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| 1 | MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1显示文摘糖尿病和许多另外的新陈代谢症候群是仔细与肥胖有关。揭示胖免职的内在的机制,越来越多的研究在 adipocytes 开发期间正在集中于 miRNAs 的功能。以前的研究 haveproved 那出 miR-15a/b 戏在多重生理的过程的重要角色;然而,他们的功能在 adipogenesisremain 期间不清楚。揭示这,我们在猪的 pre-adipocyte 在 adipogenesis 期间检测了 miR-15a/b 的表示侧面,并且发现那他们层次在 adipocyte 区别的早阶段增加了并且在白天 4 .Moreover 以后落下的表示,在猪的 pre-adipocytes 的 miR-15a/b 的在表示上支持了 adipocyte 区别, miR-15a/b 的类脂化合物 accumulation.Target 基因被预言并且检验,它表明 Forkhead 盒子蛋白质 O1 ( FoxO1 )是目标 geneof miR-15a/b 。表示水平由 miR-15a/b 在表示上引起了的 FoxO1 的抑制在 adipogenesis.Thus 上有积极效果,我们断定 miR-15a/b 经由镇压在猪的 pre-adipocyte 支持 adipogenesis FoxO1。 | Peiyue Dong Yin Mai Zhenyu Zhang Lin Mi Guofang Wu Guiyan Chu Gongshe Yang Shiduo Sun | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 6 |
| 2 | Differential expression of meat quality and intramuscular fat deposition related genes in Hanjiang black pigs显示文摘 | Guofang Wu Xin'e Shi Jiping Zhou Lei Wang Yuefeng Li Chengchuang Song Aoqi Xiang Peiyue Dong Shiduo Sun | 2014 | Acta Biochimica et Biophysica Sinica2014，46，12： | 3 |
| 3 | Sirt2 induces C2C12 myoblasts proliferation by activation of the ERK1/2 pathway显示文摘 | Guofang Wu Chengchuang Song Hongzhao Lu Long Jia Gongshe Yang Xin'e Shi Shiduo Sun | 2014 | Acta Biochimica et Biophysica Sinica2014，46，4： | 2 |
| 4 | Elevated miR-10a-5p facilitates cell cycle and restrains adipogenic differentiation via targeting Map2k6 and Fasn,respectively显示文摘miRNAs are a small class of noncoding RNAs that perform biological functions by regulating the stability or translation of target genes in various biological processes.This study illustrated the role of miR-10a-5p,which is relatively enriched in adipose tissues,using primary mouse preadipocytes as model.With elevated miR-10a-5p expression,the proliferative ability of mouse preadipocytes was significantly enhanced,indicated by increased EdU+cells and G1/S transition,accompanied by upregulated Cyclin B,Cyclin D and PCNA and downregulated p21 and p27.Meanwhile,the adi-pogenic differentiation was significantly attenuated by elevated miR-10a-5p,supported by Oil Red 0 staining and suppressed PPARγand aP2 expression.Furthermore,Map2k6 and Fasn were pre-dicted to be the target genes of miR-10a-5p in silico,and dual luciferase reporter assay confirmed the direct targeting effects.Western blot analysis results showed that miR-10a-5p specially reduced Map2k6 expression at the proliferative stage without affecting Fasn expression,while significantly restrained Fasn expression with unchanged Map2k6 expression during adipogenic differentiation.Taken together,these results revealed a potential role of miR-10a-5p in adipogenesis and in the treatment of obesity. | Xiaoyu Wang Huifang Zhang Meixue Xu Xin'E Shi Gongshe Yang Shiduo Sun Xiao Li | 2020 | Acta Biochimica et Biophysica Sinica2020，52，11： | 2 |
| 5 | Over-expression of miR-125a-5p inhibits proliferation in C2C12 myoblasts by targeting E2F3显示文摘MicroRNAs miRNAs 是在长度的 2025 核苷酸的小非编码的 RNA 的一个班。miRNAs 在房间的许多类型的增长起重要作用,这被显示出,包括 myoblasts。在这研究,我们使用了即时量的聚合酶链反应,西方的弄污,在 C2C12 myoblasts 的增长期间探索 miR-125a-5p 的角色的 EdU,流动 cytometry,和 CCK-8 试金。miR-125a-5p 的表示在 C2C12 myoblast 增长期间被减少,这被发现。miR-125a-5p 的在表示上禁止了由 EdU 染色,流动 cytometry,和 CCK8 试金显示了的 C2C12 myoblast 增长。miR-125a-5p 能否定地在 posttranscriptional 水平调整 E2F3 表示,这也被发现,经由在 3 untranslated 区域的一个特定的目标地点。显示出的 E2F3 击倒 C2C12 myoblast 增长上的类似的禁止的效果。因此,我们的调查结果建议 miR-125a-5p 可以由指向 E2F3 充当 C2C12 myoblast 增长的一个否定管理者。 | Chengchuang Song Guofang Wu Aoqi Xiang Qiangling Zhang Wanhua Li Gongshe Yang Xin'e Shi Shiduo Sun Xiao Li | 2015 | Acta Biochimica et Biophysica Sinica2015，47，4： | 1 |
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