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| 1 | clusterProfiler 4.0:A universal enrichment tool for interpreting omics data显示文摘Functional enrichment analysis is pivotal for interpreting highthroughput omics data in life science.It is crucial for this type of tool to use the latest annotation databases for as many organisms as possible.To meet these requirements,we present here an updated version of our popular Bioconductor package,clusterProfiler 4.0.This package has been enhanced considerably compared with its original version published 9 years ago.The new version provides a universal interface for functional enrichment analysis in thousands of organisms based on internally supported ontologies and pathways as well as annotation data provided by users or derived from online databases.It also extends the dplyr and ggplot2 packages to offer tidy interfaces for data operation and visualization.Other new features include gene set enrichment analysis and comparison of enrichment results from multiple gene lists.We anticipate that clusterProfiler 4.0 will be applied to a wide range of scenarios across diverse organisms. | Tianzhi Wu Erqiang Hu Shuangbin Xu Meijun Chen Pingfan Guo Zehan Dai Tingze Feng Lang Zhou Wenli Tang Li Zhan Xiaocong Fu Shanshan Liu Xiaochen Bo Guangchuang Yu | 2021 | The Innovation2021，2，3： | 40 |
| 2 | Ginkgo biloba extract improved cognitive and neurological functions of acute ischaemic stroke:a randomised controlled trial显示文摘Purpose To evaluate the efficacy and safety of Ginkgo biloba extract(GBE)in acute ischaemic stroke and its impact on the recurrence of vascular events.Methods We conducted a multicentre,prospective,randomised,open label,blinded,controlled clinical trial enrollingpatients with an onset of acute stroke within 7 days from five hospitals in China Jiangsu Province.Participants were assigned to the GBE group(450 mg GBE with 100 mg aspirin daily)or the control group(100 mg aspirin daily)for 6 months.The primary outcome was the decline in the Montreal Cognitive Assessment score at 6 months.Secondary outcomes were other neuropsychological tests of cognitive and neurological function,the the incidence of adverse events and vascular events.results 348 patients were enrolled:179 in the GBE group and 169 in the control group.With 18 patients lost to follow-up,the dropout rate was 5.17%.Admission data between two groups were similar,but in the GBE group there was a marked slow down in the decline in the Montreal Cognitive Assessment scores(−2.77±0.21 vs−1.99±0.23,P=0.0116(30 days);−3.34±0.24 vs−2.48±0.26,P=0.0165(90 days);−4.00±0.26 vs−2.71±0.26,P=0.0004(180 days))compared with controls.The National Institutes of Health Stroke Scale scores at 12 and 30 days,the modified Rankin Scale scores for independent rate at 30,90 and 180 days,and the Barthel Index scores at 30,90 and 180 days in the GBE group were significantly improved compared with controls.Improvements were also observedin GBE groups for Mini-Metal State Examination scores of 30,90 and 180 days,Webster's digit symbol test scores at 30 days and Executive Dysfunction Index scores at 30 and 180 days.No significant differences were seen in the incidence of adverse events or vascular events.conclusions We conclude that GBE in combination with aspirin treatment alleviated cognitive and neurological deficits after acute ischaemic stroke without increasing the incidence of vascular events. | Shanshan Li Xinjiang Zhang Qi Fang Junshan Zhou Meijuan Zhang Hui Wang Yan Chen Biyun Xu Yanfeng Wu Lai Qian Yun Xu | 2017 | Stroke & Vascular Neurology2017，2，4： | 25 |
| 3 | Ischemic postconditioning alleviates lung injury and maintains a better expression of aquaporin-1 during cardiopulmonary bypass显示文摘 | Cheng Chi Li Shanshan Wang Yong Chen Song You Lu Zhang Hong | 2014 | Chinese Medical Journal2014，，23： | 20 |
