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| 1 | Comprehensive and innovative techniques for livertransplantation in rats: A surgical guide显示文摘AIM: To investigate our learning curves of orthotopic liver transplantation (OLT) in rats and the most important factor for successful surgery. METHODS: We describe the surgical procedures for our rat OLT model, and determined the operator learning curves. The various factors that contributed to successful surgery were determined. The most important surgical factors were evaluated between successful and unsuccessful surgeries.RESULTS: Learning curve data indicated that 50 cases were required for operator training to start a study. Operative time, blood loss, warm ischemic time, anhepatic phase, unstable systemic hemodynamic state, and body temperature after surgery significantly affected surgery success by univariate analysis, while the anhepatic phase was the most critical factor for success by multivariate analysis. CONCLUSION: OLT in rats is the only liver transplantation model that provides clinically relevant and reliable results. Shortened anhepatic phase is key to success in this model. | Tomohide Hori Justin H Nguyen Yasuhiro Ogura Toshiyuki Hata Shintaro Yagi Ann-Marie T Baine Norifumi Ohashi Christopher B Eckman Aimee R Herdt Hiroto Egawa Yasutsugu Takada Fumitaka Oike Seisuke Saka-moto Mureo Kasahara Kohei Ogawa Koichiro Hata Taku Iida Yukihide Yonekawa Lena Sibulesky Kagemasa Kuribayashi Takuma Kato Kanako Saito Mie Torii Naruhiko Sahara Naoko Kamo Tomoko Sahara Motohiko Yasutomi Shinji Uemoto | 2010 | World Journal of Gastroenterology2010，16，25： | 14 |
| 2 | Oxidative stress and extracellular matrices after hepatectomy and liver transplantation in rats显示文摘AIM:To investigate oxidative stress(OS)-mediated damage and the behavior of extracellular matrices in various rat models because shear stress with portal hypertension and cold ischemia/warm reperfusion injury trigger the liver regeneration cascade after surgery. These injuries also cause fatal liver damage.METHODS: Rats were divided into four groups according to the surgery performed: control; hepatectomy with 40% liver remnant(60% hepatectomy); orthotopic liver transplantation(OLT) with whole liver graft(100% OLT); and split OLT(SOLT) with 40% graft(40% SOLT). Survival was evaluated. Blood and liver samples were collected at 6 h after surgery. Biochemical and histopathological examinations were performed. OSinduced damage, 4-hydroxynonenal, ataxia-telangiectasia mutated kinase, histone H2AX, phosphatidylinositol 3-kinase(PI3K) and Akt were evaluated by western blotting. Behavior of extracellular matrices, matrix metalloproteinase(MMP)-9, MMP-2, tissue inhibitor of metalloproteinase(TIMP)-1 and TIMP-2 were also evaluated by western blotting and zymography. RESULTS: Although 100% OLT survived, 60% hepatectomy and 40% SOLT showed poor survival. Histopathological, immunohistological, biochemical and protein assays revealed that 60% hepatectomy, 100% OLT and 40% SOLT showed liver damage. PI3K and Akt were decreased in 60% hepatectomy and 40% SOLT. For protein expression, 40% SOLT showed differences in MMP-9, MMP-2 and TIMP-2. TIMP-1 showed differences in 60% hepatectomy and 40% SOLT. For protein activity, MMP-9 demonstrated significant differences in 60% hepatectomy, 100% OLT and 40% SOLT. CONCLUSION: Under conditions with an insufficient liver remnant, prevention of OS-induced damage via the Akt/PI3K pathway may be key to improve the postoperative course. MMP-9 may be also a therapeutic target after surgery. | Tomohide Hori Shinji Uemoto Feng Chen Lindsay B Gardner Ann-Marie T Baine Toshiyuki Hata Takayuki Kogure Justin H Nguyen | 2014 | World Journal of Hepatology2014，6，2： | 7 |
