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| 1 | Effects of narrow plant spacing on root distribution and physiological nitrogen use efficiency in summer maize显示文摘The objective of this study was to understand the effects of plant spacing on grain yield and root competition in summer maize(Zea mays L.). Maize cultivar Denghai 661 was planted in rectangular tanks(0.54 m × 0.27 m × 1.00 m) under 27 cm(normal) and 6 cm(narrow) plant spacing and treated with zero and 7.5 g nitrogen(N) per plant. Compared to normal plant spacing, narrow plant spacing generated less root biomass in the 0–20 cm zone under both N rates, slight reductions of dry root weight in the 20–40 cm and 40–70 cm zones at the mid-grain filling stage, and slight variation of dry root weights in the 70–100 cm zone during the whole growth period. Narrow plant spacing decreased root reductive activity in all root zones, especially at the grain-filling stage. Grain yield and above-ground biomass were 5.0% and 8.4% lower in the narrow plant spacing than with normal plant spacing, although narrow plant spacing significantly increased N harvest index and N use efficiency in both grain yield and biomass, and higher N translocation rates from vegetative organs. These results indicate that the reductive activity of maize roots in all soil layers and dry weights of shallow roots were significantly decreased under narrow plant spacing conditions, resulting in lower root biomass and yield reduction at maturity. Therefore, a moderately dense sowing is a basis for high yield in summer maize. | Wenshun Jiang Kongjun Wang Qiuping Wu Shuting Dong Peng Liu Jiwang Zhang | 2013 | The Crop Journal2013，1，1： | 13 |
| 2 | Soil physical properties and maize root growth under different tillage systems in the North China Plain显示文摘The standard cultivation system in the North China Plain is double cropping of winter wheat and summer maize. The main effects of this cultivation system on root development and yield are decreases in soil nutrient content and depth of the plow layer under either long-term no-tillage or rotary tillage before winter wheat sowing and no tillage before summer maize sowing. In this study, we investigated the combined effects of tillage practices before winter wheat and summer maize sowing on soil properties and root growth and distribution in summer maize. Zhengdan 958(ZD958) was used as experimental material, with three tillage treatments: rotary tillage before winter wheat sowing and no tillage before summer maize sowing(RTW + NTM), moldboard plowing before winter wheat sowing and no tillage before summer maize sowing(MPW + NTM), and moldboard plowing before winter wheat sowing and rotary tillage before summer maize sowing(MPW + RTM).Tillage practice showed a significant(P < 0.05) effect on grain yield of summer maize. Grain yields under MPW + RTM and MPW + NTM were 30.6% and 24.0% higher, respectively, than that under RTW + NTM. Soil bulk density and soil penetration resistance decreased among tillage systems in the order RTW + NTM > MPW + NTM > MPW + RTM. Soil bulk densities were 3.3% and 515% lower in MPW + NTM and MPW + RTM, respectively, than that in RTW + NTM, and soil penetration resistances were respectively 17.8% and 20.4% lower,across growth stages and soil depths. Root dry matter and root length density were highest under MPW + RTM, with the resulting increased root activity leading to a yield increase of summer maize. Thus the marked effects of moldboard plowing before winter-wheat sowing on root length density, soil penetration resistance, and soil bulk density may contribute to higher yield. | Baizhao Ren Xia Li Shuting Dong Peng Liu Bin Zhao Jiwang Zhang | 2018 | The Crop Journal2018，6，6： | 10 |
| 3 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 4 | Two selenium tolerant Lysinibacillus sp. strains are capable of reducing selenite to elemental Se efficiently under aerobic conditions显示文摘Microbes play important roles in the transport and transformation of selenium(Se) in the environment, thereby influencing plant resistance to Se and Se accumulation in plant. The objectives are to characterize the bacteria with high Se tolerance and reduction capacity and explore the significance of microbial origins on their Se tolerance, reduction rate and efficiency. Two bacterial strains were isolated from a naturally occurred Se-rich soil at tea orchard in southern Anhui Province, China. The reduction kinetics of selenite was investigated and the reducing product was characterized using scanning