| 4 | Healthy function and high valued utilization of edible fungi显示文摘Edible fungi are large fungi with high added value that can be utilized as resources.They are rich in high-quality protein,carbohydrate,various vitamins,mineral elements and other nutrients,and are characterized by high protein,low sugar,low fat and low cholesterol.In addition,edible fungi contain a variety of bioactive substances,such as polysaccharides,dietary fiber,steroids,polyphenols,and most of these compounds have antioxidant,anti-tumor and other physiological functions.This review comprehensively discusses the bioactive components and functional characteristics of edible fungi(such as antioxidant,anti-aging,hypolipidemic activities,etc.).Then the recent developments and prospect in the high-valued utilization of edible fungi are discussed and summarized.The objective of this review is to improve the understanding of health-promoting properties of edible fungi,and provide reference for the industrial production of edible fungi-based health products. | Yanrong Zhang Dawei Wang Yuetong Chen Tingting Liu Shanshan Zhang Hongxiu Fan Hongcheng Liu Yu Li | 2021 | Food Science and Human Wellness2021，10，4： | 20 |
| 5 | Acupuncture at Waiguan(SJ5) and sham points influences activation of functional brain areas of ischemic stroke patients: a functional magnetic resonance imaging study显示文摘Most studies addressing the specificity of meridians and acupuncture points have focused mainly on the different neural effects of acupuncture at different points in healthy individuals. This study examined the effects of acupuncture on brain function in a pathological context. Sixteen patients with ischemic stroke were randomly assigned to true point group(true acupuncture at right Waiguan(SJ5)) and sham point group(sham acupuncture). Results of functional magnetic resonance imaging revealed activation in right parietal lobe(Brodmann areas 7 and 19), the right temporal lobe(Brodmann area 39), the right limbic lobe(Brodmann area 23) and bilateral occipital lobes(Brodmann area 18). Furthermore, inhibition of bilateral frontal lobes(Brodmann area 4, 6, and 45), right parietal lobe(Brodmann areas 1 and 5) and left temporal lobe(Brodmann area 21) were observed in the true point group. Activation in the precuneus of right parietal lobe(Brodmann area 7) and inhibition of the left superior frontal gyrus(Brodmann area 10) was observed in the sham group. Compared with sham acupuncture, acupuncture at Waiguan in stroke patients inhibited Brodmann area 5 on the healthy side. Results indicated that the altered specificity of sensation-associated cortex(Brodmann area 5) is possibly associated with a central mechanism of acupuncture at Waiguan for stroke patients. | Ji Qi Junqi Chen Yong Huang Xinsheng Lai Chunzhi Tang Junjun Yang Hua Chen Shanshan Qu | 2014 | Neural Regeneration Research2014，9，3： | 19 |
| 6 | Construction and Identification of a Human Liver Specific MicroRNA Eukaryotic Expression Vector显示文摘MiR-122 is one of the non-coding RNAs which showed its effects on the lipo-metablism,virus infection and HCC forming through regulation of liver gene expression.Its eukaryotic expression vector was constructed by using pSuper which was widely applied in the siRNA expression.The precursor of human miR-122 gene was amplified by polymerase chain reaction(PCR)from the human genomic DNA.The positive clones were screened by PCR and restriction enzyme digestion.The new expression vector of miR-122 was named pHsa-m122.PHsa-m122 and its controls were transfected to HepG2 cells.The miR-122 expression activity was evaluated by GFP122i sensor reporter plasmid through fluorescence detection and Western blot.It was shown that the fluorescence intensity of GFP122si and pHsa-m122 co-transfection group was weaker than that of the controls,so the functional activity of expressed miR-122 was detected.When HepG2 cells were co-transfected with HBV1.3 and pHsa-m122 plasmids, the results showed miR-122 may down-regulate the gene expression of HBV.The human liver specific microRNA eukaryotic expression vector of miR-122 was constructed successfully,which may facilitate further study of its function in the development of liver virus infection diseases and HCC. | Shanshan Chen Ming Ni Bing Yu Tingting Lv Mengji Lu Feili Gong | 2007 | Cellular & Molecular Immunology2007，4，6： | 18 |
