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| 1 | Apoptotic activity of caged xanthones from Garcinia hanburyi in cholangiocarcinoma cell lines显示文摘AIM:To investigate the growth inhibitory mechanism of four caged xanthones from Garcinia hanburyi in cholangiocarcinoma(CCA) KKU-100 and KKU-M156 cells.METHODS:Four caged xanthones,selected on the basis of their anticancer potency and chemical structure diversities(i.e.isomorellin,isomorellinol,forbesione and gambogic acid) were used in this study.Growth inhibition of these caged xanthones was determined using the sulforhodamine B assay.Induction of apoptosis was assessed by observing cell morphology,ethidium bromide and acridine orange staining and DNA fragmentation assay.Levels of apoptotic-related gene and protein expressions were determined by a real-time reverse transcriptase polymerase chain reaction and Western blotting analysis,respectively.RESULTS:The compounds were found to inhibit growth of both cell lines in a dose-dependent manner and also showed selective cytotoxicity against the cancer cells when compared with normal peripheral blood mononuclear cells.Growth suppression by these compounds was due to apoptosis,as evidenced by the cell morphological changes,chromatin condensation,nuclear fragmentation,and DNA ladder formation.At the molecular level,these compounds induced down-regulation of Bcl-2 and survivin proteins with up-regulation of Bax and apoptosisinducing factor proteins,leading to the activation of caspase-9 and-3 and DNA fragmentation.The functional group variations did not appear to affect the anticancer activity with regard to the two CCA cell lines;however,at a mechanistic level,isomorellinol exhibited the highest potency in increasing the Bax/Bcl-2 protein expression ratio(120 and 41.4 for KKU-100 and KKU-M156,respectively) and in decreasing survivin protein expression(0.01 fold as compared to control cells in both cell lines).Other activities at the molecular level indicate that functional groups on the prenyl side chain may be important.CONCLUSION:Our findings for the first time demonstrate that four caged xanthones induce apoptosis in CCA cells which is mediated through a mitochondriadependent signaling pathway. | Chariya Hahnvajanawong Wongwarut Boonyanugomol Tapanawan Nasomyon Watcharin Loilome Nisana Namwat Natthinee Anantachoke Wichittra Tassaneeyakul Banchob Sripa Wises Namwat Vichai Reutrakul | 2010 | World Journal of Gastroenterology2010，16，18： | 5 |
| 2 | 水稻白叶枯病菌抗药性现象的室内研究显示文摘水稻白叶枯病是水稻的主要病害,目前我国防治该病的药剂主要是噻枯唑(川化—018),在含药含菌培养基上,筛选出了叶枯净(5—氧酚嗪)抗性菌,其突变率为2×10^(-6),抗性指数为333。未发现噻枯唑和渝—7802抗性菌。而在活体上直接喷药筛选时既发现了叶枯净抗性菌(抗性指数大于108),又发现了噻枯唑抗性菌(抗性指数大于196),仍然未发现渝—7802抗性菌,进一步的试验证明,噻枯唑活体抗性菌,离体测定时无抗药性,说明噻枯唑活体抗性和离体抗性不一致,而叶枯净的活体抗性和离体抗性一致。交互抗性测定证明,噻枯唑、叶枯净、渝—7802三者之间无交互抗性现象,而噻枯唑与敌枯双之间存在着强烈的正交互抗性。 | 王文相 顾江涛 夏静 Vichai Korpraditskul | 1998 | 植物保护学报1998，25，2： | 4 |
| 3 | Sulforhodamine B colorimetric assay for cytotoxicity screening显示文摘 | Vichai V Kirtikara K | 2006 | Nat Protoc2006，1，3： | 1 |
| 4 | Complete nucleotide sequence and genomic organization of hepatopancreatic parvovirus (HPV) of Penaeus monodon显示文摘 | Wasana S Pongsopee A Vichai B | 2006 | Virology2006，346，： | 1 |
| 5 | Sulforhodamine B colorimetric assay for cytotoxicity screening显示文摘 | VICHAI V KIRTIKARA K | 2006 | Nature Protocols2006，1，3： | 1 |
