichia coli(E.coli)isolated from the colonic mucosa of cancer patients.METHODS:Phylogroups and the presence of cyclomodulin-encoding genes of mucosa-associated E.coli from colon cancer and diverticulosis specimens weredetermined by PCR.Adhesion and invasion experiments were performed with I-407 intestinal epithelial cells using gentamicin protection assay.Carcinoembryonic antigen-related cell adhesion molecule 6(CEACAM6)expression in T84 intestinal epithelial cells was measured by enzyme-linked immunosorbent assay and by Western Blot.Gut colonization,inflammation and procarcinogenic potential were assessed in a chronic infection model using CEABAC10 transgenic mice.Cell proliferation was analyzed by real-time mRNA quantification of PCNA and immunohistochemistry staining of Ki67.RESULTS:Analysis of mucosa-associated E.coli from colon cancer and diverticulosis specimens showed that whatever the origin of the E.coli strains,86%of cyclomodulin-positive E.coli belonged to B2 phylogroup and most harbored polyketide synthase(pks)island,which encodes colibactin,and/or cytotoxic necrotizing factor(cnf)genes.In vitro assays using I-407 intestinal epithelial cells revealed that mucosa-associated B2 E.coli strains were poorly adherent and invasive.However,mucosa-associated B2 E.coli similarly to Crohn’s disease-associated E.coli are able to induce CEACAM6expression in T84 intestinal epithelial cells.In addition,in vivo experiments using a chronic infection model of CEACAM6 expressing mice showed that B2 E.coli strain11G5 isolated from colon cancer is able to highly persist in the gut,and to induce colon inflammation,epithelial damages and cell proliferation.CONCLUSION:In conclusion,these data bring new insights into the ability of E.coli isolated from patients with colon cancer to establish persistent colonization,exacerbate inflammation and trigger carcinogenesis. | Jennifer Raisch Emmanuel Buc Mathilde Bonnet Pierre Sauvanet Emilie Vazeille Amélie de Vallée Pierre Déchelotte Claude Darcha Denis Pezet Richard Bonnet Marie-Agnès Bringer Arlette Darfeuille-Michaud | 2014 | World Journal of Gastroenterology2014，20，21： | 12 |
| 2 | Association of colorectal cancer with pathogenic Escherichia coli: Focus on mechanisms using optical imaging显示文摘AIM: To investigate the molecular or cellular mechanisms related to the infection of epithelial colonic mucosa by pks-positive Escherichia coli(E. coli) using optical imaging.METHODS: We choose to evaluate the tumor metabolic activity using a fluorodeoxyglucose analogue as 2-deoxyglucosone fluorescent probes and to correlate it with tumoral volume(mm^3). Inflammation measuring myeloperoxidase(MPO) activity and reactive oxygen species production was monitored by a bioluminescent(BLI) inflammation probe and related to histological examination and MPO levels by enzyme-linked immunosorbent assay(ELISA) on tumor specimens. The detection and quantitation of these two signals were validated on a xenograft model of human colon adenocarcinoma epithelial cells(HCT116) in nude mice infected with a pks-positive E. coli. The inflammatory BLI signal was validated intra-digestively in the colitisCEABAC10 DSS models, which mimicked Crohn's disease. RESULTS: Using a 2-deoxyglucosone fluorescent probe, we observed a high and specific HCT116 tumor uptake in correlation with tumoral volume(P = 0.0036). Using the inflammation probe targeting MPO, we detected a rapid systemic elimination and a significant increase of the BLI signal in the pks-positive E. coli-infected HCT116 xenograft group(P < 0.005). ELISA confirmed that MPO levels were significantly higher(1556 ± 313.6 vs 234.6 ± 121.6 ng/m L P = 0.001) in xenografts infected with the pathogenic E. coli strain. Moreover, histological examination of tumor samples confirmed massive infiltration of pks-positive E. coli-infected HCT116 tumors by inflammatory cells