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| 1 | Effects of salvianolic acid-A on NIH/3T3 fibroblast proliferation,collagen synthesis and gene expression显示文摘AIM To investigate the mechanisms ofsalvianolic acid A(SA-A)against liver fibrosisin vitro.METHODS NIH/3T3 fibroblasts were culturedroutinely,and incubated with 10-4mol/L-10-7mol/L SA-A for 22 h.The cell viability wasassayed by[3H]proline incorporation,cellproliferation by[3H]TdR incorporation,cellcollagen synthetic rate was measured with[3H]proline impulse and collagenase digestionmethod.The total RNA was prepared from thecontrol cells and the drug treated cellsrespectively,and α(1)I pro-collagen mRNAexpression was semi-quantitatively analyzedwith RT-PCR.RESULTS 10-4mol/L SA-A decreased cellviability and exerted some cytotoxiciy,while10-5mol/L-10-7mol/L SA-A did not affect cellviability,but inhibited cell proliferationsignificantly,and 10-6mol/L SA-A had the besteffect on cell viability among theseconcentrations of drugs.10-5mol/L-10-6mol/LSA-A inhibited intracellular collagen syntheticrate,but no significant influence on extracellularcollagen secretion.Both 10-5mol/L and10-6mol/L SA-A could decrease α(1)I pro-collagen mRNA expression remarkably.CONCLUSION SA-A had potent action againstliver fibrosis.It inhibited NIH/3T3 fibroblastproliferation,intracellular collagen syntheticrate and type I pro-collagen gene expression,which may be one of the main mechanisms of thedrug. | Liu CH Hu YY Wang XL Liu P Xu LM | 2000 | World Journal of Gastroenterology2000，6，3： | 25 |
| 2 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 3 | Application of Nanopowder to High Temperature Single Crystal Fiber Sensor显示文摘ZrO2, CuO, Al2O3 and SiO2 mixed nanopowder was used to coat the head of sapphire single crystal fiber for improving the properties of high-temperature single crystal fiber sensor. The result indicates that the head coated with above mixed nanopowder shows better optical stability,shorter response time and higher thermal shock resistance, in comparison with the head coated with coarse-grained particles with the same chemical | Wang, YQ Wu, XJ Ye, LH Tong, LM Shen, YH | 1997 | Journal of Materials Science & Technology1997，13，4： | 3 |
| 4 | TSC1-mTOR-PLK轴以阶段特异性方式调节从施旺细胞增殖到髓鞘形成的内稳态开关显示文摘恰如其分的外周髓鞘形成取决于雪旺细胞增殖与分化进程间的平衡。丝氨酸/苏氨酸激酶(mTOR)整合多种环境因素,是细胞生长、代谢、发挥作用的中枢调节者。本文报道了一种mTOR的负性调节剂——结节性硬化复合体(TSC1),通过控制细胞增殖和髓鞘稳态,建立了雪旺细胞谱系进展和髓鞘形成的阶段依赖性程序。小鼠雪旺细胞祖细胞中TSC1的解离导致mTOR信号通路激活,继而导致雪旺细胞过量增殖,分化受阻,髓鞘形成减少。转录组分析显示,TSC1突变体中的mTOR活化使得polo样激酶(PLK)依赖性通路和细胞周期调节剂上调。弱化mTOR或者对PLK进行药理抑制部分挽救了因TSC1缺失导致的外周神经发育过程中的髓鞘形成减少。相较之下,成年小鼠成熟雪旺细胞中TSC1缺失可导致髓鞘的过度增殖和过度生长。本文的发现提示了TSC1-mTOR-PLK信号轴在控制雪旺细胞的发育过程中,从增殖到分化和髓鞘内稳态中起到的阶段特异性功能。 | Jiang M Rao R Wang J Wang J1 Xu L Wu LM Chan JR Wang H Lu QR 聂昊(编译) | 2018 | 神经损伤与功能重建2018，13，5： | 2 |
| 5 | Partitioning regularity of nonionic organic mixtures in organic phase/water system显示文摘The partitioning regularity of nonionic organic mixtures in organic phase/water system is revealed. The equation for calculating the partition coefficients of mixtures (KMD), together with the determination model, is derived from the equilibrium partitioning models (EPMs). Based on these derived equations, the KMD values of 20 mixtures containing halogenated benzenes are obtained. The results show that stronger hydrophobidty of an individual chemical in the mixture results in the stronger hydrophobidty of the mixture and the greater the proportion of this chemical, the stronger the hydrophobidty of the mixture will be. This partitioning regularity is helpful to the study of the toxicity for mixtures and the environmental behavior, such as transfer or accumulation, for mixed organic pollutants. | Lin, ZF Yu, HX Huang, LM Feng, JF Wang, LS | 2001 | Chinese Science Bulletin2001，46，17： | 2 |
