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| 1 | Genetic analysis of 'all-fish' growth hormone gene transferred carp (Cyprinus carpio L.) and its F_1 generation显示文摘Recornbinant 'all-fish' growth hormone gene (GH) was microinjected into the fertilized eggs of carp. A comparison between the growth traits of transgenics and non-transgenics was carried out, and the transgenic individuals with significant 'fast-growing' effect were successfully gained. A comparison on the reproductivities was also given out between the transgenics and their non-transgenic siblings, and showed that the reproductive capacity of transgenics was substantially equivalent to those of the non-transgenics. On the other hand, the genetic separation and the characteristic distribution of the FI generation were genetically analyzed, which gave solid evidence for the hypothesis that 2-3 chromosomes are integrated with trans-gene. In addition, the distinct biological effects for multi-site-integrated transgenes were further discussed. The present study opens a door for the breeding of 'fast-growing' transgenic fish. | WANG Yaping, HU Wei, WU Gang,SUN Yonghua, CHEN Shangping, ZHANG Fuying,ZHU Zuoyan, FENG Jianxin & ZHANG Xirui1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2. Henan Institute of Aquaculture, Zhengzhou 450044, China | 2001 | Chinese Science Bulletin2001，46，14： | 23 |
| 2 | Synthesis and characterization of yttrium hydroxide and oxide microtubes显示文摘有六角形的阶段 Y 的六角形的微试管(哦)3 被一个灵巧的 solvothermal 方法成功地经由一条水晶转变线路当表面活化剂或模板不在时为 12 h 在 160 ° C 综合。扫描电子显微镜学(SEM ) 结果证明准备六角形的微试管与 50 100 nm, ∼1 μ m 的一条外部直径,并且长度在十测微计上面去的墙厚度。立方的阶段 Y 2 O 3 微试管在 Y 的热处理以后被获得(哦)3 在为 4 h 的 700 ° C 的微试管。Eu 3+-doped Y 2 有在 610 nm 的强壮的红排放的微试管也是的 O 3 准备了,他们的光致发光(PL ) 性质被学习。为微试管的形成的可能的机制简短被讨论。 | WANG Shijin ZHONG Shengliang WEN Zubiao WANG Yuling WANG Shangping CHEN Jianjun XU Rong LUO Longfei | 2009 | Rare Metals2009，28，5： | 5 |
| 3 | Time course of foreign gene integration and expression in transgenic fish embryos显示文摘Using a nuclear transplantation approach, the integration and expression of the green fluorescent protein (GFP) gene in the embryogenesis of transgenic loach (Misgurnus anguillicaudatus Cantor) have been studied. The GFP gene expression is first observed at the gastrula stage, which is consistent with the initiation of cell differentiation of fish embryos. The time course of the foreign gene expression is correlated with the regulatory sequences. The expression efficiency also depends on the gene configuration: the expression of pre-integrating circular plasmid at early embryos is higher than that of the linear plasmid. The integration of the GFP gene is first detected at the blastula stage and lasts for quite a long period. When two types of different plasmids are co-injected into fertilized eggs, the behavior of their integration and expression is not identical. | Haobin Zhao Shangping Chen Yonghua Sun Yaping Wang Zuoyan Zhu | 2000 | Chinese Science Bulletin2000，45，8： | 5 |
| 4 | Nuclear transplantation in different strains of zebrafish显示文摘Single later blastula nuclei from AB strain of zebrafish (Danio rerio) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were distinctly different from those of the nuclear recipient fish. It confirmed that the genetic material originated from nuclear donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important developmental problems such as gene function, the regulation of gene expression during animal development, the developmental potential of a nucleus and the interactions between the donor nucleus and the recipient cytoplasm, etc. | Wei Hu Yaping Wang Shangping Chen Zuoyan Zhu | 2002 | Chinese Science Bulletin2002，47，15： | 4 |
