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| 1 | Expression of NF-кB and human telomerase reverse transcriptase in gastric cancer and precancerous lesions显示文摘AIM:To investigate the expression of NF-κBp65 protein and human telomerase reverse transcriptase (hTERT) and their correlation in gastric cancer and precancerous lesions.METHODS: Forty-one patients with primary gastric cancer,15 with dysplasia, 23 intestinal metaplasia and 10 with normal gastric mucosa were included in this study.Expression of NF-κBp65 protein,hTERT mRNA and protein were determined by immunohistochemistry and in situ hybridization.RESULTS:The rate of p65 expression in normal gastric mucosa, intestinal metaplasia,dysplasia and carcinoma was 0%, 34.78%, 53.33% and 60.98%,respectively,while the rate of hTERT mRNA expression was 10.00%,39.13%,66.67% and 85.37% and the rate of hTERT protein expression was 0%,30.43%,60.00% and 78.05%,respectively.All the three parameters were significantly increased in dysplasia and carcinoma compared to normal mucosa, while the expression levels were also significantly higher in carcinoma than in intestinal metaplasia (P<0.05).In gastric cancer tissues, nuclear staining rates of p65 and hTERT protein were both significantly associated with the degree of differentiation, lymph node metastasis,clinical stage and invasion depth (P<0.05).However,hTERT mRNA expression was only significantly associated with clinical stage.There was a positive correlation between p65 and hTERT mRNA(rs=0.661-0.752, P<0.01),and between hTERT protein and hTERT mRNA (rs=0.609-0.750, P<0.01).CONCLUSION: NF-κBp65 and hTERT expressions are upregulated at the early stage of gastric carcinogenesis.NF-κB activation may contribute to hTERT expression and thereby enhance telomerase activity, which represents an important step in carcinogenesis progress. | WeiWang He-ShengLuo Bao-PingYu | 2004 | World Journal of Gastroenterology2004，10，2： | 30 |
| 2 | Expressions of cysteine-rich61,connective tissue growth factor and Nov genes in hepatocellular carcinoma and their clinical significance显示文摘AIM: To investigate the expression of cysteine-rich61 (Cyr61),connective tissue growth factor (CTGF) and nephroblastomaoverexpressecl gene (Nov) in hepatocellular carcinoma (HCC),and to evaluate the relationship between Cyr61, CTGF and Nov genes expression with invasion and metastasis of HCC.METHODS: Thirty-one HCC specimens were divided into small hepatocellular carcinoma (SHCC), nodular hepatocellular carcinoma (NHCC), solitary large hepatocellular carcinoma (SLHCC) according to their diameter and number of nodes. Reverse transcription polymerse chain reaction (RT-PCR) was used to detect the mRNA expression levels of Cyr61, CTGF and Nov genes in 31 resected specimens of hepatocellular carcinoma and para-cancerous normal liver tissues semi-quantitatively and the relation between their expression levels and clinical pathological parameters were compared.RESULTS: The expressions of Cyr61 and CTGF mRNA in carcinoma tissues were significantly higher than those in para-cancerous normal liver tissues (P<0.01). The expressions of Cyr61 and CTGF mRNA in HCC with venous invasion were higher than those in HCC without venous invasion. CTGF expression in HCC Edmondson's grade Ⅲ-IV was significantly higher than that in HCC Edmondson's grade I-II (P = 0.022). There was no obvious correlation between Nov mRNA and clinical-pathological features.Compared to NHCC, SLHCC had better cell differentiation,easier capsule formation, less microscopic venous invasion,milder liver cirrhosis. The expressions of Cyr61 and CTGF mRNA in NHCC were significantly higher than those in SLHCC and SHCC.CONCLUSION: Cyr61 and CTGF genes may play an important role in hepatocellular carcinogenesis and correlate with recurrence and metastasis of hepatocellular carcinoma.SLHCC has better biological behaviors than NHCC. | Zhi-JunZeng Lian-YueYang XiangDing WeiWang | 2004 | World Journal of Gastroenterology2004，10，23： | 28 |