| 7 | Role of contrast-enhanced ultrasonography in percutaneous radiofrequency ablation of liver metastases and efficacy evaluation显示文摘Objective: To retrospectively investigate the role of contrast-enhanced ultrasonography (CEUS) in percutaneous radiofrequency ablation (RFA) in patients with liver metastases and evaluate the therapeutic efficacy of RFA assisted by CEUS. Methods: From May 2004 to September 2010, 136 patients with 219 liver metastatic lesions received CEUS examination 1 h before RFA (CEUS group), and other 126 patients with 216 lesions without CEUS examination in the earlier period were served as a historical control group. The mean tumor size was 3.2 cm and the mean tumor number was 1.6 in the CEUS group, while 3.4 cm and 1.7 in the control group, respectively (P>0.05). The clinical characteristics, recurrence results and survival outcomes were compared between two groups. Results: In the CEUS group, two isoechoic tumors were not demonstrated on unenhanced ultrasonography (US), and 63 (47%) of 134 tumors examined with CEUS were 0.3 cm larger than with unenhanced US. Furthermore, in 18.4% of 136 patients, additional 1-3 tumors were detected on CEUS. The CEUS group showed higher early tumor necrosis and lower intrahepatic recurrence than the control group. The 3-year overall survival (OS) rate and the 3-year local recurrence-free survival (LRFS) rate in the CEUS group were 50.1% and 38.3%, in contrast to 25.3% and 19.3% in the control group, respectively (P=0.002 and P<0.001). Conclusions: CEUS provides important information for RFA treatment in patients with liver metastases and better therapeutic effect could be attained. | Jie Wu Wei Yang Shanshan Yin Jinyu Wu Wei Wu Kun Yan Minhua Chen | 2013 | Chinese Journal of Cancer Research2013，25，2： | 17 |
| 8 | Enhanced allelopathy and competitive ability of invasive plant Solidago canadensis in its introduced range显示文摘Aims Why invasive plants are more competitive in their introduced range than native range is still an unanswered question in plant invasion ecology.Here,we used the model invasive plant Solidago canaden-sis to test a hypothesis that enhanced production of allelopathic compounds results in greater competitive ability of invasive plants in the invaded range rather than in the native range.We also exam-ined the degree to which the allelopathy contributes increased com-petitive ability of S.canadensis in the invaded range.Methods We compared allelochemical production by S.canadensis growing in its native area(the USA)and invaded area(China)and also by populations that were collected from the two countries and grown together in a'common garden'greenhouse experiment.We also tested the allelopathic effects of S.canadensis collected from either the USA or China on the germination of Kummerowia striata(a native plant in China).Finally,we conducted a common garden,greenhouse experiment in which K.striata was grown in monocul-ture or with S.canadensis from the USA or China to test the effects of allopathy on plant-plant competition with suitable controls such as adding activated carbon to the soil to absorb the allelochemicals and thereby eliminating any corresponding allopathic effects.Important findings Allelochemical contents(total phenolics,total flavones and total saponins)and allelopathic effects were greater in S.canadensis sampled from China than those from the USA as demonstrated in a field survey and a common garden experiment.Inhibition of K.stri-ata germination using S.canadensis extracts or previously grown in soil was greater using samples from China than from the USA.The competitive ability of S.canadensis against K.striata was also greater for plants originating from China than those from the USA.Allelopathy could explain about 46%of the difference.These find-ings demonstrated that S.canadensis has evolved to be more allelo-pathic and competitive in the introduced range and that allelopathy significantly contributes to increased competitiveness for this inva-sive species. | Yongge Yuan Bing Wang Shanshan Zhang Jianjun Tang Cong Tu Shuijin Hu Jean W.H.Yong Xin Chen | 2013 | Journal of Plant Ecology2013，6，3： | 15 |
