| 1 | Development and Validation of a Novel Reverse Phase HPLC for Determination of Aloperine in Aloperine Tablets显示文摘Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and antimflammatory actions. Methods The samples were analyzed on a ODS column with methanol-water-triethylamine (3:97:0.1 V/V) as a mobile phase, The flow rate was 1.0 mL·min^-1, and UV detection wavelength 205 nm. Results Linear regression equation was A = 1.6920C + 1.7455 (r^2 = 0.9999, n = 5) in concentratim ranging from 20 to 120μg·mL^-1. The recoveries were 101.2±1.46 % at 80 μg·mL^-1, 100.5±0.75% at 100μg·mL^-1, and 100.7±1.10% at 120 μg·mL^-1, respectively, and the precisions of aloperine within or between run were from 0.80% to 1.98% ( n = 5). The relative contents of aloperine in three lots of tablets were 101.59 ± 1.38%, 98.46 ± 0.23%, and 99.41 ± 1.09% ( n = 3).Conclusion The newly developed reverse phase HPLC method is simple and useful for daily assay of aloperine tablets and can overcome the interference from excipient and other alkaloids in titration and UV detection. | WANGHua ZHENGLi WANGZhan ZHANGYong-qiang] WUFeng-lan | 2003 | Journal of Chinese Pharmaceutical Sciences2003，12，4： | 4 |
| 3 | H3, An Acidic Polysaccharide from Cuscuta chinensis显示文摘H3, a homogeneous acidic polysaccharide was obtained from the seeds of Cuscuta chinensisLam. Its structure was characterized for the first time by chemical and spectroscopic methods to be a highly branchedheteropolysaccharide with mean molecular weight of more than lxl0^6. It was composed of 1,6-1inked-β-D Galp, 1,4-linked-β-D Galp, 1,4-1inked-β-D GalA, 1,3,6-1inked-β-D Galp and 1,2,4-1inked Rhap, with branching points at O-2or 0-4 of 1,2,4-1inked Rhap and 0-3 of 1,3,6-1inked-β-D Galp. Its side chains included 1-1inked Araf, 1,5-1inked Arafand 1,3,5-1inked Araf at O-3 of 1,6-1inked Galp in the main chain. | WangZhan LiuCuiping FangJinian | 2001 | Journal of Chinese Pharmaceutical Sciences2001，10，2： | 1 |
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