| 3 | Identification of effective siRNA against K-ras in human pancreatic cancer cell line MiaPaCa-2 by siRNA expression cassette显示文摘AIM: We shall construct the small interfering RNA (siRNA)expression cassette (SEC) targeting activated K-ras gene sequence, identify more effective siRNA sequence against K-ras gene in human pancreatic cancer cell line MiaPaCa-2 by SEC and reveal the anti-cancer effects of RNA interference (RNAi) and its therapeutic possibilities.WETHODS: Three different sites of SECs were constructed by PCR. K1/siRNA, K2/siRNA and K3/siRNA are located at sites 194, 491 and 327, respectively. They were transfected into MiaPaCa-2 cells by liposome to inhibit the expression of activated K-ras. In the interfering groups of sites 194 and 491, we detected the apoptosis in cells by FACS after they were incubated for 48 h, then we tested the alternation of K-ras gene in MiaPaCa-2 cells by RT-PCR immunofluorescence, respectively.RESULTS: Introduction of the K1/siRNA and K2/siRNA against K-ras into MiaPaCa-2 cells leads to increased apoptosis, and the number of apoptotic cells is increased compared with control cells. The tests of RT-PCR immunofluorescence show the effects of inhibiting expression of activated K-ras gene by RNA interference in the K1/siRNA and K2/siRNA groups. We also find that the introduction of K3/siRNA has no effect on MiaPaCa-2 cells.CONCLUSION: K1/siRNA and K2/siRNA can inhibit the expression of activated K-fas but K3/siRNA has no effect,demonstrating that K1/siRNA and K2/siRNA are effective sequences against K-ras gene and K3/siRNA are not. We conclude that specific siRNA against K-ras expression may be a powerful tool to be used therapeutically against human pancreatic cancer. | WeiWang Chun-YouWang Ju-HuaDong XiongChen MinZhang GangZhao | 2005 | World Journal of Gastroenterology2005，11，13： | 20 |
| 4 | Mechanical and Wear Properties of Nanostructured Surface Layer in Iron Induced by Surface Mechanical Attrition Treatment显示文摘A porosity-free and contamination-free surface layer with grain sizes ranging from nanometer to micrometer in Fe samples was obtained by surface mechanical attrition treatment (SMAT) technique. Mechanical and wear properties of the surface layer in the SMATed and annealed Fe samples were measured by means of nanoindentation and nanoscratch tests, respectively. Experimental results showed that the hardness of the surface layer in the SMATed Fe sample increased evidently due to the grain refinement. The elastic noduli of the surface layers in the SMATed and annealed Fe samples were unchanged, independent of grain size in the present grain size regime. Compared with the original Fe sample, the wear resistance enhanced and the coefficient of friction decreased in the surface layer of the SMATed Fe sample. | NairongTAO WeipingTONG ZhenboWANG WeiWANG ManlingSUI JianLU KeLU | 2003 | Journal of Materials Science & Technology2003，19，6： | 17 |
| 5 | Nitroester drug's effects and their antagonistic effects against morphine on human sphincter of Oddi motility显示文摘AIM: To evaluate the effects of nitroester drugs on human sphincter of Oddi (SO) motility and their antagonistic effects against morphine which shows excitatory effect on Oddi's sphincter motility.METHODS: The effects of these drugs on SO were evaluated by means of choledochofiberoscopy manometry.A total of 67 patients having T-tubes after cholecystectomy and choledochotomy were involved in the study, they were randomly divided into glyceryl trinitrate (GTN) group,isosorbide dinitrate (ISDN) group, pentaerythritol tetranitrate (PTN) group, morphine associated with GTN group, morphine associated with ISDN group and morphine associated with PTN group. Basal pressure of Oddi's sphincter (BPOS), amplitude of phasic contractions (SOCA), frequency of phasic contractions (SOF), duration of phasic contractions (SOD), duodenal pressure (DP) and common bile duct pressure (CBDP) were scored and analyzed. Morphine was given intramuscularly while nitroester drugs were applied sublingually.RESULTS: BPOS and SOCA decreased significantly after administration of ISDN and GTN, BPOS reduced from 10.95±7.49 mmHg to 5.92±4.04 mmHg (P<0.05) evidently after application of PTN. BPOS increased from 7.37±5.58mmHg to 16.60±13.87 mmHg, SOCA increased from 54.09±38.37 mmHg to 100.70±43.51 mmHg, SOF increased from 7.15±3.20 mmHg to 10.38±2.93 mmHg and CBDP increased 3.75±1.95 mmHg to 10.49±8.21 mmHg (P<0.01)evidently after injection of morphine. After associated application of ISDN and GTN, the four indications above decreased obviously. As for application associated with PTN,SOCA and SOF decreased separately from 100.64±44.99mmHg to 66.17±35.88 mmHg and from 10.70±2.76 mmHg to 9.04±1.71 mmHg (P<0.05) markedly.CONCLUSION: The regular dose of GTN, ISDN and PTN showed inhibitory effect on SO motility, morphine showed excitatory effect on SO while GTN, ISDN and PTN could antagonize the effect of morphine. Among the three nitroester drugs, the effect of ISDN on SO was most significant. | Shuo-DongWu Zhen-HaiZhang Dong-YanLi Jun-ZheJin JingKong ZhongTian WeiWang Min-FeiWang | 2005 | World Journal of Gastroenterology2005，11，15： | 16 |