| 9 | Evaluation of contrast-enhanced ultrasound for diagnosis of dysplastic nodules with a focus of hepatocellular carcinoma in liver cirrhosis patients显示文摘Objective: To compare the enhancement features of dysplastic nodules with a focus of hepatocellular carcinoma(DN-HCC) versus HCC and regenerative nodules(RN) in cirrhotic patients.Methods: One hundred and ninety-three cirrhotic patients were enrolled in this study; they had 215 focal liver lesions, 1.0-3.5 cm in size, which were examined using contrast-enhanced ultrasound(CEUS) with Sono Vue?and diagnosed as HCC, RN or DN-HCC by biopsy. Samples were obtained using 18-gauge needles in the different enhanced areas. The enhancement features of DN-HCC, HCC and RN were evaluated.Results: There were 86 HCC lesions, 102 RN lesions, and 27 DN-HCC lesions diagnosed by biopsy. Of 86 HCC lesions, 87.2%(75/86) showed complete enhancement during the arterial phase, and 12.8%(11/86) had inhomogeneous enhancement, with no enhancement in the central area during the arterial phase; 100%(86/86) exhibited washout during the late phase. Of 102 RN lesions, 95.1%(97/102) had delayed or simultaneous enhancement during the arterial phase, and 4.9%(5/102) displayed slight enhancement during the arterial phase; 26.5%(27/102) exhibited washout and 73.5%(75/102) exhibited no washout during the late phase. In 27 DN-HCC lesions, only part of the lesions enhanced during the arterial phase and washed out during the late phase; the other areas had delayed or simultaneous enhancement during the arterial phase, and 29.6%(8/27) exhibited slight washout in the late phase. In 86 HCCs, the pathological feature was HCC in the enhanced area of 75 lesions, hepatocellular fatty degeneration in the slightly enhanced area of 7 lesions, and hepatocellular necrosis in the unenhanced area and HCC in the enhanced area of 4 lesions. In 102 RNs, the pathological diagnosis was hepatocyte proliferation with or without fatty degeneration. In 27 DN-HCCs, the pathological feature was HCC in the enhanced area and hepatocyte regeneration in the unenhanced area.Conclusions: CEUS is useful for the diagnosis of focal liver lesions in cirrhotic patients. CEUS can help determine the progression from RN to DN-HCC to HCC by analyzing the hemodynamics. CEUS can promote the diagnostic accuracy of a biopsy by providing more accurate information on the site of the biopsy. | Wei Wu Minhua Chen Kun Yan Yin Dai Shanshan Yin Wei Yang Zhihui Fan | 2015 | Chinese Journal of Cancer Research2015，27，1： | 15 |
| 10 | The prognostic and therapeutic implications of circulating tumor cell phenotype detection based on epithelial-mesenchymal transition markers in the first-line chemotherapy of HER2-negative metastatic breast cancer显示文摘Background:Epithelial-mesenchymal transition(EMT)is implicated in the metastatic process and presents a chal-lenge to epithelial cell adhesion molecule-based detection of circulating tumor cells(CTCs),which have been demon-strated to be a prognostic indicator in metastatic breast cancer.Although evidence has indicated that heterogeneity of CTCs based on EMT markers is associated with disease progression,no standard recommendations have been established for clinical practice.This study aimed to evaluate the prognostic significance of dynamic CTC detection based on EMT for metastatic breast cancer patients.Methods:We enrolled 108 human epidermal growth factor receptor 2-negative metastatic breast cancer patients from the prospective phase III CAMELLIA study and applied the CanPatrol CTC enrichment technique to identify CTC phenotypes(including epithelial CTCs,biphenotypic epithelial/mesenchymal CTCs,and mesenchymal CTCs)in peripheral blood samples.Receiver operating characteristic curve analyses of total CTC count and the proportion of mesenchymal CTCs for predicting the 1-year progression-free survival(PFS)rate were conducted to determine the optimal cut-off values,and Kaplan-Meier analysis and Cox proportional hazards regression analysis were performed to investigate the prognostic value of the cut-off values of both total CTC count and the proportion of mesenchymal CTCs in combination.Results:For predicting the 1-year PFS rate,the optimal cut-off value of total CTC count was 9.5(Area under the curve[AUC]=0.538,95%confidence