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| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | The relationship of Imp2 and DR3 genes with susceptibility to type Ⅰ diabetes mellitus in south China Han population显示文摘AIN To study the relationship of Imp2 and DR3genes with type Ⅰ diabetes mellitus.NETHODS Imp2 genotypes and DR3 wereidentified in 68 patients with type Ⅰ diabetesmellitus(Ⅰ-DM)and 71 healthy controls.Then,Ⅰ-DM patients and controls were respectivelyallocated into DR3-positive and DR3-negativegroups.The frequencies of Imp2 and DR3 genein random subjects,and Imp2 genotypes in DR3-matched subjects were compared between Ⅰ-DMpatients and controls.At the same time,Ⅰ-DMpatients were divided into 3 groups based on theonset age of diabetics:group A≤14 years,group B 15-30 years and group C≥31 years.RESULTS The frequency of DR3 in Ⅰ-DMpatients was significantly higher than that incontrols(47% vs 21%,P<0.005),and it wassignificantly higher in group A than that in groupB+C(70% vs 36%,x^2=7.07,P<0.01).Therewas a significant difference among groups withdifferent onset age of diabetics(x^2=8.19,rp=0.33,P<0.05).In random subjects,thefrequency of Imp2.R/R in Ⅰ-DM patients waslower(43% vs 61%,P<0.05)and Imp2.R/Hhigher(53% vs 28%,P<0.05)than that incontrols,and there was no significant differenceamong groups with different onset age ofdiabetics.In DR3-positive subjects,thefrequency of Imp2.R/R in Ⅰ-DM patients waslower(47% vs 87%,P<0.05)and Imp2-R/H higher(47% vs 13%,P<0.05)than that incontrols.In DR3-negative subjects,thefrequency of Imp2.R/H in Ⅰ-DM patients washigher than that in controls(58% vs 32%,P<0.01),but the frequency of Imp2-R/R and Imp2H/H was not significantly different betweenthese two groups.CONCLUSION DR3 gene may be one of thesusceptible genes of Ⅰ-DM,and significantlyrelated to the onset age of diabetics,and thepersons with DR3 may have an younger onsetage of diabeteS.The Imp2-R/R may be theprotective genotype of Ⅰ-DM,and Imp2-R/H thesusceptible genotype.These were not affectedby DR3 gene.Imp-2 genotypes were not relatedwith the onset age of diabetics. | Ding HL Cheng H Fu ZZ Deng QL Yan L Yan T | 2000 | World Journal of Gastroenterology2000，6，1： | 7 |
| 3 | An eight-year study of epidemiologic features of enterovims 71 infection in Taiwan显示文摘 | Chen SO Chang HL Yan TB | 2007 | Am J Trop Med Hyg2007，77，1： | 1 |
| 4 | An eight-year study ofepidemiologic features of enterovirus 71 infection in Taiwan 显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J Trop Med Hyg2007，77，1： | 1 |
| 5 | An eight-year study of epi-demiologic features of enterovirus 71 infection in Taiwan显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J Trop Med Hyg2007，77，1： | 1 |
| 6 | An eight-year study of epidemiologic features of enterovirus 71 infection in Taiwan 显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J TropMed Hyg2007，77，1： | 1 |
| 7 | TGF-β stimalation and inhibition of cell proliferation: New mechanistic insights显示文摘 | MOSEL HL YAN EY PIETENPOL JA | 1990 | Cell1990，62，2： | 1 |
| 8 | Protective effects of soybean isoflavone against gamma irradiation induced damages in mice显示文摘 | Song LH Yan HL Cai DL | 2006 | J Radiat Res (Tokyo)2006，47，15： | 1 |
| 9 | An eight-year study of epidemiologic feature of enterovirus 71 infection in Taiwan显示文摘 | Chen SC Chang HL Yan TR | | 0，，01： | 1 |
| 10 | Aneight-year study of epide- miologic features of enterovirus 71 infection in Taiwan显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J Trop Hyg2007，77，1： | 1 |
| 11 | Genetic characterization of CRF01_AE full-length human immunodeficiency virus type 1 sequences from Fujian, China显示文摘 | Huang HL Zheng J Yan PP | 2007 | AIDS Res Hum Retroviruses2007，23，4： | 1 |
| 12 | An Eight-Year Study of Epidemiologic Features of Enterovirus 71 Infection In Taiwan显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J Trop Med Hyg2007，77，1： | 1 |
| 13 | An eight - year study of epide- miologic features of enterovirus 71 infection in Taiwan显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J Trop Med Hyg2007，77，1： | 1 |
| 14 | Aneuploid analysis of tripronuclear zygotes derived from in vitro fertilization and intracytoplasmic sperm injection in humans显示文摘 | Chen Z Yan J Feng HL | 2005 | Fertil Steril2005，83，6： | 1 |
| 15 | Time course of upregulation of fibrogenic growth factors and cellular infiltration in a rodent model of chronic renal allograft rejection显示文摘 | Pilmore HL Yan Y Eris JM | 2002 | Transplant Immunology2002，10，： | 1 |
| 16 | A mechanism underlying hy- pertensive occurrence in the metabolic syndrome: cooperative effect of oxidative stress and Calcium accumulation in vascular smooth mus- cle cells显示文摘 | Zhang X Yan SM Zheng HL | 2014 | Horm Metab Res2014，46，2： | 1 |
| 17 | The validity and reliability of the self-directed learning instrument (SDLI) in mainland Chinese nursing students显示文摘 | Shen WQ Chen HL Yan H | 2014 | BMC Med Educ2014，14，108： | 1 |
| 18 | An eight -year study of epidemiologic features of enterovirus 71 infection in Taiwan显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J Trop Med Hyg2007，77，1： | 1 |
| 19 | An eight-year study of epidemiologic features of enterovirus 71 infection in Taiwan 显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J Trop Med Hyg2007，77，1： | 1 |
| 20 | An eight-year study of epidemiologic features of enterovirus 71 infection in Taiwan显示文摘 | Chen SC Chang HL Yan TR | 2007 | Am J Trop Med Hyg2007，77，1： | 1 |
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