| 6 | Effects of narcotic analgesic drugs on human Oddi's sphincter motility显示文摘AIM: To assess the effects of intramuscular analgesics(morphine, Ap-237, pethidine and tramadol) on humanOddi's sphincter motility with choledochoscope manometry.METHODS: A total of 70 patients having T tubes aftercholecystectomy and choledochotomy were assessed bycholedochoscope manometry. They were randomly dividedinto morphine group, Ap-237 group, pethidine group andtramadol group. Basal pressure of Oddi's sphincter (BPOS),amplitude of phasic contractions (SOCA), frequency ofphasic contractions (SOF), duration of phasic contractions(SOD), duodenal pressure (DP) and common bile ductpressure (CBDP) were scored and analyzed. All narcoticanalgesic drugs were administered intramuscularly.RESULTS: Levels of BPOS, SOCA and SOF were increasedafter injection of morphine and Ap-237 (P<0.05), level of CBDPwas increased from 4.97+3.87 mmHg to 8.62+7.43 mmHg(10 min later) and 7.32+5.95 mmHg (20 min later) afterinjection of morphine (P<0.01). No apparent changeoccurred after intramuscular injection of pethidine. Level ofBPOS was increased from 7.01+5.50 mmHg to 2.87+2.78 mmHg10 min after injection of tramadol and SOCA was decreasedfrom 63.34+35.29 mmHg to 45.90+27.86 mmHg (10 minlater, P<0.05) and 35.97+24.30 (20 min later, P<0.01) afteradministration of tramadol.CONCLUSION: All these findings indicate that Oddi'ssphincter manometry via choledochoscope is a practicaland new way to study the dynamics of Oddi' s sphincter.The regular dose of morphine and Ap-237 could increaseBPOS, SOF and SOCA. Morphine could increase the levelof CBDP, demonstrating an excitatory effect on the sphincterof Oddi. Pethidine had no effect on Oddi's sphincter motility.Tramadol shows an inhibitory effect on the motility of thesphincter of Oddi and decreases levels of BPOS and SOCA. | Shuo-DongWu Zhen-HaiZhang Jun-ZheJin JingKong WeiWang QiangZhang Dong-YanLi Min-FeiWang | 2004 | World Journal of Gastroenterology2004，10，19： | 15 |
| 7 | Role of AFP mRNA expression in peripheral blood as a predictor for postsurgical recurrence of hepatocellular carcinoma: A systematic review and meta-analysis显示文摘AIM: To identify the role of alpha-fetoprotein (AFP) mRNA expression in peripheral blood one week after surgery as a predictor for recurrence of hepatocellular carcinoma (HCC).METHODS: Published studies fulfilling the selection criteria were identified by searching several databases online. After a methodology assessment using a quality scale designed by European Lung Cancer Working Party, data in each research were aggregated by means of metaanalysis.RESULTS: Altogether 368 cases were included in the 9 selected studies, which fulfilled the selection criteria. The quality scores ranged from 35% to 84% with a median score of 55%. The 'design' subscore had the lowest median value (38%). By aggregating the data, a high χ2value (77.576) was presented. The fail-safe number was 136 and 64 for P = 0.05 and 0.01, respectively. CONCLUSION: AFP mRNA expression in peripheral blood 1 wk after surgery correlated with the recurrence of HCC and was a good predictor for tumor recurrence. | XiangDing Lian-YueYang Geng-WenHuang Jian-QingYang He-LiLiu WeiWang Ji-XiangPeng Jie-QuanYang Yi-MingTao Zhi-GangChang Xiu-ShouLing | 2005 | World Journal of Gastroenterology2005，11，17： | 15 |
| 8 | Expression of HIF-2α/EPAS1 in hepatocellular carcinoma显示文摘AIM:To investigate the expression of hypoxia-inducible factor (HIF)-2α/endothelial PAS domain protein1 (EPAS1) in hepatocellular carcinoma (HCC).METHODS: Expression of HIF-2α/EPAS1 was investigated immunohistochemically on paraffin-embedded sections from 97 patients with HCC.To further confirm that HIF-2α/EPAS1 in HCC tissues also correlated with angiogenesis, a parallel immunohistchemistry study of vascular endothelial growth factor (VEGF) was performed on these 97 cases.RESULTS:HIF-2α/EPAS1 could be detected in 50 of 97 cases (51.6%), including 19 weakly positive (19.8%), and 31 strongly positive (31.1%), the other 47 cases were negative(48.4%). The expression of HIF-2α/EPASlwas significantly correlated with tumor size,capsule infiltration, portal vein invasion, and necrosis. A parallel immunohistochemical analysis of VEGF demonstrated its positive correlation with capsule infiltration, portal vein invasion, and HIF-α/EPAS1 overexpression, which supported the correlation of HIF-2α/EPASlup-regulation with tumor angiogenesis. No apparent correlation was observed between HIF-2α/EPAS1 and capsular formation, presence of cirrhosis, and histological grade.CONCLUSION: HIF-2α/EPAS1 is expressed in most of HCC with capsular infiltration and portal vein invasion, which indicates a possible role of HIF-2α/EPAS1 in HCC metastasis. | GassimouBangoura Lian-YueYang Gen-WenHuang WeiWang | 2004 | World Journal of Gastroenterology2004，10，4： | 12 |
