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| 1 | Development and validation of an HPLC–MS/MS method to determine clopidogrel in human plasma显示文摘A quantitative method for clopidogrel using online-SPE tandem LC–MS/MS was developed and fully validated according to the well-established FDA guidelines.The method achieves adequate sensitivity for pharmacokinetic studies,with lower limit of quantifications(LLOQs)as low as 10 pg/m L.Chromatographic separations were performed on reversed phase columns Kromasil Eternity-2.5-C18-UHPLC for both methods.Positive electrospray ionization in multiple reaction monitoring(MRM)mode was employed for signal detection and a deuterated analogue(clopidogrel-d_4)was used as internal standard(IS).Adjustments in sample preparation,including introduction of an online-SPE system proved to be the most effective method to solve the analyte back-conversion in clinical samples.Pooled clinical samples(two levels)were prepared and successfully used as real-sample quality control(QC)in the validation of back-conversion testing under different conditions.The result showed that the real samples were stable in room temperature for 24 h.Linearity,precision,extraction recovery,matrix effect on spiked QC samples and stability tests on both spiked QCs and real sample QCs stored in different conditions met the acceptance criteria.This online-SPE method was successfully applied to a bioequivalence study of75 mg single dose clopidogrel tablets in 48 healthy male subjects. | Gangyi Liu Chunxia Dong Weiwei Shen Xiaopei Lu Mengqi Zhang Yuzhou Gui Qinyi Zhou Chen Yu | 2016 | Acta Pharmaceutica Sinica B2016，6，1： | 3 |
| 2 | A positive feedback regulatory loop involving the lncRNA PVT1 and HIF-1αin pancreatic cancer显示文摘Extreme hypoxia is among the most prominent pathogenic features of pancreatic cancer(PC).Both the long non-coding RNA(lncRNA)plasmacytoma variant translocation 1(PVT1)and hypoxic inducible factor-1α(HIF-1α)are highly expressed in PC patients and play a crucial role in disease progression.Reciprocal regulation involving PVT1 and HIF-1αin PC,however,is poorly understood.Here,we report that PVT1 binds to the HIF-1αpromoter and activates its transcription.In addition,we found that PVT1 could bind to HIF-1αand increases HIF-1αpost-translationally.Our findings suggest that the PVTl-HIF-1αpositive feedback loop is a potential therapeutic target in the treatment of PC. | Yiping Zhu Fang Wu Weiwei Gui Nan Zhang Erik Matro Linghua Zhu Daniel Turunen Eserberg Xihua Lin | 2021 | Journal of Molecular Cell Biology2021，13，9： | 2 |
| 3 | Eco-friendly biodegradable polyurethane based coating for antibacterial and antifouling performance显示文摘Biofouling, which comprises the absorption of proteins and the adhesion of bacteria to the surface of living entities, is a severe concern for the maritime sector since it ultimately leads to hydrodynamic drag,resulting in a higher increase in fuel consumption. As a result, polymer resins are crucial in the marine sector for anti-biofouling coatings. In this work, the poly(caprolactone-ethylene glycol-caprolactone)-p olyurethane(PECL-PU) are prepared through ε-caprolactone(CL), poly(ethylene glycol)(PEG), 4,4'-methylene bis(cyclohexyl isocyanate) and 1,4 butanediol. Our study demonstrate that the PECL-PU copolymer degraded in artificial seawater(5.21%), enzymatic solution(12.63%), and seawater(13.75%)due to the presence of PEG segments in the laboratory-based test under static condition. Because the addition of PEG segments are increased the polymer's amorphous area and decreased the crystallization of the polycaprolactone(PCL) in the copolymer, as demonstrated by differential scanning calorimetry, X-ray diffraction, and water contact angle studies. Therefore, the hydrolysis rates of PECL-PU were higher than the caprolactone-co-polyurethane(CL-PU). The antifouling test showed that PECL-PU3 copolymer had about 90.29% protein resistance, 85.2% Escherichia coli(E. coli) reduction and 94.61% marine diatom Navicula incerta reduction comparison to the control. We have developed an eco-friendly and inexpensive promising degradable polyurethane for reduction of bacterial biofilm, which can preserve the formation of biofouling on marine coating under practical sea conditions. | Abid Ali Bilal Ul Amin Wenwu Yu Taijiang Gui Weiwei Cong Kai Zhang Zheming Tong Jiankun Hu Xiaoli Zhan Qinghua Zhang | 2023 | Chinese Journal of Chemical Engineering2023，54，2： | 1 |