interval[CI]=0.418-0.657),and that of the proportion of mesenchymal CTCs was 10.7%(AUC=0.581,95%CI=0.463-0.699).We used the two cut-off values in combination to forecast PFS in which the total CTC count was equaled to or exceeded 10/5 mL with the proportion of mesenchymal CTCs surpassed 10.7%.Patients who met the combined criteria had significantly shorter median PFS than did those who did not meet the criteria(6.2 vs.9.9 months,P=0.010).A nomogram was constructed based on the criteria and significant clinicopatho-logical characteristics with a C-index of 0.613(P=0.010).Conclusions: The criteria, which combine the total CTC count and the proportion of mesenchymal CTCs, may be used to monitor therapeutic resistance and predict prognosis in patients with metastatic breast cancer. | Xiuwen Guan Fei Ma Chunxiao Li Shiyang Wu Shangying Hu Jiefen Huang Xiaoying Sun Jiayu Wang Yang Luo Ruigang Cai Ying Fan Qiao Li Shanshan Chen Pin Zhang Qing Li Binghe Xu | 2019 | Cancer Communications2019，39，1： | 15 |
| 11 | A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice显示文摘The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI).However,the calcium channels responsible for this process have remained unknown.Here,we show that rice CDSI(CELL DEATH and SUSCEPTIBLE to BLAST 1)encoding OsCNGC9;a cyclic nucleotide-gated channel protein,positively regulates the resistance to rice blast disease.We show that OsCNGC9 mediates PAMP-induced Ca2+influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression.We further show that a PTI related receptor-lih cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity.Our results suggest a signaling cascade linking pattern recognition to calcium channel activation,which is required for initiation of PTI and disease resistance in rice. | Jiachang Wang Xi Liu An Zhang Yulong Ren Fuqing Wu Gang Wang Yang Xu Cailin Lei Shanshan Zhu Tian Pan Yongfei Wang Huan Zhang Fan Wang Yan-Qiu Tan Yupeng Wang Xin Jin Sheng Luo Chunlei Zhou Xiao Zhang Jinling Liu Shuai Wang Lingzhi Meng Yihua Wang Xi Chen Qibing Lin Xin Zhang Xiuping Guo Zhijun Cheng Jiulin Wang Yunlu Tian Shijia Liu Ling Jiang Chuanyin Wu Ertao Wang Jian-Min Zhou Yong-Fei Wang Haiyang Wang Jianmin Wan | 2019 | Cell Research2019，29，10： | 13 |
| 12 | Effect of contrast-enhanced ultrasound on differential diagnosis of intrahepatic cholangiocarcinoma and arterial phase enhanced hepatic inflammatory lesions显示文摘Objective: To investigate differential diagnosis between intrahepatic cholangiocarcinoma(ICC) and arterial phase enhanced hepatic inflammatory lesions in patients without liver cirrhosis using contrast-enhanced ultrasound(CEUS).Methods: ICC and hepatic inflammatory lesions cases with CEUS and pathological diagnosis between Sep 2013 and Oct 2016 were investigated retrospectively. Imaging features of conventional ultrasound and CEUS were analyzed. The parameters of time intensity curve(TIC), including the arrival time, peak intensity(PI) in the lesions, the starting time for washout, and the intensity difference at 3 min(ΔI_3) after contrast agent infection between the lesion and the liver parenchyma, were compared between ICC and hepatic inflammatory lesions.Results: Twenty-five ICC and fifteen inflammatory patients were included in this study. Seventeen ICC(68.0%)and two inflammatory cases(13.3%) showed bile duct dilatation on conventional ultrasound. Using CEUS, three ICC cases(12.0%) were misdiagnosed as inflammatory lesions and three inflammatory lesions(20.0%) as ICC; two ICC(8.0%) and one inflammatory case(6.7%) could not be made definite diagnosis. Washout started at 34.5±3.5 s and 61.5±12.9 s for ICC and inflammatory lesions respectively(P<0.001). The intensity difference between lesion and liver parenchyma at 3 min after contrast agent injection was 10.8±3.1 dB in ICC and 4.2±2.3 dB in inflammatory group(P<0.001). The sensitivity and specificity differentiating ICC and inflammatory lesions were76% and 87% if the cut-off value of the intensity difference was 7.7 d B.Conclusions: Combined with TIC analysis, and particularly with the characteristic of the early-starting and obvious washout in ICC, CEUS can be useful in differential diagnosis between hepatic inflammatory lesions and ICC. | Shanshan Yin Qiuli Cui Kun Yan Wei Yang Wei Wu Liping Bao Minhua Chen | 2017 | Chinese Journal of Cancer Research2017，29，3： | 13 |