| 9 | Protective effect of nitric oxide induced by ischemic preconditioning on reperfusion injury of rat liver graft显示文摘AIM: Ischemic preconditioning (IP) is a brief ischemic episode,which confers a state of protection against the subsequent long-term ischemia-reperfusion injuries. However, little is known regarding the use of IP before the sustained cold storage and liver transplantation. The present study was designed to evaluate the protective effect of IP on the long-term preservation of liver graft and the prolonged anhepatic-phase injury.METHODS: Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation. All livers underwent 10 min of ischemia followed by 10 min of reperfusion before harvest. Rat liver transplantation was performed with the portal vein clamped for 25 min. Tolerance of transplanted liver to the reperfusion injury and liver damage were investigated. The changes in adenosine concentration in hepatic tissue and those of nitric oxide (NO) and tumor necrosis factor (TNF) in serum were also assessed.RESULTS: Recipients with IP significantly improved their one-week survival rate and liver function, they had increased levels of circulating NO and hepatic adenosine, and a reduced level of serum TNF, as compared to controls. Histological changes indicating hepatic injuries appeared improved in the IP group compared with those in control group. The protective effect of IP was also obtained by administration of adenosine,while blockage of the NO pathway using Nco-nitro-L-arginine methyl ester abolished the protective effect of IP.CONCLUSION: IP appears to have a protective effect on the long-term preservation of liver graft and the prolonged anhepatic-phase injuries. NO may be involved in this process. | Jian-PingGong BingTu WeiWang YongPeng Shou-BaiLi Lu-NanYan | 2004 | World Journal of Gastroenterology2004，10，1： | 10 |
| 10 | NF-κB activation and zinc finger protein A20 expression in mature dendritic cells derived from liver allografts undergoing acute rejection显示文摘AIM: To investigate the role of NF-κB activation and zinc finger protein A20 expression in the regulation of maturation of dendritic cells (DCs) derived from liver allografts undergoing acute rejection.
METHODS: Sixty donor male SD rats and sixty recipient male LEW rats weighing 220-300 g were randomly divided into whole liver transplantation group and partial liver transplantation group. Allogeneic (SD rat to LEW rat) whole and 50 % partial liver transplantation were performed. DCs from liver grafts 0 hour and 4 days after transplantation were isolated and propagated in the presence of GM-CSF in vitro. Morphological characteristics and phenotypical features of DCs propagated for 10 days were analyzed by electron microscopy and flow cytometry, respectively. NF-κB binding activity, IL-12p70 protein and zinc finger protein A20expression in these DCs were measured by EMSA and Western blotting, respectively. Histological grading of rejection was determined.
RESULTS: Allogeneic whole liver grafts showed no signs of rejection on day 4 after the transplantation. In contrast,allogeneic partial liver grafts demonstrated moderate to severe rejection on day 4 after the transplantation. After propagation for 10 days in the presence of GM-CSF in vitro,DCs from allogeneic whole liver grafts exhibited features of immature DC with absence of CD40 surface expression,these DCs were found to exhibit detectable but very low level of NF-κB activity, IL-12 p70 protein and zinc finger protein A20 expression. Whereas, DCs from allogeneic partial liver graft 4 days after transplantation displayed features of mature DC, with high level of CD40 surface expression, and as a consequence, higher expression of IL-12p70 protein, higher activities of NF-κB and higher expression of zinc finger protein A20 compared with those of DCs from whole liver grafts (P<0.001).