| 4 | Knockdown of insulin-like growth factor 2 gene disrupts mitochondrial functions in the liver显示文摘Even though insulin-like growth factor 2(IGF2)has been reported to be overexpressed in nonalcoholic fatty liver disease(NAFLD),its role in the progression of NAFLD and the potential mechanism remain largely unclear.Using in vitro models,we found that IGF2 was the key overexpressed gene in steatosis,suggesting a possible association between IGF2 and NAFLD.Interestingly,loss-of-function experiments revealed that inhibition of IGF2 protein impaired mitochondrial biogenesis and respiration.It additionally disrupted the expression changes of mitochondrial fusion and fission-related proteins necessary in maintaining mitochondrial homeostasis.Consistently,IGF2 knockdown reduced the mitochondrial membrane potential and increased the production of reactive oxygen species.Mechanistically,IGF2 regulates mitochondrial functions by modulating the expression of SIRT1 and its downstream gene PGCla.This research opens a new frontier on the role of IGF2 in energy metabolism,which potentially participates in the development of NAFLD.As such,IGF2 is a potential therapeutic target against NAFLD. | Weiwei Gui Yiyi Zhu Shuiya Sun Weifen Zhu Bowen Tan Hanxin Zhao Chengxin Shang Fenping Zheng Xihua Lin Hong Li | 2021 | Journal of Molecular Cell Biology2021，13，8： | 1 |
| 5 | Circumventing chemo-mechanical failure of Sn foil battery anode by grain refinement and elaborate porosity design显示文摘Tin (Sn) metal foil is a promising anode for next-generation high-energy–density lithium-ion batteries (LIBs) due to its high capacity and easy processibility. However, the pristine Sn foil anode suffers nonuniform alloying/dealloying reaction with lithium (Li) and huge volume variation, leading to electrode pulverization and inferior electrochemical performance. Herein, we proposed that reduced grain size and elaborate porosity design of Sn foil can circumvent the nonuniform alloy reaction and buffer the volume change during the lithiation/delithiation cycling. Experimentally, we designed a three-dimensional interconnected porous Sn (3DIP-Sn) foil by a facile chemical alloying/dealloying approach, which showed improved electrochemical performance. The enhanced structure stability of the as-fabricated 3DIP-Sn foil was verified by chemo-mechanical simulations and experimental investigation. As expected, the 3DIP-Sn foil anode revealed a long cycle lifespan of 4400 h at 0.5 mA cm^(−2) and 1 mAh cm^(−2) in Sn||Li half cells. A 3DIP-Sn||LiFePO_(4) full cell with LiFePO_(4) loading of 7.1 mg cm^(−2) exhibited stable cycling for 500 cycles with 80% capacity retention at 70 mA g^(−1). Pairing with high-loading commercial LiNi0.6Co0.2Mn0.2O_(2) (NCM622, 18.4 mg cm^(−2)) cathode, a 3DIP-Sn||NCM622 full cell delivered a high reversible capacity of 3.2 mAh cm^(−2). These results demonstrated the important role of regulating the uniform alloying/dealloying reaction and circumventing the localized strain/stress in improving the electrochemical performance of Sn foil anodes for advanced LIBs. | Shuibin Tu Xin Ai Xiancheng Wang Siwei Gui Zhao Cai Renming Zhan Yuchen Tan Weiwei Liu Hui Yang Chenhui Li Yongming Sun | 2021 | Journal of Energy Chemistry2021，30，11： | 0 |
| 6 | Regulation mechanism and pathogenic role of lncRNA plasmacytoma variant translocation 1 (PVT1) in human diseases显示文摘Plasmacytoma variant translocation 1 (PVT1) is a long non-coding RNA (lncRNA) gene identified as a recurrent breakpoint of Burkitt’s lymphomas. Human PVT1 gene is located on region 8q24.21, a well-known cancer risk region, and encodes at least 26 linear ncRNA isoforms and 26 circular RNA isoforms, as well as 6 microRNAs. Several PVT1 functioning models have been reported recently such as competing endogenous RNA (ceRNA) activity and regulating protein stability of oncogenes, especially MYC oncogene. The promoter of PVT1 gene is a boundary element of tumor-suppressor DNA. CircPVT1 derived from PVT1 gene is also a critical non-coding oncogenic RNA. Although substantial advancements have been made in understanding the roles of PVT1 in cancer recently, the detailed mechanisms underlying its functions remain unclear. Herein, we summarize the recent progressions on the mechanisms underlying PVT1 regulated gene expression at different levels. We also discuss the interaction between lncRNA and protein, RNA and DNA, as well as the potential cancer therapy strategy by targeting these networks. | Fang Wu Yiping Zhu Caiping Zhou Weiwei Gui Hong Li Xihua Lin | 2023 | Genes & Diseases2023，10，3： | 0 |
| 7 | Characterization of a Thermophilic Monosaccharide Stimulated β-Giucosidase from Acidothermus cellulolyticus显示文摘 | LI Yuwei BU Mingwei CHEN Peng LI Xiaohong CHEN Changwu GAO Gui FENG Yan HAN Weiwei ZHANG Zuoming | 2018 | Chemical Research in Chinese Universities2018，34，2： | 0 |
| 8 | Severe cases of BCGosis-susceptible primary immunodeficiency diseases identified by next-generation sequencing:Implications for adjustment of BCG vaccination timing in China显示文摘The World Health Organization(WHO)recommends neonatal Bacillus Calmette-Guerin(BCG)vaccination in countries with a high prevalence of tuberculosis(TB),which has been practiced in more than 150 countries(WHO,2004).The prevalence of TB is a serious condition in China,ranking as the second in the high epidemic countries globally(WHO,2018).China initiated a neonatal BCG vaccination program in 1986 and reached an average vaccination rate of 99%in 2016 with a great success in preventing the incidence of TB(WHO,2018). | Gang Liu Haijuan Xiao Linlin Liu Lingyun Guo Ruolan Guo Xuyun Hu Chanjuan Hao Jingang Gui Weiwei Jiao Fang Xu Adong Shen Wei Li | 2020 | Journal of Genetics and Genomics2020，47，4： | 0 |
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