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| 1 | Loss of YTHDF2-mediated m6A-dependent mRNA clearance facilitates hematopoietic stem cell regeneration显示文摘 | Hu Wang Hongna Zuo Jin Liu Fei Wen Yawei Gao Xudong Zhu Bo Liu Feng Xiao Wengong Wang Gang Huang Bin Shen Zhenyu JU | 2018 | Cell Research2018，28，10： | 16 |
| 2 | Purification and Characterization of a New Thermostable κ-CarrageenasefromtheMarineBacterium Pseudoalteromonas sp. QY203显示文摘A new extracellular κ-carrageenase, namely CgkP, 34.0 kDa in molecular weight, was purified from Pseudoalteromonas sp. QY203. CgkP showed relatively high activity at acidities ranging from pH6.0 to pH9.0 and temperatures ranging from 30℃ to 50℃ with the highest activity at 45℃ and pH7.2. Sodium chloride increased its activity markedly, and KCl increased its activity slightly. The divalent and trivalent metal ions including Cu2+ , Ni2+ , Zn2+ , Mn2+ , Al3+ and Fe3+ significantly inhibited its activity, while Mg2+ did not. CgkP remained 70% of original activity after being incubated at 40℃ for 48 h, and remained 80% of the activity after being incubated at 45℃ for 1 h. It exhibited endo-κ-carrageenase activity, mainly depolymerizing the κ-carrageenan into disaccharide and tetrasaccharide. CgkP was more thermostable than most of previously reported κ-carrageenases with a potential of being used in industry. | LI Shangyong JIA Panpan WANG Linna YU Wengong HAN Feng | 2013 | Journal of Ocean University of China2013，12，1： | 10 |
| 3 | Preparation and characterization of a novel strong-fluorescent hydrotalcite-like compound (Al-HTLc)显示文摘A novel strong-fluorescent hydrotalcite-like compound (Al-HTLc) was synthesized by coprecipitation. In the sample, the content of aluminum(III) in the layers was decreased to a proper value. The Al3+ ions coordinated with 8-hydroxyquinolines (8-HQ) which were dispersed into the anions in the interlayer region. The sample was characterized by XRD, XPS, FT-IR, TG-DSC, UV-Vis, and fluorescence spec- troscopy; its composition and structure were also determined. The results indicate that the sample can emit fluorescence (487 nm) with a strong fluorescence intensity (4.9×105 (a.u.)). The fluorescent lifetime and fluorescence quantum yield of Al-HTLc were measured to be 21.24 ns and 67%, respectively, higher than those of pure 8-hydroxyquinoline aluminum (Alq3). The result of TG-DSC measurement clearly shows the enhanced thermal stability of Al-HTLc compared with that of MgAl-LDH and pure Alq3. Al-HTLc may be used as a novel luminescent functional material. | ZHANG WenGong CHEN Hong | 2008 | Science China Chemistry2008，51，9： | 6 |
| 4 | Cloning and Characterization of a New κ-Carrageenase Gene from Marine Bacterium Pseudoalteromonas sp. QY203显示文摘κ-carrageenan oligosaccharides exhibit various biological activities. Enzymatic degradation by κ-carrageenase is safe and controllable. Therefore, κ-carrageenases have captured more and more attentions. In this study, a κ-carrageenase encoding gene, cgk X, was cloned from Pseudoalteromonas sp. QY203 with degenerate and inverse PCR. It comprised an ORF of 1194 bp in length, encoding a protein with 397 amino acid residues. Cgk X is a new member of glycoside hydrolase family 16. The deduced amino acid sequence shared a high similarity with Cgk X of Pseudoalteromonas κ-carrageenase; however, the recombinant Cgk X showed different biochemical characteristics. The recombinant enzyme was most active at p H 7.0 and 55℃ in the presence of 300 mmol L^(^(-1))Na Cl. It was stable in a broad range of acidity ranging from p H 3.0 to p H 10.0 when temperature was below 40℃. More than 80% of its activity was maintained after being incubated at p H 3.6–10.0 and 4℃ for 24 h. Cgk X retained more than 90% of activity after being incubated at 40℃ for 1 h. EDTA and SDS(1 mmol L^(-1)) did not inhibit its activity. Cgk X hydrolyzed κ-carrageenan into disaccharide and tetrasaccharide as an endo-cleaver. All these characteristics demonstrated that Cgk X is applicable to both κ-carrageenan oligosaccharide production and κ-carrageenase structure-function research. | XU Xiaoyan LI Shangyong YANG Xuemei YU Wengong HAN Feng | 2015 | Journal of Ocean University of China2015，14，6： | 5 |
