1篇
您的检索式：作者名="Stefan Mester"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The Role of Quality Control in Targeted Next-generation Sequencing Library Preparation显示文摘Next-generation sequencing(NGS) is getting routinely used in the diagnosis of hereditary diseases, such as human cardiomyopathies. Hence, it is of utter importance to secure high quality sequencing data, enabling the identi?cation of disease-relevant mutations or the conclusion of negative test results. During the process of sample preparation, each protocol for target enrichment library preparation has its own requirements for quality control(QC); however, there is little evidence on the actual impact of these guidelines on resulting data quality. In this study, we analyzed the impact of QC during the diverse library preparation steps of Agilent Sure Select XT target enrichment and Illumina sequencing. We quanti?ed the parameters for a cohort of around 600 samples, which include starting amount of DNA, amount of sheared DNA, smallest and largest fragment size of the starting DNA; amount of DNA after the pre-PCR, and smallest and largest fragment size of the resulting DNA; as well as the amount of the ?nal library, the | Rouven Nietsch Jan Haas Alan Lai Daniel Oehler Stefan Mester Karen S. Frese Farbod Sedaghat-Hamedani Elham Kayvanpour Andreas Keller Benjamin Meder | 2016 | Genomics, Proteomics & Bioinformatics2016，14，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员