electron microscopy and transmission electron microscopy-energy dispersive spectroscopy. The bacteria were identified as Lysinibacillus xylanilyticus and Lysinibacillus macrolides,respectively, using morphological, physiological and molecular methods. The results showed that the minimal inhibitory concentrations(MICs) of selenite for L. xylanilyticus and L. macrolides were 120 and 220 mmol/L, respectively, while MICs of selenate for L.xylanilyticus and L. macrolides were 800 and 700 mmol/L, respectively. Both strains aerobically reduced selenite with an initial concentration of 1.0 mmol/L to elemental Se nanoparticles(SeNPs) completely within 36 hr. Biogenic SeNPs were observed both inside and outside the cells suggesting either an intra-or extracellular reduction process. Our study implied that the microbes from Se-rich environments were more tolerant to Se and generally quicker and more efficient than those from Se-free habitats in the reduction of Se oxyanions. The bacterial strains with high Se reduction capacity and the biological synthesized Se NPs would have potential applications in agriculture, food, environment and medicine. | Ju Zhang Yue Wang Zongyuan Shao Jing Li Shuting Zan Shoubiao Zhou Ruyi Yang | 2019 | Journal of Environmental Sciences2019，31，3： | 7 |
| 5 | Highly active zigzag-like Pt-Zn alloy nanowires with high-index facets for alcohol electrooxidation显示文摘The development of highly efficient Pt-based alloy nanocatalysts is important but remains challenging for fuel cells commercialization.Here,a new class of zigzag-like platinum-zinc (Pt-Zn) alloy nanowires (NWs) with rough surface and controllable composition is reported.The merits of anisotropic one-dimensional nanostructure,stable high-index facets and coordinatively unsaturated Pt sites endow the composition-optimal Pt94Zn6 NWs with a mass activity of 7.2 and 6.2 times higher than that of commercial Pt black catalysts toward methanol/ethanol oxidation,respectively.Alloying-induced d-band electron modulation and lattice strain effects weaken the adsorption strength of poisoning species,which originally enhances the catalytic activity of Pt-Zn NWs.This study provides a new perspective of Pt-Zn electrocatalysts with intrinsic mechanism for enhanced catalytic performance. | Yanchao Xu Xiaoqiang Cui Shuting Wei Qinghua Zhang Lin Gu Fanqi Meng Jinchang Fan Weitao Zheng | 2019 | Nano Research2019，12，5： | 7 |
| 6 | Study on the promotion of lymphocytes in patients with COVID-19 by broad-spectrum chemokine receptor inhibitor vMIP-Ⅱ and its Mechanism of signal transmission in vitro显示文摘Dear Editor,COVID-19 cases showed a significant decrease in the number of peripheral CD4^(+) and CD8^(+)T cells accompanied by overactivation of cells.1 HHV8-derived viral macrophage inflammatory protein vMIP-Ⅱ is the only broad-spectrum chemokine receptor inhibitor that binds to three subtribes of chemokine receptors,which can significantly increase the specific cell immune response in infected organisms and have an effect of reducing plasma viral load on the viremia period.24 Here we report the effect of vMIP-Ⅱ in COVID-19. | Shiyu Li Shuting Liu Zhenyou Jiang Lixia Feng Yong Gao Youyu Chen Anding Xu Wenhua Huang Nuofu Zhang Hanxiao Sun | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 5 |
| 7 | Microcavity top-emission perovskite light-emitting diodes显示文摘Light-emitting diodes(LEDs)based on perovskites show great potential in lighting and display applications.However,although perovskite films with high photoluminescence quantum efficiencies are commonly achieved,the efficiencies of perovskite LEDs are largely limited by the low light out-coupling efficiency.Here,we show that high-efficiency perovskite LEDs with a high external quantum efficiency of 20.2% and an ultrahigh radiant exitance up to 114.9mWcm^(−2) can be achieved by employing the microcavity effect to enhance light extraction.The enhanced microcavity effect and light outcoupling efficiency are confirmed by the study of angle-dependent emission profiles.Our results show that both the optical and electrical properties of the device need to be optimized to achieve high-performance perovskite LEDs. | Yanfeng Miao Lu Cheng Wei Zou Lianghui Gu Ju Zhang Qiang Guo Qiming Peng Mengmeng Xu Yarong He Shuting Zhang Yu Cao Renzhi Li Nana Wang Wei Huang Jianpu Wang | 2020 | Light(Science & Applications)2020，9，1： | 4 |