| 13 | Glacier retreat and its effect on stream flow in the source region of the Yangtze River显示文摘在冰河上在气候变化的效果上正在种担心融化并且水文学。在这篇文章,我们在长江的来源区域使用了二自然的小规模的盆, Tuotuo 河和 Buqu 河,中国,到表演,冰河的影响融化在溪流流动上。在在 1970, 1992 和 2009 的冰河和冰体积的程度的变化用遥感图象被评估。在一样的时间间隔上的冰河表面区域的变化用 Landsat TM/ETM+ 形象通过冰河轮廓和位置的描述被估计。在 2009,冰河表面区域分别地在 Tuotuo 河和 Baqu 河盆由 1970 价值中的 20.83% 个和 34.81% 减少了。在每盆的全部的 meltwater 供应被估计是 2.56 ?? | WU Shanshan YAO Zhijun HUANG Heqing LIU Zhaofei CHEN Yuansheng | 2013 | Journal of Geographical Sciences2013，23，5： | 13 |
| 14 | Factors Associated with Thrombolysis Outcome in Ischemic Stroke Patients with Atrial Fibrillation显示文摘The outcome of early intravenous thrombolysis for ischemic stroke in patients with atrial fibrillation(AF)is worse than that without thrombosis. How to increase the efficacy of intravenous thrombolysis for AF-related ischemic stroke remains largely unknown. In this study, we investigated factors that influence the effect of intravenous thrombolysis in these patients. Our results showed that thrombolysis was independently associated with a favorable outcome(P \ 0.001) and did not influence the mortality of AF-related ischemic stroke, although it increased the risk of hemorrhage within 24 h after treatment. Risk factors for a poor outcome at admission were:heart failure(P = 0.045); high systolic pressure(P = 0.039); high blood glucose(P = 0.030); and a high National Institutes of Health Stroke Scale(NIHSS) score(P \ 0.001). Moreover, high systolic pressure at admission(P = 0.007), high blood glucose(P = 0.027), and a high NIHSS score(P \ 0.001) were independent risk factors for mortality at 3 months. Besides thrombolysis, a high NIHSS score(P = 0.006) and warfarin taken within 48 h before stroke onset(P = 0.032) were also independent risk factors for symptomatic hemorrhage within 24 h after treatment. Ischemic stroke patients with AF benefited from intravenous thrombolysis with recombinant tissue plasminogen activator within 4.5 h after stroke. | Qiuyun Zhao Xiaobo Li Wanli Dong Min Ye Yongjun Cao Meijuan Zhang Qiantao Cheng Junshan Zhou Guofang Chen Ming Yu Shanshan Hong Xiue Wei Bei Wang Guiyun Cui Peng Zhang Hong Ding Rongzhen Xu Yan Chen Yun Xu | 2016 | Neuroscience Bulletin2016，32，2： | 13 |
| 15 | Hypocrellin A-based photodynamic action induces apoptosis in A549 cells through ROS-mediated mitochondrial signaling pathway显示文摘Over recent decades, many studies have reported that hypocrellin A(HA) can eliminate cancer cells with proper irradiation in several cancer cell lines. However, the precise molecular mechanism underlying its anticancer effect has not been fully defined. HA-mediated cytotoxicity and apoptosis in human lung adenocarcinoma A549 cells were evaluated after photodynamic therapy(PDT). A temporal quantitative proteomics approach by isobaric tag for relative and absolute quantitation(iTRAQ) 2 D liquid chromatography with tandem mass spectrometric(LC–MS/MS) was introduced to help clarify molecular cytotoxic mechanisms and identify candidate targets of HA-induced apoptotic cell death. Specific caspaseinhibitors were used to further elucidate the molecular pathway underlying apoptosis in PDT-treated A549 cells. Finally, down-stream apoptosis-related protein was evaluated. Apoptosis induced by HA was associated with cell shrinkage, externalization of cell membrane phosphatidylserine, DNA fragmentation,and mitochondrial disruption, which were preceded by increased intracellular reactive oxygen species(ROS) generations. Further studies showed that PDT treatment with 0.08 mmol/L HA resulted in mitochondrial disruption, pronounced release of cytochrome c, and activation of caspase-3,-9, and-7.Together, HA may be a possible therapeutic agent directed toward mitochondria and a promising photodynamic anticancer candidate for further evaluation. | Shanshan Qi Lingyuan Guo Shuzhen Yan Robert J.Lee Shuqin Yu Shuanglin Chen | 2019 | Acta Pharmaceutica Sinica B2019，9，2： | 12 |
