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| 1 | Clp Protease and OR Directly Control the Proteostasis of Phytoene Synthase, the Crucial Enzyme for Carotenoid Biosynthesis in Arabidopsis显示文摘Phytoene synthase (PSY ) 是在类胡萝卜素 biosynthetic 小径的关键 plastidial 酶。然而,它的 translational 以后规定留下逃犯。同样, Clp 朊酶组成质体朊酶网络的中央部分,但是它为降级的底层不是众所周知的。在这研究,我们报导 PSY 是 Clp 朊酶的底层。PSY 被揭开身体上与各种各样的 Clp 朊酶子单元交往(即, ClpS1, ClpC1,和 ClpD ) 。在 clpc1, clpp4,和 clpr1-2 异种的 PSY 和几另外的 carotenogenic 酶蛋白质 overaccumulate 高级。overaccumulated PSY 被发现部分酶地活跃。在 clpc1 的 Clp 活动的缺陷导致 PSY 蛋白质周转的减少的率,进一步在降级的 PSY 支持 Clp 朊酶的角色蛋白质。在另一方面, ORANGE (或) 蛋白质,有 holdase 女伴活动的 PSY 的一个主要 translational 以后管理者,提高 PSY 蛋白质稳定性并且在 clpc1 增加 PSY 的酶地活跃的比例, counterbalancing 在维持 PSY 蛋白质动态平衡的调停 Clp 的解朊作用。一起,这些调查结果提供新奇卓见进质体局部性的蛋白质的质量控制并且建立迄今未辩别出的 translational 以后在在植物的 modulating 类胡萝卜素生合成的 carotenogenic 酶的规章的机制。 | Ralf Welsch Xiangjun Zhou Hui Yuan Daniel Alvarez Tianhu Sun Dennis Schlossarek Yong Yang Guoxin Shen Hong Zhang Manuel Rodriguez-Concepcion Theodore W. Thannhauser Li Li | 2018 | Molecular Plant2018，11，1： | 10 |
| 2 | The Or Gene Enhances Carotenoid Accumulation and Stability During Post-Harvest Storage of Potato Tubers显示文摘在主食庄稼的维生素原 A 类胡萝卜素不在存储期间是很稳定的,他们的损失损害营养的质量。阐明位于类胡萝卜素累积和稳定性下面的基本机制,我们调查了表示了花椰菜橘子的转基因的土豆 tubers (或) 基因。我们发现 Or transgene 不仅支持了叶红素水平的保留,而且连续地在冷存储的 5months 期间刺激了它的累积。相反,类胡萝卜素的增加的层次都没在存储的一样的时期期间在向量唯一的控制或一个黄肉的变化的 tubers 被观察。增加的类胡萝卜素累积被发现与扣押的 lipoproteincarotenoid 的形成被联系结构,以及与提高的许多 phytoene synthase,在类胡萝卜素 biosynthetic 小径的关键酶。而且,存储的维生素原 A 类胡萝卜素被显示在模仿的消化期间稳定并且为由人的肠的吸收性的房间的举起可存取。Proteomic 分析识别了蛋白质的三个主要的功能的组(即热吃惊蛋白质, glutathione-S-transferases,和糖类新陈代谢的蛋白质) 那在或调整类胡萝卜素累积是潜在地重要的。我们的结果证明类胡萝卜素隐遁能力的那条规定是类胡萝卜素稳定性被调整的重要机制。我们的调查结果建议在主食庄稼的合适的水池结构形成的那正式就职可以向庄稼提供在植物生长和收获以后的存储期间支持或稳定维生素原 A 累积的一个唯一的能力。 | Li Li Yong Yang Qiang Xu Katherine Owsiany Ralf Welsch Chureeporn Chitchumroonchokchai Shan Lu Joyce Van Eck Xiu-Xin Deng Mark Failla Theodore W. Thannhauser | 2012 | Molecular Plant2012，5，2： | 8 |
| 3 | Development of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots显示文摘Single-cell-type proteomics provides the capability to revealing the genomic and proteomics information at cell-level resolution.However,the methodology for this type of research has not been well-developed.This paper reports developing a workflow of laser capture microdissection(LCM)followed by gel-liquid chromatography-tandem mass spectrometry(GeLC-MS/MS)-based proteomics analysis for the identification of proteomes contained in individual cell layers of tomato roots.Thin-sections(~10-μm thick,10 sections per root tip)were prepared for root tips of tomato germinating seedlings.Epidermal and cortical cells(5000–7000 cells per tissue type)were isolated under a LCM microscope.Proteins were isolated and then separated by SDS–polyacrylamide gel electrophoresis followed by in-gel-tryptic digestion.The MS and MS/MS spectra generated using nanoLC-MS/MS analysis of the tryptic peptides were searched against ITAG2.4 tomato protein database to identify proteins contained in each single-cell-type sample.Based on the biological functions,proteins with proven functions in root hair development were identified in epidermal cells but not in the cortical cells.Several of these proteins were found in Al-treated roots only.The results demonstrated that the cell-type-specific proteome is relevant for tissue-specific functions in tomato roots.Increasing the coverage of proteomes and reducing the inevitable cross-contamination from adjacent cell layers,in both vertical and cross directions when cells are isolated from slides prepared using intact root tips,are the major challenges using the technology in proteomics analysis of plant roots. | Yingde Zhu Hui Li Sarabjit Bhatti Suping Zhou Yong Yang Tara Fish Theodore W Thannhauser | 2016 | Horticulture Research2016，3，1： | 2 |
