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182篇 您的检索式：作者名="Shrestha C"

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	题名	作者	年代	出处	被引量
1	Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity显示文摘AIM To establish a model to enrich and characterize stemlike cells from murine normal liver and hepatocellular carcinoma(HCC) cell lines and to further investigate stem-like cell association with epithelial-to-mesenchymal transition(EMT).METHODS In this study,we utilized a stem cell conditioned serumfree medium to enrich stem-like cells from mouse HCC and normal liver cell lines,Hepa 1-6 and AML12,respectively.We isolated the 3-dimensional spheres and assessed their stemness characteristics by evaluating theRNA levels of stemness genes and a cell surface stem cell marker by quantitative reverse transcriptase-PCR(q RTPCR).Next,we examined the relationship between stem cells and EMT using q RT-PCR.RESULTS Three-dimensional spheres were enriched by culturing murine HCC and normal hepatocyte cell lines in stem cell conditioned serum-free medium supplemented with epidermal growth factor,basic fibroblast growth factor and heparin sulfate.The 3-dimensional spheres had enhanced stemness markers such as Klf4 and Bmi1 and hepatic cancer stem cell(CSC) marker Cd44 compared to parental cells grown as adherent cultures.We report that epithelial markers E-cadherin and ZO-1 were downregulated,while mesenchymal markers Vimentin and Fibronectin were upregulated in 3-dimensional spheres.The 3-dimensional spheres also exhibited changes in expression of Snai,Zeb and Twist family of EMT transcription factors.CONCLUSION Our novel method successfully enriched stem-like cells which possessed an EMT phenotype.The isolation and characterization of murine hepatic CSCs could establish a precise target for the development of more effective therapies for HCC.	Aparna Jayachandran Ritu Shrestha Bijay Dhungel I-Tao Huang Marianna Yumi Kawashima Vasconcelos Brian J Morrison Charmaine A Ramlogan-Steel Jason C Steel	2017	World Journal of Stem Cells2017，9，9：	4
2	Geodetic im- aging with airborne LiDAR: The Earth's surface revealed 显示文摘	Glennie C L Carter W E Shrestha R L	2013	Reports on Progress in Physics2013，76，8：	1
3	Enhanced healing of diabetic wounds by subcutaneous administration of human umbilical cord derived stem cells and their conditioned media显示文摘	Shrestha C Zhao L Chen K	2013	Int J Endocrinol2013，2013，59：	1
4	Self-organized Nano-tubes of TiO2-MoO3 with Enhanced Electrochromic Properties 显示文摘	Shrestha N K Nab Y C Tsuchiya H	2009	Chem Commun2009，，15：	1
5	Dual functionality of BODIPY chromophore in porphyrin-sensitized nanocrystalline solar cells显示文摘	Shrestha M Si L Chang C	2012	J Phys Chem C2012，116，10：	1
6	Towards an understanding of the role of Clostridium perfringens toxins in human and animal disease显示文摘	UZAL F A FREEDMAN J C SHRESTHA A	2014	Future Microbiology2014，9，3：	1
7	Study on thepreparation technology of superfine ground powder of Agrocybechaxingu Huang显示文摘	ZHANG M ZHANG C SHRESTHA S	2005	Journal of Food Engineering2005，67，3：	1
8	On a mean- generalized semivariance approach to determining the hedge ratio显示文摘	Chen S S Lee C F Shrestha K	2001	Journal of Futures Markets2001，21，6：	1
9	Synthesis of CuO nanorods, reduction of CuO into Cu nanorods, and diffuse reflectance measurements of CuO and Cu nanoma- terials in the near infrared region 显示文摘	Shrestha K M Sorensen C M Klabunde K J	2010	J Phys Chem C2010，114，14：	1
10	Black carbon's properties and role in the environment: A comprehensive review 显示文摘	SHRESTHA G TRAINA S J SWANSTON C W	2010	Sustainability2010，2，1：	1
11	Helicobacter pylori infection among patients with upper gastrointestinal symptoms:prevalence and relation to endoscopy diagnosis and histopathology显示文摘	SHRESTHA R KOIRALA K RAJ K C	2014	J Family Med Prim Care2014，3，2：	1
12	Cerebrospinal fluid adenosine deaminase levels as a diagnostic marker in tuberculous meningitis in adult Nepalese patients 显示文摘	CHANDER A SHRESTHA C D	2013	Asian Pac J Trop Dis2013，3，：	1
13	Supply and demand-side effects of power sector planning with demand-side management options and SO2 emission constraints 显示文摘	SHRESTHA R M MARPAUNG C O P	2005	Energy Policy2005，33，6：	1
14	Black carbon’s properties and role in the environment: A comprehensive review显示文摘	Shrestha G Traina S Swanston C	2010	Sustainability2010，2，1：	1
15	Possible idiopathic thrombocytopenic purpura associated with natural alpha interferon therapy for chronic hepatitis C infection显示文摘	Shrestha R Mckinley C Bilir BM	1995	Am J Gastroenterol1995，90，12：	1
16	Self-organized TiO2 nanotubes: Visiblelight activation by Ni oxide nanoparticle decoration显示文摘	SHRESTHA N K YANG M NAIl Y C	2010	Electrochem Commun2010，12，：	1
17	Black carbon's proper- ties and role in the environment: a comprehensivereview 显示文摘	Shrestha G Traina S J Swanston C W	2010	Sustainability2010，2，1：	1
18	Black carbon' s properties and role in the environment: A comprehensive review 显示文摘	Shrestha G Traina S J Swanston C W	2010	Sustainability2010，2，1：	1
19	Study on the preparation technology of superfine ground powder of Agrocybe chaxingu Huang 显示文摘	Zhang M Zhang C J Shrestha S	2005	Journal of Food Engineering2005，67，3：	1
20	Determination of Phosphorus Saturation Level in Relation to Clay Content in Formulated Pond Muds显示文摘	Madhav K Shrestha C Lin K	1996	A quacultural Engineering1996，15，6：	1

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