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		题名	作者	年代	出处	被引量
	1	Monoclonal Antibody-Based Enzyme Linked Immunosorbent Assay for the Analysis of Jasmonates in Plants显示文摘Methyl jasmonate (MeJA) and its free-acid form, jasmonic acid (JA) are naturally occurring plant growth regulators widely distributed in higher plants. In order to improve the sensitivity for the analysis of MeJA at low levels in small amounts of plant samples, a monoclonal antibody (MAb) (designated as MAb 3E5D7C4B6) against MeJA was derived from a JA-bovine serum albumin (BSA) conjugate as an immunogen. The antibody belongs to the IgG1 subclass with a κ type light chain and has a dissociation constant of approximately 6.07 × 10?9 M. MAb3E5D7C4B6 is very specific to MeJA. It was used to develop a direct competitive enzyme-linked immunosorbent assay (dcELISA), conventional and simplified indirect competitive ELISAs (icELISA). JA was derivatized into MeJA for the ELISA analysis. The IC50 value and detection range for MeJA were, respectively, 34 and 4-257 ng/mL by the conventional icELISA, 21 and 3-226 ng/mL by the simplified icELISA and 5.0 and 0.7-97.0 ng/mL by the dcELISA. The dcELISA was more sensitive than either the conventional or simplified icELISA. The assays were used to measure the content of jasmonates as MeJA in tobacco leaves under drought stress or inoculated with tobacco mosaic virus and tomato leaves inoculated with tomato mosaic virus or Lirioinyza sativae Blanchard as compared with the corresponding healthy leaves. The increased jasmonates content indicated its role in response to the drought stress and pathogens.	Aixing Deng Weiming Tan Suping He Wei Liu Tiegui Nan Zhaohu Li Baomin Wang Qing X. Li	2008	Journal of Integrative Plant Biology2008，50，8：	9
	2	Development of a sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the analysis of cadmium ions in water, soil and rape samples显示文摘	Wei Gao Tiegui Nan Guiyu Tan Hongwei Zhao Baomin Wang QingX. Li Fanyun Meng	2012	Food and Agricultural Immunology2012，，1：	2
	3	Mechanism and enhancement of the surface stress caused by a small-molecule antigen and antibody binding 显示文摘	Wu Shangquan Nan Tiegui Xue Changguo	2013	Biosensors and Bioelectronics2013，48，：	1
	4	Development of two highly sensitive immunoassays for detection of copper ions and a suite of relevant immunochemicals显示文摘	Hongwei Zhao Tiegui Nan Guiyu Tan Wei Gao Zhen Cao Shuo Sun Zhaohu Li Qing X. Li Baomin Wang	2011	Analytica Chimica Acta2011，，1：	1
	5	Detection of copper ions using microcantilever immunosensors and enzyme-linked immunosorbent assay显示文摘	Hongwei Zhao Changguo Xue Tiegui Nan Guiyu Tan Zhaohu Li Qing X. Li Qingchuan Zhang Baomin Wang	2010	Analytica Chimica Acta2010，，1：	1
	6	De- tection of copper ions using microcantilever immunosew sors and enzyme-linked immunosorbent assay显示文摘	Zhao Hongwei Xue Changguo Nan Tiegui	2010	Ana- lytica Chimica Acta2010，676，12：	1
	7	Devel- opment of protein a functionalized microcantilever im- munosensors for the analyses of small molecules at part per trillion levels 显示文摘	Tan Weiming Huang Yuan Nan Tiegui	2010	Analysis Chemistry2010，82，2：	1

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