compared to the uninfected group. These data showed that infection with the pathogenic E. coli strain enhanced inflammation and ROS production in tumors before tumor growth. Moreover, we demonstrated that the intra-digestive monitoring of inflammation is feasible in a reference colitis murine model(CEABAC10/DSS).CONCLUSION: Using BLI and fluorescence optical imaging, we provided tools to better understand hostpathogen interactions at the early stage of disease, such as inflammatory bowel disease and colorectal cancer. | Julie Veziant Johan Gagnière Elodie Jouberton Virginie Bonnin Pierre Sauvanet Denis Pezet Nicolas Barnich Elisabeth Miot-Noirault Mathilde Bonnet | 2016 | World Journal of Clinical Oncology2016，7，3： | 10 |
| 3 | Role of frozen section assessment for intraductal papillary and mucinous tumor of the pancreas显示文摘Intraductal papillary mucinous neoplasms(IPMN) of the pancreas include a spectrum of dysplasia ranging from minimal mucinous hyperplasia to invasive carcinoma and are extensive tumors that often spread along the ductal tree.Several studies have demonstrated that preoperative imaging is not accurate enough to adapt the extent of pancreatectomy and have suggested routinely using frozen sectioning(FS) to evaluate the completeness of resection and also to check if ductal dilatation is active or passive,in order to avoid an excessive pancreatic resection.Separate main duct and branch duct analysis is needed due to the difference in the natural history of the disease.FS accuracy averages 95%.Eroded epithelium on the main duct,severe ductal inflammation mimicking dysplasia and reactive epithelial changes secondary to obstruction can lead to inappropriate FS results.FS results change the planned extent of resection in up to 30% of cases.The optimal cut-off leading to extend pancreatectomy is not consensual and our standard option is to extend pancreatec-tomy if FS reveals:(1) at least IPMN adenoma on the main duct;or(2) at least borderline IPMN on branch ducts;or(3) invasive carcinoma.However,the decision to extend resection must be taken after a multidisciplinary discussion since it does not exclusively depend on the FS result but also on age,general condition and expected prognosis after resection.The main limitation of using FS is the existence of discontinuous('skip') lesions which account for approximately 10% of IPMN in surgical series and can lead to reoperation in up to 8% of cases. | Alain Sauvanet Anne Couvelard Jacques Belghiti | 2010 | World Journal of Gastrointestinal Surgery2010，2，10： | 5 |
| 4 | Resection of intrahepatic cholangiocarcinoma: a Western experience显示文摘 | Alain Valverde Nicolas Bonhomme Olivier Farges Alain Sauvanet Jean F. Flejou Jacques Belghiti | 1999 | Journal of Hepato - Biliary - Pancreatic Surgery1999，，2： | 3 |
| 5 | Seven hundred forty-seven hepatectomies in the 1990s: an update to evaluate the actual risk of liver resection 1 1 No competing interests declared.显示文摘 | Jacques Belghiti Kazuhiro Hiramatsu Stéphane Benoist Pierre Philippe Massault Alain Sauvanet Olivier Farges | 2000 | Journal of the American College of Surgeons2000，，1： | 3 |
| 6 | Seven hundred forty-seven hepatectomies in the 1990s: an update to evaluate the actual risk of liver resection 1 1 No competing interests declared.显示文摘 | Jacques Belghiti Kazuhiro Hiramatsu Stéphane Benoist Pierre Philippe Massault Alain Sauvanet Olivier Farges | 2000 | Journal of the American College of Surgeons2000，，1： | 3 |
| 7 | ENETS Consensus Guidelines for the Management of Patients with Digestive Neuroendocrine Neoplasms: Functional Pancreatic Endocrine Tumor Syndromes显示文摘 | Jensen Robert T Cadiot Guillaume Brandi Maria L De Herder Wouter W Kaltsas Gregory Komminoth Paul Scoazec Jean-yves Salazar Ramon Sauvanet Alain Kianmanesh Reza | 2012 | Neuroendocrinology2012，，2： | 3 |