| 6 | Effet of Maotai liguor in inducing metallothioneins and on hepatic stellate cells显示文摘 | Cheng ML Wu J Wang HQ Xue LM Tan YZ Liu P Li CX Huang NH Yao YM Ren LZ Ye L Li L Jia ML | 2001 | Shijie Huaren Xiaohua Zazhi2001，9，12： | 2 |
| 7 | Association between high-sensitivity cardiac troponin T and N-terminal pro-brain natriuretic peptide in a community based population显示文摘 | Xu RY Ye P Luo LM Sheng L Wu HM Xiao WK Zheng J Wang F Xiao TH | 2014 | Chinese Medical Journal2014，，4： | 2 |
| 8 | Surgical treatment of 263cases tuberculous of the chestwall显示文摘 | WANG SX WANG SL LI LM | 1988 | Chin J Surg1988，26，3： | 1 |
| 9 | Mono-L- aspartyl chlorin e6 (NPe6) and hematoporphyrin derivative (HpD) in photodynamic therapy administered to a human cholangiocarcinoma model 显示文摘 | Wong Kee Song LM Wang KK Zinsmeister AR | 1998 | Cancer1998，82，2： | 1 |
| 10 | microRNA-125 b inhibits cell migration and invasion by targeting matrix metallopeptidase 13 in bladder cancer显示文摘 | Wu DY Ding J J Wang LM | 2013 | Oncology letters2013，5，3： | 1 |
| 11 | Prevalence and control of dia-betes in Chinese adults 显示文摘 | Xu Y Wang LM He J | 2013 | J Am Med Assoc2013，310，9： | 1 |
| 12 | Prevalence and control of diabetes in Chinese adults显示文摘 | Xu Y Wang LM He J | 2013 | JAMA2013，310，9： | 1 |
| 13 | The epidemiology of thyroid disease and implications for screening显示文摘 | Wang C Crapo LM | 1997 | Endocrinol Metab Clin North Am1997，26，1： | 1 |
| 14 | 388 EKB-569: a new irreversible inhibitor of epidermal growth factor receptor tyrsine kinase for the treatment of cancer显示文摘 | Discafani C Wang YF Tsou HR Overbeek EG Nilakantan R | 2000 | Clin Cancer Res2000，，： | 1 |
| 15 | Exogenous biliver- din ameliorates ischemia-reperfusion injury in small-for- size rat liver grafts显示文摘 | Tang LM Wang YP Wang K | 2007 | Transplant Proc2007，39，： | 1 |
| 16 | MMP and TIMP expres-sion in quiescent,dividing,and differentiating human lens cells显示文摘 | Hodgkinson LM Duncan G Wang L | 2007 | Invest Ophthalmol Vis Sci2007，48，9： | 1 |
| 17 | Association between HLA-B * 1502 allele and carbamazepine- induced severe cutaneous adverse reactions in Han people of southern China mainland 显示文摘 | Wang Q Zhou JQ Zhou LM | 2011 | Seizure2011，20，6： | 1 |
| 18 | A new species of freshwater crab (Decapoda : Brachyura : Potamidae) from Dong Island, Matsu, Taiwan, defined by morphological and molecular characters, with notes on its biogeography 显示文摘 | Shih HT Chen GX Wang LM | 2005 | J Nat History2005，59，31： | 1 |
| 19 | Comparative analysis of a large dataset indicates that internal transcribed spacer(ITS)should be incorporated into the core barcode for seed plants显示文摘 | Li DZ Gao LM Li H Wang H Ge X J Liu JQ Chen ZD Zhou SL Chen SL Yang JB | | 0，，： | 1 |
| 20 | A vegetable- fruit-soy dietary pattern protects against breast cancer among postmenopausal Singapore Chinese women 显示文摘 | Butler LM Wu AH Wang R | 2010 | Am J Clin Nutr2010，91，4： | 1 |
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