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| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | Growth mechanism and growth habit of oxide crystals显示文摘 | LI Wenjun SHI Erwei ZHONG Weizhuo | 1999 | Journal of Crystal Growth1999，203，1： | 1 |
| 3 | Hydrothermal preparation and characterization of brookite-type TiO2 nanocrystallites显示文摘 | ZHENG YANGQING SHI ERWEI CUI SUXIAM | 2000 | J Mater Sci lett2000，，19： | 1 |
| 4 | Growth Mechanism and Growth Habit of Oxide Crystals显示文摘 | Li Wenjun Shi Erwei Zhong Weizhuo | 1999 | Jour nal of Crystal Growth1999，203，18： | 1 |
| 5 | Growth Habit of Rutile and a-Alz O3 Crystals显示文摘 | Li Wenjun Shi Erwei Yin Zhiwen | 2000 | Journal of Crystal Growth2000，208，13： | 1 |
| 6 | Synthesis of mono-dispersed ZnA1204 powders under hydrothermal conditions 显示文摘 | Chen Z Z Shi Erwei Zheng Yanqing | 2002 | Materials Letters2002，56，： | 1 |
| 7 | Growth Mechanism and Growth Habit of Oxide Crystals显示文摘 | Li Wenjun Shi Erwei Zhong Weizhuo | 1999 | J Cryst Growth1999，203，12： | 1 |
| 8 | Growth of hex-pod-like Cu2O whisker under hydrothermal conditions 显示文摘 | Chen Zhizhan Shi Erwei Zheng Yanqing | 2003 | Journal of Crystal Growth2003，249，12： | 1 |
| 9 | Influence of solution concentration on the hydrothermal preparation of titania crystallites显示文摘 | Zheng Yanqing Shi Erwei Chen Zhizhan | 2001 | Journal of Material Chemistry2001，11，5： | 1 |
| 10 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 11 | Hydrothermal synthesis and optical property of nano-sized CoAl2O4pigment显示文摘 | Zhizhan Chen Erwei Shi Wenjun Li | 2002 | Materials Letters2002，55，： | 1 |
| 12 | Infuence of solution concentration on the hydrothermal preparation of titania crystallites显示文摘 | Zheng Yanqing Shi Erwei Chen Zhizhan | 2000 | J Mater Chem2000，11，： | 1 |
| 13 | Hydrothermal preparation and characterization of brookite-type TiO2 nanocrystallites显示文摘 | Zheng Yangqing Shi Erwei Cui Suxiam | 2000 | J Mater Sci Lett2000，16，19： | 1 |
| 14 | Hydrothermal synthesis and optical property of nano--siaed CoM a 04 pig- ment显示文摘 | Chen Zhizhan Shi Erwei Li Wenjun | 2002 | Mater Lett2002，55，： | 1 |
| 15 | Hydrothermal synthesis and optical property of nanosizod COAl2O4 pigment显示文摘 | Chen Zhizhan Shi Erwei Li Ywenjun | | 0，，05： | 1 |
| 16 | Hydrothermal synthesis and optical property of nano-sized CoA12O4 pigment显示文摘 | SHI Erwei LI Wenjun | 2002 | Mater Lett2002，55，： | 1 |
| 17 | Hydrothermal synthesis and optical property of nano-sized CoAl2 O4 pigment显示文摘 | Chen Zhizhan Shi Erwei Li Wenjun | 2002 | Mater Lett2002，55，： | 1 |
| 18 | Morphological characteristics of α-Al_2O_3 crystallites obtained directly from hydro/solvothermal solvents显示文摘The morphological characteristics of α_Al-2O-3 crystallites obtained directly from hydro/solv othermal solvents are reported and the formation mechanisms of corundum morphology are discussed from crystal growth and crystal chemistry principles. The crystal growth process is considered as a process of incorporation of growth units on the growth interfaces, and the crystal morphology is determined by the linkage of the coordinated polyhedra. | WANG Buguo, TIAN Mingyuan, SHI Erwei, ZHONG Weizhuoand GUO Jingkun Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 201800, China | 1998 | Chinese Science Bulletin1998，43，2： | 1 |
| 19 | 显示文摘 | Xia Changtai Shi Erwei Zhong Weizhuo | 1996 | J Cryst Growth1996，166，： | 1 |
| 20 | Preparation of nanosized cobalt aluminate powders by a hydrothermal method显示文摘 | CHEN Zhizhan SHI Erwei ZHENG Yanqing | 2004 | Mater Sci and Eng B2004，107，2： | 1 |
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