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| 1 | A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity显示文摘The complete genome of Marek's disease virus (MDV) strain GX0101, which was integrated with the LTR sequences of REV, was cloned in Escherichia coli as a bacterial artificial chromosome (BAC). BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination. The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B. Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF). This BAC viral clone was named bac-GX0101. When the reconstituted virus was inoculated into 1-day-old birds, visceral tumors could be detected as early as 62 d post infection. There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus. The BAC derived virus maintained its oncogenicity and immunosuppressive effects. In conclusion, the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued. With the powerful BAC manipulation system, the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV. | SUN AiJun LAWRENCE Petherbridge ZHAO YuGuang LI YanPeng NAIR Venugopal K CUI ZhiZhong | 2009 | Chinese Science Bulletin2009，54，15： | 15 |
| 2 | 带有REV-LTR片段的MDV野毒株GX0101 BAC克隆的构建及拯救病毒的致病性分析显示文摘将整合有禽网状内皮组织增生症病毒(reticuloendotheliosis,REV)长末端重复序列(LTR)的马立克氏病病毒(命名为GX0101)全基因组作为细菌人工染色体(bacterial artificial chromosome,BAC)克隆进大肠杆菌.BAC载体通过同源重组插入MDV基因组的US2区,含BAC载体的MDV DNA电转化大肠杆菌菌株DH10B,鉴定含MDV全基因组的BAC克隆(MDV BAC),提取BAC DNA,转染原代鸡胚成纤维细胞(CEF),成功拯救出了重组病毒,命名为bac-GX0101.将此病毒腹腔接种1日龄鸡,在攻毒后62d开始检测到内脏肿瘤,动物实验结果表明,bac-GX0101毒株与亲本GX0101毒株对机体的致病性等方面的影响没有差异,bac-GX0101毒株仍然保留着很好的免疫抑制功能及致肿瘤能力.该感染性克隆bac-GX0101为研究该重组野毒株中REV-LTR插入片段及其他基因的生物学功能提供了有用的技术平台. | 孙爱军 PETHERBRIDGE Lawrence ZHAO YuGuang 李延鹏 NAIR Venugopal K 崔治中 | 2009 | 科学通报2009，54，11： | 7 |
| 3 | Microstructure and tensile properties of Inconel 718 pulsed Nd-YAG laser welds 显示文摘 | JANAKI RAM G D VENUGOPAL A PRASAD K | 2005 | Journal of Material Processing Technology2005，167，： | 1 |
| 4 | A self-adaptive migration model genetic algorithm for data mining applications显示文摘 | Srinivasa K Venugopal K Patnaik L | 2007 | Information Sciences2007，177，20： | 1 |
| 5 | Synthesis of copper-alumina nanocomposite by reactive milling显示文摘 | Venugopal T Prasad Rao K Murty B S | 2005 | Materials Science and Engineering A2005，393，12： | 1 |
| 6 | Proteomic profiling of high glucose primed monocytes identifies cyclophilin A as a potential secretory marker of inflammation in type 2 diabetes显示文摘 | Ramachandran S Venugopal A Sathisha K | 2012 | Proteomics2012，12，18： | 1 |
| 7 | Reeominat env-gp85 of HPRS103(subgroup J)avian leucosis virus: antigenitic charac- terization and usefulness as a diagnosis reagent显示文摘 | Venugopal K Howes K Ban'on | 1997 | Avian Dis1997，11，： | 1 |
| 8 | Intra-islet endothelial cell and β-cell crosstalk: Implication for islet cell transplantation显示文摘The intra-islet microvasculature is a critical interface between the blood and islet endocrine cells governing a number of cellular and pathophysiological processes associated with the pancreatic tissue. A growing body of evidence indicates a strong functional and physical interdependency of β-cells with endothelial cells(ECs), the building blocks of islet microvasculature. Intra-islet ECs, actively regulate vascular permeability and appear to play a role in fine-tuning blood glucose sensing and regulation. These cells also tend to behave as 'guardians', controlling the expression and movement of a number of important immune mediators, thereby strongly contributing to the physiology of islets. This review will focus on the molecular signalling and crosstalk between the intra-islet ECs and β-cells and how their relationship can be a potential target for intervention strategies in islet pathology and islet transplantation. | Siddharth Narayanan Gopalakrishnan Loganathan Maheswaran Dhanasekaran William Tucker Ankit Patel Venugopal Subhashree SriPrakash Mokshagundam Michael G Hughes Stuart K Williams Appakalai N Balamurugan | 2017 | World Journal of Transplantation2017，7，2： | 1 |
| 9 | Dynamic association rule mining using genetic algorithms 显示文摘 | Deepa Shenoy P Srinivasa K G Venugopal K R | 2005 | Intelligent Data Analysis2005，5，9： | 1 |
| 10 | Bulk preservation of small pelagic fish by gamma irradiation : studies on a model storage system using Anchovies 显示文摘 | Lakshmanan L Venugopal V Venketashvaran K | 1999 | Food Research International1999，32，10： | 1 |
| 11 | Authentication Using Finger Knuckle Prints 显示文摘 | Hegde C Shenoy P D Venugopal K R | 2013 | Signal Image mad Video Processing2013，7，4： | 1 |
| 12 | Yield maximization in cassava through a systematic approach in fertilizer use显示文摘 | John K S Venugopal V K Saraswathi P | | 0，，： | 1 |
| 13 | Antigenic variants of subgroup J avian leukosis virus: sequence analysis reveals multiple changes in the env gene显示文摘 | VENUGOPAL K SMITH L M HOWES K | 1998 | J Gen Virol1998，79，4： | 1 |
| 14 | Growth of tool wear in turning of Ti6Al4V alloy under cryogenic coohng显示文摘 | K A Venugopal S Paul A B Chattopadhyay | 2007 | Wear2007，262，： | 1 |
| 15 | Idiopathic fascicular ventricular tachycardia显示文摘 | Francis J Venugopal K Khadar SA | | 0，，03： | 1 |
| 16 | Vitellins and vitellogenins of Dysdercus koenigii (Heteroptera:Pyrrhocoridae)-identification,purification and temporal pattern显示文摘 | Venugopal K J Kumar D | 1999 | Comp Biochem Physiol:Part B1999，124，： | 1 |
| 17 | Osteogenic differentiation of human Wharton's jelly stem cells on nanofibrous substrates in vitro显示文摘 | Gauthaman K Venugopal JR Yee FC | | 0，，： | 1 |
| 18 | Growth of tool wear in turning of Ti-6A1-4V alloy under cryogenic cooling显示文摘 | VENUGOPAL K A PAUL S CHATTOPADHYAY A B | 2007 | Wear2007，262，910： | 1 |
| 19 | Instability map for cold and warm working of as-cast 304 stainless steel 显示文摘 | Venugopal S Mannan S L Prasad Y V R K | 1997 | Journal of Materials Processing Technolegy1997，65，： | 1 |
| 20 | Identification of optimum process parameters for hot extrusion using finite element simulation and processing maps 显示文摘 | SIVAPRASAD P V VENUGOPAL S DAVIES C H J PRASAD Y U R K | 2004 | Modelling and Simulation in Materials Science and Engineering2004，12，： | 1 |
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