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您的检索式：作者名="Tomas Pluskal"
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| 1 | The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis显示文摘Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots,'Huang Qin,' are used for liver and lung complaints and as complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408.14-Mb genome has been assembled into nine pseudochromosomes with a super-N50 of 33.2 Mb. Comparison of this sequence with those of closely related species in the order Lamiales, sesamum indicum and Salvia splendens,revealed that a specialized metabolic pathway for the synthesis of 4'-deoxyflavone bioactives evolved in the genus Scu-tellaria. We found that the gene encoding a specific cinnamate coenzyme A ligase likely obtained its newfunc- tion following recent mutations, and that four genes encoding enzymes in the 4'-deoxyflavone pathway are present as tandem repeats in the genome of S. baicalensis. Further analyses revealed that gene duplications, segmental duplication, gene amplification, and point mutations coupled to gene neo- and subfunctionaliza-tions were involved in the evolution of 4'-deoxyflavone synthesis in the genus Scutellaria. Our study not only provides significant insight into the evolution of specific flavone biosynthetic pathways in the mint family, Lamiaceae, but also will facilitate the development of tools for enhancing bioactive productivity by metabolic engineering in microbes or by molecular breeding in plants. The reference genome of S. baicalensis is also useful for improving the genome assemblies for other members of the mint family and offers an important foundation for decoding the synthetic pathways of bioactive compounds in medicinal plants. | Qing Zhao Jun Yang Meng-Ying Cui Jie Liu Yumin Fang Mengxiao Yan Wenqing Qiu Huiwen Shang Zhicheng Xu Reheman Yidiresi Jing-Ke Weng Tomas Pluskal Marielle Vigouroux Burkhard Steuernagel Yukun Wei Lei Yang Yonghong Hu Xiao-Ya Chen Cathie Martin | 2019 | Molecular Plant2019，12，7： | 23 |
| 2 | Complete Pathway Elucidation and Heterologous Reconstitution of Rhodiola Salidroside Biosynthesis显示文摘Salidroside 是导出酷氨酸的酉分的自然产品在 Rhodiola 类下面在药用的植物发现了的 bioactive。除了他们在传统的药的反疲劳和反血缺氧角色, Rhodiola 全部的摘录和 salidroside 也作为反心血管的疾病和反癌症代理人显示了药用的性质。在 Rhodiola 植物和 salidroside 的全球需求的产生巨浪驱使某种类靠近到扑灭。这里,我们报导为 Rhodiola rosea 利用第一全面 transcriptomics 和 metabolomics 数据集的 Rhodiola salidroside biosynthetic 小径的完整的说明。不同于包含分开的去碳酸基和 deamination 的以前建议的小径从酷氨酸的酶的步到关键中间的 4-hydroxyphenylacetaldehyde (4-HPAA ) , Rhodiola 包含 pyridoxal 磷酸盐依赖者 4-HPAA synthase ? 那直接把酷氨酸变换成 4-HPAA。我们推进 ? 编码随后的 4-HPAA reductase 的识别基因 ? 并且 tyrosol :UDP 葡萄糖 8-O-glucosyltransferase,分别地在 Rhodiola 完成 salidroside 生合成。我们证明 salidroside 的那 heterologous 生产能通过 Rhodiola salidroside biosynthetic 基因的转基因的表示在酵母 Saccharomyces cerevisiae 以及植物 Nicotiana benthamiana 被完成。这研究为在 heterologous 主人设计 salidroside 的持续生产提供新工具。 | Michael P. Torrens-Spence Tomas Pluskal Fu-Shuang Li Valentina Carballo Jing-Ke Weng | 2018 | Molecular Plant2018，11，1： | 14 |
| 3 | PBS3 and EPS1 Complete Salicylic Acid Biosynthesis from Isochorismate in Arabidopsis显示文摘Salicylic acid(SA)is an important phytohormone mediating both local and systemic defense responses in plants.Despite over half a century of research,how plants biosynthesize SA remains unresolved.In Arabidop-sis,a major part of SA is derived from isochorismate,a key intermediate produced by the isochorismate syn-thase,which is reminiscent of SA biosynthesis in bacteria.Whereas bacteria employ an isochorismate pyru-vate lyase(IPL)that catalyzes the turnover of isochorismate to pyruvate and SA,plants do not contain an IPL ortholog and generate SA from isochorismate through an unknown mechanism.Combining genetic and biochemical approaches,we delineated the SA biosynthetic pathway downstream of isochorismate in Ara-bidopsis.We found that PBS3,a GH3 acyl adenylase-family enzyme important for SA accumulation,catalyzes ATP-and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate,isochorismoyl-glutamate A.Moreover,we discovered that EPS1,a BAHD acyltransferase-family protein with a previously implicated role in SA accumulation upon pathogen attack,harbors a noncanonical active site and an unprecedented isochorismoyl-glutamate A pyruvoyl-glutamate lyase activity that produces SA from the isochorismoyl-glutamate A substrate.Together,PBS3 and EPS1 form a two-step metabolic pathway to produce SA from isochorismate in Arabidopsis,which is distinct from how SA is biosynthesized in bacteria.This study closes a major knowledge gap in plant SA meta-bolism and would help develop new strategies for engineering disease resistance in crop plants. | Michael P.Torrens-Spence Anastassia Bobokalonova Valentina Carballo Christopher MGlinkerman Toma(s)Pluskal Amber Shen Jing-Ke Weng | 2019 | Molecular Plant2019，12，12： | 10 |
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