| 16 | Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods显示文摘一个金 immunochromatographic 传感器(GICS ) 在蜂蜜样品为 26 硫安类药剂的快速的察觉被开发。传感器基于能认出所有 26 硫安类药剂的组特定的 monoclonal 抗体(mAb ) 。三 haptens (1 与 thiazole 包围的 hapten, 2 与苯包围的 hapten,和 3 与直碳锁住的 hapten ) 被用于抗原准备。与 hybridoma 技术,组特定的 mAb 与 50% 最大的禁止的集中被屏蔽(对 sulfathizole (STZ ) 和从 0.08 ~ 90.18 ng/mL 的另外的 25 类似物的 IC 50) 。单音分散金 nanoparticles 与 mAb 被结合开发侧面的 immunochromatographic 长带。0.1 g/mL 的标记的抗体集中和在测试线的 0.2 g/mL 的涂层抗原集中为长带准备被选择。在优化条件,为 STZ 的集中的察觉( vLOD )的视觉限制, sulfamethoxazole , sulfamethizole ,磺胺嘧啶, sulfamerazine , sulfadimethoxine , sulfamonomethoxine , sulfameter , sulfamethoxypyridazine ,和 sulfachloropyridazine 下面是 5 , 0.25 , 0.25 , 10 , 5 , 10 , 25 , 2.5 , 5 , 0.25 ,和 10 g/kg 分别地。在蜂蜜样品的扫描仪分析为 26 硫安类药剂的察觉揭示了好性能。商业蜂蜜样品与传感器被测试,积极结果与高效的液体层析被证实。建议脱衣传感器在爱人样品为硫安类药剂污染物质的快速、可靠的决心提供一个方便方法。 | Yanni Chen Liqiang Liu Liguang Xu Shanshan Song Hua Kuang Gang Cui Chuanlai Xu | 2017 | Nano Research2017，10，8： | 11 |
| 17 | Sleep Deprivation Selectively Down-Regulates Astrocytic 5-HT2B Receptors and Triggers Depressive-Like Behaviors via Stimulating P2X7 Receptors in Mice显示文摘Chronic loss of sleep damages health and disturbs the quality of life.Long-lasting sleep deprivation(SD)as well as sleep abnormalities are substantial risk factors for major depressive disorder,although the underlying mechanisms are not clear.Here,we showed that chronic SD in mice promotes a gradual elevation of extracellular ATP,which activates astroglial P2X7 receptors(P2X7Rs).Activated P2X7Rs,in turn,selectively down-regulated the expression of 5-HT2B receptors(5-HT2BRs)in astrocytes.Stimulation of P2X7Rs induced by SD selectively suppressed the phosphorylation of AKT and FoxO3 a in astrocytes,but not in neurons.The overexpression of FoxO3a in astrocytes inhibited the expression of 5-HT2BRs.Down-regulation of 5-HT2BsRs instigated by SD suppressed the activation of STAT3 and relieved the inhibition of Ca2+-dependent phospholipase A2.This latter cascade promoted the release of arachidonic acid and prostaglandin E2.The depression-like behaviors induced by SD were alleviated in P2X7R-KO mice.Our study reveals the mechanism underlying chronic SD-induced depression-like behaviors and suggests 5-HT2BRs as a key target for exploring therapeutic strategies aimed at the depression evoked by sleep disorders. | Maosheng Xia Zexiong Li Shuai Li Shanshan Liang Xiaowei Li Beina Chen Manman Zhang Chengyi Dong Alexei Verkhratsky Dawei Guan Baoman Li | 2020 | Neuroscience Bulletin2020，36，11： | 10 |
| 18 | ADAR1 is required for differentiation and neural induction by regulating microRNA processing in a catalytically independent manner显示文摘对 RNA (ADAR ) 起作用的腺苷脱氨基酶涉及编辑的 adenosine-to-inosine RNA 并且在开发和疾病被含有。这里,我们观察到在人的胚胎的干细胞(hESCs ) 的那 ADAR1 缺乏显著地在 mRNA 和 miRNA 表示与普遍变化影响了 hESC 区别和神经正式就职,包括 self-renewal-related miRNAs 的 upregulation,例如 miR302s。全球编辑分析表明编辑活动的 ADAR1 在神经正式就职之上在 ADAR1 缺乏的 hESCs 贡献很少到改变的 miRNA/mRNA 表示。染色体宽的 iCLIP 研究鉴别 ADAR1 直接绑在 pri-miRNAs 防碍 miRNA 由充当 RNA 有约束力的蛋白质处理。重要地,在神经区别之上在弄空 ADAR1 的 hESCs 观察的 miRNAs 和显型的异常表示能被酶地不活跃的 ADAR1 异种,然而并非由 RNA-binding-null ADAR1 异种颠倒。这些调查结果揭示那 ADAR1,然而并非它的编辑活动,由经由直接 RNA 调整 miRNA 生物的续生说为 hESC 区别和神经正式就职是批评的相互作用。 | Tian Chen Jian-Feng Xiang Shanshan Zhu Siye Chen Qing-Fei Yin Xiao-Ou Zhang Jun Zhang Hua Feng Rui Dong Xue-Jun Li Li Yang Ling-Ling Chen | 2015 | Cell Research2015，25，4： | 10 |
| 19 | Development and validation of an endoscopic images-based deep learning model for detection with nasopharyngeal malignancies显示文摘Background:Due to the occult anatomic location of the nasopharynx and frequent presence of adenoid hyperpla-sia,the positive rate for malignancy identification during biopsy is low,thus leading to delayed or missed diagnosis for nasopharyngeal malignancies upon initial attempt.Here,we aimed to develop an artificial intelligence tool to detect nasopharyngeal malignancies under endoscopic examination based on deep learning.Methods:An endoscopic images-based nasopharyngeal malignancy detection model(eNPM-DM)consisting of a fully convolutional network based on the inception architecture was developed and fine-tuned using separate training and validation sets for both classification