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| 1 | Efficacies of β-L-D4A against Hepatitis B virus in 2.2.15 cells显示文摘AIM:To investigate the antiviral effect of beta-L-enantiomer of 2',3'-didehydro-2',3'-dideoxyadenosine (β-L-D4A) on 2.2.15 cells transfected with the hepatitis B virus (HBV) genome. METHODS:Lamivudine (3TC) as a positive control. Then, HBV DNA in treated 2.2.15 cells and the Hepatitis B surface antigen (HBsAg) in the culture supernatants were detected to determine the inhibitory effect of β-L-D4A. At the same time, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to detect the survival ratio of 2.2.15 cells. RESULTS:β-L-D4A has a dose-dependent inhibitory effect on HBV DNA replication;this effect was apparent when the concentration was above 1 mol/L. When β-L-D4A was at the highest concentration, 100 mol/L, the HBsAg inhibition ratio was above 50%. The Therapeutic index (TI) of β-L-D4A was above 2.1. CONCLUSION:β-L-D4A has a dose-dependent inhibitory effect on the replication of HBV DNA and the secretion of HBsAg at low toxicity. | Lin-Lin Gao, Xiao-Yan Wang, Ju-Sheng Lin, Ying-Hui Zhang, Yan Li, Institute of Liver Diseases, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China Author contributions:Gao LL and Wang XY contributed equally to this work Gao LL, Wang XY, Lin JS, Zhang YH, Li Y designed the research Gao LL and Wang XY performed the research Gao LL wrote the paper | 2008 | World Journal of Gastroenterology2008，14，8： | 7 |
| 2 | Dot immunogold filtration assay for rapid detection of anti-HAV IgM in Chinese显示文摘INTRODUCTION The hepatitis A virus specific immunoglobulin M(IgM)antibody is a specific serological marker forearly diagnosis of hepatitis A..At present,themethods used at home or abroad for detecting anti-HAV IgM are RIA,ELISA and SPHAI.The dotimmunogold combination assay that has beendeveloped since 1989 is a new technique with theproperty of simple and rapid immunologicaldetection,by using the red colloidal gold particles tolabel the antibodies as indicator,and the | Han FC Hou Y Yan XJ Xiao LY Guo YH | 2000 | World Journal of Gastroenterology2000，6，3： | 5 |
| 3 | Apolipoprotein B gene 3'VNTR polymorphism:association with plasma lipids and coronary heart disease in Han Chinese显示文摘 | Yan SK Song YH Zhu WL | 2006 | Clin Chern Lab Med2006，44，10： | 1 |
| 4 | Purification and characterization of an extcacellular β-glucosidase II with high hydrolysis and transglucosylation activities from Aspergillusniger显示文摘 | Yan TR Lin CL Lin YH | 1998 | Journat of Agricultural and Food Chemistry1998，46，2： | 1 |
| 5 | Continuous quality im- provement of nursing care: case study of a clinical pathway revision for cardiac catheterization显示文摘 | Yan YH Chen Y Kung CM et a! | 2011 | J Nurs Res2011，19，3： | 1 |
| 6 | Brain metabolite changes in subcortical regions after exposure to cuprizone for 6 weeks:potential implications for schizophrenia显示文摘 | Yan G Xuan YH Dai ZZ | 2015 | Neurochem Res2015，40，： | 1 |
| 7 | Silibinin attenuates mast cell-mediated anaphylaxis-like reactions 显示文摘 | Choi YH Yan GH | 2009 | Biol Pharm Bu112009，32，5： | 1 |
| 8 | Meconium induces expression of inducible NO synthase and activation of NF-B in tat alveolarm acrophages 显示文摘 | LI YH YAN ZQ Brauner A | 2001 | Pediatr Res2001，49，6： | 1 |
| 9 | Ellagic acid attenuates immunoglobulin E-mediated allergic response in mast cell 显示文摘 | Choi YH Yan GH | 2009 | Biol Pharm Bull2009，32，6： | 1 |
| 10 | Management of local residual primary lesion of nasopharyngeal carcinoma:Ⅱ result of prospective randomized trial on booster dose显示文摘 | Yan JH Xu GZ Hu YH | 1990 | Int J Radiat Oncol Biol Phys1990，18，2： | 1 |
| 11 | Radiation therapy of recurrent nasopharyngeal carcinoma显示文摘 | Yan HJ Hu YH Cu XZ | 1993 | Acta Radiologica Oncology1993，22，1： | 1 |
| 12 | Activation of macrophage nuclear factor-κB and induction of inducible nitric oxide synthase by LPS 显示文摘 | Li YH Yan Zq Annelie Braune | 2002 | Respir Res2002，3，1： | 1 |
| 13 | Radiation therapy of recurrent nasopharyngeal carcinoma-report on 219 patients 显示文摘 | Yan JH Hu YH Gu XZ | 1983 | Acta Radiol Oncol1983，22，1： | 1 |
| 14 | Depolarization induces NR2A tyrosinephosphorylation and neuronal apoptosis显示文摘 | Liu Y Yan JZ Gu YH | 2011 | Can J Neurol Sci2011，38，6： | 1 |
| 15 | Effect of air pollution on blood pressure,blood lipids,and blood sugar::a population-based approach显示文摘 | Chuang KJ Yan YH Cheng TJ | 2010 | J Occup Environ Med2010，52，3： | 1 |
| 16 | Mecomium induces expression of inducible NO Synthase and activation of NF-appaB in rat alveolar macrophages显示文摘 | Li YH Yan ZO Brauner A | 2001 | Pediatr Res2001，49，6： | 1 |
| 17 | Gastric glomus tumor: A hypervascular submucosal tumor on power doppler endosonography显示文摘 | Yan SL Yeh YH Chen CH | 2007 | J Clin Ultrasound2007，35，3： | 1 |
| 18 | Silibinin attenuates mast cell-mediated anaph-ylaxis-like reactions显示文摘 | Choi YH Yan GH | 2009 | Biol Pharm Bull2009，32，5： | 1 |
| 19 | Continuous quality improvement of nursing care : case study of a clinical pathway revision for cardiac catheterization显示文摘 | Yan YH Chen Y Kung CM | 2011 | J Nurs Res2011，19，3： | 1 |
| 20 | Effect of naphthalene acetic acid on adventitious root development and associated physiological changes in stem cutting of Hemarthria compressa 显示文摘 | Yan YH Li JL Zhang XQ | 2014 | PLoS One2014，9，3： | 1 |
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