| 8 | The formation of perovskite multiple quantum well structures for high performance light-emitting diodes显示文摘Recent works showed that high efficient perovskite light-emitting diodes can be achieved from solution-processed,self-organized multiple quantum wells(MQWs)with an energy cascade.We investigate how the mixing of QWs with different band gaps can affect the perovskite LED device performance.We find that the annealing process can significantly affect the constitution of the MQWs films,where the dominant phase can evolve from large band gap QWs to small band gap QWs.The optimal constitution for LED application lies in a transition point of small-n QWs dominant phase to large-n QWs dominant phase,when the MQW film presents highest photoluminescence while still remains uniform film morphology. | Yan Sun Li Zhang Nana Wang Shuting Zhang Yu Cao Yanfeng Miao Mengmeng Xu Hao Zhang Hai Li Chang Yi Jianpu Wang Wei Huang | 2018 | npj Flexible Electronics2018，2，1： | 4 |
| 9 | Prediction of nonlinear pilot-induced oscillation using an intelligent human pilot model显示文摘This paper proposes a method to predict nonlinear Pilot-Induced Oscillation(PIO)using an intelligent human pilot model.This method is based on a scalogram-based PIO metric,which uses wavelet transforms to analyze the nonlinear characteristics of a time-varying system.The intelligent human pilot model includes three modules:perception module,decision and adaptive module,and execution module.Intelligent and adaptive features,including a neural network receptor,fuzzy decision and adaptation,are also introduced into the human pilot model to describe the behavior of the human pilot accommodating the nonlinear events.Furthermore,an algorithm is proposed to describe the procedure of the PIO prediction method with nonlinear evaluation cases.The prediction results obtained by numerical simulation are compared with the assessments of flight test data to validate the utility of the method.The flight test data were generated in the evaluation of the Smart-Cue/Smart-Gain,which is capable of reducing the PIO tendencies considerably.The results show that the method can be applied to predict the nonlinear PIO events by human pilot model simulation. | Shuting XU Wenqian TAN Xiangju QU Cheng ZHANG | 2019 | Chinese Journal of Aeronautics2019，32，12： | 3 |
| 10 | Physicochemical properties and structure of fluid at nano-/micro-interface:Progress in simulation and experimental study显示文摘In modern chemical engineering processes, the involvement of solid/fluid interface is the most important component of process intensification techniques, such as confined membrane separation and catalysis. In the review, we summarized the research progress of the latest theoretical and experimental works to elucidate the contribution of interface to the fluid properties and structures at nano-and micro-scale. We mainly focused on water, alcohol aqueous solution, and ionic liquids, because they are classical systems in interfacial science and/or widely involved in the industrialization process. Surface-induced fluids were observed in all reviewed systems and played a critical role in physicochemical properties and structures of outside fluid. It can even be regarded as a new interface, when the adsorption layer has a strong interaction with the solid surface. Finally, we proposed a perspective on scientific challenges in the modern chemical engineering processes and outlined future prospects. | Qingwei Gao Yumeng Zhang Shuting Xu Aatto Laaksonen Yudan Zhu Xiaoyan Ji Xiaohua Lu | 2020 | Green Energy & Environment2020，5，3： | 3 |
| 11 | Design and experimental performance verification of a thermal property test-bed for lunar drilling exploration显示文摘Chinese Chang'e lunar exploration project aims to collect and return subsurface lunar soil samples at a minimum penetration depth of 2 m in 2017. However, in contrast to those on the Earth, automated drilling and sampling missions on the Moon raise the risk of burning bits.Test-beds are required for testing the thermal properties of drill tools in a lunar environment. In this paper, a novel temperature measuring method based on thermocouples and a slip ring was proposed. Furthermore, a data acquisition system for a drilling process was designed. A vacuous,cryogenic, and anhydrous soil environment simulating the lunar surface was established. A drilling test-bed that can reach a depth of 2.2 m was developed. A control strategy based on online monitoring signals was proposed to improve the drilling performance. Vacuum and non-vacuum experiments were performed to test the temperature rising effect on drill tools. When compared with the non-vacuum experiment, the vacuum temperature rise resulted in a 12 °C increase. These experimental results provide significant support for Chinese lunar exploration missions. | Zhang Tao Zhao Zeng Liu Shuting Li Jinglin Ding Xilun Yin Shen Wang Guoxin Lai Xiaoming | 2016 | Chinese Journal of Aeronautics2016，29，5： | 3 |