and segmentation.Briefly,a total of 28,966 qualified images were collected.Among these images,27,536 biopsy-proven images from 7951 individuals obtained from January 1st,2008,to December 31st,2016,were split into the training,validation and test sets at a ratio of 7:1:2 using simple randomiza-tion.Additionally,1430 images obtained from January 1st,2017,to March 31st,2017,were used as a prospective test set to compare the performance of the established model against oncologist evaluation.The dice similarity coef-ficient(DSC)was used to evaluate the efficiency of eNPM-DM in automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images,by comparing automatic segmentation with manual segmenta-tion performed by the experts.Results:All images were histopathologically confirmed,and included 5713(19.7%)normal control,19,107(66.0%)nasopharyngeal carcinoma(NPC),335(1.2%)NPC and 3811(13.2%)benign diseases.The eNPM-DM attained an overall accuracy of 88.7%(95%confidence interval(CI)87.8%-89.5%)in detecting malignancies in the test set.In the prospective comparison phase,eNPM-DM outperformed the experts:the overall accuracy was 88.0%(95%CI 86.1%-89.6%)vs.80.5%(95%CI 77.0%-84.0%).The eNPM-DM required less time(40 s vs.110.0±5.8 min)and exhibited encouraging performance in automatic segmentation of nasopharyngeal malignant area from the background,with an average DSC of 0.78±0.24 and 0.75±0.26 in the test and prospective test sets,respectively.Conclusions:The eNPM-DM outperformed oncologist evaluation in diagnostic classification of nasopharyngeal mass into benign versus malignant,and realized automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images. | Chaofeng Li Bingzhong Jing Liangru Ke Bin Li Weixiong Xia Caisheng He Chaonan Qian Chong Zhao Haiqiang Mai Mingyuan Chen Kajia Cao Haoyuan Mo Ling Guo Qiuyan Chen Linquan Tang Wenze Qiu Yahui Yu Hu Liang Xinjun Huang Guoying Liu Wangzhong Li Lin Wang Rui Sun Xiong Zou Shanshan Guo Peiyu Huang Donghua Luo Fang Qiu Yishan Wu Yijun Hua Kuiyuan Liu Shuhui Lv Jingjing Miao Yanqun Xiang Ying Sun Xiang Guo Xing Lv | 2018 | Cancer Communications2018，38，1： | 9 |
| 20 | Acupuncture at Waiguan (TE5) influences activation/deactivation of functional brain areas in ischemic stroke patients and healthy people A functional MRI study显示文摘In the present study, 10 patients with ischemic stroke in the left hemisphere and six healthy controls were subjected to acupuncture at right Waiguan (TE5). In ischemic stroke subjects, functional MRI showed enhanced activation in Broadmann areas 5, 6, 7, 18, 19, 24, 32, the hypothalamic inferior lobe, the mamillary body, and the ventral posterolateral nucleus of the left hemisphere, and Broadmann areas 4, 6, 7, 18, 19 and 32 of the right hemisphere, but attenuated activation of Broadmann area 13, the hypothalamic inferior lobe, the posterior lobe of the tonsil of cerebellum, and the culmen of the anterior lobe of hypophysis, in the left hemisphere and Broadmann area 13 in the right hemisphere. In ischemic stroke subjects, a number of deactivated brain areas were enhanced, including Broadmann areas 6, 11, 20, 22, 37, and 47, the culmen of the anterior lobe of hypophysis, alae lingulae cerebella, and the posterior lobe of the tonsil of cerebellum of the left hemisphere, and Broadmann areas 8, 37, 45 and 47, the culmen of the anterior lobe of hypophysis, pars tuberalis adenohypophyseos, inferior border of lentiform nucleus, lateral globus pallidus, inferior temporal gyrus, and the parahippocampal gyrus of the right hemisphere. These subjects also exhibited attenuation of a number of deactivated brain areas, including Broadmann area 7. These data suggest that acupuncture at Waiguan specifically alters brain function in regions associated with sensation, vision, and motion in ischemic stroke patients. By contrast, in normal individuals, acupuncture at Waiguan generally activates brain areas associated with insomnia and other functions. | Junqi Chen Yong Huang Xinsheng Lai Chunzhi Tang Junjun Yang Hua Chen Tongjun Zeng Junxian Wu Shanshan Qu | 2013 | Neural Regeneration Research2013，8，3： | 8 |
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