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| 1 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 2 | Assessing insomnia in adole- scents: comparison of Insomnia Severity Index, Athens Insomnia Scale and Sleep Quality Index显示文摘 | Chung KF Kan KK Yeung WF | 2011 | Sleep Med2011，12，5： | 1 |
| 3 | Hyperhomocysteinemia ac- tivates nuclear factor-kB in endothelial cells via oxidative stress 显示文摘 | An Yeung KK Woo CW Sung FL | 2004 | Circ Res2004，94，： | 1 |
| 4 | Prevalence of contact lens-related complications: UCLA contact lens study显示文摘 | FORISTER JF FORISTER EF YEUNG KK | 2009 | Eye Contact Lens2009，35，4： | 1 |
| 5 | A systematic review of endovascular treatment of extensive aortoiliac occlusive disease 显示文摘 | Jongkind V Akkersdijk GJ Yeung KK | 2010 | J Vasc Surg2010，52，5： | 1 |
| 6 | Assessing insomnia in adolescents : Comparison of Insomnia Severity Index, Athens Insomnia Scale and Sleep Quality Index显示文摘 | Chung KF Kan KK Yeung WF | 2011 | Sleep Med2011，12，5： | 1 |
| 7 | Hyperhomocysteinemia Activates Nuclear Factor-kB in Endothelial Cells via Oxidative Stress显示文摘 | Au - Yeung KK Woo CW Sung FL | 2004 | Circ Res2004，94，： | 1 |
| 8 | Routine continuous cold perfusion of the kidneys during elective juxtarenal aortic aneurysm repair显示文摘 | Yeung KK Jongkind V Coveliers HM | 2008 | Eur J Vasc Endovasc Surg2008，35，4： | 1 |
| 9 | A systematic review of endovascular treatment of extensive aortoiliac occlusive disease显示文摘 | Jongkind V Akkersdijk GJ Yeung KK | 2010 | J Vasc Surg2010，52，5： | 1 |
| 10 | Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation 显示文摘 | Chan JF Chan KH Choi GK To KK Tse H Cai JP Yeung ML Cheng VC Chen H Che XY Lau SK Woo PC Yuen KY | 2013 | J Infect Dis2013，207，11： | 1 |
| 11 | Assessing insomnia in adoles- cents: comparison of Insomnia Severity Index, Athens Insomnia Scale and Sleep Quality Index显示文摘 | Chung KF Kan KK Yeung WF | 2011 | Sleep Med2011，12，5： | 1 |
| 12 | Homocysteine stimulatesphosphorylation of NADPH oxidase p47phox and p67phoxsubunits in monocytes via protein kinase Cbeta activation 显示文摘 | Siow YL Au - Yeung KK Woo CW | 2006 | Biochem J2006，398，1： | 1 |
| 13 | Hyperhomocysteine- miaactivates nuclear factor - kappaB in endothelial cells via oxida- tivestress 显示文摘 | Au - Yeung KK Woo CW Sung FL | 2004 | CircRes2004，94，1： | 1 |
| 14 | New post-imaging software provides fast and accurate volume data from CTA surveillance after endovascular aneurysm repair 显示文摘 | Yeung KK van der Laan MJ Wever JJ | 2003 | J Endovasc Ther2003，10，5： | 1 |
| 15 | A systematic review of endovascular treatment of extensive aortoiliac occlusive disease显示文摘 | Jongkind V Akkersdijk GJ Yeung KK | 2010 | J Vasc Surg2010，52，5： | 1 |
| 16 | Improved patency of infrainguinal polytetrafluoroethylene bypass grafts using a distal Taylor vein patch显示文摘 | Yeung KK Mills JL Hughes JD | 2001 | Am J Surg2001，182，6： | 1 |
| 17 | Impact of mood disturbance, sleep disturb- ance, fatigue and pain among patients receiving cancer therapy 显示文摘 | Cheng KK Yeung RM | 2013 | Eur J Cancer Care (Engl)2013，22，1： | 1 |
| 18 | Hyperhomocysteinemia ac?tivates nuclear factor - κB in endothelial cells via oxidative stress显示文摘 | Au -Yeung KK Woo CW Sung FL | 2004 | Cite Res2004，94，4： | 1 |
| 19 | A comparison of fluoracaine and fluorox on corneal epithelial cell desquamation after goldmann applanation tonometry显示文摘 | Yeung KK Kageyama JY Carnevali T | | 0，，01： | 1 |
| 20 | A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants显示文摘 | Wong HM Yeung KK Lam KO | 2010 | Biomaterials2010，31，8： | 1 |
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