| 3 | Simple and reproducible hepatectomy in the mouse using the clip technique显示文摘AIM: To investigate the reliability of massive hepatectomy models by using clip techniques. METHODS: We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction > 70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves, and histopathological findings.RESULTS: According to anatomical results, models with 75%, 80%, and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques. CONCLUSION: Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data. | Tomohide Hori Norifumi Ohashi Feng Chen Ann-Marie T Baine Lindsay B Gardner Toshiyuki Hata Shinji Uemoto Justin H Nguyen | 2012 | World Journal of Gastroenterology2012，18，22： | 4 |
| 4 | Design of synthesis of phthalimide-type histone deacelase inhibitors 显示文摘 | Shinji C Nakamura T Maseda S | 2005 | Bioorg &Med Chem Lett2005，15，： | 1 |
| 5 | Measles virus: cellular recep- tors, tropism and pathogenesis 显示文摘 | Yusuke Y Makoto T Shinji O | 2006 | J Gen Virol2006，87，： | 1 |
| 6 | Combined analysis of polymorphisms in angiotensinogen and adduein genes and their effects on hypertension in a Japanese sample: The shigaraki study 显示文摘 | Shinji T Yasuyuki N Yasuharu T | 2005 | Hypertension Res2005，28，8： | 1 |
| 7 | Inner structure of potato starch gra nules显示文摘 | Shinji T Katsunori T Makoto H Tetsuya Y | 1997 | Starch/Strke1997，49，： | 1 |
| 8 | Expression of hypoxia-inducible factor-1α is associated with tumor vascularization in human colorectal carcinoma显示文摘 | Kawai T Yasuhiko K Shinji T | 2003 | Int J Cancer2003，150，： | 1 |
| 9 | Electrical conductivity of proton conductive ceramics under reactor irradiation 显示文摘 | Tatsuo S Bun T Shinji N | 2006 | Advances in Science and Technology2006，45，： | 1 |
| 10 | Clinical characteristics of young-onset isehemic colitis 显示文摘 | kimura T Shinji A Horirchi A | 2012 | Dig Dis Sci2012，57，6： | 1 |
| 11 | Calcitonin gene-related peptide,substance P,and tyrosine hydroxylase-immunoreactive innervation of rat bone marrows: An immunohistochemical and ultrastructural investigation on possible efferent and afferent mechanisms 显示文摘 | Shinji I Yoshimitsu T Toshihiro M | 1997 | J Orthop Res1997，15，： | 1 |
| 12 | Clinical and biological significance of lymph node micrometastases of colorectal cancer显示文摘 | Shinji S Naito Z Ishiwata T | 2007 | J Nippon Med Sch2007，74，1： | 1 |
| 13 | Hypocarbia and cystic periventricular leukomalacia in premature infants 显示文摘 | Shinji F Hajime T Nobuyuki Y | 1994 | Arch Dis Childhood1994，71，： | 1 |
| 14 | Cutaneous tissue angiotensin-converting enzyme may participate in pathologic scar formation in human skin 显示文摘 | Kiyoshi M Shinji T Hideya T | 2006 | Am Acad Dermatol2006，54，2： | 1 |
| 15 | Pulmonary and Sys temic Distribution of Inhaled Ultrafine Silver Particles in Rats 显示文摘 | Shinji T grwin K Christa R | 2001 | Environmental Health Perspectives2001，109，4： | 1 |
| 16 | Water- soluble extracts from Angelica acutiloba kitagawa enhance hematopoiesis by activating immature erythroid cells in mice with 5 - fluorouracil - induced anemia 显示文摘 | Ryo H Fumihide T Shinji F | 2004 | Experimental Hematology2004，32，： | 1 |
| 17 | Double-ballon enteroscopy inpatients with GI bleeding of obscure origin 显示文摘 | Noriaki M Shinji T Akira F | 2006 | Gastrointest Endose2006，64，1： | 1 |
| 18 | Rheological properties of reversible thermo-setting in situ gelling solutions with the methylcellulose-polyethylene glycol- citric acid ternary system 显示文摘 | Masanobu T Shinji K Hidekazu S | 2003 | Colloid Polym Sci2003，281，： | 1 |
| 19 | Staphylococcus epidermidis Esp inhibits Staphylococcus aureus biofilm formation and nasal colonization 显示文摘 | Iwase T Uehara Y Shinji H Tajima A Seo H Takada K Agata T Mizunoe Y | 2010 | Nature2010，465，7296： | 1 |
| 20 | Relation between functional forms of coal nitrogen and NOr emission from pulverized coal combustion显示文摘 | Shinji K Takayuki T Masaru T | 1995 | Fuel1995，74，9： | 1 |
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