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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | TT viral infection through blood transfusion:retrospective investigation on patients in a prospective study of post-transfusion hepatitis显示文摘AIM To investigate the role of bloodtransfusion in TT viral infection(TTV).METHODS We retrospectively studied serumsamples from 192 transfusion recipients whounderwent cardiovascular surgery and bloodtransfusion between July 1991 and June 1992.Allpatients had a follow-up every other week for atleast 6 months after transfusion.Eightyrecipients received blood before screeningdonors for hepatitis C antibody(anti-HCV),and112 recipients received screened blood.Recipients with alanine aminotransferase level>2.5 times the upper normal limit were testedfor serological markers for viral hepatitis A,B,C,G,Epstein-Barr virus and cytomegalovirus.TTV infection was defined by the positivity forserum TTV DNA using the polymerase chainreaction method.RESULTS Eleven and three patients,whoreceived anti-HCV unscreened and screened'blood,respectively,had serum ALT levels>90 IU/L.Five patients(HCV and TTV:1;HCV,HGV,and TTV:1;TTV:2;and CMV and TTV:1)were positive for TTV DNA,and four of them hadsero-conversion of TTV DNA.CONCLUSION TTV can be transmitted viablood transfusion.Two recipients infected byTTV alone may be associated with the hepatitis.However,whether TTV was the causal agentremains unsettled,and further studies arenecessary to define the role of TTV infection inchronic hepatitis. | Yang SS Wu CH Chen TH Huang YY Huang CS | 2000 | World Journal of Gastroenterology2000，6，1： | 8 |
| 3 | Species distribution of polymeric aluminium ferrum——timed complexation colorimetric analysis method of Al-Fe-Ferron显示文摘The effects of the colorimetric buffer solutions were investigated while the two colorimetric reactions of A1 ferron complex and Fe ferron complex occurred individually, and the effects of the testing wavelength and the pH of the solutions were also investigated. A timed complexation colorimetric analysis method of A1 Fe ferron in view of the total concentration of {A1+Fe} was then established to determine the species distribution of polymeric Al Fe. The testing wavelength was recommended at 362 nm and the testing pH value was 5. With a comparison of the ratios of n Al / n Fe , the standard adsorption curves of the polymeric Al Fe solutions were derived from the experimental results. Furthermore, the solutions’composition were various in both the molar n Al / n Fe ratios, i.e. 0/0, 5/5, 9/1 and 0/10, and the concentrations associated with the total [A1+Fe] which ranged from 10 -5 to 10 -4 mol/L. | Hu, YY Tu, CQ Wu, HH | 2001 | Journal of Environmental Sciences2001，13，4： | 8 |
| 4 | P53 codon 72 polymorphism contributes to breast cancer risk: a metaanalysis based on 39 case-control studies(摘要)显示文摘 | Zhang, ZZ Wang, ML Wu, DM Wang, MM Tong, N Tian, YY Zhang, ZD | 2010 | 南京医科大学学报(自然科学版)2010，30，7： | 3 |
| 5 | Association between social support and health outcomes:a meta-analysis 显示文摘 | Wang HH Wu SZ Liu YY | 2003 | Kaohsiung J Med Sci2003，19，7： | 2 |
| 6 | A new phytoecdysteroid from Ajuga taiwanensis显示文摘 | Chan YY Wu TS Kuoh CS | 2005 | Chem Pharm Bull2005，53，7： | 1 |
| 7 | Four orchid ( Oncidium Cower Ramsey) API/AGL9-1ike MADS box genes show novel expression patterns and cause different effects on floral transi- tion and formation in Arabidopsis thaliana显示文摘 | Chang YY Chiu YF Wu JW | 2009 | Plant and Cell Physiology2009，50，8： | 1 |
| 8 | Functional Studies of lymphocytesusing RNAi technology显示文摘 | Lin YY Hung CF Wu TC | 2006 | Transfusion Medicine and Hemotherapy2006，33，1： | 1 |
| 9 | Lysophosphatidic acid activates L-arginine/ nitric oxide pathway of platelets in rats显示文摘 | Cui YY Zhang LK Wu QZ | 2005 | J Journal of peking university (Health sciences)2005，376，： | 1 |
| 10 | Relationship between endogenous concentrations and serum cholesteryl ester transfer protein concentrations in Chinese women显示文摘 | Zhang CN Zhuang YY Qiang HJ Liu XC Xu RJ Wu YZ | 2001 | Clin Chim Acta2001，314，： | 1 |
| 11 | Serum tartrate-resis-tantacid phosphatase5b activity as a prognostic marker of surviva-lin breast cancer with bone metastasis显示文摘 | Wu YY Janckila AJ Ku CH | 2010 | BMC Cancer2010，23，10： | 1 |
| 12 | Radiation therapy concurrent with weekly paclitaxel for locore gionally advanced nasopharyngeal carcinoma: outcomes of a phase I trial显示文摘 | Chen M Wu SX Chen YY | 2004 | Am J Clip Oncol2004，27，5： | 1 |
| 13 | Toxicologic effects of gold nanoparticles in vivoby different administration routes显示文摘 | Zhang XD Wu HY Wu D Wang YY Chang JH ZhaiZB | 2010 | Int J Nanomed2010，5，： | 1 |
| 14 | CD64 expression is increased in patients with severe acute pancreatitis: clinical significance 显示文摘 | Zhang H Ling XL Wu YY | 2014 | Gut Liver2014，8，4： | 1 |
| 15 | Treatment and complications of thalassemia major in Guangxi,Southern China显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，： | 1 |
| 16 | Functional alpha(1)- adrenoceptor subtypes in human submandibular glands显示文摘 | Huang Z Wu LL Zhang YY | 2006 | J Dent Res2006，85，3： | 1 |
| 17 | The relationship between ulcer recurrence and Helicobacter pylori: A prospective one-year follow up study in China 显示文摘 | Si JM Jin YY Wu JG | 2000 | Journal of Zhejiang University SC1ENC EA2000，1，2： | 1 |
| 18 | Treatment and compli- cations of thalassemia major in Guangxi, Southern China 显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，7： | 1 |
| 19 | Prevalence of dry eye among adult Chinese in the Beijing Eye Study显示文摘 | Jie Y L Xu YY Wu | 2009 | Eye CLond)2009，23，3： | 1 |
| 20 | TNF-related apoptosis-inducing ligand (TRAIL) induces osteoclast differentiation from monocyte/macrophage lineage precursor cells显示文摘 | Yen ML Tsai HF Wu YY | 2008 | Mol Immunol2008，45，8： | 1 |
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