| 12 | Small interfering RNA delivery to the neurons near the amyloid plaques for improved treatment of Alzheimer’s disease显示文摘Gene therapy represents a promising treatment for the Alzheimer’s disease(AD). However,gene delivery specific to brain lesions through systemic administration remains big challenge. In our previous work, we have developed an siRNA nanocomplex able to be specifically delivered to the amyloid plaques through surface modification with both CGN peptide for the blood–brain barrier(BBB)penetration and QSH peptide for β-amyloid binding. But, whether the as-designed nanocomplex could indeed improve the gene accumulation in the impaired neuron cells and ameliorate AD-associated symptoms remains further study. Herein, we prepared the nanocomplexes with an siRNA against β-site amyloid precursor protein-cleaving enzyme 1(BACE1), the rate-limiting enzyme of Aβ production, as the therapeutic siRNA of AD. The nanocomplexes exhibited high distribution in the Aβ deposits-enriched hippocampus, especially in the neurons near the amyloid plaques after intravenous administration. In APP/PS1 transgenic mice, the nanocomplexes down-regulated BACE1 in both mRNA and protein levels,as well as Aβ and amyloid plaques to the level of wild-type mice. Moreover, the nanocomplexes significantly increased the level of synaptophysin and rescued memory loss of the AD transgenic mice without hematological or histological toxicity. Taken together, this work presented direct evidences that the design of precise gene delivery to the AD lesions markedly improves the therapeutic outcome. | Qian Guo Xiaoyao Zheng Peng Yang Xiaoying Pang Kang Qian Pengzhen Wang Shuting Xu Dongyu Sheng Liuchang Wang Jinxu Cao Wei Lu Qizhi Zhang Xinguo Jiang | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 2 |
| 13 | A chromosome-scale genome assembly of Artemisia argyi reveals unbiased subgenome evolution and key contributions of gene duplication to volatile terpenoid diversity显示文摘Artemisia argyi Le´vl.et Vant.,a perennial Artemisia herb with an intense fragrance,is widely used in traditional medicine in China and many other Asian countries.Here,we present a chromosome-scale genome assembly of A.argyi comprising 3.89 Gb assembled into 17 pseudochromosomes.Phylogenetic and comparative genomic analyses revealed that A.argyi underwent a recent lineage-specificwhole-genomeduplication(WGD)event after divergence fromArtemisia annua,resulting in two subgenomes.Wedeciphered the diploid ancestral genome of A.argyi,and unbiased subgenome evolution was observed.The recent WGD led to a large number of duplicated genes in the A.argyi genome.Expansion of the terpene synthase(TPS)gene family through various types of gene duplication may have greatly contributed to the diversity of volatile terpenoids in A.argyi.In particular,we identified a typical germacrene D synthase gene cluster within the expanded TPS gene family.The entire biosynthetic pathways of germacrenes,(+)-borneol,and(+)-camphor were elucidated in A.argyi.In addition,partial deletion of the amorpha-4,11-diene synthase(ADS)gene and loss of function of ADS homologs may have resulted in the lack of artemisinin production in A.argyi.Our study provides newinsights into the genome evolution of Artemisia and lays a foundation for further improvement of the quality of this important medicinal plant. | Hongyu Chen Miaoxian Guo Shuting Dong Xinling Wu Guobin Zhang Liu He Yuannian Jiao Shilin Chen Li Li Hongmei Luo | 2023 | Plant Communications2023，4，3： | 2 |
| 14 | Recent Progresses in Lanthanide Metal-Organic Frameworks(Ln-MOFs) as Chemical Sensors for Ions, Antibiotics and Amino Acids显示文摘Various ions and antibiotics,widely used in industry and clinical medicine,respectively,are massively discharged to atmosphere and water,resulting in severe pollutions on environment and potential threats to human health.Besides,amino acids,the primary substances for the establishment of proteins,cells and tissues,are crucial to human health.Therefore,seeking effective and practicable materials to detect aforesaid analytes is vitally meaningful.Metal-organic frameworks centered with lanthanide ions(Ln-MOFs),also known as lanthanide coordination polymers,are considered as a charming category