| 6 | Glucose-6-Phosphate Dehydrogenase Variants Associated with Favism in Thai Children显示文摘 | Vichai Laosombat Benjamas Sattayasevana Teerachit Chotsampancharoen Malai Wongchanchailert | 2006 | International Journal of Hematology2006，，2： | 1 |
| 7 | Novel cytotoxic 1 H -cyclopenta[ b ]benzofuran lignans from Aglaia elliptica显示文摘 | Baoliang Cui Heebyung Chai Thawatchai Santisuk Vichai Reutrakul Norman R. Farnsworth Geoffrey A. Cordell John M. Pezzuto A. Douglas Kinghorn | 1997 | Tetrahedron1997，，52： | 1 |
| 8 | Evaluation of the mutagenic, cytotoxic, and antitumor potential of triptolide, a highly oxygenated diterpene isolated from Tripterygium wilfordii显示文摘 | Lisa A. Shamon John M. Pezzuto Jewell M. Graves Rajeshwari R. Mehta Sirichai Wangcharoentrakul Ranee Sangsuwan Suttiporn Chaichana Patoomratana Tuchinda Per Cleason Vichai Reutrakul | 1997 | Cancer Letters1997，，1： | 1 |
| 9 | Why a proactive maintenance program for hydraulic oil is part of statistical process control 显示文摘 | Srimongkolkul Vichai | 1997 | Lubrication Engineering1997，53，4： | 1 |
| 10 | The anti-inflammatory effects and the inhibition of neutrophil responsiveness by Barleria lupulina and Clinacanthus nutans extracts显示文摘 | Payong Wanikiat Ampai Panthong Pacharawan Sujayanon Chalobon Yoosook Adriano G. Rossi Vichai Reutrakul | 2007 | Journal of Ethnopharmacology2007，，： | 1 |
| 11 | Sulforhodamine B colorimetric assay for cytotoxicity screening显示文摘 | Vichai V Kirtikara K | 2006 | Nat Protoc2006，1，3： | 1 |
| 12 | Digestive enzyme activities in grouper, Epinephelus coioides from first feeding through larva development显示文摘 | Vichai V Sunit R Poonsin P | 1997 | Sonkhla1997，1，： | 1 |
| 13 | Ondansetron versus metoclopramide in prophylaxis of nausea and vomiting for laparoscopic cholecystectomy: a prospective double-blind randomized study 显示文摘 | Trichak S Puttan T Vichai C | 2008 | Asian J Surg2008，31，2： | 1 |
| 14 | Pulmonary arterial hypertension in previously splenectomized patients with β-Thalassemic disorders显示文摘 | Vichai Atichartakarn Khanchit Likittanasombat Suporn Chuncharunee Pakorn Chandanamattha Surapon Worapongpaiboon Pantep Angchaisuksiri Katcharin Aryurachai | 2003 | International Journal of Hematology2003，，2： | 1 |
| 15 | Cytotoxic and anti - HIV - 1 constituents from leaves and twigs of Gardenia tubifera显示文摘 | Vichai Reutrakul Chongkon Krachangchaertg Patoomratana Tuchinda Manat Pohmakotr Thaworn Jaipetch Chalobon Yoosook Jittra Kasisit Samaisukh Sophasan d Kulawee Sujaritd Thawatchai Santisuk | 2004 | Tetrahedron2004，60，： | 1 |
| 16 | Fasciola gigantica : Anthelmintic effect of the aqueous extract of Artocarpus lakoocha显示文摘 | Naruwan Saowakon Tawewan Tansatit Chaitip Wanichanon Waraporn Chanakul Vichai Reutrakul Prasert Sobhon | 2009 | Experimental Parasitology2009，，4： | 1 |
| 17 | Sulforhodamine B colofimetric assay for cytotoxicity screening显示文摘 | Vichai V Kirtikara K | 2006 | Nat Protoe2006，1，3： | 1 |
| 18 | Sulforhodamine B colorimetric assay for cytotoxicity screening 显示文摘 | Vichai V Kirtikara K | 2006 | Nat Protoc2006，1，3： | 1 |
| 19 | Sulforhodamine B colorimetric assay for cytotoxicity screening显示文摘 | Vichai V Kirtikara K | 2006 | Nat Protoc2006，1，3： | 1 |
| 20 | Sulforhodamine B colorimetric assay for cytotoxicity screening显示文摘 | Vichai V Kirtikara K | 2006 | Nature Protocols2006，1，3： | 1 |
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