| 8 | Medial pancreatectomy: A multi-institutional retrospective study of 53 patients by the French Pancreas Club显示文摘 | Alain Sauvanet Christian Partensky Bernard Sastre Jean-Fran?ois Gigot Pierre-Louis Fagniez Jean-Jacques Tuech Bertrand Millat Stéphane Berdah Bertrand Dousset Daniel Jaeck Yves-Patrice Le Treut Christian Letoublon | 2002 | Surgery2002，，5： | 2 |
| 9 | Right Portal Vein Ligation is as Efficient as Portal Vein Embolization to Induce Hypertrophy of the Left Liver Remnant显示文摘 | B. Aussilhou M. Lesurtel A. Sauvanet O. Farges S. Dokmak N. Goasguen A. Sibert V. Vilgrain J. Belghiti | 2008 | Journal of Gastrointestinal Surgery2008，，2： | 2 |
| 10 | Right portal vein ligation: a new planned two-step all-surgical approach for complete resection of primary gastrointestinal tumors with multiple bilateral liver metastases显示文摘 | Reza Kianmanesh Olivier Farges Eddie K Abdalla Alain Sauvanet Philippe Ruszniewski Jacques Belghiti | 2003 | Journal of the American College of Surgeons2003，，1： | 2 |
| 11 | Natural History of Intraductal Papillary Mucinous Tumors of the Pancreas: Actuarial Risk of Malignancy显示文摘 | Philippe Lévy Vincent Jouannaud Dermot O’Toole Anne Couvelard Marie Pierre Vullierme Laurent Palazzo Alain Aubert Philippe Ponsot Alain Sauvanet Frédérique Maire Olivia Hentic Pascal Hammel Philippe Ruszniewski | 2006 | Clinical Gastroenterology and Hepatology2006，，4： | 2 |
| 12 | Pancreaticoduodenectomy with Mesentericoportal Vein Resection for Adenocarcinoma of the Pancreatic Head显示文摘 | Nicolas Carrère MD Alain Sauvanet MD Diane Goere MD Reza Kianmanesh MD PhD Marie-Pierre Vullierme MD Anne Couvelard MD PhD Philippe Ruszniewski MD Jacques Belghiti MD | 2006 | World Journal of Surgery2006，，8： | 2 |
| 13 | Portal Vein Embolization Before Right Hepatectomy: Prospective Clinical Trial显示文摘 | Olivier Farges Jacques Belghiti Reza Kianmanesh Jean Marc Regimbeau Roberto Santoro Valérie Vilgrain Alban Denys Alain Sauvanet | 2003 | Annals of Surgery2003，，2： | 2 |
| 14 | Selective Policy of No Drain after Pancreaticoduodenectomy Is a Valid Option in Patients at Low Risk of Pancreatic Fistula: A Case-Control Analysis显示文摘 | Chetana Lim Safi Dokmak Fran?ois Cauchy Beatrice Aussilhou Jacques Belghiti Alain Sauvanet | 2013 | World Journal of Surgery2013，，5： | 2 |
| 15 | Pancreatic fistula after a pancreaticoduodenectomy for ductal adenocarcinoma and its association with morbidity: a multicentre study of the F rench S urgical A ssociation显示文摘 | Pietro Addeo Jean Robert Delpero Francois Paye Elie Oussoultzoglou Pascal R Fuchshuber Alain Sauvanet Antonio Sa Cunha Yves Patrice Le Treut Mustapha Adham Jean‐Yves Mabrut Laurence Chiche Philippe Bachellier | 2014 | HPB2014，，1： | 2 |
| 16 | Portal Triad Clamping or Hepatic Vascular Exclusion for Major Liver Resection: A Controlled Study显示文摘 | Jacques Belghiti Roger Noun Evelyne Zante Thierry Ballet Alain Sauvanet | 1996 | Annals of Surgery1996，，2： | 2 |
| 17 | Laparoscopic left lateral resection is the gold standard for benign liver lesions: a case–control study显示文摘 | Safi Dokmak Vikram Raut Béatrice Aussilhou Fadhel Samir Ftériche Olivier Farges Alain Sauvanet Jacques Belghiti | 2014 | HPB2014，，2： | 2 |
| 18 | Major Hepatectomy for the Treatment of Complex Bile Duct Injury显示文摘 | Alexis Laurent Alain Sauvanet Olivier Farges Thierry Watrin Emmanuel Rivkine Jacques Belghiti | 2008 | Annals of Surgery2008，，1： | 2 |
| 19 | Continuous Versus Intermittent Portal Triad Clamping for Liver Resection: A Controlled Study显示文摘 | Jacques Belghiti Roger Noun Robert Malafosse Pascal Jagot Alain Sauvanet Filippo Pierangeli Jean Marty Olivier Farges | 1999 | Annals of Surgery1999，，： | 2 |
| 20 | A new technique of side to side caval anastomosis during orthotopic hepatic transplantation without inferior vena caval occlusion显示文摘 | Belghiti J Panis Y Sauvanet A | 1992 | Sung Gynecol Obstet1992，175，3： | 1 |
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