2篇
您的检索式：作者名="WenjunLiao"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The Inhibitory Effects of Mouse ICOS-Ig Gene-Modified Mouse Dendritic Cells on T Cells显示文摘The main approach to reduce graft rejection has been focused on the development of immunosuppressive agents at present. Although these strategies have reportedly reduced graft rejection, there has been a reciprocal increase in more severe immunosuppression and lethal infections, as well as severe side effects. Blockade of costimulatory T cell response has been proved as one of useful strategies to reduce graft rejection. Furthermore,it has been shown that infusion of dendritic cells (DCs) with a potent negative regulatory ability for T cells could prolong allograft survival. In this study mouse DCs (mDCs) were transfected with the recombinant plasmid pcDNA3.0 containing mouse inducible costimulator-Ig (mICOS-Ig) cDNA by electroporation. The transient expression of mICOS-Ig in mDC could be detected by ELISA and SDS-PAGE. Mouse ICOS-Ig fusion protein expressed in mDC and mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in mixed lymphocyte culture (MLC) in vitro. Furthermore, mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in recipient mice. These results suggested that mICOS-Ig gene-modified mDC exerted inhibitory effects on T cells, and might be suitable for treatment or prevention of graft rejection and immunopathologicdiseases. | GuohuaWang LijuanZhu PingHu HuifenZhu PingLei WenjunLiao BingYu FeiliGong GuanxinShen | 2004 | Cellular & Molecular Immunology2004，1，2： | 1 |
| 2 | FADDdel-GFP-Modified Mouse Insulinoma Cells Counteract the Cytotoxicity of Reactive T Cells显示文摘IDDM results from pancreatic beta cell destruction by islet-reactive T cells, a process that involves beta cell apoptosis. FasL-Fas pathway plays a major role in pancreatic beta cell death. Fas-associated death domain protein (FADD), the component of the tumor necrosis factor receptor type 1 (TNF-R1) and Fas signaling complexes, is involved in TNF-R1- and Fas-induced apoptosis. Inhibiting the function of FADD will lead to blocking downstream apoptosis signal, which protects pancreatic beta cells from destruction by FasL-Fas pathway. In this study we constructed eukaryotic expressing vector of fusional protein FADDdel-GFP named pFADDdeI-GFP. After pFADDdel-GFP was transfected into NIT, the expression of FADDdel-GFP in NIT was detected by fluorescence microscopy and the resistance of NIT transfected with pFADDdel-GFP to cytotoxicity mediated by special T cells was detected by FACS and MTT. The results showed that NIT modified by pFADDdel-GFP obviously resisted cytotoxicity mediated by special T cells. Therefore, it may be useful in the prevention or treatment of IDDM by intervening FasL-Fas pathway. Cellular & Molecular Immunology. 2004;1(5):383-386. | PingHu GuohuaWang XiaohuaZhu JingYang HuifenZhu ZihuiXu WenjunLiao XiaoLiu FenXu JiaoYin GuanxinShen | 2004 | Cellular & Molecular Immunology2004，1，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员