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| 1 | Inhibitory effect of human telomerase antisense oligodeoxyribonucleotides on the growth of gastric cancer cell lines in variant tumor pathological subtype显示文摘AIM: To investigate the inhibitory effect of specialized human telomerase antisense oligodeoxyribonucleotides on the growth of well (MKN-28), moderately (SGC-7901)and poorly (MKN-45) differentiated gastric cancer cell lines under specific conditions and its inhibition mechanism,and to observe the correlation between the growth inhibition ratio and the tumor pathologic subtype of gastric cancer cells.METHODS: Telomerase activity in three gastric cancer cell lines of variant tumor pathologic subtype was determined by modified TRAP assay before and after the specialized human telomerase antisense oligodeoxyribonucleotides were dealt with under specific conditions. Effect of antisense oligomer under specific conditions of the growth and viability of gastric cancer cell lines was explored by using trypan blue dye exclusion assay, and cell apoptosis was detected by cell morphology observation, flow cytometry and TUNEL assay.RESULTS: Telomerase activity was detected in well,moderately and poorly differentiated gastric cancer cell lines (the quantification expression of telomerase activity was 43.7TPG, 56.5TPG, 76.7TPG, respectively).Telomerase activity was controlled to 30.2TPG, 36.3TPG and 35.2TPG for MKN-28, SGC-7901 and MKN-45 cell lines respectively after treatment with human telomerase antisense oligomers at the concentration of 5 μmol/L, and was entirely inhibited at 10 μmol/L, against the template region of telomerase RNA component, whereas no inhibition effect was detected in missense oligomers (P<0.05). After treatment with antisense oligomers at different concentrations under specific conditions for 96 h, significant growth inhibition effects were found in MKN-45 and SGC-7901gastric cancer cell lines (the inhibition ratio was 40.89%and 71.28%), but not in MKN-28 cell lines (15.86%). The ratio of inactive SGC-7901 cells increased according to the prolongation of treatment from 48 to 96 h. Missense oligomers could not lead to the same effect (P<0.05).Apoptosis of SGC-7901 and MKN-45 cells was detected not only by morphology and TUNEL assay but also by flow cytometry. The apoptotic rate reached 33.56% for SGC-7901 cells and 44.75% for MKN-45 cells.CONCLUSION: The viability and proliferation of gastric cancer cells can be inhibited by antisense telomerase oligomers. The growth inhibition of gastric cancer cells is correlated with concentration, time and sequence specialty of antisense oligomers. The inhibition mechanism of antisense human telomerase oligomers depends not only on the sequence specialty but also on the biological characteristics of gastric cancer cell lines. | JingYe Yun-LinWu ShuZhang ZiChen Li-XiaGuo Ruo-YuZhou HongXie | 2005 | World Journal of Gastroenterology2005，11，15： | 9 |
| 2 | Synergistic effects of elevated homocysteine level and abnormal blood lipids on the onset of stroke显示文摘Hyperhomocysteinemia and abnormal blood lipids are independent risk factors for stroke.However,whether both factors exert a synergistic effect in the onset of stroke remains unclear.The present study is a retrospective analysis of 2 089 cases of stroke and 2 089 control cases of simple intervertebral disk protrusion using a paired multivariate logistic regression method.Adjusting for known confounding variables including the patients’age,gender,smoking status,alcohol consumption status,patient and family medical history,and clinical biochemical indices,elevated homocysteine level was related to the onset of stroke.Patients with elevated homocysteine levels and abnormal blood lipids showed a 40.9%increase in the risk for stroke compared to patients with normal homocysteine levels and blood lipids(odds ratio 1.409;95%confidence interval1.127–1.761).These results indicate that elevated homocysteine and abnormal blood lipids exert synergistic effects in the onset of stroke.Patients with elevated homocysteine levels and abnormal blood lipids are predisposed to stroke. | Lu Hao Liming Chen Xiaoyong Sai Zhefeng Liu Guang Yang Rongzeng Yan Lili Wang Caiyun Fu Xuan Xu Zhenzhen Cheng Qiang Wu Shuzhang Li | 2013 | Neural Regeneration Research2013，8，31： | 9 |