| 4 | Analysis for disulfide bonds in peptides and proteins显示文摘 | THANNHAUSER T W KONISHI Y SCHERAGA H A | 1987 | Methods in Enzymology1987，143，： | 1 |
| 5 | Theisolation of L-α-glycerylphosphorylcholine from incuba-ted beef pancreas:its significance for the intermediary me-tabolism of lecithin显示文摘 | SCHMIDT G HERSHMAN B THANNHAUSER S J | 1945 | J Biol Chem1945，161，： | 1 |
| 6 | The isolation of c~-glyceryphosphorylcholine from incubated beef pancreas: its significance for the intermediary metabolism of lecithin 显示文摘 | Schmidt G Hershman B Thannhauser SJ | 1945 | J Biol Chem1945，161，： | 1 |
| 7 | Kinetic studies of the regeneration of recombinant hirudin variant 1 with oxidized and reduced dithiothreitol显示文摘 | THANNHAUSER T W ROTHWARF D M SCHERAGA H A | 1997 | Biochem- istry1997，36，8： | 1 |
| 8 | Coupling genetics and proteomics to identify aphid proteins associated with vector-specific transmission of polerovirus (Luteoviridae)显示文摘 | Yang X Thannhauser T W Burrows M Cox-Foster D Gildow F E Gray S M | | 0，，： | 1 |
| 9 | A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues 显示文摘 | SCHMIDT G THANNHAUSER S J | 1945 | Journal of Biological Chemistry1945，161，： | 1 |
| 10 | Sensitive quantitative analysis of disulfide bonds in polypeptides and proteins 显示文摘 | Thannhauser T W Konishi Y & Scheraga H A | 1984 | Analytical Chemistry1984，138，： | 1 |
| 11 | The isolation of α-glycerylphosphorylcholine from incubated beef pancreas:its significance for the intermediary metabolism of lecithin显示文摘 | SCHMIDT G HERSHMAN B THANNHAUSER S J | 1945 | J Biol Chem1945，161，： | 1 |
| 12 | Physiology of cholesterol metabolism in man显示文摘 | Thannhauser SJ | 1950 | J Mt Sinai Hosp N Y1950，17，2： | 1 |
| 13 | Verification of 50- to 100-mer DNA and RNA sequences with high- resolution mass spectrometry显示文摘 | Little DP Thannhauser TW McLafferty FW | 1995 | Proc Nat1 Acad Sci U S A1995，92，6： | 1 |
| 14 | Sensitive quantitative analysis of disulfide bonds in polypeptides and proteins 显示文摘 | Thannhauser T W Konishi Y Scheraga H A | 1984 | Analytical Biochemistry1984，138，1： | 1 |
| 15 | Phytoene desaturase is present in a large protein complex in the plastid membrane显示文摘 | Lopez A B Yang Y Thannhauser T W | 2008 | Physiologia Plantarum2008，133，： | 1 |
| 16 | A method for the determination of desoxyribonucleic acid,ribonucleic acid,and phosphoproteins in animal tissues显示文摘 | Schmidt G Thannhauser S J | | 0，，01： | 1 |
| 17 | Parental stressand quality of life in children with neuromuscular disease 显示文摘 | Mah JK Thannhauser JE Kolski H | 2008 | Pediatr Neurol2008，39，2： | 1 |
| 18 | The isolation ofα-glycerylphosphorylcholine from incubated beef pancreas:Its significance for the intermediary metabolism of lecithin显示文摘 | Schmidt G Hershman B Thannhauser SJ | 1945 | J Biol Chem1945，161，： | 1 |
| 19 | A method for the deter- mination of desoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues显示文摘 | Schimidt G Thannhauser S | 1945 | Journal of Bio- logical Chemistry1945，161，： | 1 |
| 20 | The isolation of alpha glycerylphosphorylcholine from incubated beef pancreas:Its significance for the intermediary metabolism of lecithin显示文摘 | Schmidt G Hershman B Thannhauser SJ | 1945 | J Biol Chem1945，161，： | 1 |
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