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| 1 | Auxin response factor 6A regulates photosynthesis,sugar accumulation,and fruit development in tomato显示文摘Auxin response factors(ARFs)are involved in auxin-mediated transcriptional regulation in plants.In this study,we performed functional characterization of SlARF6A in tomato.SlARF6A is located in the nucleus and exhibits transcriptional activator activity.Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants,whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type(WT)plants.Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants.Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars,whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits.RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism,photosynthesis and sugar metabolism.SlARF6A directly bound to the promoters of SlGLK1,CAB,and RbcS genes and positively regulated the expression of these genes.Overexpression of SlARF6A also inhibited fruit ripening and ethylene production,whereas downregulation of SlARF6A increased fruit ripening and ethylene production.SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression.Taken together,these results expand our understanding of ARFs with regard to photosynthesis,sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops. | Yujin Yuan Xin Xu Zehao Gong Yuwei Tang Mengbo Wu Fang Yan Xiaolan Zhang Qian Zhang Fengqing Yang Xiaowei Hu Qichen Yang Yingqing Luo Lihua Mei Wenfa Zhang Cai-Zhong Jiang Wangjin Lu Zhengguo Li Wei Deng | 2019 | Horticulture Research2019，6，1： | 3 |
| 2 | The impact of concurrent variation of atmospheric meridional heat transport in western Baffen Bay and eastern Greenland on summer Arctic sea ice显示文摘Based on the climatological reanalysis data of the European Center for Medium-Range Weather Forecasts and the Arctic sea ice data of the National Snow and Ice Data Center, the relationship between the Arctic sea ice area(SIA)and the interannual variation of atmospheric meridional heat transport(AMHT) was analyzed. The results show that the atmospheric meridional heat transported by transient eddy(TAMHT) dominates the June AMHT in midhigh latitudes of the Northern Hemisphere, while the western Baffin Bay(B) and the eastern Greenland(G) are two gates for TAMHT entering the Arctic. TAMHT in the western Baffin Bay(B-TAMHT) and eastern Greenland(G-TAMHT) has a concurrent variation of reverse phase, which is closely related to the summer Arctic SIA.Possible mechanism is that the three Arctic atmospheric circulation patterns(AD, AO and NAO) in June can cause the concurrent variation of TAMHT in the B and G regions. This concurrent variation helps to maintain AD anomaly in summer through wave action and changes the polar air temperature, thus affecting the summer Arctic SIA. Calling the heat entering the Arctic as warm transport and the heat leaving Arctic as cold transport, then the results are classified into three situations based on B-TAMHT and G-TAMHT: warm B corresponding to cold G(WC), cold B corresponding to warm G(CW), cold B corresponding to cold G(CC), while warm B corresponding to warm G is virtually non-existent. During the WC situation, the SIA in the Pacific Arctic sediments and Kara Sea decreases;during the CW situation, the SIA in the Laptev Sea and Kara Sea decreases;during the CC situation, the SIA in the Kara Sea, Laptev Sea and southern Beaufort Sea increases. | Le Wang Lujun Zhang Wenfa Yang | 2020 | Acta Oceanologica Sinica2020，39，8： | 1 |
| 3 | A high energy nanosecond cryogenic cooled Yb:YAG active-mirror amplifier system显示文摘A diode-pumped master oscillator power amplifier system based on a cryogenic Yb:YAG active-mirror laser has been developed.The performances of the laser amplifier at low temperature and room temperature have been investigated theoretically and experimentally.A maximum output energy of 3.05 J with an optical-to-optical efficiency of 14.7% has been achieved by using the master amplifier system. | Xiaojin Cheng Jianlei Wang Zhongguo Yang Jin Liu Lei Li Xiangchun Shi Wenfa Huang Jiangfeng Wang Weibiao Chen | 2014 | High Power Laser Science and Engineering2014，2，3： | 1 |
| 4 | Ultra-high general and special color rendering index white organic light-emitting device based on a deep red phosphorescent dye显示文摘 | Yang Li Wei Zhang Letian Zhang Xuemei Wen Yongming Yin Shihao Liu Wenfa Xie Hongyu Zhao Silu Tao | 2013 | Organic Electronics2013，，12： | 1 |
| 5 | High efficiency small molecule white organic light-emitting devices with a multilayer structure显示文摘 | Huishan Yang Wenfa Xie Yi Zhao Jingying Hou Shiyong Liu | 2006 | Solid State Communications2006，，9： | 1 |
| 6 | Attenuation of Telomerase Activity by siRNA Targeted Telomerase RNA Leads to Apoptosis and Inhibition of Proliferation in Human Renal Carcinoma Cells显示文摘OBJECTIVE Telomerase is an attractive molecular target for cancer therapy because the activation of telomerase is one of the key steps in cell immortalization and carcinogenesis. RNA interference using small-interfering RNA (siRNA) has been demonstrated to be an effective method for inhibiting the expression of a given gene in human cells. The aim of the present study was to investigate whether inhibition of telomerase activity by siRNA targeted against human telomerase RNA (hTR) can inhibit proliferation and induce apoptotic cell death in human renal carcinoma cells (HRCCs). METHODS The siRNA duplexes for hTR were synthesized and 786-0 HRCCs were transfected with different concentrations of hTR-siRNA. The influence on the hTR mRNA level, telomerase activity, as well as the effect on cell proliferation and apoptosis was examined. RESULTS Anti-hTR siRNA treatment of HRCCs resulted in specific reduction of hTR mRNA and inhibition of telomerase activity. Additionally, significant inhibition of proliferation and induction of apoptosis were observed. CONCLUSION siRNA against the hTR gene can inhibit proliferation and induce apoptosis by blocking telomerase activity of HRCCs. Specific hTR inhibition by siRNA represents a promising new option for renal cancer treatment. | Rumin Wen Junjie Liu Wang Li Wenfa Yang Lijun Mao Junnian Zheng | 2006 | Chinese Journal of Clinical Oncology2006，3，5： | 0 |
| 7 | A critical review on thermodynamic mechanisms of membrane fouling in membrane-based water treatment process显示文摘Membrane technology is widely regarded as one of the most promising technologies for wastewater treatment and reclamation in the 21st century. However, membrane fouling significantly limits its applicability and productivity. In recent decades, research on the membrane fouling has been one of the hottest spots in the field of membrane technology. In particular, recent advances in thermodynamics have substantially widened people’s perspectives on the intrinsic mechanisms of membrane fouling. Formulation of fouling mitigation strategies and fabrication of anti-fouling membranes have both benefited substantially from those studies. In the present review, a summary of the recent results on the thermodynamic mechanisms associated with the critical adhesion and filtration processes during membrane fouling is provided. Firstly, the importance of thermodynamics in membrane fouling is comprehensively assessed. Secondly, the quantitative methods and general factors involved in thermodynamic fouling mechanisms are critically reviewed. Based on the aforementioned information, a brief discussion is presented on the potential applications of thermodynamic fouling mechanisms for membrane fouling control. Finally, prospects for further research on thermodynamic mechanisms underlying membrane fouling are presented. Overall, the present review offers comprehensive and in-depth information on the thermodynamic mechanisms associated with complex fouling behaviors, which will further facilitate research and development in membrane technology. | Jiaheng Teng Ying Deng Xiaoni Zhou Wenfa Yang Zhengyi Huang Hanmin Zhang Meijia Zhang Hongjun Lin | 2023 | Frontiers of Environmental Science & Engineering2023，17，10： | 0 |
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