| 3 | Clinical significance of serum IGF-I,IGF-II and IGFBP-3 in liver cirrhosis显示文摘AIM: To investigate the relationship between insulin-like growth factor-Ⅰ, -Ⅱ (IGF-Ⅰ and IGF-Ⅱ), IGF-binding protein 3(IGFBP-3) and Child-Pugh score in patients with liver cirrhosis,and to search for potential clinical markers of liver function.METHODS: Forty-four patients with advanced liver cirrhosis of viral origin were divided into 3 groups according to severity of cirrhosis (Child-Pugh score) and 38 healthy subjects served as controls. Serum levels of IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 were measured by immunoradiometric assay.RESULTS: Serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 levels were significantly lower in patients with cirrhosis than in controls,and serum concentrations of IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 were associated with the severity of liver dysfunction, and dropped sharply during the progression of liver failure. Among these 3 parameters, serum IGF-Ⅱ was the most sensitive and effective indicator for liver dysfunction. Concentrations of IGF-Ⅰ <30 ng/mL, IGF-Ⅱ <200 ng/mL and IGFBP-3 <6 ng/mL implied a negative prognosis for patients with liver cirrhosis.CONCLUSION: Serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 may provide a new dimension in the assessment of liver dysfunction.Combined detection of serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 with Child-Pugh score is more effective in predicting prognosis than Child-Pugh score alone. | Yun-LinWu JingYe ShuZhang JieZhong Rong-PingXi | 2004 | World Journal of Gastroenterology2004，10，18： | 7 |
| 4 | Promoted conversion of polysulfides by MoO_2 inlaid ordered mesoporous carbons towards high performance lithium-sulfur batteries显示文摘As polar materials, transition-metal oxides have shown great potentials to improve the adsorption of lithium polysulfides in lithium-sulfur batteries. Herein, a MoO_2-ordered mesoporous carbon (M-OMC)hybrid was designed as the sulfur host, in which MoO_2 is inlaid on the surface of ordered mesoporous carbons that can store active materials and provide fast electron transfer channel due to its ordered pore structure. The MoO_2 can effectively prevent the migration of polysulfides through the chemical adsorption and promote the conversion of polysulfides towards Li-sulfur battery. | Yawei Chen Shuzhang Niu Wei Lv Chen Zhang Quanhong Yang | 2019 | Chinese Chemical Letters2019，30，2： | 4 |
| 5 | Drug-drug cocrystals:Opportunities and challenges显示文摘Recently,drug-drug cocrystal attracts more and more attention.It offers a low risk,low-cost but high reward route to new and better medicines and could improve the physiochemical and biopharmaceutical properties of a medicine by addition of a suitable therapeutically effective component without any chemical modification.Having so many advantages,to date,the reported drug-drug cocrystals are rare.Here we review the drug-drug cocrystals that reported in last decade and shed light on the opportunities and challenges for the development of drug-drug cocrystals. | Xiaojuan Wang Shuzhang Du Rui Zhang Xuedong Jia Ting Yang Xiaojian Zhang | 2021 | Asian Journal of Pharmaceutical Sciences2021，16，3： | 2 |
| 6 | The Dynamic Adjustments of Factor Inputs in China~ Major Wheat Producing Areas显示文摘 | CHEN SHUZHANG OXLEY L XU ZHENG WANG YANQING MA HENGYUN | 2013 | Economic Modeling2013，33，1： | 1 |
| 7 | Towards an attenuated enterohemorrahagic Escherichia coli O157:H7 vaccine characterized by a deleted ler gene and containing apathogenic Shiga toxins 显示文摘 | Jun Liu Yang Sun Shuzhang Feng | 2009 | Vaccine2009，，27： | 1 |
| 8 | Towards a vaccine for attaching/effacing Escherichia coli : a LEE encoded regulator ( ler ) mutant of rabbit enteropathogenic Escherichia coli is attenuated, immunogenic, and protects rabbits from lethal challenge with the wild-type virulent strain显示文摘 | Chengru Zhu Shuzhang Feng Timothy E. Thate James B. Kaper Edgar C. Boedeker | 2005 | Vaccine2005，，18： | 1 |