CONCLUSION: These results suggest that A20expression is up-regulated in response to NF-κB activation in mature DCs derived from allogeneic liver grafts undergoing acute rejection. Given the NF-κB inhibition function of this gene, it is suggested that their expression survives to limit NF-κB activation and maturation of DCs,and consequently inhibits the acute rejection and induces acceptance of liver graft. | Ming-QingXu WeiWang LanXue Lv-NanYan | 2003 | World Journal of Gastroenterology2003，9，6： | 8 |
| 11 | Effect of cisapride on intestinal bacterial and endotoxin translocation in cirrhosis显示文摘AIM: To investigate the effects of cisapride on intestinal bacterial overgrowth (IBO), bacterial and endotoxin translocation, intestinal transit and permeability in cirrhotic rats.METHODS: All animals were assessed with variables including bacterial and endotoxin translocation, intestinal bacterial overgrowth, intestinal transit and permeability.Bacterial translocation (BT) was assessed by bacterial culture of MLN, liver and spleen, IBO by a jejunal bacterial count of the specific organism, intestinal permeability by determination of the 24-hour urinary 99mTc-DTPA excretion and intestinal transit by measurement of the distribution of 51Cr in the intestine.RESULTS: Bacterial translocation (BT) and IBO was found in 48 % and 80 % cirrhotic rats respectively and none in control rats. Urinary excretion of 99mTc-DTPA in cirrhotic rats with BT (22.2±7.8) was greater than these without BT (10.5±2.9). Intestinal transit (geometric center ratio) was significantly delayed in cirrhotic rats (0.31±0.06) and further more delayed in cirrhotic rats with BT (0.24±0.06) than these without BT (0.38±0.11). Cirrhotic rats with IBO had significantly higher rates of intestinal bacterial and endotoxin translocation, slower intestinal transit time and higher intestinal permeability than those without IBO. It was also found that BT was closely associated with IBO and the injury of intestinal barrier. Compared with the placebo group,cisapride-treated rats had lower rates of bacterial/endotoxin translocation and IBO, which was closely associated with increased intestinal transit and improved intestinal permeability by cisapride.CONCLUSION: These results indicate that endotoxin and bacterial translocation in cirrhotic rats may be attributed to IBO and increased intestinal permeability. Cisapride that accelerates intestinal transit and improve intestinal permeability might be helpful in preventing intestinal bacterial and endotoxin translocation. | Shun-CaiZhang WeiWang Wei-YingRen Bo-MingHe KangZhou Wu-NanZhu | 2003 | World Journal of Gastroenterology2003，9，3： | 7 |
| 12 | Expression pattern of epithelial cell adhesion molecule on normal and malignant colon tissues显示文摘AIM: To investigate the expression pattern of epithelial cell adhesion molecule (Ep-CAM) on normal and malignant colon tissues to evaluate its diagnostic and therapeutic significance.METHODS: cDNA encoding Ep-CAv extracellular domain was cloned by reverse transcription-polymerase chain reaction (RT-PCR) from excised malignant colon tissues and inserted into a glutathione S-transferase (GST)-tagged vector. EpCAM-GST fusion protein was induced by isopropyl-β-D-thiogalactopyranoside (IPTG) and purified with glutathionesepharose. The Ep-CAM-GST fusion protein was mixed with Freund's adjuvant and Balb/c mice were immunized with it. Sp2/0 myeloma cells were fused with the spleen cells of the immunized mice. After having selected by indirect ELISA, the anti-Ep-CAM monoclonal antibodies (NAbs) were generated and the corresponding ascites were obtained.Finally, the human colon carcinoma tissue array prepared from seventy individual patients was stained with the antiEp-CAM NAbs.RESULTS: The isolated Ep-CAM cDNA sequence was identical to the data in GenBank. The expressed fusion protein was almost soluble and had a molecular weight (NW) of 53 ku.Four NAbs against Ep-CAM were obtained and designated as FMU-Ep1, FMU-Ep2, FMU-Ep3 and FMU-Ep4 respectively.Among them, FMU-Ep4 could recognize the natural EpCAM on Colo205 and SW480 cells, and all of them could be used for immunohistochemical staining of tissue sections.It was found that Ep-CAM was distributed