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| 1 | An Improved Test Method and Numerical Analysis for Crack Opening Resistance of FRC Round Determinate Panels显示文摘This paper presents a numerical analysis of a material test program investigating the crack opening properties of fibre reinforced concrete (FRC) round determinate panels (RDP). The objective of this research is to set up a modified RDP test method to improve the current ASTM C1550 test method for FRC and fibre reinforced shotcrete (FRS) composite. By this test method, light small-diameter panels are applied and more crack informa-tion can be obtained by a new rotation angle measuring technique. It is shown that this modified test method can be used to effec-tively evaluate the crack opening resistance of FRC. The finite element analysis was then performed to clarify the crack propaga-tions and failure mode of FRC RDP panels. It helps establish a reasonable theoretical method to predict the structural response of RDP, combining with this modified testing technique. | GU Qian XU Hanfeng MINDESS Sidney | 2009 | Wuhan University Journal of Natural Sciences2009,14,1: | 2 |
| 2 | Integration of full-length transcriptomics and targeted metabolomics to identify benzylisoquinoline alkaloid biosynthetic genes in Corydalis yanhusuo显示文摘Corydalis yanhusuo W.T.Wang is a classic herb that is frequently used in traditional Chinese medicine and is efficacious in promoting blood circulation,enhancing energy,and relieving pain.Benzylisoquinoline alkaloids(BIAs)are the main bioactive ingredients in Corydalis yanhusuo.However,few studies have investigated the BIA biosynthetic pathway in C.yanhusuo,and the biosynthetic pathway of species-specific chemicals such as tetrahydropalmatine remains unclear.We performed full-length transcriptomic and metabolomic analyses to identify candidate genes that might be involved in BIA biosynthesis and identified a total of 101 full-length transcripts and 19 metabolites involved in the BIA biosynthetic pathway.Moreover,the contents of 19 representative BIAs in C.yanhusuo were quantified by classical targeted metabolomic approaches.Their accumulation in the tuber was consistent with the expression patterns of identified BIA biosynthetic genes in tubers and leaves,which reinforces the validity and reliability of the analyses.Full-length genes with similar expression or enrichment patterns were identified,and a complete BIA biosynthesis pathway in C.yanhusuo was constructed according to these findings.Phylogenetic analysis revealed a total of ten enzymes that may possess columbamine-O-methyltransferase activity,which is the final step for tetrahydropalmatine synthesis.Our results span the whole BIA biosynthetic pathway in C.yanhusuo.Our full-length transcriptomic data will enable further molecular cloning of enzymes and activity validation studies. | Dingqiao Xu Hanfeng Lin Yuping Tang Lu Huang Jian Xu Sihui Nian Yucheng Zhao | 2021 | Horticulture Research2021,8,1: | 2 |
| 3 | Analytical model for concrete confined with fiber reinforced polymer composite显示文摘 | FUJIKAKE K MINDESS S XU Hanfeng | 2004 | Journal of Composites for Construction2004,8,4: | 1 |
| 4 | Analytical model for concrete confined with fiber reinforced polymer composite 显示文摘 | Kazunori Fujikake Sidney Mindess Xu Hanfeng | 2004 | Journal of Composites for Construction2004,8,4: | 1 |
| 5 | Analytical model for concrete confined with fiber reinforced polymer composite 显示文摘 | KAZUNORI FUJIKAKE SIDNEY MINDESS XU HANFENG | 2004 | Journal of Composites for Construction2004,8,4: | 1 |
| 6 | Simultaneous measurement of force and temperature with a single FBG partially encapsulated with a metal cannula显示文摘 | Yang Zhangcheng Xu Hanfeng Ni Kai | 2011 | Microwave Optical Technology Letter2011,53,7: | 1 |
| 7 | Analyti cal model for concrete confined with fiber reinforced polymer composite 显示文摘 | FUJIKAKE K MINDESS S XU Hanfeng | 2004 | ASCE Journal of Composites for Construction2004,8,4: | 1 |
| 8 | Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea(Pisum sativum L.)显示文摘Pea(Pisum sativum L.)is an annual cool-season legume crop.Owing to its role in sustainable agriculture as both a rotation and a cash crop,its global market is expanding and increased production is urgently needed.For both technical and regulatory reasons,neither conventional nor transgenic breeding techniques can keep pace with the demand for increased production.In answer to this challenge,CRISPR/Cas9 genome editing technology has been gaining traction in plant biology and crop breeding in recent years.However,there are currently no reports of the successful application of the CRISPR/Cas9 genome editing technology in pea.We developed a transient transformation system of hairy roots,mediated by Agrobacterium rhizogenes strain K599,to validate the efficiency of a CRISPR/Cas9 system.Further optimization resulted in an efficient vector,PsU6.3-tRNA-PsPDS3-en35S-PsCas9.We used this optimized CRISPR/Cas9 system to edit the pea phytoene desaturase(PsPDS)gene,causing albinism,by Agrobacterium-mediated genetic transformation.This is the first report of successful generation of gene-edited pea plants by this route. | Guan Li Rong Liu Rongfang Xu Rajeev KVarshney Hanfeng Ding Mengwei Li Xin Yan Shuxian Huang Juan Li Dong Wang Yishan Ji Chenyu Wang Junguang He Yingfeng Luo Shenghan Gao Pengcheng Wei Xuxiao Zong Tao Yang | 2023 | The Crop Journal2023,11,1: | 0 |
| 9 | 基于裂缝宽度量测技术的FRC材料小尺寸圆板试验方法显示文摘在美国材料试验协会标准ASTM C1550圆板试验方法基础上,提出一种改进的基于裂缝宽度量测技术的纤维混凝土(FRC)材料静定圆板试验方法。该试验方法采用易于现场制作和试验的小尺寸圆板,并运用加载过程中板的径向滑移和转动角度量测技术,通过合理假定和数学推导得到板底中心裂缝宽度值,获得更多板在裂后阶段大变形下有关裂缝开展的实测数据,从而有效地评定纤维混凝土材料的弯曲韧性、延性等材料性能。同时应用有限元方法,分析试验圆板的裂缝开展特点及破坏形态,对比试验研究结果,验证该试验方法为获得裂缝宽度值所做的合理假定,为建立FRC静定圆板的理论计算方法提供依据。 | 谷倩 Xu Hanfeng Sidney Mindess | 2008 | 华中科技大学学报(城市科学版)2008,25,3: | 0 |