| 9 | Pharmacokinetic evaluation and antitumor activity of 2-methoxyestradiol nanosuspension显示文摘 | Shuzhang Du Ling Zhu Bin Du Xiufang Shi Zhenzhong Zhang Shuyu Wang Chaofeng Zhang | 2012 | Drug Development and Industrial Pharmacy2012，，4： | 1 |
| 10 | Modern ideas and measures of hospital infectionmonitoring and management 显示文摘 | Li Shuzhang | 2010 | Chinese journal of hospitalinfection2010，14，1： | 1 |
| 11 | Asemiotic multi-agent modelling approach for clinical pathway management显示文摘 | Weizi Li Kecheng Liu Shuzhang Li | 2010 | Journal of Computers2010，5，2： | 1 |
| 12 | The ilioinguinal approach and K-L combined with open reduction and internal fixation for the treatment of 47 cases of complex acetabular fractures of显示文摘 | Xi Xueyi Xu Gang Guo Shuzhang | 2013 | Chinese medical innovation2013，17，1： | 1 |
| 13 | Synthesis and biological evaluation of a novel asymmetrical ^99mTc- nitrido complex of metronidazole derivative 显示文摘 | Kong Dejing Lu Jie Ye Shuzhang | 2007 | J Label Compd Radiopharm2007，，： | 1 |
| 14 | A Semiotic Multi-agent Modeling Approach for Clinical Pathway Management显示文摘 | Weizi Li Kecheng Liu Shuzhang Li | 2010 | Journal of Computers2010，5，2： | 1 |
| 15 | A new pH fluorescent molecular switch by modulation of twisted intramolecular charge transfer with pmtonation 显示文摘 | Ping S Changying Y Shuzhang X | 2013 | J Phys Org Chem2013，26，10： | 1 |
| 16 | In situ fabrication of 2D SnS2 nanosheets as a new electron transport layer for perovskite solar cells显示文摘To promote commercialization of perovskite solar cells (PSCs), low-temperatureprocessed electron transport layer (ETL) with high carrier mobility still needs tobe further developed. Here, we reported two-dimensional (2D) tin disulfide(SnS2) nanosheets as ETL in PSCs for the first time. The morphologies of the 2DSnS2 material can be easy controlled by the in situ synthesized method on theconductive fluorine-doped fin oxide (FTO) substrate. We achieved a championpower conversion efficiency (PCE) of 13.63%, with the short-circuit currentdensity (Jsc) of 23.70 mA/cm2 open-circuit voltage (Voc) of 0.95 V, and fill factor(FF) of 0.61. The high Jsc of PSCs results from effective electron collection ofthe 2D SnS2 nanosheets from perovskite layer and fast electron transport tothe FTO. The low Voc and FF are the results of the lower conduction band of 2DSnS2 (4.23 eV) than that of TiO2 (4.0 eV). These results demonstrate that 2Dmaterial is a promising candidate for ETL in PSCs. | Erling Zhao Liguo Gao Shuzhang Yang Likun Wang Junmei Cao Tingli Ma | 2018 | Nano Research2018，11，11： | 1 |
| 17 | Synthesis and characterization of novel fluorescent BOPIM dyes with large Stokes shift 显示文摘 | Miaofu M Shuzhang X Tao Y | 2011 | J Fluorine Chem2011，132，9： | 1 |
| 18 | Structureeproperty correlation of solid-emissive boronefluorine derivatives 显示文摘 | Qiong C Shuzhang X Ku Z | 2012 | J Org Chem2012，717，： | 1 |
| 19 | 显示文摘 | Jia Baofu Liu Shuzhang Lin Weigan | 1990 | J of Electronics1990，12，5： | 1 |
| 20 | Effects of simulated microgravity on the alkaline phosphatase activity and intracellular calcium concentration of cultured chondrocytes显示文摘The effects of simulated microgravity on matrix mineralization of chondrocytes were examined using cultured chicken embryonic chondrocytes as the model. In four days, there was a time course decrease in alkaline phosphatase activity of chondrocytes, a marker of matrix mineralization.Meanwhile, in two days, there was a significant drop in intracellular calcium concentration in contrast to the control. These results indicate that simulated microgravity can suppress matrix calcification of cultured chondrocytes, and intracellular calcium may be involved in the regulation of matrix calcification as the second messenger. | Xiaobing Li Shuzhang Yang Shengguang Li Peidong Jiang Zhihuan Lin | 1999 | Chinese Science Bulletin1999，44，3： | 0 |
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