differently in normal and various malignant colon tissues, including squamous cell carcinoma, signet-ring cell carcinoma and adenocarcinoma.In normal colon gland epithelia, Ep-CAM antigen was mainly distributed on the basolateral membrane and in the region between the basolateral membrane and the cytoplastic part near the nuclei, whereas the expression pattern of colon malignancies was mainly on the whole surface of epithelia and the expression was much higher than the normal colon tissues. The staining pattern of tissue array showed in adenocarcinoma and papillary adenocarcinoma, and the expression of Ep-CAM was increased from grade Ⅰ to grade Ⅲ.CONCLUSION: NAbs against Ep-CAM might be useful for research on the structure and function of Ep-CAM and may have diagnostic and therapeutic value to various colon carcinomas. | XinXie Chun-YanWang Yun-XinCao WeiWang RanZhuang Li-HuaChen Na-NaDang LiangFang Bo-QuanJin | 2005 | World Journal of Gastroenterology2005，11，3： | 7 |
| 13 | Cloning and expression of ornithine decarboxylase gene from human colorectal carcinoma显示文摘AIM: To construct and express ODC recombinant gene for further exploring its potential use in early diagnosis of colorectal carcinoma.METHODS: Total RNA was extracted from colon cancer tissues and amplified by reverse-transcription PCR with two primers, which span the whole coding region of ODC. The synthesized ODC cDNA was cloned into vector pQE-30 at restriction sites BamH I and Sal I which constituted recombinant expression plasmid pQE30-ODC. The sequence of inserted fragment was confirmed by DNA sequencing,the fusion protein including 6His-tag was facilitated for purification by Ni-NTA chromatographic column.RESULTS: ODC expression vector was constructed and confirmed with restriction enzyme digestion and subsequent DNA sequencing. The DNA sequence matching on NCBI Blast showed 99 % affinity. The vector was transformed into E.coli M15 and expressed. The expressed ODC protein was verified with Western blotting.CONCLUSION: The ODC prokaryote expression vector is constructed and thus greatly facilitates to study the role of ODC in colorectal carcinoma. | Hai-YanHu Xian-XiLiu Chun-YingJiang YanZhang Ji-FengBian YiLu ZhaoGeng Shi-LianLiu Chuan-HuaLiu Xiao-MingWang WeiWang | 2003 | World Journal of Gastroenterology2003，9，4： | 7 |
| 14 | CHOOSING STRUCTURE-DEPENDENT DRAG COEFFICIENT IN MODELING GAS-SOLID TWO-PHASE FLOW显示文摘IntroductionGas-solid two-phase flow is often encountered in chemical reactors for the process industry. For industrial users, design, scale-up, control and optimization for these reactors require a good understanding of the hydrodynamics of gas-solid two-phase flow. For researchers, exploration and prediction of the complex phenomena call for a good comprehension of the heterogeneous structure and of the dominant mechanisms of gas-solid and solid-solid interactions. | NingYang WeiWang WeiGe JinghaiLi | 2003 | China Particuology2003，1，1： | 7 |
| 15 | Recent Progress on Selected Topics in Database Research-A Report by Nine Young Chinese Researchers Working in the United States显示文摘The study on database technologies, or more generally, the technologies of dataand information management, is an important and active research field. Recently, many excitingresults have been reported. In this fast growing field, Chinese researchers play more and more activeroles. Research papers from Chinese scholars, both in China and abroad, appear in prestigious academic forums.In this paper, we, nine young Chinese researchers working in the United States, presentconcise surveys and report our recent progress on the selected fields that we are working on.Although the paper covers only a small number of topics and the selection of the topics is far frombalanced, we hope that such an effort would attract more and more researchers, especially thosein China, to enter the frontiers of database research and promote collaborations. For the obviousreason, the authors are listed alphabetically, while the sections are arranged in the order of theauthor list. | ZhiyuanChen ChenLi JianPei YufeiTao HaixunWang WeiWang JiongYang JunYang DonghuiZhang | 2003 | Journal of Computer Science & Technology2003，18，5： | 6 |