of multi-functional hybrid crystalline materials with fascinating structures and incomparable luminescent characteristics.Benefited from their unique merits,Ln-MOFs have been largely developed as excellent luminescent sensors for fast and efficient sensing various analytes.In this review,we aim to introduce some of the recent researches between 2018 to 2022 on Ln-MOFs applied as chemical sensors for ions,antibiotics and amino acids based on luminescent quenching and enhancing effects,and provide an update and summary for the latest progresses in this field. | Yan Yang Shuting Xu Yanli Gai Bo Zhang Lian Chen | 2022 | Chinese Journal of Structural Chemistry2022，41，11： | 2 |
| 15 | Effects of sludge characteristics on electrical resistance and energy consumption during electro-dewatering process显示文摘The increase of electrical resistance(ER)and energy consumption(EC)during the later stage of dewatering is a major problem hindering the development of electro-dewatering(EDW)technology.As the variations of sludge characteristics are significant during the EDW process,the relationships between sludge characteristics and ER and EC during EDW remain unclear.In this study,the effects of moisture content(MC),thickness,pH,conductivity,zeta potential,temperature,and gas volume on the ER and EC during the EDW process were statistically investigated using correlation and multiple linear regression analyses.Herein,the results showed that the ER of the sludge near the anode was primarily affected by pH,whereas the sludge near the cathode was primarily affected by the MC and conductivity.Further,sludge temperature and conductivity were the most reliable indicators to predict the EC near the anode and cathode,respectively.The results of this study provide theoretical guidance useful for solving the increase of ER and EC during the later stage of the EDW process. | Li Sha Xiaoyan Yu Zhangxiang Wu Xingxin Liu Shuting Zhang | 2021 | Journal of Environmental Sciences2021，33，3： | 2 |
| 16 | Genomic evidence of lung carcinogenesis associated with coal smoke in Xuanwei area, China显示文摘Xuanwei area, in southwestern China,harbors the highest female lung cancer rate in the country(Supplementary Table 1) [1]. Epidemiological studies have shown that lung cancer incidence in Xuanwei area is associated with the use of different types of local coal for household cooking and heating [2]. The genomic landscape of Xuanwei female adenocarcinoma(XWFA), the most distinctive feature of lung cancer in Xuanwei, has yet to be elucidated systematically. Here,we provide the fundamental resource of genomic datasets for further exploration of XWFA molecular mechanisms and development of targetable therapy. | Honglei Zhang Chao Liu Li Li Xu Feng Qing Wang Jihua Li Shaobin Xu Shuting Wang Qianlu Yang Zhenghai Shen Jinhua Su Xiaosan Su Ruifen Sun Xuhong Zhou Junliang Wang Yongchun Zhou Baowei Jiao Wanbao Ding Xianbao Cao Yue Wang Yunchao Huang Lianhua Ye | 2021 | National Science Review2021，8，12： | 2 |
| 17 | Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity显示文摘Due to the insufficient long-term protection and significant efficacy reduction to new variants of current COVID-19 vaccines,the epidemic prevention and control are still challenging.Here,we employ a capsid and antigen structure engineering(CASE)strategy to manufacture an adenoassociated viral serotype 6-based vaccine(S663V-RBD),which expresses trimeric receptor binding domain(RBD)of spike protein fused with a biological adjuvant RS09.Impressively,the engineered S663V-RBD could rapidly induce a satisfactory RBD-specific IgG titer within 2 weeks and maintain the titer for more than 4 months.Compared to the licensed BBIBP-CorV(Sinopharm,China),a single-dose S663V-RBD induced more endurable and robust immune responses in mice and elicited superior neutralizing antibodies against three typical SARS-CoV-2 pseudoviruses including wild type,C.37(Lambda)and B.1.617.2(Delta).More interestingly,the intramuscular injection of S663V-RBD could overcome pre-existing immunity against the capsid.Given its effectiveness,the CASE-based S663VRBD may provide a new solution for the current and next pandemic. | Fuhua Wu Shuang Luo Yongshun Zhang Yangsen Ou Hairui Wang Zhaofei Guo Chunting He Shuting Bai Penghui He Min Jiang Xiaoyan Chen Guangsheng Du Xun Sun | 2023 | Acta Pharmaceutica Sinica B2023，13，5： | 2 |