| 5 | Beamlet prestack depth migration and illumination: A test based on the Marmousi model显示文摘Beamlet 来源容易让强壮的本地、方向性的人物和罐头完成本地照明和移植。而且,他们比常规迁居方法提供更好的迁居结果。我们介绍让的横梁的基本原则包括窗口的 Fourier 变换和框架理论的 prestack 深度移植。我们基于 Gaussian 功能解释 Gabor-Daubechies (G-D ) 框架。Beamlet 分解在波浪地的本地空格和方向提供信息。我们综合横梁让来源和横梁在使用矩形和 Gaussian 窗口的小浪域让记录然后外推有一个 Fourier 有限差别的操作符的综合数据。我们用标准 Marmousi 模型测试方法。由比较并且分析让并且微笑的方向性的横梁与不同窗户和方向让的单身者的迁居结果,我们证明有 Gaussian 横梁的 prestack 深度迁居的有效性让方法。 | Ye Yueming Li Zhenchun Han Wengong Liu Qingmin | 2006 | Applied Geophysics2006，3，4： | 5 |
| 6 | Synthesis and characterization of a strong-fluorescent Eu-containing hydrotalcite-like compound显示文摘A novel strong-fluorescent Eu-containing hydrotalcite-like compound (Eu-HTlc) was synthesized using the coprecipitation method,in which aluminum(III) was partially substituted by Europium (III) in the hydrotalcite-like layers,and thenoyltrifluoroacetone,1,10-phenanthroline were dispersed into the anions in the interlayer region.The sample was characterized by XRD,XPS,FT-IR,ICP,TG-DSC,TEM and fluorescence spectra,and its composition and structure were determined.The results indicated that the sample exhibited a characteristic red light (614 nm).The fluorescent lifetime and fluorescence quantum yield of Eu-HTlc were measured to be respectively 893 -s and 66.44%,higher than those of Eu(III)-thenoyltrifluoroacetone1,10-phenanthroline complex [Eu(TTA)3phen].The result of TG-DSC measurement showed the enhanced thermal stability of Eu-HTlc compared with that of MgAl-LDHs and Eu(TTA)3phen.With excellent photoluminescent property and thermal stability,low contents of rare earth ions and ligands,the Eu-HTlc may become one of the novel fluorescent materials with potential applications. | CHEN Hong & ZHANG WenGong Institute of Polymer Science,College of Chemistry and Materials Science,Fujian Normal University,Fuzhou 350007,China | 2010 | Science China Chemistry2010，53，6： | 5 |
| 7 | 原发性肺血管肉瘤1例报道(英文)显示文摘A 40-year-old man with haemoptysis and serious spontaneous hemothorax. Chest computed tomography showed multiple flakes in his lungs, nodules shadow, right pleural effusion with right pulmonary atelectasis. The pathological specimens obtained by thoracic surgery revealed pulmonary angiosarcoma. Primary pulmonary angiosarcoma is a rare malignant tumor and is lack of typical clinical symptoms and imaging features, diagnosis mainly depend on histopathologic features and immunohistochemical analysis. There is no effective therapy for pulmonary vascular sarcoma. Integrated treatment can may extend the survival time of patients. | Yong Zhou Wengong Wang Qinghai Zhang | 2012 | The Chinese-German Journal of Clinical Oncology2012，11，10： | 2 |
| 8 | Cartilage oligomeric matrix protein is an endogenous β-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury显示文摘Compelling evidence has revealed that biased activation of G protein-coupled receptor(GPCR)signaling,including angiotensin II(AngII)receptor type 1(AT1)signaling,plays pivotal roles in vascular homeostasis and injury,but whether a clinically relevant endogenous biased antagonism of AT1 signaling exists under physiological and pathophysiological conditions has not been clearly elucidated.Here,we show that an extracellular matrix protein,cartilage oligomeric matrix protein(COMP),acts as an endogenous allosteric biased modulator of the AT1 receptor and its deficiency is clinically associated with abdominal aortic aneurysm(AAA)development.COMP directly interacts with the extracellular N-terminus of the AT1 via its EGF domain and inhibits AT1-β-arrestin-2 signaling,but not Gq or Gi signaling,in a selective manner through allosteric regulation of AT1 intracellular conformational states.COMP deficiency results in activation of AT1a-β-arrestin-2 signaling and subsequent exclusive AAA formation in response to AngII infusion.AAAs in COMP–/–or ApoE–/–mice are rescued by AT1a orβ-arrestin-2 deficiency,or the application of a peptidomimetic mimicking the AT1-binding motif of COMP.Explorations of the endogenous biased antagonism of AT1 receptor or other GPCRs may reveal novel therapeutic strategies for cardiovascular diseases. | Yi Fu Yaqian Huang Zhao Yang Yufei Chen Jingang Zheng Chenfeng Mao Zhiqing Li Zhixin Liu Bing Yu Tuoyi Li Meili Wang Chanjuan Xu Yiwei Zhou Guizhen Zhao Yiting Jia Wei Guo Xin Jia Tao Zhang Li Li Ziyi Liu Shengchao Guo Mingliang Ma Heng Zhang Bo Liu Junbao Du Wengong Wang Chaoshu Tang Pei Gao Qingbo Xu Xian Wang Jianfeng Liu Jinpeng Sun Wei Kong | 2021 | Cell Research2021，31，7： | 2 |