| 16 | Analysis of Galileo signal-in-space range error and positioning performance during 2015-2018显示文摘A long-term analysis of signal-in-space range error (SISRE) is presented for all healthy Galileo satellites, and the first pair of full operational capability satellites in wrong elliptical orbits. Both orbit and clock errors for Galileo show an obvious convergence trend over time. The annual statistical analyses show that the average root mean squares (RMSs) of SISRE for the Galileo constellation are 0.58 m (2015), 0.29 m (2016), 0.23 m (2017), and 0.22 m (2018). Currently, the accuracy of the Galileo signal-in-space is superior to that of the global positioning system (GPS) Block IIF (0.35 m). In addition, the orbit error accounts for the majority of Galileo SISRE, while the clock error accounts for approximately one-third of SISRE due to the high stability of the onboard atomic clock. Single point positioning results show that Galileo achieves an accuracy of 2-3 m, which is comparable to that of GPS despite the smaller number of satellites and worse geometry. Interestingly, the vertical accuracy of Galileo, which uses the NeQuick ionospheric model, is higher than that of GPS. Positioning with single frequency E1 and E5 show a higher precision than E5a and E5b signals. Regarding precise point positioning (PPP), the results indicate that a comparable positioning accuracy can be achieved among different stations with the current Galileo constellation. For static PPP, the RMS values of Galileo-only solutions are within 1 cm horizontally, and the vertical RMSs are mostly within 2 cm horizontally. For kinematic PPP, the RMSs of Galileo-only solutions are mostly within 4 cm horizontally and 6 cm vertically. | Weiwang Wu Fei Guo Jiazhu Zheng | 2020 | Satellite Navigation2020，1，1： | 6 |
| 17 | ADAM17 mRNA expression and pathological features of hepatocellular carcinoma显示文摘AIM: To study the expression of a disintegrin and metalloproteinase 17 (ADAM17) mRNA in hepatocellular carcinoma (HCC) and to evaluate the relationship between ADAM17 mRNA expression and clinicopathological featuresof HCC.METHODS: Hepatocellular carcinomas (HCC) from 31 cases were divided into small HCC (SHCC), nodular HCC (NHCC)and solitary large HCC (SLHCC) according to tumor diameter and the number of nodes. ADAM17 mRNA expressions were compared among those groups by means of semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).The relationship between ADAM17 mRNA expression level and clinicopathological features of HCC was evaluated.RESULTS: NHCC had lower differentiation and was more frequently of microvascular invasion (10/12) than SHCC(3/11) and SLHCC (3/8) (P<0.05), but no statistical difference was observed between SHCC and SLHCC comparing their clinicopathological features. ADAM17 mRNA expression was detected in 77.4% (24/31) of HCC tissues and was significantly higher than that in paired non-cancerous liver tissues in which only 35.5% (11/31) of the samples were detected of the expression (P<0.05). The expression of ADAM17 mRNA was much higher in NHCC than in SHCC and SLHCC(P<0.05), while no significant difference was discovered between SHCC and SLHCC. The quantities of ADAM17 mRNA were significantly higher in poorly differentiated HCC than in well or moderately differentiated HCC, but no statistical difference was found concerning liver cirrhosis,tumor capsule formation or microvascular invasion of the cancer.CONCLUSION: The increased expression of ADAM17 may play a key role in the development of HCC. The expression levels of ADAM 17 mRNA varied among different pathological types of HCC. Lower mRNA expression of ADAM17 mRNA in SLHCC may be associated with the better molecular pathological features of SLHCC. | XiangDing Lian-YueYang Gen-WenHuang WeiWang Wei-QunLu | 2004 | World Journal of Gastroenterology2004，10，18： | 5 |