| 18 | Electrodynamic Tethered Deorbit System Dynamics and Performance Analysis显示文摘Electrodynamic tethered deorbit technology is a novel way to remove abandoned spacecrafts like upper stages or unusable satellites. This paper investigates and analyses the deorbit performance and mission applicability of the electrodynamic tethered system. To do so, the electrodynamic tethered deorbit dynamics with multi-perturbation is firstly formulated, where the Earth magnetic field, the atmospheric drag, and the Earth oblateness effect are considered. Then, the key system parameters, including payload mass, tether length and tether type, are analyzed by numerical simulations to investigate their influences on the deorbit performance and to give the setting principles for choosing system parameters. Based on this and given an appropriate group of system parameters, numerical simulations are undertaken to study the impact of the mission parameters, including orbit height and orbit inclination, and thus to investigate the mission applicability of the electrodynamic tethered deorbit technology. | ZHANG Feng SHEN Lin WU Shengbao WANG Xiaoding WANG Shuting YU Bensong | 2016 | Aerospace China2016，17，3： | 2 |
| 19 | How delaying post-silking senescence in lower leaves of maize plants increases carbon and nitrogen accumulation and grain yield显示文摘Planting maize at high densities leads to early leaf senescence,and the resulting reduction in the number of lower leaves affects the plant’s root function and lowers its grain yield.However,the nature of the process by which lower leaf senescence affects biomass accumulation and grain yield formation in maize is not clear.This study aimed to shed light on how these factors are related by investigating the effects of the plant growth regulator 6-benzyladenine(6-BA)on the senescence of lower leaves of maize plants.In two maize cultivars planted at densities of 67,500(low density,LD)and 90,000(high density,HD)plants ha^(-1),plants treated with 6-BA maintained a high green leaf area index(LAI)longer than control(CK)plants,enabling them to maintain a higher photosynthetic rate for a longer period of time and produce more biomass before reaching physiological maturity.Spraying the lower leaves of maize plants with a 6-BA solution increased the distribution of;C-photosynthates to their roots,lower leaves and bracts,a result that can be ascribed to a decreased retention of;C-photosynthates in the stem and grain.In both seasons of the experiment,maize plants treated with 6-BA accumulated more N in grain and maintained a higher N content in roots and leaves,especially in lower leaves,than CK.Increased C assimilation in the lower leaves may explain why N uptake in plants subjected to the 6-BA treatment exceeded that in CK plants and why both photosynthesis rate and dry matter accumulation were maintained throughout grain filling.Our results suggest that a suitable distribution of C and N in leaves post-silking may maintain plant root function,increase N use efficiency,maximize the duration of high LAI,and increase grain yield. | Rongfa Li Dandan Hu Hao Ren Qinglong Yang Shuting Dong Jiwang Zhang Bin Zhao Peng Liu | 2022 | The Crop Journal2022，10，3： | 2 |
| 20 | RNA Methylome Reveals the m^(6)A-mediated Regulation of Flavor Metabolites in Tea Leaves under Solar-withering显示文摘The epitranscriptomic mark N6-methyladenosine(m^(6)A),which is the predominant internal modification in RNA,is important for plant responses to diverse stresses.Multiple environmental stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea flavor.However,the effects of the m^(6)A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively uncharacterized.We performed an integrated RNA methylome and transcriptome analysis to explore the m^(6)Amediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea(Camellia sinensis)leaves under solar-withering conditions.Dynamic changes in global m^(6)A level in tea leaves were mainly controlled by two m^(6)A erasers(CsALKBH4A and CsALKBH4B)during solar-withering treatments.Differentially methylated peak-associated genes following solarwithering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome pathways.Further analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid,catechin,and theaflavin contents by triggering alternative splicing-mediated regulation.Our findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m^(6)A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions. | Chen Zhu Shuting Zhang Chengzhe Zhou Caiyun Tian Biying Shi Kai Xu Linjie Huang Yun Sun Yuling Lin Zhongxiong Lai Yuqiong Guo | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 2 |
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