| 9 | Research on successive preparation of nano-FeNi alloy and its ethanol sol by pulsed laser ablation显示文摘Nano-FeNi alloy/ethanol sol and its nano-FeNi alloy have been successively pre-pared by pulsed laser ablation at the interface of flowing ethanol and rotative FeNi alloy target. The energy spectrum analysis shows that the weight ratio of constituents of the Fe and Ni in the nano-FeNi alloy particles is about 66︰34. The TEM shows that the nano-FeNi alloy particles original sizes are about 1—5 nm, and the particle sizes gradually become 10—50 nm due to self-congregation during 1—3 d. The XRD analysis indicates that the nano-FeNi alloy particles are almost in non-crystalline. It is found that there are one strong excitation peak at 311.00 nm and two weak excitation peaks at 273.00 and 347.00 nm, and there is one strong emission peak at 418.00 nm when it is excitated by 311.00 nm at room temperature from the fluorescence spectrum of nano-FeNi alloy/ethanol sol. The UV-Vis spectrum of nano-FeNi alloy/ethanol sol indicates that there exist one strong absorption peak which is splitted into 9 distinguished small peaks at about 230.00 nm and one weak exciton absorption peak at 275.00 nm. The FT-IR spectrum of nano-FeNi alloy/ethanol sol exhibits that the stretching vibration absorption peak of associating hydroxyl (nO—H) in the sol has the distinct blue shift, and confirms that the surface of nano-FeNi alloy particles has been modified by carbon-hydrogen organic group from ethanol decomposition in the producing process of the nano-FeNi alloy/ethanol sol. | ZHANG Wengong JIN Zhaoguo | 2004 | Science China Chemistry2004，47，2： | 2 |
| 10 | A New κ-Carrageenase Cgk S from Marine Bacterium Shewanella sp. Kz7显示文摘A new κ-carrageenase gene cgk S was cloned from marine bacterium Shewanella sp. Kz7 by using degenerate and site-finding PCR. The gene was comprised of an open reading frame of 1224 bp, encoding 407 amino acid residues, with a signal peptide of 24 residues. Based on the deduced amino acid sequence, the κ-carrageenase Cgk S was classified into the Glycoside Hydrolase family 16. The cgk S gene was expressed in Escherichia coli, and the recombinant enzyme was purified to homogeneity with a specific activity of 716.8 U mg-1 and a yield of 69%. Recombinant Cgk S was most active at 45℃ and p H 8.0. It was stable at p H 6.0–9.0 and below 30℃. The enzyme did not require Na Cl for activity, although its activity was enhanced by Na Cl. Cgk S degraded κ-carrageenan in an endo-fashion releasing tetrasaccharides and disaccharides as main hydrolysis products. | WANG Linna LI Shangyong ZHANG Shilong LI Jiejing YU Wengong GONG Qianhong | 2015 | Journal of Ocean University of China2015，14，4： | 2 |
| 11 | Enhancing the Thermostability of a Novel β-agarase AgaB through Directed Evolution显示文摘 | Chao Shi Xinzhi Lu Cuiping Ma Yiming Ma Xiaoyan Fu Wengong Yu | 2008 | Applied Biochemistry and Biotechnology2008，，1： | 1 |
| 12 | Safety of Bevacizumab in Treating Metastatic Colorectal Cancer: A Systematic Review and Meta-analysis of All Randomized Clinical Trials显示文摘 | Fei Dai Lixing Shu Yangfang Bian Zhuo Wang Zhangwei Yang Wengong Chu Shen Gao | 2013 | Clinical Drug Investigation2013，，11： | 1 |
| 13 | Purification and Refolding of a Novel β-Agarase from Inclusion Body of E. coli显示文摘β-agarase AgaB appears to represent a new family of glycoside hydrolase; it is structurally and functionally different from other known agarases. In the present study, AgaB was expressed with a temperature-inducible expression system in E. coli BL21 (DE3) as a fusion protein bearing a C-terminal hexahistidine tag. The protein existed mainly in the form of inclusion body. After being washed and solubilized, AgaB in inclusion body was denatured and purified to electrophoretic purity by immobilized metal affinity chromatography. The purified AgaB was then refolded using a simple pulse dilution method, and the refolded AgaB showed a high specific hydrolysis activity of about 1600 units /mg protein. Forty milligrams of refolded pure protein were obtained from 1L of culture. | ZHANG Li, LU Xinzhi, HAN Feng, MA Cuiping, and YU Wengong* Marine Drug and Food Institute, Ocean University of China, Qingdao 266003, P.R. China | 2007 | Journal of Ocean University of China2007，6，1： | 1 |