| 18 | Differentially expressed genes between solitary large hepatocellular carcinoma and nodular hepatocellular carcinoma显示文摘AIM: To study the difference in gene expression between solitary large hepatocellular carcinoma (SLHCC) and nodular hepatocellular carcinoma (NHCC).METHODS: Polymerase chain reaction (PCR) products of 8464 human genes were spotted on a chip in array. DNAs were then fixed on a glass plate. Total RNA was isolated from freshly excised human SLHCC (n = 7) and NHCC (n = 15) tissues, and was reversely transcribed to cDNAs with the incorporation of fluorescent dUTP for preparation of hybridization probes. The mixed probes were then hybridized to the cDNA microarray. After highly stringent washing,cDNA microarray was scanned for the fluorescent signals to display the difference between the two kinds of HCC. In addition, the expression of RhoC and protocadherin LKC was also detected with the reverse transcriptase polymerase chain reaction (RT-PCR) method.RESULTS: Among the 8 464 human genes, 668 (7.89%) genes were expressed differentially at the mRNA levels between SLHCC and NHCC. Three hundred and fifty five (4.19%) genes, including protocadherin LKC, were upregulated, whereas 313 (3.70%) genes, including RhoC,were down-regulated. The mRNA expression levels of RhoC and protocadherin LKCwere confirmed by RT-PCR. Analysis of differentially expressed genes confirmed that our molecular data obtained by cDNA microarray were consistent with the published biochemical and clinical observations of SLHCC and NHCC.CONCLUSION: cDNA microarray is an effective technique in screening the difference in gene expression between SLHCC and NHCC. Many of these differentially expressed genes are involved in the invasion and metastasis of HCC.Further analysis of these genes will help to understand the different molecular mechanisms of SLHCC and NHCC. | Lian-YueYang WeiWang Ji-XiangPeng Jie-QuanYang Gen-WenHuang | 2004 | World Journal of Gastroenterology2004，10，24： | 4 |
| 19 | Activation and involvement of JNK1/2 in hydrogen peroxideinduced neurotoxicity in cultured rat cortical neurons显示文摘AIM: To investigate the role of c-Jun N-terminal protein kinase 1 and 2 (JNK1/2) and the main signal pathway forits activation in hydrogen peroxide (H2O2) induced apoptotic-like cortical cell death. METHODS: Using the modelof oxidative stress induced by H2O2, the expression and diphosphorylation of JNK1/2 was examined by immunoblottinganalysis, and neuronal apoptotic like cell death was determined by 4’,6-diamidino-2-phenylindole (DAPI) staining.RESULTS: The elevation in diphosphorylation level of JNK1/2 (4.40-/5.61-fold vs sham control) was associatedwith the concentration of H2O2 (0-100 mol/L) and the development of apoptotic-like cell death (11.04 %-81.01 %).There was no alteration of JNK1/2 protein expression following H2O2 treatment and recovery at different timepoints. Administration with JNK1/2 antisense oligonucleotides not only significantly decreased JNK1/2 proteinexpression and activation level, but also significantly reduced cortical cell death induced by H2O2 exposure.Furthermore, both JNK1/2 diphosphorylation and apoptotic-like cell death were largely prevented by pretreatmentwith (5S,10R)-()-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine hydrogen maleate (MK-801)or omission of Ca2+ in incubation medium with ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid(EGTA). CONCLUSION: JNK1/2 is activated and participates in H2O2-induced apoptotic-like death in cultured ratcortical neurons mainly via N-methyl-D-aspartate (NMDA) receptor-mediated influx of extracellular Ca2+. | WeiWANG CanGAO Xiao-yuHOU YongLIU Yan-yanZONG Guang-yiZHANG | 2004 | Acta Pharmacologica Sinica2004，25，5： | 3 |
| 20 | Novel KCl/Silicate Drilling Fluids for Alleviating Problems in Troublesome Shale Formations in Sudan显示文摘为几十年,井在块 6 钻了,苏丹,经历了与由于在 Aradeiba 和 Abu Gabra 形成存在的高度反应、散的页岩钻液体有关的严肃的洞不稳定性问题。这些问题包括了洞节与紧密的洞,以及严肃的 sloughing 相结合的冲刷。经常的擦拭器旅行井被要求并且记载不通常是成功的。以前,几常规禁止的以水为基的钻液体象 KCl/polymer, KCl/lime/polymer 和 KCl/PHPA 那样(部分 hydrolyzed polyacrylamide ) 在这个区域被使用了,但是与在洞稳定性和钻的性能的仅仅边缘的改进。最近,一个最新提出的 KCl /sodium 硅酸盐系统,被性质和过滤控制的好 rheological 描绘,被开发并且使用了提供必要禁止的字符。第一试用很好与这种钻被钻在块 6 的液体,苏丹,和下列受益被观察:(1 ) 优秀正直为页岩形成由钻的剪样展出了;(2 ) 稳定的地上凿穿与更低的泥重量保留了;(3 ) 减少了擦拭器旅行;并且(4 ) 从 caliper logs.Later 显示出在上的在里面计量器地上凿穿,五口更多的井在这个区域与 KCl /sodium 硅酸盐系统被钻。KCl/sodium 硅酸盐系统罐头充分遇见在这个区域钻操作的要求,这被发现。案例研究以擦拭器旅行,剪样的禁止的特性,洞条件和泥重量在这份报纸被介绍。 | Guo Jiankang Yan Jienian Fan Weiwang Zhang Hongjing Wang Qunli Yu Zhihai | 2005 | Petroleum Science2005，2，4： | 2 |
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