| 5 | Factors influencing the occupational well-being of experienced nurses显示文摘Objectives:To determine the factors influencing the occupational well-being of experienced nurses.Methods:We interviewed eight experienced nurses using an exploratory,semi-structured interview.Results:The interviews revealed five themes that influenced occupational well-being in experienced nurses:(1)external occupational recognition;(2)internal career expectation and occupational value;(3)workenvironment;(4)family support;and(5)occupationalplanningand payment.When the nurses were not recognized by patients or clients,their family,colleague and leaders,their well-being was negatively impacted.However,the harmonious work environments and friendly interpersonal relationships positively impacted their well-being.Conclusion:By identifying the factors that contribute to a nurse's occupational well-being,the nursing management is better able to address the nurse's needs to maintain a positive well-being.This in turn will decrease the burnout and increase retention of experienced nurses,which will raise the quality of patient care. | Shangping Zhao Ling Liu Hong Chen | 2015 | International Journal of Nursing Sciences2015，2，4： | 3 |
| 6 | VIRTUAL EXPERIMENTAL ANALYSIS ON CLEANING ELEMENT OF SUGARCANE HARVESTER显示文摘The laws of influence of different factors have been analyzed in order to enhance the working efficiency and fatigue life of the cleaning element in brush shape of the sugarcane harvester.Based on the principle of orthogonal experiment design, the virtual-orthogonal-experimental analysis for the cleaning element is carried out on the finite element analysis (FEA) software-ANSYS after analyzing the nonlinear structural behavior in the working procedure. The results are analyzed with the overall balancing method, and then the optimal combination is got, which is made up of different levels of different factors. Also the optimal combination of design parameters of the cleaning element received from the virtual experimental analysis is conducted an experiment to confirm that the virtual analysis model and results are right, and the effect of factors on the function of the cleaning element is obtained by more analysis and further optimizing. | Ma Fanglan Li Shangping He Yulin Meng Yanmei Chen Weixu | 2005 | Chinese Journal of Mechanical Engineering2005，18，4： | 2 |
| 7 | Research on the orthogonal experiment of numeric simulation of macromolecule-cleaning element for sugarcane harvester显示文摘 | Yanmei Meng Yuanling Chen Shangping Li | 2009 | Materials andDesign2009，30，： | 1 |
| 8 | Optimizing the Gaussian kernel function with the formulated kernel target alignment criterion for two-class pattern classification 显示文摘 | Zhong Shangping Chen Daya Xu Qiaofen | 2013 | Paltern Recognition2013，46，7： | 1 |
| 9 | Solvothermal synthesis of disk-like YF3 superstructures 显示文摘 | Wang Shijin Xu Hualan Chen Xiangshu Zhong Shengliang Jiang Jianwen Huang Yixiang Wang Shangping Xu Rong | 2008 | Journal of Crystal Growth2008，310，22： | 1 |
| 10 | Investigation of Electrical Parameters of Fresh Water and Produced Mixed Injection in High-Salinity Reservoirs显示文摘Assuming a reservoir with a typical salt-lake background in the Qaidam Basin as a testbed,in this study the var-iation law of the rock electrical parameters has been determined through water displacement experiments with different salinities.As made evident by the results,the saturation index increases with the degree of water injec-tion.When the salinity of the injected water is lower than 80000 ppm,the resistivity of the rock samplefirst decreases,then it remains almost constant in an intermediate stage,andfinally it grows,thereby giving rise to a‘U’profile behavior.As the salinity decreases,the water saturation corresponding to the inflection point of the resistivity becomes lower,thereby leading to a wider‘U’type range and a higher terminal resistivity.For dif-ferent samples,higher initial resistivity of the sample in the oil-bearing state,and higher resistivity after low-sali-nity water washing are obtained when a thicker lithology is considered. | Jun Li Guofeng Cheng Hongwei Xiao Xiang Li Lizhi Wang Hui Xu Yu Wang Nannan Liu Shangping Chen Xing Shi | 2023 | Fluid Dynamics & Materials Processing2023，19，10： | 0 |
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