| 14 | Increased AMP:ATP Ratio and AMP-activated Protein Kinase Activity during Cellular Senescence Linked to Reduced HuR Function显示文摘 | Wengong Wang Xiaoling Yang | 2003 | The Journal of Biological Chemistry2003，278，27： | 1 |
| 15 | Increasing the transient expression of GUS gene in Porphyra yezoensis by 18S rDNA targeted homologous recombination显示文摘 | Hongquan Liu Wengong Yu Jixun Dai Qianhong Gong Kunfeng Yang Yaping Zhang | 2003 | Journal of Applied Phycology2003，，5： | 1 |
| 16 | Safety of Bevacizumab in Treating Metastatic Colorectal Cancer: A Systematic Review and Meta-analysis of All Randomized Clinical Trials显示文摘 | Fei Dai Lixing Shu Yangfang Bian Zhuo Wang Zhangwei Yang Wengong Chu Shen Gao | 2013 | Clinical Drug Investigation2013，，11： | 1 |
| 17 | The hydrophobic cluster on the surface of protein is the key structural basis for the SDS-resistance of chondroitinase VhChlABC显示文摘The application of chondroitinase requires consideration of the complex microenvironment of the target.Our previous research reported a marine-derived sodium dodecyl sulfate(SDS)-resistant chondroitinase VhChlABC.This study further investigated the mechanism of VhChlABC resistance to SDS.Focusing on the hydrophobic cluster on its strong hydrophilic surface,it was found that the reduction of hydrophobicity of surface residues Ala181,Met182,Met183,Ala184,Val185,and Ile305 significantly reduced the SDS resistance and stability.Molecular dynamics(MD)simulation and molecular docking analysis showed that I305G had more conformational flexibility around residue 305 than wild type(WT),which was more conducive to SDS insertion and binding.The affinity of A181G,M182A,M183A,V185A and I305G to SDS was significantly higher than that of WT.In conclusion,the surface hydrophobic microenvironment composed of six residues was the structural basis for SDS resistance.This feature could prevent the binding of SDS and the destruction of hydrophobic packaging by increasing the rigid conformation of protein and reducing the binding force of SDS-protein.The study provides a new idea for the rational design of SDS-resistant proteins and may further promote chondroitinase research in the targeted therapy of lung diseases under the pressure of pulmonary surfactant. | Juanjuan Su Hao Wu Chengying Yin Fengchao Zhang Feng Han Wengong Yu | 2024 | Marine Life Science & Technology2024，6，1： | 0 |
| 18 | HuR and post-transcriptional regulation in vascular aging显示文摘HuR(ELAV11(embryonic lethal,abnormal vision)-like 1),a ubiquitously expressed member of the ELAV-like RNA-binding protein family,has been shown to regulate the stability and translation of mRNAs that encode factors regulating cellular senescence,thereby impacting on aging.In this review,we discuss the current knowledge of HuR’s role in vascular cell senescence and vascular aging. | WANG WenGong | 2014 | Science China(Life Sciences)2014，57，8： | 0 |
| 19 | Prevention of Reservoir Water Bloom by Bio-manipulation显示文摘Siminghu Reservoir already transformed into ' the algae muddy' type,and had nonclassical bio-manipulation practice using Hypophthalmichthys molitix and Aristichthys nobilis to feed the plankton,control water bloom and transform the organic matters into fish product. Through the analysis of the limited data,we think that water bloom of Siminghu Reservoir can be prevented to some extent by breeding H. molitix and A. nobilis,and it can slow down eutrophication of reservoir but cannot control the eutrophication tendency. We must take a variety of comprehensive management measures to control eutrophication from the angle of basin control,and guarantee safety of drinking water resources. | Yu Wengong | 2015 | Meteorological and Environmental Research2015，6，2： | 0 |
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